Solemya pervernicosa gill symbiont
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3236 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1T2L373|A0A1T2L373_9GAMM Probable chromosome-partitioning protein ParB OS=Solemya pervernicosa gill symbiont OX=642797 GN=BOW53_11815 PE=3 SV=1
MM1 pKa = 6.96 STNEE5 pKa = 4.0 APSASFIVDD14 pKa = 3.39 VGTGHH19 pKa = 6.61 SPLMVNFDD27 pKa = 4.56 ASPSSDD33 pKa = 3.07 SDD35 pKa = 3.71 GSIAGYY41 pKa = 9.92 SWLFGDD47 pKa = 5.12 GSTATGQQVSHH58 pKa = 6.98 SYY60 pKa = 10.47 TNVGNHH66 pKa = 5.5 SVTLTVTDD74 pKa = 3.57 NEE76 pKa = 4.4 GAIDD80 pKa = 3.86 NATPQTISVWIANNAPTALFSVDD103 pKa = 3.28 TDD105 pKa = 3.64 SGEE108 pKa = 4.36 GPLTVNFDD116 pKa = 3.52 ASASGDD122 pKa = 3.45 SDD124 pKa = 3.75 GTIVAYY130 pKa = 9.89 GWDD133 pKa = 3.87 FGDD136 pKa = 3.96 GSSGNGQTVAHH147 pKa = 7.09 SYY149 pKa = 6.39 TTPGVYY155 pKa = 10.2 LATLTVRR162 pKa = 11.84 DD163 pKa = 4.28 DD164 pKa = 3.91 DD165 pKa = 4.52 AATKK169 pKa = 10.65 LSTAHH174 pKa = 7.71 IIRR177 pKa = 11.84 VLPANQAPTAAFSVDD192 pKa = 3.45 TDD194 pKa = 3.51 SGTTPLTVNFDD205 pKa = 3.69 ASGSTDD211 pKa = 3.11 TDD213 pKa = 3.8 GSIVSYY219 pKa = 10.76 AWDD222 pKa = 4.21 FGDD225 pKa = 4.03 GDD227 pKa = 4.16 SGVGQQVSHH236 pKa = 7.16 TYY238 pKa = 10.08 TSDD241 pKa = 2.97 GSYY244 pKa = 9.36 TATLTVTDD252 pKa = 3.73 NAGAEE257 pKa = 4.27 DD258 pKa = 4.24 SDD260 pKa = 4.47 SDD262 pKa = 4.45 VIDD265 pKa = 4.76 SISASHH271 pKa = 6.49 YY272 pKa = 6.45 PTARR276 pKa = 11.84 YY277 pKa = 9.51 DD278 pKa = 3.41 VDD280 pKa = 3.9 VDD282 pKa = 3.97 SGPAPLTVSFDD293 pKa = 3.6 ASTSSDD299 pKa = 3.65 PDD301 pKa = 3.24 NDD303 pKa = 3.71 IINYY307 pKa = 8.04 FWQFGDD313 pKa = 3.55 GDD315 pKa = 3.98 NASGVTTSHH324 pKa = 7.15 TYY326 pKa = 8.4 TVPGSYY332 pKa = 10.0 DD333 pKa = 3.49 VQMLATDD340 pKa = 3.86 SASQGDD346 pKa = 4.08 VADD349 pKa = 3.55 VRR351 pKa = 11.84 TITVQGPNTLPTAAFTVDD369 pKa = 3.77 VNSGTSPVTVNFDD382 pKa = 3.2 ATTSSDD388 pKa = 2.96 SDD390 pKa = 3.96 GSVVGYY396 pKa = 10.19 GWDD399 pKa = 3.7 FGDD402 pKa = 4.42 GFQGSGASTTHH413 pKa = 6.62 TYY415 pKa = 10.2 FADD418 pKa = 4.17 GTHH421 pKa = 5.75 TAILTVTDD429 pKa = 3.95 DD430 pKa = 3.53 RR431 pKa = 11.84 GGQHH435 pKa = 6.09 SVSHH439 pKa = 7.35 DD440 pKa = 3.09 ISVAASGGVTLSGVLGLSGTGTVDD464 pKa = 4.25 SDD466 pKa = 4.22 VNDD469 pKa = 3.41 INADD473 pKa = 3.66 YY474 pKa = 10.94 QSNNTTATAQLLISPSSLGNVWGFVSKK501 pKa = 9.19 TATGIEE507 pKa = 4.44 GEE509 pKa = 4.27 RR510 pKa = 11.84 FTYY513 pKa = 10.52 SADD516 pKa = 3.32 EE517 pKa = 3.94 VDD519 pKa = 3.91 IYY521 pKa = 10.88 RR522 pKa = 11.84 AYY524 pKa = 10.93 LPVGQRR530 pKa = 11.84 ICLTIKK536 pKa = 10.87 NPDD539 pKa = 3.79 DD540 pKa = 3.54 EE541 pKa = 5.02 TGLEE545 pKa = 4.08 NANDD549 pKa = 4.13 LDD551 pKa = 4.49 LYY553 pKa = 10.61 LYY555 pKa = 10.91 SVDD558 pKa = 4.88 DD559 pKa = 4.23 PEE561 pKa = 5.91 SPVATALGTHH571 pKa = 5.89 SSEE574 pKa = 4.3 TLHH577 pKa = 6.4 VPTGGEE583 pKa = 4.07 YY584 pKa = 10.19 YY585 pKa = 10.08 IVVAAYY591 pKa = 9.97 RR592 pKa = 11.84 GKK594 pKa = 8.98 STYY597 pKa = 10.95 KK598 pKa = 10.46 LATFMDD604 pKa = 4.55 LTSVDD609 pKa = 3.6 EE610 pKa = 4.45 VQTLRR615 pKa = 11.84 LEE617 pKa = 4.53 DD618 pKa = 4.31 DD619 pKa = 4.44 FVPGQVIAKK628 pKa = 9.53 LKK630 pKa = 10.54 SEE632 pKa = 4.3 VVNPPGAGADD642 pKa = 3.58 KK643 pKa = 10.71 KK644 pKa = 11.11 GVAAATAIEE653 pKa = 4.57 SVSSRR658 pKa = 11.84 GSVSMIRR665 pKa = 11.84 GAADD669 pKa = 3.64 RR670 pKa = 11.84 ASLFSVNDD678 pKa = 3.3 MGQNSASGKK687 pKa = 8.53 LQSAASSDD695 pKa = 3.65 QDD697 pKa = 3.25 EE698 pKa = 4.85 SEE700 pKa = 4.15 YY701 pKa = 11.53 SLLDD705 pKa = 3.11 GRR707 pKa = 11.84 YY708 pKa = 10.32 GIGLSQKK715 pKa = 10.26 DD716 pKa = 3.26 RR717 pKa = 11.84 EE718 pKa = 4.34 AFEE721 pKa = 4.3 TIKK724 pKa = 10.69 AIKK727 pKa = 10.03 RR728 pKa = 11.84 LRR730 pKa = 11.84 QDD732 pKa = 2.64 SRR734 pKa = 11.84 IDD736 pKa = 3.56 YY737 pKa = 10.83 AEE739 pKa = 4.12 PNYY742 pKa = 8.52 IQKK745 pKa = 9.74 MSLVPDD751 pKa = 4.34 DD752 pKa = 4.45 LHH754 pKa = 8.86 YY755 pKa = 10.17 EE756 pKa = 4.35 QQWHH760 pKa = 5.72 YY761 pKa = 10.35 PAINLPDD768 pKa = 3.17 TWDD771 pKa = 3.26 ITTGDD776 pKa = 3.37 ASVIVAVIDD785 pKa = 3.52 SGVFMAHH792 pKa = 6.89 EE793 pKa = 4.67 DD794 pKa = 3.45 LSGNLLSTGYY804 pKa = 10.77 DD805 pKa = 3.88 FIVSTSMSNDD815 pKa = 2.87 GDD817 pKa = 4.88 GIDD820 pKa = 5.14 PDD822 pKa = 5.2 PDD824 pKa = 4.6 PDD826 pKa = 4.13 DD827 pKa = 5.11 PGDD830 pKa = 3.6 IVISYY835 pKa = 7.69 SVWHH839 pKa = 5.97 GTHH842 pKa = 5.28 VAGTIAASTNTTTGVAGVAPDD863 pKa = 3.71 VRR865 pKa = 11.84 IMPIRR870 pKa = 11.84 VLGEE874 pKa = 4.16 GGSGASYY881 pKa = 11.12 DD882 pKa = 3.72 VAQGILYY889 pKa = 10.41 AAGLANDD896 pKa = 4.16 SATVPPQAADD906 pKa = 3.78 IINLSLGGAYY916 pKa = 10.11 VNQASKK922 pKa = 11.11 DD923 pKa = 3.86 AISAALNAGVIVIAAKK939 pKa = 10.91 GNDD942 pKa = 3.93 GSDD945 pKa = 3.41 APHH948 pKa = 6.48 YY949 pKa = 9.75 PSDD952 pKa = 3.87 YY953 pKa = 10.22 EE954 pKa = 4.37 GVVSVGALGMGDD966 pKa = 3.35 EE967 pKa = 4.1 QAYY970 pKa = 8.77 YY971 pKa = 11.0 SNYY974 pKa = 9.9 GPTLDD979 pKa = 4.62 LVAPGGDD986 pKa = 3.38 SKK988 pKa = 11.19 IDD990 pKa = 3.53 ADD992 pKa = 4.02 NDD994 pKa = 3.7 GKK996 pKa = 10.82 FDD998 pKa = 3.74 STILSTSVDD1007 pKa = 3.38 VSSGSRR1013 pKa = 11.84 EE1014 pKa = 3.56 SGYY1017 pKa = 9.42 DD1018 pKa = 3.35 HH1019 pKa = 7.3 KK1020 pKa = 11.07 QGTSMATPHH1029 pKa = 5.58 VAGVIALMKK1038 pKa = 10.16 SVYY1041 pKa = 10.39 SEE1043 pKa = 4.07 LTPTLLTALIEE1054 pKa = 4.43 NGAITTDD1061 pKa = 3.73 LSGDD1065 pKa = 3.5 GAAVRR1070 pKa = 11.84 NDD1072 pKa = 2.98 TYY1074 pKa = 11.53 GYY1076 pKa = 11.1 GKK1078 pKa = 9.61 IDD1080 pKa = 3.47 ALKK1083 pKa = 10.55 AVQQAQAIAGGATPPVHH1100 pKa = 7.3 DD1101 pKa = 4.48 EE1102 pKa = 3.69 VGIIYY1107 pKa = 10.07 EE1108 pKa = 4.08 IDD1110 pKa = 3.51 NMVLSSSQTISIVKK1124 pKa = 9.22 WSGTFTVDD1132 pKa = 5.15 SITLNYY1138 pKa = 9.16 PWATLTPTAVDD1149 pKa = 3.67 GEE1151 pKa = 4.75 GFGDD1155 pKa = 3.82 YY1156 pKa = 10.66 QLDD1159 pKa = 3.31 IDD1161 pKa = 4.72 RR1162 pKa = 11.84 DD1163 pKa = 3.76 QFGDD1167 pKa = 3.67 GLYY1170 pKa = 10.98 LLDD1173 pKa = 3.53 ITFVMSNGDD1182 pKa = 3.41 QYY1184 pKa = 9.86 VTRR1187 pKa = 11.84 INVTDD1192 pKa = 3.71 RR1193 pKa = 11.84 VSTLDD1198 pKa = 3.39 EE1199 pKa = 4.34 RR1200 pKa = 11.84 DD1201 pKa = 3.28 VGNVYY1206 pKa = 9.59 MVLVDD1211 pKa = 4.29 EE1212 pKa = 4.6 STLNVVKK1219 pKa = 10.26 QIRR1222 pKa = 11.84 MNLDD1226 pKa = 2.83 VALFEE1231 pKa = 4.67 PEE1233 pKa = 4.52 VPFSMSNVPDD1243 pKa = 3.74 GTYY1246 pKa = 11.05 LLYY1249 pKa = 10.88 AGTDD1253 pKa = 3.75 FDD1255 pKa = 5.3 GDD1257 pKa = 3.62 RR1258 pKa = 11.84 LLEE1261 pKa = 4.48 DD1262 pKa = 4.1 DD1263 pKa = 4.8 EE1264 pKa = 4.89 YY1265 pKa = 11.1 FGRR1268 pKa = 11.84 GFYY1271 pKa = 11.04 DD1272 pKa = 3.33 HH1273 pKa = 8.0 DD1274 pKa = 4.88 DD1275 pKa = 4.96 DD1276 pKa = 6.06 IFTSDD1281 pKa = 3.08 ISPIVVNSADD1291 pKa = 3.45 IDD1293 pKa = 4.11 SVSIYY1298 pKa = 10.68 VGSTSADD1305 pKa = 3.28 PVGLIDD1311 pKa = 4.31
Molecular weight: 137.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.667
IPC2_protein 3.745
IPC_protein 3.821
Toseland 3.567
ProMoST 3.986
Dawson 3.834
Bjellqvist 3.986
Wikipedia 3.795
Rodwell 3.63
Grimsley 3.478
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.253
Thurlkill 3.63
EMBOSS 3.795
Sillero 3.948
Patrickios 1.38
IPC_peptide 3.821
IPC2_peptide 3.923
IPC2.peptide.svr19 3.835
Protein with the highest isoelectric point:
>tr|A0A1T2L4W3|A0A1T2L4W3_9GAMM Phosphoribosylformylglycinamidine synthase OS=Solemya pervernicosa gill symbiont OX=642797 GN=purL PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 9.97 RR12 pKa = 11.84 KK13 pKa = 8.91 RR14 pKa = 11.84 VHH16 pKa = 6.24 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTKK25 pKa = 10.06 NGRR28 pKa = 11.84 KK29 pKa = 9.38 VISARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.3 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LCAA44 pKa = 3.92
Molecular weight: 5.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.403
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3236
0
3236
1026818
30
4114
317.3
35.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.193 ± 0.048
0.958 ± 0.022
5.86 ± 0.039
7.076 ± 0.046
3.581 ± 0.026
7.594 ± 0.088
2.382 ± 0.025
6.021 ± 0.034
4.155 ± 0.048
10.506 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.61 ± 0.026
3.567 ± 0.032
4.114 ± 0.033
3.863 ± 0.027
5.998 ± 0.049
6.538 ± 0.053
5.119 ± 0.039
6.932 ± 0.04
1.175 ± 0.018
2.76 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here