Chilli leaf curl Gonda virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088MQ09|A0A088MQ09_9GEMI AC4 protein OS=Chilli leaf curl Gonda virus OX=2560380 GN=AC4 PE=3 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84DD3 pKa = 3.34PLLNHH8 pKa = 6.96LPEE11 pKa = 4.59TVHH14 pKa = 7.04GLTCLLPVLYY24 pKa = 9.92LYY26 pKa = 9.9TVEE29 pKa = 4.42ITLSPDD35 pKa = 2.96TLKK38 pKa = 11.01YY39 pKa = 10.96DD40 pKa = 5.19LIRR43 pKa = 11.84DD44 pKa = 4.66LISVIRR50 pKa = 11.84ARR52 pKa = 11.84KK53 pKa = 8.52YY54 pKa = 9.63VEE56 pKa = 3.44ATSRR60 pKa = 11.84YY61 pKa = 7.06NHH63 pKa = 5.67FHH65 pKa = 6.32SRR67 pKa = 11.84LEE69 pKa = 4.17GKK71 pKa = 8.84PQFQLCQPIYY81 pKa = 10.29QPCCCPHH88 pKa = 6.73CPRR91 pKa = 11.84HH92 pKa = 5.23QSKK95 pKa = 11.28SMGQQAHH102 pKa = 5.16EE103 pKa = 4.33QEE105 pKa = 4.51AQDD108 pKa = 3.72VQDD111 pKa = 3.72VQKK114 pKa = 10.11PRR116 pKa = 11.84CSEE119 pKa = 3.97GLL121 pKa = 3.49

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088MYS1|A0A088MYS1_9GEMI Replication enhancer OS=Chilli leaf curl Gonda virus OX=2560380 GN=AC3 PE=3 SV=1
MM1 pKa = 7.32GACLSKK7 pKa = 10.74RR8 pKa = 11.84SFSSKK13 pKa = 9.59GQHH16 pKa = 5.57SSEE19 pKa = 4.47TIDD22 pKa = 3.01ISMSLTLITPPISIRR37 pKa = 11.84TFRR40 pKa = 11.84EE41 pKa = 3.5LSRR44 pKa = 11.84VQMSKK49 pKa = 10.63PMWRR53 pKa = 11.84KK54 pKa = 7.78TEE56 pKa = 3.72TSLILVSFRR65 pKa = 11.84SMADD69 pKa = 3.07QLEE72 pKa = 4.53EE73 pKa = 4.17VANLPTTHH81 pKa = 6.76MPRR84 pKa = 11.84QSIQGRR90 pKa = 11.84LQRR93 pKa = 11.84PP94 pKa = 3.41

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1100

94

361

183.3

20.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.455 ± 0.946

2.273 ± 0.493

4.545 ± 0.6

4.727 ± 0.519

4.727 ± 0.586

4.818 ± 0.705

4.182 ± 0.76

5.545 ± 0.857

5.545 ± 0.727

7.364 ± 1.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.364 ± 0.6

5.091 ± 0.877

6.0 ± 0.446

4.818 ± 0.772

6.818 ± 0.781

8.273 ± 1.205

5.636 ± 0.888

6.091 ± 1.366

1.364 ± 0.215

3.364 ± 0.571
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski