Azospirillum sp. CAG:260

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Azospirillaceae; Azospirillum; environmental samples

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6HQV3|R6HQV3_9PROT Shikimate kinase OS=Azospirillum sp. CAG:260 OX=1262706 GN=aroK PE=3 SV=1
MM1 pKa = 7.41TSYY4 pKa = 10.89ILQVHH9 pKa = 5.71SRR11 pKa = 11.84YY12 pKa = 10.05LDD14 pKa = 3.73LPLVPPSTGIARR26 pKa = 11.84HH27 pKa = 5.34TGYY30 pKa = 11.17DD31 pKa = 3.99LVTLNDD37 pKa = 3.4NGEE40 pKa = 3.92PSFINSVDD48 pKa = 3.16LTTRR52 pKa = 11.84NGRR55 pKa = 11.84IYY57 pKa = 9.79MVFVEE62 pKa = 5.39GSDD65 pKa = 3.53GQPFIVNVSGEE76 pKa = 4.03KK77 pKa = 10.34QIWPKK82 pKa = 10.11KK83 pKa = 7.88IHH85 pKa = 6.3IGEE88 pKa = 4.17VTADD92 pKa = 3.37NEE94 pKa = 4.72GPNFTVTQEE103 pKa = 3.99GAEE106 pKa = 4.12KK107 pKa = 10.88YY108 pKa = 10.07DD109 pKa = 3.68FKK111 pKa = 10.91TATVLTGDD119 pKa = 3.78VAKK122 pKa = 10.58HH123 pKa = 4.57VFDD126 pKa = 5.54WIVFDD131 pKa = 4.18AKK133 pKa = 10.76YY134 pKa = 10.72RR135 pKa = 11.84NALSMVSNGLTDD147 pKa = 3.87YY148 pKa = 11.25DD149 pKa = 4.41VNAPNCNTWTNYY161 pKa = 9.65IDD163 pKa = 3.33LTYY166 pKa = 10.62IKK168 pKa = 10.23GSQSVFDD175 pKa = 4.12KK176 pKa = 11.35LGGGWYY182 pKa = 9.23PGKK185 pKa = 10.39DD186 pKa = 2.82KK187 pKa = 11.07TFINSTSGDD196 pKa = 3.75DD197 pKa = 5.23LRR199 pKa = 11.84DD200 pKa = 3.54CKK202 pKa = 10.57IIEE205 pKa = 4.84DD206 pKa = 3.54ISRR209 pKa = 11.84KK210 pKa = 9.11FFEE213 pKa = 5.58LYY215 pKa = 10.48SADD218 pKa = 3.72FNFGNLSITLEE229 pKa = 4.21DD230 pKa = 3.26VKK232 pKa = 10.92HH233 pKa = 5.9YY234 pKa = 10.73SNTMDD239 pKa = 3.39PVGFVTRR246 pKa = 11.84ISDD249 pKa = 3.73GVRR252 pKa = 11.84SYY254 pKa = 11.62LFDD257 pKa = 4.45CEE259 pKa = 4.5VVNSYY264 pKa = 10.3VQDD267 pKa = 3.12GHH269 pKa = 6.67YY270 pKa = 10.85FEE272 pKa = 6.7DD273 pKa = 3.81NTPGGSYY280 pKa = 9.83IVAGKK285 pKa = 9.69GASEE289 pKa = 4.19TIIAGDD295 pKa = 3.71GDD297 pKa = 4.77DD298 pKa = 5.54IIDD301 pKa = 3.83AGAGEE306 pKa = 5.06GEE308 pKa = 4.34TAVAKK313 pKa = 9.95YY314 pKa = 10.71VNLGGGDD321 pKa = 3.51NLYY324 pKa = 11.05VGGAAADD331 pKa = 3.92EE332 pKa = 4.85VNTGTDD338 pKa = 2.96NSGSVANEE346 pKa = 3.34NNVNHH351 pKa = 5.89VFLGAGSDD359 pKa = 3.34TFYY362 pKa = 10.94GGKK365 pKa = 10.09GADD368 pKa = 3.35IVDD371 pKa = 4.26GGSGDD376 pKa = 3.93LTRR379 pKa = 11.84LQALPQFIDD388 pKa = 3.32SGVTTEE394 pKa = 5.96SMADD398 pKa = 3.57DD399 pKa = 3.91EE400 pKa = 4.76NAVNRR405 pKa = 11.84INLGVGNDD413 pKa = 3.42VYY415 pKa = 11.18FGGKK419 pKa = 9.92GKK421 pKa = 10.27DD422 pKa = 3.09AVYY425 pKa = 10.56GGDD428 pKa = 4.03GDD430 pKa = 4.96DD431 pKa = 4.19IIYY434 pKa = 10.85GGDD437 pKa = 3.56CPGGFDD443 pKa = 5.46GRR445 pKa = 11.84NEE447 pKa = 4.27LYY449 pKa = 11.03GEE451 pKa = 4.54AGDD454 pKa = 5.93DD455 pKa = 4.13YY456 pKa = 11.24ICGGRR461 pKa = 11.84SDD463 pKa = 5.14DD464 pKa = 4.21VISGGEE470 pKa = 4.21GNDD473 pKa = 3.3VIYY476 pKa = 10.88GNGGNDD482 pKa = 3.25VLMADD487 pKa = 4.06AGSDD491 pKa = 3.27IIYY494 pKa = 10.71AGVGYY499 pKa = 10.85NNIYY503 pKa = 10.55CGEE506 pKa = 4.06NDD508 pKa = 3.37SCRR511 pKa = 11.84DD512 pKa = 3.31IVVLNNDD519 pKa = 2.86VNGRR523 pKa = 11.84DD524 pKa = 3.87DD525 pKa = 3.72IYY527 pKa = 11.2NITSQDD533 pKa = 3.47IICCVGGFDD542 pKa = 4.55FSRR545 pKa = 11.84SLGYY549 pKa = 9.98YY550 pKa = 9.45SPLWGVKK557 pKa = 9.76GNSINFQTTGDD568 pKa = 4.22NVPLIYY574 pKa = 10.81DD575 pKa = 3.69ADD577 pKa = 3.7GKK579 pKa = 9.3VYY581 pKa = 10.17RR582 pKa = 11.84WRR584 pKa = 11.84EE585 pKa = 3.65ACTDD589 pKa = 3.43ADD591 pKa = 4.84GITHH595 pKa = 6.77SSQYY599 pKa = 9.56IDD601 pKa = 3.03TGIRR605 pKa = 11.84YY606 pKa = 7.45PAYY609 pKa = 9.98GDD611 pKa = 3.94GEE613 pKa = 4.3PEE615 pKa = 3.93EE616 pKa = 4.97EE617 pKa = 4.44DD618 pKa = 3.9LKK620 pKa = 11.51KK621 pKa = 10.86LLSAA625 pKa = 5.11

Molecular weight: 67.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6I3Z9|R6I3Z9_9PROT Ribonuclease H OS=Azospirillum sp. CAG:260 OX=1262706 GN=rnhA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATAGGRR28 pKa = 11.84KK29 pKa = 8.92VLAARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.95HH37 pKa = 5.78GRR39 pKa = 11.84AKK41 pKa = 10.77LSAA44 pKa = 3.95

Molecular weight: 5.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1762

0

1762

526901

29

2605

299.0

33.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.57 ± 0.065

1.235 ± 0.03

5.644 ± 0.042

6.815 ± 0.071

4.326 ± 0.047

6.668 ± 0.077

1.627 ± 0.025

6.755 ± 0.055

7.01 ± 0.067

9.506 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.026

4.981 ± 0.055

3.869 ± 0.042

3.486 ± 0.041

4.987 ± 0.048

5.842 ± 0.051

4.881 ± 0.047

6.55 ± 0.056

1.056 ± 0.022

3.65 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski