Jannaschia pohangensis
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3732 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3I2J2|A0A1I3I2J2_9RHOB Iron-sulfur cluster assembly protein OS=Jannaschia pohangensis OX=390807 GN=SAMN04488095_0792 PE=3 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.36 RR3 pKa = 11.84 HH4 pKa = 5.65 LTLMILPLLATPAFAGNIAPPPAPLEE30 pKa = 4.1 PAPVFVDD37 pKa = 4.29 PAPVGYY43 pKa = 9.58 DD44 pKa = 2.85 WSGFSVGAQIGYY56 pKa = 10.76 ADD58 pKa = 3.95 ANATGGLEE66 pKa = 4.12 GEE68 pKa = 4.6 GAIGGLRR75 pKa = 11.84 ANYY78 pKa = 10.38 DD79 pKa = 3.51 LDD81 pKa = 4.06 LGNWVVGAGIDD92 pKa = 3.93 YY93 pKa = 7.49 DD94 pKa = 4.01 TTNIDD99 pKa = 3.22 LSGAATVDD107 pKa = 2.89 NVLRR111 pKa = 11.84 VKK113 pKa = 10.63 GRR115 pKa = 11.84 AGIDD119 pKa = 3.18 GGRR122 pKa = 11.84 NLYY125 pKa = 10.1 YY126 pKa = 10.16 ATAGYY131 pKa = 9.56 AQAEE135 pKa = 4.25 TDD137 pKa = 5.02 LIGDD141 pKa = 3.56 GDD143 pKa = 4.56 GYY145 pKa = 10.98 FAGLGYY151 pKa = 10.61 EE152 pKa = 4.29 VFVTDD157 pKa = 3.6 QLTVGTEE164 pKa = 3.95 VLYY167 pKa = 11.11 HH168 pKa = 6.48 EE169 pKa = 5.66 FDD171 pKa = 4.7 DD172 pKa = 5.07 FDD174 pKa = 3.99 GAPGVEE180 pKa = 4.16 ADD182 pKa = 3.45 ATTAQVSLNWRR193 pKa = 11.84 FF194 pKa = 3.03
Molecular weight: 20.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.948
Patrickios 1.036
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.825
Protein with the highest isoelectric point:
>tr|A0A1I3LNB6|A0A1I3LNB6_9RHOB NAD(P) transhydrogenase subunit alpha OS=Jannaschia pohangensis OX=390807 GN=SAMN04488095_1580 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.08 AGRR28 pKa = 11.84 RR29 pKa = 11.84 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.97 VLSAA44 pKa = 4.11
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3732
0
3732
1134252
39
3123
303.9
32.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.903 ± 0.059
0.876 ± 0.012
6.524 ± 0.044
5.368 ± 0.036
3.634 ± 0.026
9.01 ± 0.047
1.982 ± 0.023
4.93 ± 0.03
2.53 ± 0.035
10.178 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.728 ± 0.021
2.332 ± 0.022
5.351 ± 0.034
2.932 ± 0.024
7.125 ± 0.044
4.857 ± 0.027
5.841 ± 0.036
7.461 ± 0.029
1.478 ± 0.017
1.962 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here