Amino acid dipepetide frequency for Pseudoalteromonas phage BS5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.164AlaAla: 6.164 ± 1.134
0.73AlaCys: 0.73 ± 0.228
4.785AlaAsp: 4.785 ± 0.597
5.272AlaGlu: 5.272 ± 1.063
3.163AlaPhe: 3.163 ± 0.547
6.732AlaGly: 6.732 ± 0.972
0.811AlaHis: 0.811 ± 0.311
5.434AlaIle: 5.434 ± 0.81
4.542AlaLys: 4.542 ± 0.685
7.461AlaLeu: 7.461 ± 1.194
2.676AlaMet: 2.676 ± 0.448
4.704AlaAsn: 4.704 ± 0.536
2.109AlaPro: 2.109 ± 0.482
3.569AlaGln: 3.569 ± 0.782
3.082AlaArg: 3.082 ± 0.577
5.758AlaSer: 5.758 ± 0.835
4.542AlaThr: 4.542 ± 0.959
4.704AlaVal: 4.704 ± 0.518
0.973AlaTrp: 0.973 ± 0.254
2.758AlaTyr: 2.758 ± 0.584
0.0AlaXaa: 0.0 ± 0.0
Cys
0.649CysAla: 0.649 ± 0.217
0.243CysCys: 0.243 ± 0.114
0.973CysAsp: 0.973 ± 0.306
0.973CysGlu: 0.973 ± 0.324
0.162CysPhe: 0.162 ± 0.111
0.649CysGly: 0.649 ± 0.233
0.324CysHis: 0.324 ± 0.149
0.973CysIle: 0.973 ± 0.293
1.703CysLys: 1.703 ± 0.383
0.973CysLeu: 0.973 ± 0.285
0.487CysMet: 0.487 ± 0.182
0.568CysAsn: 0.568 ± 0.231
0.162CysPro: 0.162 ± 0.103
0.243CysGln: 0.243 ± 0.156
0.892CysArg: 0.892 ± 0.297
1.054CysSer: 1.054 ± 0.291
0.487CysThr: 0.487 ± 0.227
0.811CysVal: 0.811 ± 0.248
0.162CysTrp: 0.162 ± 0.096
0.568CysTyr: 0.568 ± 0.243
0.0CysXaa: 0.0 ± 0.0
Asp
5.109AspAla: 5.109 ± 0.495
0.973AspCys: 0.973 ± 0.284
4.704AspAsp: 4.704 ± 0.648
4.866AspGlu: 4.866 ± 0.621
2.839AspPhe: 2.839 ± 0.381
6.083AspGly: 6.083 ± 0.79
0.649AspHis: 0.649 ± 0.187
5.677AspIle: 5.677 ± 0.547
4.136AspLys: 4.136 ± 0.587
5.596AspLeu: 5.596 ± 0.68
1.703AspMet: 1.703 ± 0.321
3.244AspAsn: 3.244 ± 0.54
1.541AspPro: 1.541 ± 0.329
1.379AspGln: 1.379 ± 0.32
1.703AspArg: 1.703 ± 0.314
4.542AspSer: 4.542 ± 0.768
4.055AspThr: 4.055 ± 0.575
2.352AspVal: 2.352 ± 0.62
1.054AspTrp: 1.054 ± 0.268
3.406AspTyr: 3.406 ± 0.447
0.0AspXaa: 0.0 ± 0.0
Glu
4.947GluAla: 4.947 ± 0.655
0.324GluCys: 0.324 ± 0.154
3.325GluAsp: 3.325 ± 0.458
3.487GluGlu: 3.487 ± 0.548
3.163GluPhe: 3.163 ± 0.443
3.325GluGly: 3.325 ± 0.457
1.217GluHis: 1.217 ± 0.301
3.731GluIle: 3.731 ± 0.53
3.974GluLys: 3.974 ± 0.837
7.543GluLeu: 7.543 ± 0.9
2.433GluMet: 2.433 ± 0.514
4.055GluAsn: 4.055 ± 0.56
1.622GluPro: 1.622 ± 0.38
3.487GluGln: 3.487 ± 0.734
3.082GluArg: 3.082 ± 0.686
4.136GluSer: 4.136 ± 0.589
3.244GluThr: 3.244 ± 0.47
3.082GluVal: 3.082 ± 0.433
1.217GluTrp: 1.217 ± 0.311
1.946GluTyr: 1.946 ± 0.406
0.0GluXaa: 0.0 ± 0.0
Phe
3.406PheAla: 3.406 ± 0.518
0.811PheCys: 0.811 ± 0.275
3.082PheAsp: 3.082 ± 0.509
2.595PheGlu: 2.595 ± 0.366
1.135PhePhe: 1.135 ± 0.349
3.163PheGly: 3.163 ± 0.436
0.324PheHis: 0.324 ± 0.17
2.352PheIle: 2.352 ± 0.301
3.001PheLys: 3.001 ± 0.462
1.784PheLeu: 1.784 ± 0.376
1.379PheMet: 1.379 ± 0.32
3.65PheAsn: 3.65 ± 0.636
0.811PhePro: 0.811 ± 0.21
0.73PheGln: 0.73 ± 0.213
1.379PheArg: 1.379 ± 0.426
2.352PheSer: 2.352 ± 0.396
2.595PheThr: 2.595 ± 0.502
2.028PheVal: 2.028 ± 0.393
0.243PheTrp: 0.243 ± 0.168
1.946PheTyr: 1.946 ± 0.379
0.0PheXaa: 0.0 ± 0.0
Gly
5.758GlyAla: 5.758 ± 0.768
0.973GlyCys: 0.973 ± 0.328
4.623GlyAsp: 4.623 ± 0.517
4.38GlyGlu: 4.38 ± 0.602
2.92GlyPhe: 2.92 ± 0.424
6.326GlyGly: 6.326 ± 1.212
0.73GlyHis: 0.73 ± 0.24
3.406GlyIle: 3.406 ± 0.363
3.974GlyLys: 3.974 ± 0.578
6.732GlyLeu: 6.732 ± 0.724
1.541GlyMet: 1.541 ± 0.342
4.055GlyAsn: 4.055 ± 0.49
0.568GlyPro: 0.568 ± 0.191
2.758GlyGln: 2.758 ± 0.524
2.352GlyArg: 2.352 ± 0.465
5.758GlySer: 5.758 ± 0.804
4.38GlyThr: 4.38 ± 0.678
5.596GlyVal: 5.596 ± 0.717
0.649GlyTrp: 0.649 ± 0.207
3.65GlyTyr: 3.65 ± 0.679
0.0GlyXaa: 0.0 ± 0.0
His
0.811HisAla: 0.811 ± 0.27
0.243HisCys: 0.243 ± 0.122
1.217HisAsp: 1.217 ± 0.35
1.217HisGlu: 1.217 ± 0.339
0.973HisPhe: 0.973 ± 0.306
0.973HisGly: 0.973 ± 0.221
0.081HisHis: 0.081 ± 0.089
0.487HisIle: 0.487 ± 0.188
1.054HisLys: 1.054 ± 0.263
0.811HisLeu: 0.811 ± 0.321
0.162HisMet: 0.162 ± 0.109
0.568HisAsn: 0.568 ± 0.197
0.568HisPro: 0.568 ± 0.187
0.406HisGln: 0.406 ± 0.227
0.243HisArg: 0.243 ± 0.133
0.811HisSer: 0.811 ± 0.3
0.892HisThr: 0.892 ± 0.303
0.487HisVal: 0.487 ± 0.173
0.162HisTrp: 0.162 ± 0.112
0.811HisTyr: 0.811 ± 0.271
0.0HisXaa: 0.0 ± 0.0
Ile
4.785IleAla: 4.785 ± 0.685
0.649IleCys: 0.649 ± 0.269
5.921IleAsp: 5.921 ± 0.813
6.164IleGlu: 6.164 ± 0.598
1.541IlePhe: 1.541 ± 0.332
3.487IleGly: 3.487 ± 0.455
0.811IleHis: 0.811 ± 0.242
4.055IleIle: 4.055 ± 0.842
5.596IleLys: 5.596 ± 0.675
3.893IleLeu: 3.893 ± 0.587
1.054IleMet: 1.054 ± 0.324
4.542IleAsn: 4.542 ± 0.538
2.109IlePro: 2.109 ± 0.462
1.946IleGln: 1.946 ± 0.279
2.109IleArg: 2.109 ± 0.392
5.191IleSer: 5.191 ± 0.606
4.866IleThr: 4.866 ± 0.594
3.569IleVal: 3.569 ± 0.486
0.811IleTrp: 0.811 ± 0.287
1.865IleTyr: 1.865 ± 0.377
0.0IleXaa: 0.0 ± 0.0
Lys
6.002LysAla: 6.002 ± 0.653
0.892LysCys: 0.892 ± 0.253
3.731LysAsp: 3.731 ± 0.577
4.542LysGlu: 4.542 ± 0.696
2.839LysPhe: 2.839 ± 0.448
3.974LysGly: 3.974 ± 0.492
0.811LysHis: 0.811 ± 0.301
3.163LysIle: 3.163 ± 0.533
5.109LysLys: 5.109 ± 0.731
5.515LysLeu: 5.515 ± 0.799
1.784LysMet: 1.784 ± 0.388
2.839LysAsn: 2.839 ± 0.491
2.676LysPro: 2.676 ± 0.475
5.272LysGln: 5.272 ± 0.969
4.136LysArg: 4.136 ± 0.723
3.487LysSer: 3.487 ± 0.574
4.136LysThr: 4.136 ± 0.623
5.109LysVal: 5.109 ± 0.691
1.379LysTrp: 1.379 ± 0.338
2.758LysTyr: 2.758 ± 0.619
0.0LysXaa: 0.0 ± 0.0
Leu
6.488LeuAla: 6.488 ± 1.195
0.892LeuCys: 0.892 ± 0.323
5.109LeuAsp: 5.109 ± 0.527
4.461LeuGlu: 4.461 ± 0.607
3.812LeuPhe: 3.812 ± 0.558
5.515LeuGly: 5.515 ± 0.718
1.298LeuHis: 1.298 ± 0.299
5.596LeuIle: 5.596 ± 0.682
6.732LeuLys: 6.732 ± 0.756
5.272LeuLeu: 5.272 ± 0.763
2.514LeuMet: 2.514 ± 0.5
5.272LeuAsn: 5.272 ± 0.533
2.676LeuPro: 2.676 ± 0.453
3.082LeuGln: 3.082 ± 0.444
2.758LeuArg: 2.758 ± 0.4
7.705LeuSer: 7.705 ± 0.744
5.596LeuThr: 5.596 ± 0.649
3.487LeuVal: 3.487 ± 0.569
0.568LeuTrp: 0.568 ± 0.214
1.703LeuTyr: 1.703 ± 0.299
0.0LeuXaa: 0.0 ± 0.0
Met
2.676MetAla: 2.676 ± 0.508
0.243MetCys: 0.243 ± 0.191
1.135MetAsp: 1.135 ± 0.335
1.054MetGlu: 1.054 ± 0.373
1.054MetPhe: 1.054 ± 0.312
1.135MetGly: 1.135 ± 0.278
0.487MetHis: 0.487 ± 0.149
2.028MetIle: 2.028 ± 0.428
1.784MetLys: 1.784 ± 0.468
1.703MetLeu: 1.703 ± 0.311
0.649MetMet: 0.649 ± 0.285
1.46MetAsn: 1.46 ± 0.336
0.811MetPro: 0.811 ± 0.256
1.541MetGln: 1.541 ± 0.398
1.298MetArg: 1.298 ± 0.404
2.109MetSer: 2.109 ± 0.477
1.784MetThr: 1.784 ± 0.352
1.054MetVal: 1.054 ± 0.312
0.243MetTrp: 0.243 ± 0.129
0.892MetTyr: 0.892 ± 0.276
0.0MetXaa: 0.0 ± 0.0
Asn
4.461AsnAla: 4.461 ± 0.602
0.811AsnCys: 0.811 ± 0.288
4.217AsnAsp: 4.217 ± 0.502
3.487AsnGlu: 3.487 ± 0.486
2.19AsnPhe: 2.19 ± 0.405
4.136AsnGly: 4.136 ± 0.725
0.892AsnHis: 0.892 ± 0.26
3.893AsnIle: 3.893 ± 0.602
3.487AsnLys: 3.487 ± 0.607
5.191AsnLeu: 5.191 ± 0.673
1.217AsnMet: 1.217 ± 0.272
4.217AsnAsn: 4.217 ± 0.556
2.271AsnPro: 2.271 ± 0.428
2.514AsnGln: 2.514 ± 0.529
2.433AsnArg: 2.433 ± 0.384
4.866AsnSer: 4.866 ± 0.506
2.92AsnThr: 2.92 ± 0.39
3.163AsnVal: 3.163 ± 0.423
1.135AsnTrp: 1.135 ± 0.32
2.19AsnTyr: 2.19 ± 0.386
0.0AsnXaa: 0.0 ± 0.0
Pro
1.784ProAla: 1.784 ± 0.292
0.568ProCys: 0.568 ± 0.212
2.19ProAsp: 2.19 ± 0.448
1.946ProGlu: 1.946 ± 0.334
1.135ProPhe: 1.135 ± 0.265
0.973ProGly: 0.973 ± 0.37
0.243ProHis: 0.243 ± 0.128
1.541ProIle: 1.541 ± 0.333
2.514ProLys: 2.514 ± 0.501
1.784ProLeu: 1.784 ± 0.375
0.487ProMet: 0.487 ± 0.215
1.946ProAsn: 1.946 ± 0.324
0.649ProPro: 0.649 ± 0.248
0.973ProGln: 0.973 ± 0.48
0.892ProArg: 0.892 ± 0.319
1.946ProSer: 1.946 ± 0.32
1.946ProThr: 1.946 ± 0.396
2.19ProVal: 2.19 ± 0.405
0.324ProTrp: 0.324 ± 0.149
1.784ProTyr: 1.784 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
3.65GlnAla: 3.65 ± 0.937
0.406GlnCys: 0.406 ± 0.23
2.352GlnAsp: 2.352 ± 0.375
1.784GlnGlu: 1.784 ± 0.449
1.135GlnPhe: 1.135 ± 0.245
2.514GlnGly: 2.514 ± 0.531
0.649GlnHis: 0.649 ± 0.286
3.244GlnIle: 3.244 ± 0.474
2.676GlnLys: 2.676 ± 0.583
4.785GlnLeu: 4.785 ± 0.838
0.811GlnMet: 0.811 ± 0.287
2.19GlnAsn: 2.19 ± 0.362
1.217GlnPro: 1.217 ± 0.249
2.352GlnGln: 2.352 ± 0.567
2.271GlnArg: 2.271 ± 0.488
3.325GlnSer: 3.325 ± 0.539
1.784GlnThr: 1.784 ± 0.35
2.514GlnVal: 2.514 ± 0.351
0.162GlnTrp: 0.162 ± 0.116
1.298GlnTyr: 1.298 ± 0.326
0.0GlnXaa: 0.0 ± 0.0
Arg
3.001ArgAla: 3.001 ± 0.426
0.406ArgCys: 0.406 ± 0.199
3.001ArgAsp: 3.001 ± 0.554
2.839ArgGlu: 2.839 ± 0.6
1.217ArgPhe: 1.217 ± 0.313
2.514ArgGly: 2.514 ± 0.459
0.324ArgHis: 0.324 ± 0.148
3.487ArgIle: 3.487 ± 0.481
3.082ArgLys: 3.082 ± 0.497
3.974ArgLeu: 3.974 ± 0.55
0.811ArgMet: 0.811 ± 0.259
2.271ArgAsn: 2.271 ± 0.399
1.135ArgPro: 1.135 ± 0.234
2.028ArgGln: 2.028 ± 0.448
1.379ArgArg: 1.379 ± 0.31
2.352ArgSer: 2.352 ± 0.351
1.784ArgThr: 1.784 ± 0.459
2.595ArgVal: 2.595 ± 0.464
0.487ArgTrp: 0.487 ± 0.218
0.973ArgTyr: 0.973 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
6.083SerAla: 6.083 ± 0.782
1.054SerCys: 1.054 ± 0.306
4.704SerAsp: 4.704 ± 0.559
4.785SerGlu: 4.785 ± 0.61
2.92SerPhe: 2.92 ± 0.403
6.245SerGly: 6.245 ± 0.874
0.973SerHis: 0.973 ± 0.253
5.353SerIle: 5.353 ± 0.583
4.623SerLys: 4.623 ± 0.619
5.677SerLeu: 5.677 ± 0.823
2.028SerMet: 2.028 ± 0.418
4.623SerAsn: 4.623 ± 0.619
1.784SerPro: 1.784 ± 0.259
3.325SerGln: 3.325 ± 0.532
2.595SerArg: 2.595 ± 0.425
5.191SerSer: 5.191 ± 1.207
5.272SerThr: 5.272 ± 0.696
4.38SerVal: 4.38 ± 0.508
0.568SerTrp: 0.568 ± 0.228
1.865SerTyr: 1.865 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
5.434ThrAla: 5.434 ± 0.882
0.811ThrCys: 0.811 ± 0.265
3.731ThrAsp: 3.731 ± 0.467
2.92ThrGlu: 2.92 ± 0.431
2.352ThrPhe: 2.352 ± 0.395
5.272ThrGly: 5.272 ± 0.756
0.892ThrHis: 0.892 ± 0.219
3.731ThrIle: 3.731 ± 0.489
3.974ThrLys: 3.974 ± 0.601
4.704ThrLeu: 4.704 ± 0.599
0.892ThrMet: 0.892 ± 0.256
3.65ThrAsn: 3.65 ± 0.608
2.433ThrPro: 2.433 ± 0.408
2.676ThrGln: 2.676 ± 0.511
2.19ThrArg: 2.19 ± 0.381
4.623ThrSer: 4.623 ± 0.887
4.217ThrThr: 4.217 ± 0.676
5.272ThrVal: 5.272 ± 0.845
0.487ThrTrp: 0.487 ± 0.211
2.758ThrTyr: 2.758 ± 0.384
0.0ThrXaa: 0.0 ± 0.0
Val
5.434ValAla: 5.434 ± 0.739
1.298ValCys: 1.298 ± 0.41
3.974ValAsp: 3.974 ± 0.545
3.974ValGlu: 3.974 ± 0.532
2.433ValPhe: 2.433 ± 0.372
4.461ValGly: 4.461 ± 0.546
0.568ValHis: 0.568 ± 0.218
3.65ValIle: 3.65 ± 0.484
4.136ValLys: 4.136 ± 0.54
3.487ValLeu: 3.487 ± 0.504
0.892ValMet: 0.892 ± 0.289
3.569ValAsn: 3.569 ± 0.505
1.054ValPro: 1.054 ± 0.334
1.217ValGln: 1.217 ± 0.252
2.19ValArg: 2.19 ± 0.365
4.461ValSer: 4.461 ± 0.588
5.272ValThr: 5.272 ± 0.93
4.217ValVal: 4.217 ± 0.577
0.487ValTrp: 0.487 ± 0.154
2.514ValTyr: 2.514 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
0.568TrpAla: 0.568 ± 0.215
0.243TrpCys: 0.243 ± 0.12
0.487TrpAsp: 0.487 ± 0.202
0.892TrpGlu: 0.892 ± 0.243
0.324TrpPhe: 0.324 ± 0.186
0.649TrpGly: 0.649 ± 0.204
0.081TrpHis: 0.081 ± 0.077
0.487TrpIle: 0.487 ± 0.195
0.406TrpLys: 0.406 ± 0.18
1.379TrpLeu: 1.379 ± 0.326
0.568TrpMet: 0.568 ± 0.225
0.73TrpAsn: 0.73 ± 0.282
0.243TrpPro: 0.243 ± 0.162
0.487TrpGln: 0.487 ± 0.213
0.649TrpArg: 0.649 ± 0.204
1.298TrpSer: 1.298 ± 0.34
0.487TrpThr: 0.487 ± 0.195
0.649TrpVal: 0.649 ± 0.218
0.406TrpTrp: 0.406 ± 0.161
0.811TrpTyr: 0.811 ± 0.249
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.758TyrAla: 2.758 ± 0.48
0.487TyrCys: 0.487 ± 0.217
2.433TyrAsp: 2.433 ± 0.495
1.703TyrGlu: 1.703 ± 0.355
1.46TyrPhe: 1.46 ± 0.304
3.001TyrGly: 3.001 ± 0.471
0.811TyrHis: 0.811 ± 0.254
2.676TyrIle: 2.676 ± 0.54
3.65TyrLys: 3.65 ± 0.656
2.271TyrLeu: 2.271 ± 0.423
0.973TyrMet: 0.973 ± 0.263
1.541TyrAsn: 1.541 ± 0.282
1.379TyrPro: 1.379 ± 0.315
1.135TyrGln: 1.135 ± 0.271
2.028TyrArg: 2.028 ± 0.465
3.082TyrSer: 3.082 ± 0.443
2.758TyrThr: 2.758 ± 0.587
2.028TyrVal: 2.028 ± 0.374
0.324TyrTrp: 0.324 ± 0.205
1.379TyrTyr: 1.379 ± 0.369
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 65 proteins (12331 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski