Beijerinckiaceae bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Beijerinckiaceae; unclassified Beijerinckiaceae

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3557 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N8M7B3|A0A2N8M7B3_9RHIZ Uncharacterized protein (Fragment) OS=Beijerinckiaceae bacterium OX=1978229 GN=CR217_16820 PE=4 SV=1
MM1 pKa = 8.14DD2 pKa = 4.82SPAGAAPFVYY12 pKa = 9.14VTNSVSNTVSVIDD25 pKa = 3.68TATNTVVATIPAGVEE40 pKa = 3.72PAGVAVTPDD49 pKa = 3.1GKK51 pKa = 9.91HH52 pKa = 6.4AYY54 pKa = 9.24LANQGTLSVPGTTVLVIDD72 pKa = 3.9MTTNTVVATVGVGNFPSGVAITPGGTHH99 pKa = 6.09VYY101 pKa = 9.66VVNGFSNNVSVIDD114 pKa = 3.77TASNTVVGTPIPVGKK129 pKa = 10.04FPQSVALTPDD139 pKa = 3.38GTHH142 pKa = 5.92GFVVNEE148 pKa = 3.95FSNNVSVIDD157 pKa = 3.79TASNTMVGIPIPVGNFPIGVAITPNGTHH185 pKa = 7.07GYY187 pKa = 7.59VTNFDD192 pKa = 4.44DD193 pKa = 4.99NNVSVIDD200 pKa = 3.66TT201 pKa = 3.96

Molecular weight: 20.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N8MD69|A0A2N8MD69_9RHIZ Adenosine kinase OS=Beijerinckiaceae bacterium OX=1978229 GN=CR217_05575 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MASVGGRR28 pKa = 11.84KK29 pKa = 9.29VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3557

0

3557

927747

26

2626

260.8

28.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.837 ± 0.058

1.037 ± 0.016

5.26 ± 0.03

5.55 ± 0.049

3.981 ± 0.034

8.393 ± 0.042

2.163 ± 0.02

5.309 ± 0.029

3.728 ± 0.038

10.025 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.016

2.766 ± 0.026

5.515 ± 0.04

2.95 ± 0.026

7.472 ± 0.049

5.479 ± 0.028

5.0 ± 0.027

6.858 ± 0.036

1.319 ± 0.02

2.18 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski