Hungateiclostridium straminisolvens JCM 21531

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Acetivibrio; Acetivibrio straminisolvens

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4383 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4V9U1|W4V9U1_9FIRM 50S ribosomal protein L29 OS=Hungateiclostridium straminisolvens JCM 21531 OX=1294263 GN=rpmC PE=3 SV=1
MM1 pKa = 7.58SIFNASDD8 pKa = 3.17GGILNDD14 pKa = 3.7YY15 pKa = 9.94MLFFDD20 pKa = 3.72WLDD23 pKa = 4.12RR24 pKa = 11.84ILSQEE29 pKa = 3.95LPKK32 pKa = 10.7GIKK35 pKa = 9.95AFNFNLYY42 pKa = 9.7EE43 pKa = 4.39GAEE46 pKa = 4.18GTYY49 pKa = 10.1DD50 pKa = 3.19IQLIGSDD57 pKa = 4.45EE58 pKa = 4.06FDD60 pKa = 4.23EE61 pKa = 6.34DD62 pKa = 4.77DD63 pKa = 5.26SDD65 pKa = 3.93WACTDD70 pKa = 4.34FFTTGEE76 pKa = 4.56DD77 pKa = 2.64ICFIKK82 pKa = 10.35RR83 pKa = 11.84SKK85 pKa = 10.51NIEE88 pKa = 3.82YY89 pKa = 10.01WEE91 pKa = 5.28DD92 pKa = 3.05GLKK95 pKa = 10.69YY96 pKa = 9.02ITEE99 pKa = 3.63IVMRR103 pKa = 11.84YY104 pKa = 9.06LDD106 pKa = 3.53EE107 pKa = 5.2GIYY110 pKa = 10.02VHH112 pKa = 6.64ILKK115 pKa = 10.65GADD118 pKa = 2.9AVGIGFVDD126 pKa = 3.87GDD128 pKa = 3.62IDD130 pKa = 5.03IIYY133 pKa = 10.2CSKK136 pKa = 9.98TVV138 pKa = 2.88

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4V7F6|W4V7F6_9FIRM Uncharacterized protein OS=Hungateiclostridium straminisolvens JCM 21531 OX=1294263 GN=JCM21531_2647 PE=4 SV=1
MM1 pKa = 7.5LRR3 pKa = 11.84TFQPKK8 pKa = 9.06KK9 pKa = 8.34RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.49EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 9.76RR21 pKa = 11.84MKK23 pKa = 8.31TANGRR28 pKa = 11.84KK29 pKa = 7.52VLRR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.26GRR39 pKa = 11.84KK40 pKa = 8.78VLSAA44 pKa = 4.05

Molecular weight: 5.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4383

0

4383

1081527

37

2354

246.8

27.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.295 ± 0.04

1.192 ± 0.017

5.745 ± 0.029

7.429 ± 0.046

4.321 ± 0.027

6.656 ± 0.038

1.442 ± 0.015

8.628 ± 0.043

7.957 ± 0.038

8.747 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.655 ± 0.022

5.439 ± 0.032

3.345 ± 0.025

2.569 ± 0.019

4.166 ± 0.029

6.369 ± 0.032

5.016 ± 0.033

6.908 ± 0.034

0.92 ± 0.014

4.201 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski