Humulus japonicus latent virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Bromoviridae; Ilarvirus

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6JE42|Q6JE42_9BROM ATP-dependent helicase OS=Humulus japonicus latent virus OX=269213 PE=3 SV=1
MM1 pKa = 7.78ASFKK5 pKa = 11.0DD6 pKa = 3.25IVNALFASAQQKK18 pKa = 10.53LGLSVPLLTGDD29 pKa = 4.09KK30 pKa = 10.14IIVNMPSNQKK40 pKa = 10.72KK41 pKa = 10.2KK42 pKa = 9.17MEE44 pKa = 4.25LVAVPLLRR52 pKa = 11.84EE53 pKa = 3.75NVRR56 pKa = 11.84FSRR59 pKa = 11.84EE60 pKa = 3.8TLVVFLRR67 pKa = 11.84HH68 pKa = 5.85PCLANMLNQWEE79 pKa = 4.58FWAWEE84 pKa = 4.17FNEE87 pKa = 3.87EE88 pKa = 4.22LLPYY92 pKa = 8.73GCSVTRR98 pKa = 11.84VKK100 pKa = 10.42DD101 pKa = 3.17ICVDD105 pKa = 3.62GPYY108 pKa = 9.96EE109 pKa = 4.29DD110 pKa = 3.75KK111 pKa = 10.97SGYY114 pKa = 9.4FHH116 pKa = 7.74GSYY119 pKa = 9.77TYY121 pKa = 11.14AEE123 pKa = 4.04NSVYY127 pKa = 10.61VDD129 pKa = 3.94DD130 pKa = 4.9YY131 pKa = 11.52QVEE134 pKa = 4.65LCPLDD139 pKa = 5.5DD140 pKa = 4.19NMPPEE145 pKa = 4.19SVYY148 pKa = 11.11EE149 pKa = 3.99EE150 pKa = 5.15GEE152 pKa = 4.3CSSKK156 pKa = 10.39YY157 pKa = 9.95QEE159 pKa = 4.38VPPCEE164 pKa = 4.37DD165 pKa = 3.13VNLDD169 pKa = 3.6GEE171 pKa = 4.27EE172 pKa = 4.45GEE174 pKa = 4.62NVGLLDD180 pKa = 3.84MPEE183 pKa = 4.77KK184 pKa = 10.74DD185 pKa = 4.16FQSLMHH191 pKa = 6.49TRR193 pKa = 11.84LQHH196 pKa = 6.23LRR198 pKa = 11.84DD199 pKa = 3.77VGCSINLSGRR209 pKa = 11.84GIPVIPIVAGGFSEE223 pKa = 4.39TTRR226 pKa = 11.84LNPRR230 pKa = 11.84HH231 pKa = 6.15AEE233 pKa = 3.9SEE235 pKa = 4.34RR236 pKa = 11.84VFKK239 pKa = 10.92DD240 pKa = 3.01RR241 pKa = 11.84LEE243 pKa = 4.32GPEE246 pKa = 4.61LKK248 pKa = 9.83WAGPVFKK255 pKa = 10.72DD256 pKa = 3.12NLRR259 pKa = 11.84AHH261 pKa = 6.63SVDD264 pKa = 4.03DD265 pKa = 3.39MDD267 pKa = 3.47VRR269 pKa = 11.84EE270 pKa = 4.22WVAINRR276 pKa = 11.84PIPKK280 pKa = 9.42KK281 pKa = 9.81KK282 pKa = 10.55LPMKK286 pKa = 10.41ISGSIDD292 pKa = 3.31APLLNVRR299 pKa = 11.84EE300 pKa = 4.11VSPVLNLVNNYY311 pKa = 8.83IPKK314 pKa = 9.56YY315 pKa = 10.9QMLDD319 pKa = 2.58WHH321 pKa = 6.75ALAKK325 pKa = 10.23FINNYY330 pKa = 6.6QAKK333 pKa = 8.76EE334 pKa = 3.86KK335 pKa = 10.78PMDD338 pKa = 3.25IKK340 pKa = 10.12ITSRR344 pKa = 11.84GFANRR349 pKa = 11.84HH350 pKa = 4.6VFQQALDD357 pKa = 4.29EE358 pKa = 4.65ILPHH362 pKa = 5.72HH363 pKa = 6.83HH364 pKa = 6.89KK365 pKa = 10.66FDD367 pKa = 4.29DD368 pKa = 4.01SFFQTMVEE376 pKa = 4.29CDD378 pKa = 4.32DD379 pKa = 3.95ISIEE383 pKa = 4.59LSRR386 pKa = 11.84CRR388 pKa = 11.84LDD390 pKa = 4.45LSVFNSWEE398 pKa = 3.9KK399 pKa = 11.01GSNKK403 pKa = 8.76VKK405 pKa = 10.5PMINSGVANRR415 pKa = 11.84RR416 pKa = 11.84FSTQRR421 pKa = 11.84EE422 pKa = 3.93AMLAVQKK429 pKa = 11.12RR430 pKa = 11.84NMNVPNLAHH439 pKa = 6.36CTDD442 pKa = 3.45IPAVVAEE449 pKa = 4.36VVDD452 pKa = 4.1KK453 pKa = 10.29FFKK456 pKa = 11.01VIIDD460 pKa = 3.85PDD462 pKa = 3.91KK463 pKa = 11.12LSQFPGFISEE473 pKa = 4.48GEE475 pKa = 3.76LAYY478 pKa = 10.88FEE480 pKa = 5.83DD481 pKa = 4.68YY482 pKa = 11.27LRR484 pKa = 11.84GKK486 pKa = 10.12QPDD489 pKa = 3.89PEE491 pKa = 4.2MLKK494 pKa = 10.86DD495 pKa = 3.54PLCFQRR501 pKa = 11.84LDD503 pKa = 3.57KK504 pKa = 10.38YY505 pKa = 11.2RR506 pKa = 11.84HH507 pKa = 4.36MMKK510 pKa = 10.86NNMKK514 pKa = 9.92PVEE517 pKa = 4.07DD518 pKa = 4.01HH519 pKa = 6.52SLNSEE524 pKa = 4.06RR525 pKa = 11.84ALMSTITYY533 pKa = 9.29HH534 pKa = 6.17EE535 pKa = 5.21KK536 pKa = 9.08GTIMSTSPIFLMAANRR552 pKa = 11.84ILMCLSDD559 pKa = 3.95KK560 pKa = 11.27VKK562 pKa = 10.6IPTGKK567 pKa = 9.18YY568 pKa = 8.52HH569 pKa = 7.04QLFSLDD575 pKa = 3.27AHH577 pKa = 5.63VLKK580 pKa = 10.61HH581 pKa = 4.96VKK583 pKa = 8.67HH584 pKa = 5.87WKK586 pKa = 10.21EE587 pKa = 3.22IDD589 pKa = 3.37FSKK592 pKa = 10.59FDD594 pKa = 3.37KK595 pKa = 11.19SQGEE599 pKa = 4.05LHH601 pKa = 6.94HH602 pKa = 5.92YY603 pKa = 7.97LQRR606 pKa = 11.84EE607 pKa = 3.69IFIRR611 pKa = 11.84IGVPMNFIDD620 pKa = 3.32AWVPQVTRR628 pKa = 11.84SFISDD633 pKa = 3.77PSNGLFFNVDD643 pKa = 3.8FQRR646 pKa = 11.84RR647 pKa = 11.84TGDD650 pKa = 2.74ACTYY654 pKa = 10.46LGNTLVTLCVLCHH667 pKa = 6.3VYY669 pKa = 10.92DD670 pKa = 4.55LGDD673 pKa = 3.96ANVLSVVASGDD684 pKa = 3.36DD685 pKa = 3.74CLIGSYY691 pKa = 10.52EE692 pKa = 4.25KK693 pKa = 10.71LDD695 pKa = 3.36QSRR698 pKa = 11.84EE699 pKa = 4.13YY700 pKa = 11.15LCSTLFNFEE709 pKa = 4.4AKK711 pKa = 10.25FPYY714 pKa = 9.38NQPFICSKK722 pKa = 10.29FLIIAEE728 pKa = 4.1MMDD731 pKa = 3.01GRR733 pKa = 11.84EE734 pKa = 3.94EE735 pKa = 4.29VIAVPNPIKK744 pKa = 10.72LLIKK748 pKa = 10.4LGSKK752 pKa = 10.36LNPEE756 pKa = 5.23DD757 pKa = 3.61FDD759 pKa = 3.66EE760 pKa = 5.38WFLAWRR766 pKa = 11.84DD767 pKa = 3.8HH768 pKa = 5.65IHH770 pKa = 6.03YY771 pKa = 8.43FQNAHH776 pKa = 6.54IVDD779 pKa = 4.14QIVDD783 pKa = 4.01LVEE786 pKa = 3.87VRR788 pKa = 11.84TGVRR792 pKa = 11.84NTQCLRR798 pKa = 11.84LALEE802 pKa = 4.32SFPHH806 pKa = 5.64VFRR809 pKa = 11.84SKK811 pKa = 9.9KK812 pKa = 9.7AVMRR816 pKa = 11.84EE817 pKa = 3.97FFCMEE822 pKa = 4.44EE823 pKa = 3.86IEE825 pKa = 4.47SIEE828 pKa = 4.04RR829 pKa = 11.84NRR831 pKa = 11.84QIKK834 pKa = 9.61AIKK837 pKa = 9.98ALNKK841 pKa = 9.86KK842 pKa = 9.9FKK844 pKa = 10.46LPRR847 pKa = 11.84STTHH851 pKa = 4.86VCC853 pKa = 3.58

Molecular weight: 98.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6JE40|Q6JE40_9BROM Movement protein OS=Humulus japonicus latent virus OX=269213 PE=4 SV=1
MM1 pKa = 7.54SNAFCASCGLRR12 pKa = 11.84SHH14 pKa = 7.36VGNCPPRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 9.97ASPGQKK29 pKa = 9.65NRR31 pKa = 11.84TRR33 pKa = 11.84IYY35 pKa = 9.49ALEE38 pKa = 4.06RR39 pKa = 11.84KK40 pKa = 8.8RR41 pKa = 11.84ANVRR45 pKa = 11.84VPPGTTLNPKK55 pKa = 9.88FFAQFSGYY63 pKa = 9.12NAEE66 pKa = 4.31EE67 pKa = 4.66CDD69 pKa = 5.09LLDD72 pKa = 5.05DD73 pKa = 5.03VINAIPSVSVSAASQKK89 pKa = 10.47RR90 pKa = 11.84VTEE93 pKa = 4.13AQLVVGDD100 pKa = 4.45PLKK103 pKa = 10.88GIQGPCTHH111 pKa = 6.78MGDD114 pKa = 3.43ATITARR120 pKa = 11.84TGGVRR125 pKa = 11.84GFYY128 pKa = 9.26MALDD132 pKa = 3.99ALLTGIPDD140 pKa = 3.5ATRR143 pKa = 11.84LKK145 pKa = 10.43SLVMHH150 pKa = 6.33ITFLSDD156 pKa = 3.39GGVLGLNTEE165 pKa = 4.23RR166 pKa = 11.84SDD168 pKa = 4.22QYY170 pKa = 11.77VEE172 pKa = 4.09PNPLKK177 pKa = 10.47RR178 pKa = 11.84KK179 pKa = 9.39RR180 pKa = 11.84FEE182 pKa = 4.03KK183 pKa = 10.49EE184 pKa = 3.8SPFWAQILFPDD195 pKa = 3.9NVKK198 pKa = 10.83LSDD201 pKa = 3.54VKK203 pKa = 11.17NYY205 pKa = 11.12AIMIKK210 pKa = 10.22FDD212 pKa = 3.64SDD214 pKa = 3.71YY215 pKa = 11.47SANTPLYY222 pKa = 10.16TRR224 pKa = 11.84EE225 pKa = 3.97CWVNHH230 pKa = 5.13YY231 pKa = 8.88QLPSAVIPEE240 pKa = 3.81EE241 pKa = 3.88AFARR245 pKa = 11.84KK246 pKa = 9.06

Molecular weight: 27.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2418

246

1041

604.5

68.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.692 ± 1.174

2.605 ± 0.148

6.245 ± 0.292

6.452 ± 0.416

4.839 ± 0.412

4.384 ± 0.362

2.481 ± 0.301

5.873 ± 0.252

7.155 ± 0.429

8.768 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.295

4.715 ± 0.331

4.715 ± 0.605

2.978 ± 0.249

5.624 ± 0.307

6.865 ± 0.259

4.549 ± 0.631

7.279 ± 0.194

1.034 ± 0.113

2.978 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski