Enterobacteriaceae endosymbiont of Macroplea appendiculata

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; unclassified Enterobacteriaceae

Average proteome isoelectric point is 8.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 415 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H2F945|A0A6H2F945_9ENTR Tryptophan synthase beta chain OS=Enterobacteriaceae endosymbiont of Macroplea appendiculata OX=2675790 GN=trpB PE=3 SV=1
MM1 pKa = 7.46SSSISEE7 pKa = 4.06RR8 pKa = 11.84VKK10 pKa = 10.85KK11 pKa = 10.54IIIEE15 pKa = 3.87QLGIQSKK22 pKa = 10.88DD23 pKa = 3.16IIEE26 pKa = 4.74DD27 pKa = 3.67ANFTKK32 pKa = 10.74DD33 pKa = 3.79LGADD37 pKa = 3.33SLDD40 pKa = 3.63TVEE43 pKa = 6.37LIMALEE49 pKa = 4.28EE50 pKa = 4.16EE51 pKa = 4.9FDD53 pKa = 4.09TEE55 pKa = 4.23ITDD58 pKa = 3.52EE59 pKa = 4.33DD60 pKa = 4.07AEE62 pKa = 4.73KK63 pKa = 9.64ITTVKK68 pKa = 10.74DD69 pKa = 3.73AIHH72 pKa = 6.61YY73 pKa = 10.22INNLYY78 pKa = 10.1NN79 pKa = 3.33

Molecular weight: 8.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H2F9R4|A0A6H2F9R4_9ENTR 50S ribosomal protein L9 OS=Enterobacteriaceae endosymbiont of Macroplea appendiculata OX=2675790 GN=rplI PE=3 SV=1
MM1 pKa = 6.67VTINQLVRR9 pKa = 11.84KK10 pKa = 8.64PRR12 pKa = 11.84TRR14 pKa = 11.84KK15 pKa = 8.0KK16 pKa = 8.96TKK18 pKa = 10.7SNVPALNSCPQKK30 pKa = 10.64RR31 pKa = 11.84GVCTKK36 pKa = 10.51VYY38 pKa = 7.04TTTPKK43 pKa = 10.53KK44 pKa = 10.26PNSALRR50 pKa = 11.84KK51 pKa = 7.38VCRR54 pKa = 11.84VRR56 pKa = 11.84LTNGYY61 pKa = 9.58EE62 pKa = 3.97VTSYY66 pKa = 10.91IAGEE70 pKa = 3.94GHH72 pKa = 6.34NLQEE76 pKa = 4.28HH77 pKa = 5.6SVVLIRR83 pKa = 11.84GGRR86 pKa = 11.84VKK88 pKa = 10.7DD89 pKa = 3.73LPGVRR94 pKa = 11.84YY95 pKa = 8.06HH96 pKa = 6.9TIRR99 pKa = 11.84GTLDD103 pKa = 3.05CSGVKK108 pKa = 9.43NRR110 pKa = 11.84KK111 pKa = 9.1KK112 pKa = 10.56GRR114 pKa = 11.84SKK116 pKa = 11.31YY117 pKa = 10.14GVKK120 pKa = 8.05QTKK123 pKa = 9.46

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

415

0

415

140920

29

1399

339.6

39.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.002 ± 0.09

1.355 ± 0.04

3.739 ± 0.066

3.593 ± 0.091

4.586 ± 0.083

4.74 ± 0.106

3.033 ± 0.063

14.377 ± 0.142

9.394 ± 0.105

9.715 ± 0.122

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.311 ± 0.042

8.672 ± 0.111

2.823 ± 0.046

4.128 ± 0.057

2.845 ± 0.074

5.343 ± 0.072

5.37 ± 0.05

4.042 ± 0.082

0.809 ± 0.037

5.124 ± 0.091
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski