Serratia phage vB_SspS_OS31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9V3X3|A0A7G9V3X3_9CAUD Uncharacterized protein OS=Serratia phage vB_SspS_OS31 OX=2769335 GN=phiOS31_p11 PE=4 SV=1
MM1 pKa = 7.64AFLAEE6 pKa = 4.76DD7 pKa = 4.47FAQAGEE13 pKa = 4.05EE14 pKa = 4.59GEE16 pKa = 4.67ANGLHH21 pKa = 6.12GWSDD25 pKa = 3.15AHH27 pKa = 6.59NVYY30 pKa = 10.44NDD32 pKa = 3.62CVSIVVDD39 pKa = 3.82HH40 pKa = 6.54VEE42 pKa = 4.59SPFYY46 pKa = 10.33WSGPGKK52 pKa = 10.06DD53 pKa = 3.19WRR55 pKa = 11.84LQPGLFNSEE64 pKa = 3.83VPAGVAVEE72 pKa = 4.15HH73 pKa = 6.89AGTIAMAEE81 pKa = 4.1FRR83 pKa = 11.84DD84 pKa = 4.31GSPALSAYY92 pKa = 9.21FPPSPAAAALGG103 pKa = 3.71

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9V3Z2|A0A7G9V3Z2_9CAUD Uncharacterized protein OS=Serratia phage vB_SspS_OS31 OX=2769335 GN=phiOS31_p30 PE=4 SV=1
MM1 pKa = 7.34VGRR4 pKa = 11.84LSSFKK9 pKa = 10.84AITTCRR15 pKa = 11.84QRR17 pKa = 11.84PLPPRR22 pKa = 11.84CFCFNAPTLSVTQRR36 pKa = 11.84ASTALILTWPPRR48 pKa = 11.84GKK50 pKa = 10.39KK51 pKa = 10.36LNTGQSSKK59 pKa = 10.71ISLRR63 pKa = 11.84AIWLCLPLLLVGCSTTRR80 pKa = 11.84TAYY83 pKa = 10.47VPAQCQPIPATLTQPVLAPLPPARR107 pKa = 4.78

Molecular weight: 11.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13074

45

1120

217.9

24.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.813 ± 0.52

1.254 ± 0.196

5.599 ± 0.246

6.173 ± 0.237

3.564 ± 0.205

7.702 ± 0.327

1.522 ± 0.171

5.561 ± 0.26

5.813 ± 0.31

7.894 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.146

4.352 ± 0.199

4.337 ± 0.312

4.482 ± 0.358

5.629 ± 0.209

6.524 ± 0.273

6.035 ± 0.389

6.211 ± 0.23

1.652 ± 0.15

3.151 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski