Rhodoferax saidenbachensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Rhodoferax

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3921 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8K6Z5|A0A1P8K6Z5_9BURK Uncharacterized protein OS=Rhodoferax saidenbachensis OX=1484693 GN=RS694_03970 PE=4 SV=1
MM1 pKa = 7.25SAVAEE6 pKa = 4.41NIQTEE11 pKa = 4.54MPAPILFTDD20 pKa = 3.95SAAAKK25 pKa = 10.02VADD28 pKa = 5.45LIAEE32 pKa = 4.31EE33 pKa = 4.82GNPDD37 pKa = 2.99LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.94GFTFDD59 pKa = 4.81EE60 pKa = 4.64ITNEE64 pKa = 4.77DD65 pKa = 4.2DD66 pKa = 3.25TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 9.99QYY85 pKa = 11.86LMGAEE90 pKa = 3.9IDD92 pKa = 4.02YY93 pKa = 11.33KK94 pKa = 11.12EE95 pKa = 4.67DD96 pKa = 3.23LQGAQFVIKK105 pKa = 10.43NPNATSTCGCGSSFSTT121 pKa = 4.55

Molecular weight: 12.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8K9L3|A0A1P8K9L3_9BURK Cobalamin-binding protein OS=Rhodoferax saidenbachensis OX=1484693 GN=RS694_09155 PE=4 SV=1
MM1 pKa = 7.91PKK3 pKa = 9.67MKK5 pKa = 9.88TKK7 pKa = 10.49SAAKK11 pKa = 9.4KK12 pKa = 9.52RR13 pKa = 11.84FRR15 pKa = 11.84VRR17 pKa = 11.84PGGTVKK23 pKa = 10.62RR24 pKa = 11.84GQAFKK29 pKa = 11.01RR30 pKa = 11.84HH31 pKa = 5.79ILTKK35 pKa = 9.78KK36 pKa = 3.94TTKK39 pKa = 10.22NKK41 pKa = 8.75RR42 pKa = 11.84HH43 pKa = 5.84LRR45 pKa = 11.84GAVTVHH51 pKa = 5.61PTNMGHH57 pKa = 6.22MAQMLPGRR65 pKa = 11.84GII67 pKa = 3.49

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3921

0

3921

1271949

32

2075

324.4

35.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.408 ± 0.047

0.907 ± 0.011

5.101 ± 0.03

5.097 ± 0.035

3.584 ± 0.023

7.963 ± 0.036

2.218 ± 0.018

4.512 ± 0.028

3.861 ± 0.038

10.817 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.661 ± 0.018

2.859 ± 0.021

4.914 ± 0.028

4.408 ± 0.03

5.958 ± 0.032

5.583 ± 0.027

5.597 ± 0.035

7.834 ± 0.032

1.472 ± 0.02

2.246 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski