Hubei sobemo-like virus 45

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEI0|A0A1L3KEI0_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 45 OX=1923233 PE=4 SV=1
MM1 pKa = 6.19TQAMIPEE8 pKa = 5.11DD9 pKa = 3.86DD10 pKa = 4.03LRR12 pKa = 11.84EE13 pKa = 4.08KK14 pKa = 10.66VLSVMEE20 pKa = 4.59DD21 pKa = 2.87WYY23 pKa = 11.18KK24 pKa = 8.97PCRR27 pKa = 11.84WVLPDD32 pKa = 3.58DD33 pKa = 3.58WFSFEE38 pKa = 4.71RR39 pKa = 11.84FRR41 pKa = 11.84LVLNRR46 pKa = 11.84LDD48 pKa = 3.99RR49 pKa = 11.84SSSPGYY55 pKa = 8.91PYY57 pKa = 10.42CGYY60 pKa = 10.61KK61 pKa = 8.59PTIGEE66 pKa = 3.88WLGFDD71 pKa = 3.54GFVYY75 pKa = 10.64DD76 pKa = 5.06PIQVQLLWLDD86 pKa = 3.52VQRR89 pKa = 11.84VRR91 pKa = 11.84DD92 pKa = 4.04GEE94 pKa = 4.12SDD96 pKa = 3.34LVQRR100 pKa = 11.84VFIKK104 pKa = 10.63RR105 pKa = 11.84EE106 pKa = 3.62PHH108 pKa = 6.1KK109 pKa = 10.46KK110 pKa = 9.94SKK112 pKa = 10.22AAEE115 pKa = 4.23GRR117 pKa = 11.84WRR119 pKa = 11.84LIMAFPLDD127 pKa = 4.01HH128 pKa = 6.24QVFWHH133 pKa = 6.07MLFDD137 pKa = 3.87YY138 pKa = 11.24QNDD141 pKa = 4.0LEE143 pKa = 4.62ISHH146 pKa = 6.43ATSIPSQQGIWLHH159 pKa = 5.52GGAWRR164 pKa = 11.84GHH166 pKa = 4.16IQRR169 pKa = 11.84WKK171 pKa = 8.96QFGYY175 pKa = 10.88NVGLDD180 pKa = 3.33KK181 pKa = 11.27SAWDD185 pKa = 3.18WTYY188 pKa = 11.22PSWLLDD194 pKa = 2.85WDD196 pKa = 3.79LQFRR200 pKa = 11.84YY201 pKa = 10.48RR202 pKa = 11.84MGHH205 pKa = 4.84GSKK208 pKa = 9.43MEE210 pKa = 3.69EE211 pKa = 3.96WMNLASRR218 pKa = 11.84EE219 pKa = 4.03WKK221 pKa = 10.26LAFGVGAKK229 pKa = 10.39FITTQGWLLEE239 pKa = 4.14QQVPGVMKK247 pKa = 10.36SGAVVTIASNSHH259 pKa = 5.34AQAMLHH265 pKa = 6.04VLVCLDD271 pKa = 3.78EE272 pKa = 4.8GVDD275 pKa = 3.79YY276 pKa = 11.15EE277 pKa = 5.12PFPACCGDD285 pKa = 3.69DD286 pKa = 3.27TLQRR290 pKa = 11.84LDD292 pKa = 3.35QASVEE297 pKa = 4.62GYY299 pKa = 9.69AKK301 pKa = 10.55YY302 pKa = 10.65GVVVKK307 pKa = 10.47SASEE311 pKa = 3.76GLEE314 pKa = 3.91FMGHH318 pKa = 6.91DD319 pKa = 4.24FLDD322 pKa = 3.89TGPAPLYY329 pKa = 10.3LEE331 pKa = 4.08KK332 pKa = 10.76HH333 pKa = 5.85FSRR336 pKa = 11.84FLHH339 pKa = 6.73LEE341 pKa = 3.97EE342 pKa = 5.48EE343 pKa = 4.34IVPDD347 pKa = 5.35FIEE350 pKa = 4.56SMARR354 pKa = 11.84LYY356 pKa = 11.31VKK358 pKa = 9.63TPYY361 pKa = 9.62YY362 pKa = 9.42WLWEE366 pKa = 4.64HH367 pKa = 6.81ISVAMGCIVPSKK379 pKa = 10.12FALEE383 pKa = 3.58RR384 pKa = 11.84WYY386 pKa = 10.59DD387 pKa = 3.39HH388 pKa = 7.19SDD390 pKa = 2.9

Molecular weight: 45.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEI0|A0A1L3KEI0_9VIRU RNA-directed RNA polymerase OS=Hubei sobemo-like virus 45 OX=1923233 PE=4 SV=1
MM1 pKa = 7.83PFFEE5 pKa = 4.8EE6 pKa = 4.58LVCFGGHH13 pKa = 5.57QLWNQPTTVWGRR25 pKa = 11.84LFDD28 pKa = 3.84CSAILGGSPGPFRR41 pKa = 11.84VAAYY45 pKa = 9.93CGAAGALAAGAYY57 pKa = 10.02VGFPKK62 pKa = 10.46LVNSVRR68 pKa = 11.84RR69 pKa = 11.84SFGWRR74 pKa = 11.84PPMKK78 pKa = 9.33MDD80 pKa = 3.52VSPASVTNHH89 pKa = 4.74GRR91 pKa = 11.84LAEE94 pKa = 4.09SRR96 pKa = 11.84FAGSAEE102 pKa = 4.2TAMSMAVGQVAVGLYY117 pKa = 8.92MEE119 pKa = 4.9GSFRR123 pKa = 11.84VSGSGFRR130 pKa = 11.84VDD132 pKa = 4.53VGDD135 pKa = 4.64LEE137 pKa = 4.43TLIVPAHH144 pKa = 4.93VWEE147 pKa = 4.32EE148 pKa = 4.04VVARR152 pKa = 11.84SGDD155 pKa = 3.5RR156 pKa = 11.84VVVRR160 pKa = 11.84GKK162 pKa = 10.49KK163 pKa = 10.17ADD165 pKa = 3.61VEE167 pKa = 4.27LLDD170 pKa = 3.88SEE172 pKa = 4.58VRR174 pKa = 11.84KK175 pKa = 10.28HH176 pKa = 6.47EE177 pKa = 3.58IHH179 pKa = 6.46TDD181 pKa = 3.0VFLVVMPPNAFSKK194 pKa = 10.78AGVGRR199 pKa = 11.84STIVPIVGKK208 pKa = 10.49LSAKK212 pKa = 9.74VAGPHH217 pKa = 5.93GMGTFGTLSLGEE229 pKa = 3.91RR230 pKa = 11.84AAFGTTWYY238 pKa = 10.47HH239 pKa = 5.38GTTLPGYY246 pKa = 9.35SGSPYY251 pKa = 9.42TVSGNIAAMHH261 pKa = 5.87LRR263 pKa = 11.84GAEE266 pKa = 4.11VQHH269 pKa = 6.37GPNIGVSAQMLYY281 pKa = 8.97VTAKK285 pKa = 10.04HH286 pKa = 5.94VLNQRR291 pKa = 11.84LEE293 pKa = 4.13EE294 pKa = 4.17TYY296 pKa = 10.7EE297 pKa = 3.94WLTEE301 pKa = 3.88MAQRR305 pKa = 11.84KK306 pKa = 9.21ADD308 pKa = 3.51IEE310 pKa = 4.21YY311 pKa = 9.51DD312 pKa = 3.71QSWRR316 pKa = 11.84SQDD319 pKa = 3.29TVRR322 pKa = 11.84VKK324 pKa = 11.06VKK326 pKa = 10.28GQFAIVDD333 pKa = 3.69KK334 pKa = 11.17DD335 pKa = 3.78AMNSAYY341 pKa = 10.37GDD343 pKa = 3.44DD344 pKa = 3.14WHH346 pKa = 6.66NQVRR350 pKa = 11.84YY351 pKa = 9.7RR352 pKa = 11.84DD353 pKa = 3.66YY354 pKa = 11.61TEE356 pKa = 4.02DD357 pKa = 3.36SKK359 pKa = 11.67RR360 pKa = 11.84NRR362 pKa = 11.84HH363 pKa = 5.33SALTQEE369 pKa = 4.56SSGNAARR376 pKa = 11.84PGVLPSPVGPQGLVAYY392 pKa = 9.51AGPSCQNTGSVSCEE406 pKa = 3.99EE407 pKa = 4.32LCMGLTDD414 pKa = 5.27AEE416 pKa = 4.57LKK418 pKa = 10.52RR419 pKa = 11.84LSSVANRR426 pKa = 11.84YY427 pKa = 8.69YY428 pKa = 10.31SQRR431 pKa = 11.84LNALKK436 pKa = 8.97ITSGQGQTPTII447 pKa = 4.01

Molecular weight: 48.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

837

390

447

418.5

46.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.766 ± 1.426

1.434 ± 0.07

5.496 ± 1.13

5.735 ± 0.453

4.182 ± 0.635

9.08 ± 1.275

3.106 ± 0.324

3.106 ± 0.496

4.062 ± 0.371

8.244 ± 1.006

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.106 ± 0.152

2.27 ± 0.663

4.898 ± 0.018

4.182 ± 0.291

5.854 ± 0.315

7.407 ± 0.669

4.182 ± 1.085

8.722 ± 1.035

3.345 ± 1.196

3.823 ± 0.36
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski