Amino acid dipepetide frequency for Yersinia phage fEV-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.041AlaAla: 11.041 ± 2.264
0.654AlaCys: 0.654 ± 0.184
5.398AlaAsp: 5.398 ± 0.571
6.379AlaGlu: 6.379 ± 0.797
3.517AlaPhe: 3.517 ± 0.551
7.688AlaGly: 7.688 ± 0.922
2.045AlaHis: 2.045 ± 0.544
5.48AlaIle: 5.48 ± 0.662
4.089AlaLys: 4.089 ± 0.565
7.77AlaLeu: 7.77 ± 0.951
2.617AlaMet: 2.617 ± 0.602
2.372AlaAsn: 2.372 ± 0.498
5.316AlaPro: 5.316 ± 2.217
2.781AlaGln: 2.781 ± 0.573
5.316AlaArg: 5.316 ± 0.805
4.335AlaSer: 4.335 ± 0.655
5.889AlaThr: 5.889 ± 1.14
8.506AlaVal: 8.506 ± 1.092
1.227AlaTrp: 1.227 ± 0.371
1.636AlaTyr: 1.636 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.491CysAla: 0.491 ± 0.258
0.164CysCys: 0.164 ± 0.127
0.654CysAsp: 0.654 ± 0.189
0.491CysGlu: 0.491 ± 0.217
0.245CysPhe: 0.245 ± 0.137
0.409CysGly: 0.409 ± 0.169
0.245CysHis: 0.245 ± 0.137
0.409CysIle: 0.409 ± 0.173
0.409CysLys: 0.409 ± 0.175
0.9CysLeu: 0.9 ± 0.269
0.409CysMet: 0.409 ± 0.167
0.573CysAsn: 0.573 ± 0.265
0.164CysPro: 0.164 ± 0.111
0.327CysGln: 0.327 ± 0.177
0.9CysArg: 0.9 ± 0.269
0.573CysSer: 0.573 ± 0.215
0.736CysThr: 0.736 ± 0.248
0.245CysVal: 0.245 ± 0.126
0.164CysTrp: 0.164 ± 0.102
0.164CysTyr: 0.164 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
6.87AspAla: 6.87 ± 0.803
0.409AspCys: 0.409 ± 0.158
4.253AspAsp: 4.253 ± 0.652
3.844AspGlu: 3.844 ± 0.655
2.944AspPhe: 2.944 ± 0.423
5.97AspGly: 5.97 ± 0.622
1.145AspHis: 1.145 ± 0.314
2.944AspIle: 2.944 ± 0.42
2.126AspLys: 2.126 ± 0.526
4.907AspLeu: 4.907 ± 0.704
1.554AspMet: 1.554 ± 0.366
2.944AspAsn: 2.944 ± 0.554
3.026AspPro: 3.026 ± 0.487
2.208AspGln: 2.208 ± 0.341
3.599AspArg: 3.599 ± 0.465
4.008AspSer: 4.008 ± 0.688
3.435AspThr: 3.435 ± 0.499
4.989AspVal: 4.989 ± 0.686
1.227AspTrp: 1.227 ± 0.33
2.535AspTyr: 2.535 ± 0.501
0.0AspXaa: 0.0 ± 0.0
Glu
6.134GluAla: 6.134 ± 0.77
0.409GluCys: 0.409 ± 0.186
2.781GluAsp: 2.781 ± 0.47
3.762GluGlu: 3.762 ± 0.63
2.863GluPhe: 2.863 ± 0.446
3.517GluGly: 3.517 ± 0.533
1.881GluHis: 1.881 ± 0.424
2.863GluIle: 2.863 ± 0.673
2.535GluLys: 2.535 ± 0.529
6.625GluLeu: 6.625 ± 0.898
2.372GluMet: 2.372 ± 0.581
2.372GluAsn: 2.372 ± 0.606
1.799GluPro: 1.799 ± 0.432
3.762GluGln: 3.762 ± 0.607
3.926GluArg: 3.926 ± 0.519
3.517GluSer: 3.517 ± 0.602
3.108GluThr: 3.108 ± 0.669
4.089GluVal: 4.089 ± 0.614
0.654GluTrp: 0.654 ± 0.226
2.045GluTyr: 2.045 ± 0.325
0.0GluXaa: 0.0 ± 0.0
Phe
3.353PheAla: 3.353 ± 0.557
0.491PheCys: 0.491 ± 0.175
3.435PheAsp: 3.435 ± 0.478
2.617PheGlu: 2.617 ± 0.509
1.145PhePhe: 1.145 ± 0.295
2.617PheGly: 2.617 ± 0.461
0.654PheHis: 0.654 ± 0.281
2.617PheIle: 2.617 ± 0.426
2.208PheLys: 2.208 ± 0.38
2.372PheLeu: 2.372 ± 0.361
0.736PheMet: 0.736 ± 0.252
1.718PheAsn: 1.718 ± 0.342
1.39PhePro: 1.39 ± 0.407
1.309PheGln: 1.309 ± 0.216
1.799PheArg: 1.799 ± 0.339
1.718PheSer: 1.718 ± 0.32
2.535PheThr: 2.535 ± 0.435
2.29PheVal: 2.29 ± 0.559
0.491PheTrp: 0.491 ± 0.236
1.145PheTyr: 1.145 ± 0.333
0.0PheXaa: 0.0 ± 0.0
Gly
7.115GlyAla: 7.115 ± 0.809
0.654GlyCys: 0.654 ± 0.263
5.398GlyAsp: 5.398 ± 0.799
3.19GlyGlu: 3.19 ± 0.553
3.68GlyPhe: 3.68 ± 0.65
5.153GlyGly: 5.153 ± 0.803
1.636GlyHis: 1.636 ± 0.386
4.825GlyIle: 4.825 ± 0.603
4.171GlyLys: 4.171 ± 0.583
5.97GlyLeu: 5.97 ± 0.801
1.799GlyMet: 1.799 ± 0.588
3.271GlyAsn: 3.271 ± 0.549
0.654GlyPro: 0.654 ± 0.266
4.008GlyGln: 4.008 ± 0.704
3.762GlyArg: 3.762 ± 0.421
3.19GlySer: 3.19 ± 0.523
4.662GlyThr: 4.662 ± 0.7
5.889GlyVal: 5.889 ± 0.724
1.963GlyTrp: 1.963 ± 0.465
2.29GlyTyr: 2.29 ± 0.416
0.0GlyXaa: 0.0 ± 0.0
His
1.963HisAla: 1.963 ± 0.493
0.573HisCys: 0.573 ± 0.24
1.718HisAsp: 1.718 ± 0.387
1.39HisGlu: 1.39 ± 0.335
0.573HisPhe: 0.573 ± 0.207
1.718HisGly: 1.718 ± 0.438
0.9HisHis: 0.9 ± 0.262
0.9HisIle: 0.9 ± 0.285
1.063HisLys: 1.063 ± 0.268
1.309HisLeu: 1.309 ± 0.345
0.9HisMet: 0.9 ± 0.306
1.145HisAsn: 1.145 ± 0.286
1.063HisPro: 1.063 ± 0.302
0.736HisGln: 0.736 ± 0.24
1.309HisArg: 1.309 ± 0.353
1.227HisSer: 1.227 ± 0.3
1.799HisThr: 1.799 ± 0.606
1.227HisVal: 1.227 ± 0.287
0.573HisTrp: 0.573 ± 0.204
0.573HisTyr: 0.573 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
3.926IleAla: 3.926 ± 0.75
0.327IleCys: 0.327 ± 0.161
5.071IleAsp: 5.071 ± 0.567
4.335IleGlu: 4.335 ± 0.472
1.063IlePhe: 1.063 ± 0.306
4.008IleGly: 4.008 ± 0.625
1.145IleHis: 1.145 ± 0.323
3.517IleIle: 3.517 ± 0.473
3.026IleLys: 3.026 ± 0.504
3.435IleLeu: 3.435 ± 0.535
1.227IleMet: 1.227 ± 0.266
4.171IleAsn: 4.171 ± 0.505
2.372IlePro: 2.372 ± 0.381
3.108IleGln: 3.108 ± 0.447
3.19IleArg: 3.19 ± 0.616
2.944IleSer: 2.944 ± 0.455
4.089IleThr: 4.089 ± 0.638
3.844IleVal: 3.844 ± 0.555
0.736IleTrp: 0.736 ± 0.256
1.39IleTyr: 1.39 ± 0.306
0.0IleXaa: 0.0 ± 0.0
Lys
4.008LysAla: 4.008 ± 0.498
0.245LysCys: 0.245 ± 0.143
2.29LysAsp: 2.29 ± 0.44
2.617LysGlu: 2.617 ± 0.521
1.636LysPhe: 1.636 ± 0.325
3.435LysGly: 3.435 ± 0.653
1.063LysHis: 1.063 ± 0.303
3.108LysIle: 3.108 ± 0.427
1.636LysLys: 1.636 ± 0.441
4.498LysLeu: 4.498 ± 0.626
2.045LysMet: 2.045 ± 0.44
1.309LysAsn: 1.309 ± 0.28
2.454LysPro: 2.454 ± 0.401
2.372LysGln: 2.372 ± 0.484
3.599LysArg: 3.599 ± 0.571
2.372LysSer: 2.372 ± 0.444
3.926LysThr: 3.926 ± 0.654
3.19LysVal: 3.19 ± 0.488
0.981LysTrp: 0.981 ± 0.296
1.145LysTyr: 1.145 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
8.424LeuAla: 8.424 ± 0.85
0.818LeuCys: 0.818 ± 0.242
5.316LeuAsp: 5.316 ± 0.606
6.052LeuGlu: 6.052 ± 0.6
1.963LeuPhe: 1.963 ± 0.45
3.926LeuGly: 3.926 ± 0.487
2.045LeuHis: 2.045 ± 0.405
4.825LeuIle: 4.825 ± 0.889
4.989LeuLys: 4.989 ± 0.602
6.543LeuLeu: 6.543 ± 0.709
2.29LeuMet: 2.29 ± 0.433
4.335LeuAsn: 4.335 ± 0.613
3.435LeuPro: 3.435 ± 0.532
4.008LeuGln: 4.008 ± 0.611
5.398LeuArg: 5.398 ± 0.591
3.844LeuSer: 3.844 ± 0.668
6.952LeuThr: 6.952 ± 0.59
4.089LeuVal: 4.089 ± 0.588
0.9LeuTrp: 0.9 ± 0.308
2.126LeuTyr: 2.126 ± 0.476
0.0LeuXaa: 0.0 ± 0.0
Met
2.372MetAla: 2.372 ± 0.484
0.245MetCys: 0.245 ± 0.17
1.636MetAsp: 1.636 ± 0.33
1.227MetGlu: 1.227 ± 0.313
1.39MetPhe: 1.39 ± 0.276
2.29MetGly: 2.29 ± 0.527
0.491MetHis: 0.491 ± 0.205
1.636MetIle: 1.636 ± 0.411
2.126MetLys: 2.126 ± 0.516
2.29MetLeu: 2.29 ± 0.465
1.063MetMet: 1.063 ± 0.3
1.554MetAsn: 1.554 ± 0.351
1.145MetPro: 1.145 ± 0.352
1.554MetGln: 1.554 ± 0.358
1.963MetArg: 1.963 ± 0.358
1.145MetSer: 1.145 ± 0.357
2.29MetThr: 2.29 ± 0.34
2.045MetVal: 2.045 ± 0.326
0.245MetTrp: 0.245 ± 0.17
0.736MetTyr: 0.736 ± 0.21
0.0MetXaa: 0.0 ± 0.0
Asn
4.171AsnAla: 4.171 ± 0.499
0.573AsnCys: 0.573 ± 0.229
2.617AsnAsp: 2.617 ± 0.478
2.535AsnGlu: 2.535 ± 0.333
1.799AsnPhe: 1.799 ± 0.482
4.744AsnGly: 4.744 ± 0.66
1.145AsnHis: 1.145 ± 0.277
1.881AsnIle: 1.881 ± 0.33
2.045AsnLys: 2.045 ± 0.375
3.844AsnLeu: 3.844 ± 0.59
1.227AsnMet: 1.227 ± 0.291
2.454AsnAsn: 2.454 ± 0.551
1.881AsnPro: 1.881 ± 0.319
1.718AsnGln: 1.718 ± 0.444
1.963AsnArg: 1.963 ± 0.403
2.617AsnSer: 2.617 ± 0.465
1.881AsnThr: 1.881 ± 0.41
3.026AsnVal: 3.026 ± 0.621
0.654AsnTrp: 0.654 ± 0.182
1.799AsnTyr: 1.799 ± 0.32
0.0AsnXaa: 0.0 ± 0.0
Pro
6.298ProAla: 6.298 ± 2.175
0.0ProCys: 0.0 ± 0.0
2.699ProAsp: 2.699 ± 0.43
3.026ProGlu: 3.026 ± 0.582
1.309ProPhe: 1.309 ± 0.4
2.045ProGly: 2.045 ± 0.412
1.309ProHis: 1.309 ± 0.377
1.881ProIle: 1.881 ± 0.441
1.472ProLys: 1.472 ± 0.431
2.863ProLeu: 2.863 ± 0.446
1.39ProMet: 1.39 ± 0.329
2.045ProAsn: 2.045 ± 0.355
1.636ProPro: 1.636 ± 0.422
2.045ProGln: 2.045 ± 0.434
1.554ProArg: 1.554 ± 0.338
1.636ProSer: 1.636 ± 0.394
2.372ProThr: 2.372 ± 0.531
3.517ProVal: 3.517 ± 0.479
0.491ProTrp: 0.491 ± 0.205
0.573ProTyr: 0.573 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
3.926GlnAla: 3.926 ± 0.533
0.245GlnCys: 0.245 ± 0.135
2.126GlnAsp: 2.126 ± 0.361
2.535GlnGlu: 2.535 ± 0.435
1.963GlnPhe: 1.963 ± 0.379
3.353GlnGly: 3.353 ± 0.525
0.9GlnHis: 0.9 ± 0.323
2.535GlnIle: 2.535 ± 0.492
2.29GlnLys: 2.29 ± 0.483
3.271GlnLeu: 3.271 ± 0.463
1.063GlnMet: 1.063 ± 0.319
1.472GlnAsn: 1.472 ± 0.351
2.208GlnPro: 2.208 ± 0.372
4.171GlnGln: 4.171 ± 0.775
3.026GlnArg: 3.026 ± 0.491
2.944GlnSer: 2.944 ± 0.543
1.881GlnThr: 1.881 ± 0.572
3.19GlnVal: 3.19 ± 0.508
0.736GlnTrp: 0.736 ± 0.195
2.126GlnTyr: 2.126 ± 0.504
0.0GlnXaa: 0.0 ± 0.0
Arg
4.335ArgAla: 4.335 ± 0.516
1.145ArgCys: 1.145 ± 0.306
3.844ArgAsp: 3.844 ± 0.613
3.599ArgGlu: 3.599 ± 0.619
2.29ArgPhe: 2.29 ± 0.499
4.498ArgGly: 4.498 ± 0.644
1.554ArgHis: 1.554 ± 0.466
3.353ArgIle: 3.353 ± 0.734
3.19ArgLys: 3.19 ± 0.572
4.744ArgLeu: 4.744 ± 0.544
2.617ArgMet: 2.617 ± 0.425
2.454ArgAsn: 2.454 ± 0.501
1.799ArgPro: 1.799 ± 0.361
2.454ArgGln: 2.454 ± 0.385
3.599ArgArg: 3.599 ± 0.657
2.454ArgSer: 2.454 ± 0.383
3.762ArgThr: 3.762 ± 0.527
3.435ArgVal: 3.435 ± 0.447
0.654ArgTrp: 0.654 ± 0.242
2.126ArgTyr: 2.126 ± 0.362
0.0ArgXaa: 0.0 ± 0.0
Ser
4.335SerAla: 4.335 ± 0.875
0.164SerCys: 0.164 ± 0.119
3.353SerAsp: 3.353 ± 0.465
2.29SerGlu: 2.29 ± 0.427
2.372SerPhe: 2.372 ± 0.495
4.58SerGly: 4.58 ± 0.494
0.491SerHis: 0.491 ± 0.183
3.353SerIle: 3.353 ± 0.519
2.29SerLys: 2.29 ± 0.33
4.335SerLeu: 4.335 ± 0.539
1.472SerMet: 1.472 ± 0.423
2.781SerAsn: 2.781 ± 0.338
2.454SerPro: 2.454 ± 0.31
1.963SerGln: 1.963 ± 0.398
2.372SerArg: 2.372 ± 0.362
3.19SerSer: 3.19 ± 0.588
3.026SerThr: 3.026 ± 0.378
5.48SerVal: 5.48 ± 0.643
0.491SerTrp: 0.491 ± 0.218
1.963SerTyr: 1.963 ± 0.378
0.0SerXaa: 0.0 ± 0.0
Thr
6.298ThrAla: 6.298 ± 0.847
0.409ThrCys: 0.409 ± 0.162
4.907ThrAsp: 4.907 ± 0.815
3.271ThrGlu: 3.271 ± 0.486
2.208ThrPhe: 2.208 ± 0.48
5.561ThrGly: 5.561 ± 0.67
1.963ThrHis: 1.963 ± 0.596
4.58ThrIle: 4.58 ± 0.499
3.108ThrLys: 3.108 ± 0.519
6.216ThrLeu: 6.216 ± 0.668
1.636ThrMet: 1.636 ± 0.444
2.126ThrAsn: 2.126 ± 0.416
2.944ThrPro: 2.944 ± 0.513
1.963ThrGln: 1.963 ± 0.394
2.454ThrArg: 2.454 ± 0.426
3.762ThrSer: 3.762 ± 0.594
2.863ThrThr: 2.863 ± 0.509
5.234ThrVal: 5.234 ± 0.738
0.818ThrTrp: 0.818 ± 0.279
1.309ThrTyr: 1.309 ± 0.356
0.0ThrXaa: 0.0 ± 0.0
Val
5.807ValAla: 5.807 ± 0.918
0.654ValCys: 0.654 ± 0.238
5.316ValAsp: 5.316 ± 0.665
5.234ValGlu: 5.234 ± 0.624
2.372ValPhe: 2.372 ± 0.467
4.008ValGly: 4.008 ± 0.531
1.227ValHis: 1.227 ± 0.324
3.762ValIle: 3.762 ± 0.514
3.026ValLys: 3.026 ± 0.629
6.216ValLeu: 6.216 ± 0.556
1.799ValMet: 1.799 ± 0.382
3.599ValAsn: 3.599 ± 0.62
2.781ValPro: 2.781 ± 0.517
3.19ValGln: 3.19 ± 0.455
4.58ValArg: 4.58 ± 0.715
3.926ValSer: 3.926 ± 0.749
5.807ValThr: 5.807 ± 0.923
6.461ValVal: 6.461 ± 0.582
1.145ValTrp: 1.145 ± 0.27
2.126ValTyr: 2.126 ± 0.366
0.0ValXaa: 0.0 ± 0.0
Trp
0.981TrpAla: 0.981 ± 0.281
0.164TrpCys: 0.164 ± 0.099
0.491TrpAsp: 0.491 ± 0.2
0.981TrpGlu: 0.981 ± 0.334
0.409TrpPhe: 0.409 ± 0.179
1.309TrpGly: 1.309 ± 0.319
0.409TrpHis: 0.409 ± 0.164
0.736TrpIle: 0.736 ± 0.247
0.818TrpLys: 0.818 ± 0.262
1.39TrpLeu: 1.39 ± 0.313
0.409TrpMet: 0.409 ± 0.167
1.063TrpAsn: 1.063 ± 0.378
0.409TrpPro: 0.409 ± 0.156
0.736TrpGln: 0.736 ± 0.304
1.063TrpArg: 1.063 ± 0.268
1.063TrpSer: 1.063 ± 0.332
0.491TrpThr: 0.491 ± 0.161
0.9TrpVal: 0.9 ± 0.271
0.409TrpTrp: 0.409 ± 0.24
0.491TrpTyr: 0.491 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.208TyrAla: 2.208 ± 0.398
0.327TyrCys: 0.327 ± 0.153
1.636TyrAsp: 1.636 ± 0.382
1.636TyrGlu: 1.636 ± 0.49
0.9TyrPhe: 0.9 ± 0.267
2.454TyrGly: 2.454 ± 0.443
0.409TyrHis: 0.409 ± 0.175
1.718TyrIle: 1.718 ± 0.406
1.309TyrLys: 1.309 ± 0.329
2.781TyrLeu: 2.781 ± 0.414
0.736TyrMet: 0.736 ± 0.214
0.818TyrAsn: 0.818 ± 0.239
1.227TyrPro: 1.227 ± 0.429
1.554TyrGln: 1.554 ± 0.423
2.535TyrArg: 2.535 ± 0.424
2.372TyrSer: 2.372 ± 0.391
2.126TyrThr: 2.126 ± 0.408
1.39TyrVal: 1.39 ± 0.359
0.164TyrTrp: 0.164 ± 0.111
1.145TyrTyr: 1.145 ± 0.302
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (12228 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski