Pseudomonas sp. PGPPP3

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; unclassified Pseudomonas

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3785 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257D7C3|A0A257D7C3_9PSED TIGR02449 family protein OS=Pseudomonas sp. PGPPP3 OX=2015555 GN=CFE49_01630 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84LTALTLASLALAPLSARR20 pKa = 11.84AIDD23 pKa = 4.87LNDD26 pKa = 3.34QFSLVITPTLISDD39 pKa = 3.52YY40 pKa = 9.38RR41 pKa = 11.84ASGISQTLGDD51 pKa = 4.33PAAQLNVMLSHH62 pKa = 7.59ASGLYY67 pKa = 10.09AGVWTSNVDD76 pKa = 4.31FGPEE80 pKa = 3.33DD81 pKa = 3.77DD82 pKa = 5.0ARR84 pKa = 11.84QEE86 pKa = 4.03LEE88 pKa = 4.23YY89 pKa = 10.57FAGYY93 pKa = 8.89YY94 pKa = 8.08WQISDD99 pKa = 5.77AISLDD104 pKa = 3.13AWYY107 pKa = 8.62TRR109 pKa = 11.84YY110 pKa = 10.56DD111 pKa = 3.4FSGASQYY118 pKa = 10.19NQNDD122 pKa = 3.39VQFTLDD128 pKa = 3.21AYY130 pKa = 10.61GVLLGAKK137 pKa = 9.49YY138 pKa = 8.67ATNVKK143 pKa = 10.29GPDD146 pKa = 3.53YY147 pKa = 10.89EE148 pKa = 5.19DD149 pKa = 5.33EE150 pKa = 4.47NGDD153 pKa = 3.78WQTGAQDD160 pKa = 3.7EE161 pKa = 4.61DD162 pKa = 3.97LASAFIGYY170 pKa = 7.65RR171 pKa = 11.84TLLPAEE177 pKa = 4.32IGFEE181 pKa = 3.77ARR183 pKa = 11.84YY184 pKa = 9.93EE185 pKa = 4.07YY186 pKa = 11.21VDD188 pKa = 3.69YY189 pKa = 10.99KK190 pKa = 11.53DD191 pKa = 3.72EE192 pKa = 4.29VFWNSDD198 pKa = 3.6FTSSRR203 pKa = 11.84SDD205 pKa = 3.38YY206 pKa = 10.96RR207 pKa = 11.84DD208 pKa = 2.99WEE210 pKa = 4.46VKK212 pKa = 8.59LTRR215 pKa = 11.84DD216 pKa = 3.52LLGVTWGLSYY226 pKa = 10.74IDD228 pKa = 3.81TDD230 pKa = 4.01LSKK233 pKa = 10.97SEE235 pKa = 4.57CVNFMGYY242 pKa = 10.77DD243 pKa = 3.85DD244 pKa = 4.52VCSATLVASASKK256 pKa = 9.63TFF258 pKa = 3.45

Molecular weight: 28.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257D260|A0A257D260_9PSED CDP-6-deoxy-delta-3 4-glucoseen reductase OS=Pseudomonas sp. PGPPP3 OX=2015555 GN=CFE49_11550 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3785

0

3785

1019170

23

2240

269.3

29.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.613 ± 0.053

1.066 ± 0.014

5.209 ± 0.03

5.672 ± 0.038

3.517 ± 0.026

7.74 ± 0.044

2.273 ± 0.023

4.502 ± 0.032

3.27 ± 0.033

12.588 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.188 ± 0.022

2.959 ± 0.027

4.815 ± 0.031

5.157 ± 0.04

6.367 ± 0.043

5.867 ± 0.033

4.462 ± 0.036

6.802 ± 0.037

1.439 ± 0.019

2.492 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski