Amino acid dipepetide frequency for Burkholderia phage BcepB1A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.426AlaAla: 15.426 ± 2.102
1.242AlaCys: 1.242 ± 0.288
6.275AlaAsp: 6.275 ± 0.865
4.706AlaGlu: 4.706 ± 0.516
4.379AlaPhe: 4.379 ± 0.575
9.543AlaGly: 9.543 ± 1.188
2.549AlaHis: 2.549 ± 0.449
6.406AlaIle: 6.406 ± 0.637
3.464AlaLys: 3.464 ± 0.544
8.955AlaLeu: 8.955 ± 0.786
4.314AlaMet: 4.314 ± 0.593
3.987AlaAsn: 3.987 ± 0.441
4.51AlaPro: 4.51 ± 0.769
5.36AlaGln: 5.36 ± 0.592
6.732AlaArg: 6.732 ± 1.034
6.079AlaSer: 6.079 ± 0.884
7.517AlaThr: 7.517 ± 0.923
6.406AlaVal: 6.406 ± 0.64
1.569AlaTrp: 1.569 ± 0.342
3.399AlaTyr: 3.399 ± 0.483
0.0AlaXaa: 0.0 ± 0.0
Cys
0.98CysAla: 0.98 ± 0.286
0.131CysCys: 0.131 ± 0.095
0.98CysAsp: 0.98 ± 0.284
0.784CysGlu: 0.784 ± 0.219
0.523CysPhe: 0.523 ± 0.206
0.784CysGly: 0.784 ± 0.259
0.196CysHis: 0.196 ± 0.105
0.392CysIle: 0.392 ± 0.178
0.588CysLys: 0.588 ± 0.227
0.588CysLeu: 0.588 ± 0.231
0.458CysMet: 0.458 ± 0.147
0.196CysAsn: 0.196 ± 0.149
0.523CysPro: 0.523 ± 0.192
0.261CysGln: 0.261 ± 0.132
0.784CysArg: 0.784 ± 0.2
0.588CysSer: 0.588 ± 0.179
0.85CysThr: 0.85 ± 0.218
0.458CysVal: 0.458 ± 0.196
0.131CysTrp: 0.131 ± 0.083
0.327CysTyr: 0.327 ± 0.147
0.0CysXaa: 0.0 ± 0.0
Asp
8.367AspAla: 8.367 ± 0.894
0.196AspCys: 0.196 ± 0.107
4.183AspAsp: 4.183 ± 0.569
4.249AspGlu: 4.249 ± 0.565
1.699AspPhe: 1.699 ± 0.291
6.798AspGly: 6.798 ± 0.718
1.373AspHis: 1.373 ± 0.274
3.399AspIle: 3.399 ± 0.491
3.007AspLys: 3.007 ± 0.547
3.726AspLeu: 3.726 ± 0.435
1.503AspMet: 1.503 ± 0.377
1.373AspAsn: 1.373 ± 0.329
3.072AspPro: 3.072 ± 0.496
1.896AspGln: 1.896 ± 0.411
3.072AspArg: 3.072 ± 0.445
2.941AspSer: 2.941 ± 0.406
3.726AspThr: 3.726 ± 0.604
4.641AspVal: 4.641 ± 0.424
0.719AspTrp: 0.719 ± 0.224
1.896AspTyr: 1.896 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
5.817GluAla: 5.817 ± 0.579
1.111GluCys: 1.111 ± 0.24
2.288GluAsp: 2.288 ± 0.483
2.484GluGlu: 2.484 ± 0.558
2.615GluPhe: 2.615 ± 0.404
3.791GluGly: 3.791 ± 0.541
1.111GluHis: 1.111 ± 0.26
2.418GluIle: 2.418 ± 0.476
2.288GluLys: 2.288 ± 0.395
4.51GluLeu: 4.51 ± 0.54
1.699GluMet: 1.699 ± 0.353
2.418GluAsn: 2.418 ± 0.377
1.177GluPro: 1.177 ± 0.264
3.007GluGln: 3.007 ± 0.472
4.118GluArg: 4.118 ± 0.667
2.745GluSer: 2.745 ± 0.431
3.137GluThr: 3.137 ± 0.461
2.615GluVal: 2.615 ± 0.372
1.242GluTrp: 1.242 ± 0.279
1.961GluTyr: 1.961 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
3.922PheAla: 3.922 ± 0.519
0.523PheCys: 0.523 ± 0.191
3.268PheAsp: 3.268 ± 0.469
3.334PheGlu: 3.334 ± 0.413
1.83PhePhe: 1.83 ± 0.442
3.66PheGly: 3.66 ± 0.548
0.458PheHis: 0.458 ± 0.245
2.418PheIle: 2.418 ± 0.451
1.634PheLys: 1.634 ± 0.257
2.353PheLeu: 2.353 ± 0.316
0.523PheMet: 0.523 ± 0.168
2.092PheAsn: 2.092 ± 0.277
1.765PhePro: 1.765 ± 0.341
1.046PheGln: 1.046 ± 0.235
1.569PheArg: 1.569 ± 0.282
1.765PheSer: 1.765 ± 0.36
2.418PheThr: 2.418 ± 0.373
2.549PheVal: 2.549 ± 0.346
0.588PheTrp: 0.588 ± 0.237
1.111PheTyr: 1.111 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
7.517GlyAla: 7.517 ± 0.962
0.654GlyCys: 0.654 ± 0.221
4.249GlyAsp: 4.249 ± 0.508
3.268GlyGlu: 3.268 ± 0.523
3.268GlyPhe: 3.268 ± 0.52
5.621GlyGly: 5.621 ± 0.807
1.242GlyHis: 1.242 ± 0.236
4.183GlyIle: 4.183 ± 0.418
4.379GlyLys: 4.379 ± 0.541
6.406GlyLeu: 6.406 ± 0.638
2.353GlyMet: 2.353 ± 0.728
2.418GlyAsn: 2.418 ± 0.364
2.222GlyPro: 2.222 ± 0.338
3.137GlyGln: 3.137 ± 0.414
4.641GlyArg: 4.641 ± 0.531
4.706GlySer: 4.706 ± 0.596
5.033GlyThr: 5.033 ± 1.018
6.471GlyVal: 6.471 ± 0.605
1.569GlyTrp: 1.569 ± 0.304
2.745GlyTyr: 2.745 ± 0.413
0.0GlyXaa: 0.0 ± 0.0
His
1.83HisAla: 1.83 ± 0.368
0.261HisCys: 0.261 ± 0.119
1.046HisAsp: 1.046 ± 0.256
1.177HisGlu: 1.177 ± 0.285
0.523HisPhe: 0.523 ± 0.166
1.83HisGly: 1.83 ± 0.389
0.327HisHis: 0.327 ± 0.146
1.503HisIle: 1.503 ± 0.344
0.588HisLys: 0.588 ± 0.177
1.503HisLeu: 1.503 ± 0.292
0.261HisMet: 0.261 ± 0.165
0.784HisAsn: 0.784 ± 0.284
0.784HisPro: 0.784 ± 0.205
0.98HisGln: 0.98 ± 0.366
1.438HisArg: 1.438 ± 0.287
1.438HisSer: 1.438 ± 0.259
1.177HisThr: 1.177 ± 0.291
1.634HisVal: 1.634 ± 0.415
0.523HisTrp: 0.523 ± 0.149
0.327HisTyr: 0.327 ± 0.134
0.0HisXaa: 0.0 ± 0.0
Ile
7.582IleAla: 7.582 ± 0.866
0.458IleCys: 0.458 ± 0.161
3.922IleAsp: 3.922 ± 0.576
4.118IleGlu: 4.118 ± 0.557
1.111IlePhe: 1.111 ± 0.218
4.118IleGly: 4.118 ± 0.508
1.373IleHis: 1.373 ± 0.339
2.418IleIle: 2.418 ± 0.341
2.288IleLys: 2.288 ± 0.33
2.549IleLeu: 2.549 ± 0.485
1.307IleMet: 1.307 ± 0.308
2.418IleAsn: 2.418 ± 0.409
3.072IlePro: 3.072 ± 0.468
1.699IleGln: 1.699 ± 0.303
3.399IleArg: 3.399 ± 0.45
3.334IleSer: 3.334 ± 0.561
3.007IleThr: 3.007 ± 0.433
3.726IleVal: 3.726 ± 0.535
0.654IleTrp: 0.654 ± 0.201
1.242IleTyr: 1.242 ± 0.223
0.0IleXaa: 0.0 ± 0.0
Lys
3.791LysAla: 3.791 ± 0.476
0.458LysCys: 0.458 ± 0.157
2.615LysAsp: 2.615 ± 0.422
1.699LysGlu: 1.699 ± 0.382
1.83LysPhe: 1.83 ± 0.272
2.615LysGly: 2.615 ± 0.408
1.111LysHis: 1.111 ± 0.251
1.896LysIle: 1.896 ± 0.328
3.072LysLys: 3.072 ± 0.49
3.66LysLeu: 3.66 ± 0.645
1.373LysMet: 1.373 ± 0.27
1.699LysAsn: 1.699 ± 0.268
3.203LysPro: 3.203 ± 0.636
2.288LysGln: 2.288 ± 0.295
3.53LysArg: 3.53 ± 0.448
1.765LysSer: 1.765 ± 0.42
3.268LysThr: 3.268 ± 0.432
2.745LysVal: 2.745 ± 0.438
0.784LysTrp: 0.784 ± 0.209
2.222LysTyr: 2.222 ± 0.373
0.0LysXaa: 0.0 ± 0.0
Leu
7.909LeuAla: 7.909 ± 0.702
0.523LeuCys: 0.523 ± 0.175
4.445LeuAsp: 4.445 ± 0.601
4.183LeuGlu: 4.183 ± 0.63
2.092LeuPhe: 2.092 ± 0.432
4.51LeuGly: 4.51 ± 0.403
1.634LeuHis: 1.634 ± 0.347
4.902LeuIle: 4.902 ± 0.588
3.53LeuLys: 3.53 ± 0.706
4.51LeuLeu: 4.51 ± 0.564
2.026LeuMet: 2.026 ± 0.382
3.203LeuAsn: 3.203 ± 0.439
3.791LeuPro: 3.791 ± 0.542
3.922LeuGln: 3.922 ± 0.479
5.425LeuArg: 5.425 ± 0.516
4.445LeuSer: 4.445 ± 0.536
5.294LeuThr: 5.294 ± 0.639
3.856LeuVal: 3.856 ± 0.564
0.784LeuTrp: 0.784 ± 0.234
1.569LeuTyr: 1.569 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
2.092MetAla: 2.092 ± 0.401
0.131MetCys: 0.131 ± 0.085
2.157MetAsp: 2.157 ± 0.458
0.915MetGlu: 0.915 ± 0.209
0.915MetPhe: 0.915 ± 0.255
2.092MetGly: 2.092 ± 0.45
0.523MetHis: 0.523 ± 0.177
1.503MetIle: 1.503 ± 0.288
1.111MetLys: 1.111 ± 0.251
2.092MetLeu: 2.092 ± 0.379
0.719MetMet: 0.719 ± 0.219
1.569MetAsn: 1.569 ± 0.345
1.765MetPro: 1.765 ± 0.304
1.699MetGln: 1.699 ± 0.464
2.484MetArg: 2.484 ± 0.496
1.765MetSer: 1.765 ± 0.327
2.549MetThr: 2.549 ± 0.442
1.438MetVal: 1.438 ± 0.241
0.458MetTrp: 0.458 ± 0.194
0.392MetTyr: 0.392 ± 0.146
0.0MetXaa: 0.0 ± 0.0
Asn
5.425AsnAla: 5.425 ± 0.733
0.654AsnCys: 0.654 ± 0.208
1.961AsnAsp: 1.961 ± 0.417
2.157AsnGlu: 2.157 ± 0.389
1.699AsnPhe: 1.699 ± 0.51
4.314AsnGly: 4.314 ± 0.707
0.588AsnHis: 0.588 ± 0.195
1.961AsnIle: 1.961 ± 0.4
1.634AsnLys: 1.634 ± 0.302
2.418AsnLeu: 2.418 ± 0.37
1.046AsnMet: 1.046 ± 0.219
2.353AsnAsn: 2.353 ± 0.62
2.68AsnPro: 2.68 ± 0.388
1.438AsnGln: 1.438 ± 0.327
1.373AsnArg: 1.373 ± 0.295
2.026AsnSer: 2.026 ± 0.365
1.896AsnThr: 1.896 ± 0.316
2.484AsnVal: 2.484 ± 0.407
0.523AsnTrp: 0.523 ± 0.146
1.242AsnTyr: 1.242 ± 0.237
0.0AsnXaa: 0.0 ± 0.0
Pro
5.164ProAla: 5.164 ± 0.734
0.588ProCys: 0.588 ± 0.203
4.053ProAsp: 4.053 ± 0.536
3.072ProGlu: 3.072 ± 0.438
1.961ProPhe: 1.961 ± 0.355
2.876ProGly: 2.876 ± 0.554
1.307ProHis: 1.307 ± 0.25
2.615ProIle: 2.615 ± 0.506
2.288ProLys: 2.288 ± 0.384
3.53ProLeu: 3.53 ± 0.565
1.373ProMet: 1.373 ± 0.285
2.941ProAsn: 2.941 ± 0.477
1.503ProPro: 1.503 ± 0.312
2.092ProGln: 2.092 ± 0.373
1.765ProArg: 1.765 ± 0.282
2.745ProSer: 2.745 ± 0.42
2.745ProThr: 2.745 ± 0.502
4.118ProVal: 4.118 ± 0.582
0.588ProTrp: 0.588 ± 0.164
0.98ProTyr: 0.98 ± 0.216
0.0ProXaa: 0.0 ± 0.0
Gln
4.706GlnAla: 4.706 ± 0.709
0.458GlnCys: 0.458 ± 0.194
1.896GlnAsp: 1.896 ± 0.286
1.438GlnGlu: 1.438 ± 0.239
2.353GlnPhe: 2.353 ± 0.345
3.203GlnGly: 3.203 ± 0.654
0.85GlnHis: 0.85 ± 0.249
2.092GlnIle: 2.092 ± 0.344
1.634GlnLys: 1.634 ± 0.307
4.183GlnLeu: 4.183 ± 0.57
1.438GlnMet: 1.438 ± 0.37
1.699GlnAsn: 1.699 ± 0.345
2.484GlnPro: 2.484 ± 0.447
2.876GlnGln: 2.876 ± 0.476
3.268GlnArg: 3.268 ± 0.527
2.615GlnSer: 2.615 ± 0.44
2.353GlnThr: 2.353 ± 0.449
2.549GlnVal: 2.549 ± 0.466
1.111GlnTrp: 1.111 ± 0.324
1.242GlnTyr: 1.242 ± 0.296
0.0GlnXaa: 0.0 ± 0.0
Arg
7.778ArgAla: 7.778 ± 0.954
1.111ArgCys: 1.111 ± 0.262
4.314ArgAsp: 4.314 ± 0.567
3.53ArgGlu: 3.53 ± 0.628
2.484ArgPhe: 2.484 ± 0.393
3.399ArgGly: 3.399 ± 0.672
1.307ArgHis: 1.307 ± 0.332
4.053ArgIle: 4.053 ± 0.546
3.791ArgLys: 3.791 ± 0.517
4.314ArgLeu: 4.314 ± 0.641
2.222ArgMet: 2.222 ± 0.457
2.026ArgAsn: 2.026 ± 0.297
2.418ArgPro: 2.418 ± 0.413
1.699ArgGln: 1.699 ± 0.359
2.745ArgArg: 2.745 ± 0.529
3.137ArgSer: 3.137 ± 0.501
2.941ArgThr: 2.941 ± 0.406
4.445ArgVal: 4.445 ± 0.473
0.719ArgTrp: 0.719 ± 0.259
1.503ArgTyr: 1.503 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
5.36SerAla: 5.36 ± 0.657
0.065SerCys: 0.065 ± 0.061
4.118SerAsp: 4.118 ± 0.408
3.072SerGlu: 3.072 ± 0.538
2.222SerPhe: 2.222 ± 0.517
4.183SerGly: 4.183 ± 0.592
1.438SerHis: 1.438 ± 0.325
3.464SerIle: 3.464 ± 0.414
2.353SerLys: 2.353 ± 0.429
3.399SerLeu: 3.399 ± 0.504
1.307SerMet: 1.307 ± 0.305
2.68SerAsn: 2.68 ± 0.392
2.876SerPro: 2.876 ± 0.511
2.026SerGln: 2.026 ± 0.361
2.745SerArg: 2.745 ± 0.483
3.464SerSer: 3.464 ± 0.676
4.314SerThr: 4.314 ± 0.608
3.726SerVal: 3.726 ± 0.53
1.111SerTrp: 1.111 ± 0.241
1.699SerTyr: 1.699 ± 0.364
0.0SerXaa: 0.0 ± 0.0
Thr
6.013ThrAla: 6.013 ± 0.718
0.327ThrCys: 0.327 ± 0.13
3.203ThrAsp: 3.203 ± 0.412
2.876ThrGlu: 2.876 ± 0.38
3.072ThrPhe: 3.072 ± 0.521
5.098ThrGly: 5.098 ± 0.61
0.98ThrHis: 0.98 ± 0.211
3.334ThrIle: 3.334 ± 0.495
2.484ThrLys: 2.484 ± 0.444
6.21ThrLeu: 6.21 ± 0.607
1.634ThrMet: 1.634 ± 0.331
2.288ThrAsn: 2.288 ± 0.386
4.837ThrPro: 4.837 ± 0.6
2.222ThrGln: 2.222 ± 0.421
2.941ThrArg: 2.941 ± 0.52
3.203ThrSer: 3.203 ± 0.541
4.053ThrThr: 4.053 ± 0.616
5.556ThrVal: 5.556 ± 0.566
1.111ThrTrp: 1.111 ± 0.306
2.68ThrTyr: 2.68 ± 0.327
0.0ThrXaa: 0.0 ± 0.0
Val
8.17ValAla: 8.17 ± 0.899
0.85ValCys: 0.85 ± 0.254
4.51ValAsp: 4.51 ± 0.693
3.464ValGlu: 3.464 ± 0.418
2.157ValPhe: 2.157 ± 0.439
4.183ValGly: 4.183 ± 0.511
0.588ValHis: 0.588 ± 0.212
3.007ValIle: 3.007 ± 0.421
3.399ValLys: 3.399 ± 0.435
4.249ValLeu: 4.249 ± 0.451
1.634ValMet: 1.634 ± 0.377
1.83ValAsn: 1.83 ± 0.348
3.595ValPro: 3.595 ± 0.455
4.053ValGln: 4.053 ± 0.52
4.641ValArg: 4.641 ± 0.571
4.706ValSer: 4.706 ± 0.548
4.902ValThr: 4.902 ± 0.553
5.294ValVal: 5.294 ± 0.605
1.111ValTrp: 1.111 ± 0.325
2.026ValTyr: 2.026 ± 0.419
0.0ValXaa: 0.0 ± 0.0
Trp
1.111TrpAla: 1.111 ± 0.301
0.523TrpCys: 0.523 ± 0.218
0.719TrpAsp: 0.719 ± 0.166
0.784TrpGlu: 0.784 ± 0.21
1.046TrpPhe: 1.046 ± 0.245
1.177TrpGly: 1.177 ± 0.29
0.523TrpHis: 0.523 ± 0.183
0.784TrpIle: 0.784 ± 0.229
0.588TrpLys: 0.588 ± 0.196
1.569TrpLeu: 1.569 ± 0.27
0.392TrpMet: 0.392 ± 0.158
0.588TrpAsn: 0.588 ± 0.171
0.392TrpPro: 0.392 ± 0.165
1.046TrpGln: 1.046 ± 0.286
1.177TrpArg: 1.177 ± 0.294
0.719TrpSer: 0.719 ± 0.182
1.242TrpThr: 1.242 ± 0.243
0.915TrpVal: 0.915 ± 0.229
0.131TrpTrp: 0.131 ± 0.088
0.85TrpTyr: 0.85 ± 0.211
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.856TyrAla: 3.856 ± 0.5
0.261TyrCys: 0.261 ± 0.124
1.699TyrAsp: 1.699 ± 0.342
1.242TyrGlu: 1.242 ± 0.327
1.046TyrPhe: 1.046 ± 0.246
2.288TyrGly: 2.288 ± 0.387
0.458TyrHis: 0.458 ± 0.192
1.046TyrIle: 1.046 ± 0.233
1.503TyrLys: 1.503 ± 0.318
1.83TyrLeu: 1.83 ± 0.418
0.719TyrMet: 0.719 ± 0.219
1.307TyrAsn: 1.307 ± 0.208
1.634TyrPro: 1.634 ± 0.301
1.699TyrGln: 1.699 ± 0.285
2.092TyrArg: 2.092 ± 0.396
1.438TyrSer: 1.438 ± 0.336
1.699TyrThr: 1.699 ± 0.429
2.745TyrVal: 2.745 ± 0.397
0.85TyrTrp: 0.85 ± 0.196
0.719TyrTyr: 0.719 ± 0.232
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 73 proteins (15300 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski