Drosophila melanogaster totivirus SW-2009a
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D0U493|D0U493_9VIRU RNA-directed RNA polymerase OS=Drosophila melanogaster totivirus SW-2009a OX=663282 PE=3 SV=1
MM1 pKa = 6.89 KK2 pKa = 10.44 QEE4 pKa = 3.86 TRR6 pKa = 11.84 VPQSHH11 pKa = 6.64 IEE13 pKa = 3.98 YY14 pKa = 9.74 PDD16 pKa = 3.35 IEE18 pKa = 4.11 MHH20 pKa = 7.19 LISEE24 pKa = 4.75 NKK26 pKa = 8.19 QQQPSAPMPHH36 pKa = 6.93 EE37 pKa = 4.2 PRR39 pKa = 11.84 AAGQALKK46 pKa = 10.6 DD47 pKa = 3.8 LNLAYY52 pKa = 10.77 LNNDD56 pKa = 2.78 QKK58 pKa = 8.72 TTLSAWVRR66 pKa = 11.84 GLTVEE71 pKa = 4.44 GVKK74 pKa = 10.39 PNPGPSIIEE83 pKa = 4.22 PNHH86 pKa = 6.0 GPSDD90 pKa = 3.91 EE91 pKa = 4.14 LTQAEE96 pKa = 4.29 IQILQRR102 pKa = 11.84 QLAKK106 pKa = 10.08 QDD108 pKa = 3.59 QKK110 pKa = 11.1 PALIMKK116 pKa = 9.65 DD117 pKa = 3.56 QEE119 pKa = 4.24 NLLDD123 pKa = 5.66 DD124 pKa = 3.98 LTEE127 pKa = 4.49 LKK129 pKa = 8.3 TTVQPQIDD137 pKa = 3.62 NSGFLAHH144 pKa = 6.66 VDD146 pKa = 3.43 HH147 pKa = 6.93 AAEE150 pKa = 4.03 FSGTKK155 pKa = 10.18 GEE157 pKa = 4.34 YY158 pKa = 8.96 FAVMKK163 pKa = 10.61 YY164 pKa = 10.35 ILTNSHH170 pKa = 6.07 SNRR173 pKa = 11.84 FACLANLTDD182 pKa = 4.69 IPDD185 pKa = 3.8 EE186 pKa = 4.1 PVEE189 pKa = 4.21 AFVPMSFSRR198 pKa = 11.84 PKK200 pKa = 10.31 KK201 pKa = 9.32 EE202 pKa = 4.2 KK203 pKa = 10.25 KK204 pKa = 10.19 EE205 pKa = 4.02 KK206 pKa = 10.03 DD207 pKa = 2.95 NHH209 pKa = 5.28 MEE211 pKa = 4.04 EE212 pKa = 4.36 KK213 pKa = 10.21 KK214 pKa = 10.66 KK215 pKa = 10.49 KK216 pKa = 9.08 EE217 pKa = 3.95 KK218 pKa = 10.1 RR219 pKa = 11.84 EE220 pKa = 4.03 SMAMPASQKK229 pKa = 10.47 PKK231 pKa = 10.64 LKK233 pKa = 9.69 TDD235 pKa = 3.39 EE236 pKa = 4.19 EE237 pKa = 4.76 KK238 pKa = 10.8 EE239 pKa = 3.96 IEE241 pKa = 4.33 RR242 pKa = 11.84 KK243 pKa = 9.64 NVIRR247 pKa = 11.84 RR248 pKa = 11.84 IVEE251 pKa = 3.57 QFRR254 pKa = 11.84 DD255 pKa = 3.15 KK256 pKa = 11.37 KK257 pKa = 10.23 MYY259 pKa = 10.8 KK260 pKa = 10.06 LVLWLDD266 pKa = 3.47 KK267 pKa = 11.06 PMSRR271 pKa = 11.84 KK272 pKa = 9.45 FKK274 pKa = 10.83 TEE276 pKa = 3.62 VLDD279 pKa = 4.09 EE280 pKa = 4.27 LWGSGWQEE288 pKa = 3.6 RR289 pKa = 11.84 NKK291 pKa = 9.66 MNQYY295 pKa = 9.59 EE296 pKa = 4.15 WLSYY300 pKa = 10.35 CWANNVTKK308 pKa = 10.58 VDD310 pKa = 3.71 TQTVLLSYY318 pKa = 10.07 DD319 pKa = 3.91 IKK321 pKa = 10.22 WQQLPADD328 pKa = 4.04 MKK330 pKa = 10.38 MAILGDD336 pKa = 3.66 SRR338 pKa = 11.84 ADD340 pKa = 3.4 LEE342 pKa = 4.55 AQKK345 pKa = 8.64 THH347 pKa = 5.89 NKK349 pKa = 8.81 VMHH352 pKa = 7.21 AYY354 pKa = 9.9 NGNPLCQGFQEE365 pKa = 4.51 VEE367 pKa = 3.65 ASKK370 pKa = 9.79 TFLNIAEE377 pKa = 4.5 EE378 pKa = 4.56 SNSVLKK384 pKa = 10.52 PYY386 pKa = 10.08 TGLEE390 pKa = 3.77 AEE392 pKa = 4.69 KK393 pKa = 10.73 YY394 pKa = 7.14 ITNIVGDD401 pKa = 4.2 ANPNQSRR408 pKa = 11.84 IFDD411 pKa = 3.61 QDD413 pKa = 3.1 RR414 pKa = 11.84 LRR416 pKa = 11.84 GNQYY420 pKa = 9.2 NANGAVVHH428 pKa = 6.09 NAVSTIPFTNLIPRR442 pKa = 11.84 TIRR445 pKa = 11.84 SDD447 pKa = 3.8 DD448 pKa = 3.69 DD449 pKa = 3.78 VLEE452 pKa = 4.42 KK453 pKa = 10.62 SANRR457 pKa = 11.84 LQVTEE462 pKa = 4.32 TNVTDD467 pKa = 4.14 YY468 pKa = 10.58 YY469 pKa = 11.3 VNPIEE474 pKa = 4.16 PTEE477 pKa = 3.93 LSKK480 pKa = 10.04 TISDD484 pKa = 3.69 QIKK487 pKa = 10.47 NNQSSNWRR495 pKa = 11.84 RR496 pKa = 11.84 DD497 pKa = 3.54 NTSLAGFNSFDD508 pKa = 3.16 IATVNTALIARR519 pKa = 11.84 GLSTEE524 pKa = 4.11 SMTLKK529 pKa = 10.92 LEE531 pKa = 4.26 LLHH534 pKa = 7.74 GIMAMQVEE542 pKa = 4.7 APMINSSTYY551 pKa = 10.67 SIVDD555 pKa = 3.06 NHH557 pKa = 7.11 TIPTVTDD564 pKa = 3.49 RR565 pKa = 11.84 AVIGINDD572 pKa = 3.75 SPVFGEE578 pKa = 4.57 DD579 pKa = 3.2 CGGDD583 pKa = 3.36 LPEE586 pKa = 4.4 YY587 pKa = 9.92 PFGGGTGTIAFHH599 pKa = 6.3 LTLQTVPEE607 pKa = 4.17 EE608 pKa = 4.3 RR609 pKa = 11.84 RR610 pKa = 11.84 DD611 pKa = 3.66 KK612 pKa = 11.33 AIFCPPGLLQAARR625 pKa = 11.84 DD626 pKa = 3.86 GAEE629 pKa = 3.79 ALALFVLSMSEE640 pKa = 4.06 WPFGIYY646 pKa = 9.27 TVTKK650 pKa = 9.31 RR651 pKa = 11.84 TTDD654 pKa = 3.27 EE655 pKa = 4.49 KK656 pKa = 11.33 GLNPADD662 pKa = 3.56 QVYY665 pKa = 9.54 VPMEE669 pKa = 4.27 TITRR673 pKa = 11.84 VGGDD677 pKa = 2.99 RR678 pKa = 11.84 VLDD681 pKa = 3.54 VVLPRR686 pKa = 11.84 RR687 pKa = 11.84 YY688 pKa = 9.91 AVANPTTQGNANALAVIQPQAGPLANGADD717 pKa = 3.57 GLAAGEE723 pKa = 4.11 LLDD726 pKa = 4.34 VNFIGADD733 pKa = 4.14 GITEE737 pKa = 4.14 YY738 pKa = 10.74 PLTYY742 pKa = 10.73 YY743 pKa = 10.9 LYY745 pKa = 8.59 TWALRR750 pKa = 11.84 FDD752 pKa = 3.36 ITTIRR757 pKa = 11.84 QYY759 pKa = 10.21 IGRR762 pKa = 11.84 MAALIGVKK770 pKa = 9.54 HH771 pKa = 5.6 QLWASHH777 pKa = 6.73 EE778 pKa = 4.06 IRR780 pKa = 11.84 VALCQVAPKK789 pKa = 9.67 MVVGVTGSGDD799 pKa = 3.66 LPRR802 pKa = 11.84 GSAAASEE809 pKa = 4.6 VCYY812 pKa = 10.84 SSLLEE817 pKa = 4.01 VSRR820 pKa = 11.84 SEE822 pKa = 4.27 EE823 pKa = 4.01 DD824 pKa = 3.9 FPLLGQVQADD834 pKa = 3.24 FRR836 pKa = 11.84 VFEE839 pKa = 4.81 TNTSTWNKK847 pKa = 9.39 VVLGLATAPNVTSEE861 pKa = 4.37 QNMHH865 pKa = 5.7 VPFVVGDD872 pKa = 3.78 PRR874 pKa = 11.84 SNAWDD879 pKa = 3.59 RR880 pKa = 11.84 LEE882 pKa = 4.45 AVPIAAAWQMYY893 pKa = 6.68 YY894 pKa = 10.45 HH895 pKa = 6.41 SRR897 pKa = 11.84 GVTTAAWNDD906 pKa = 3.09 AYY908 pKa = 10.27 TNVNNVWLQKK918 pKa = 8.54 MARR921 pKa = 11.84 DD922 pKa = 4.0 SFSTTQSTGTILPARR937 pKa = 11.84 YY938 pKa = 9.39 GKK940 pKa = 9.62 IVKK943 pKa = 10.02 NLMRR947 pKa = 11.84 NMFEE951 pKa = 4.13 RR952 pKa = 11.84 EE953 pKa = 3.63 PAKK956 pKa = 10.7 VVTSVGGDD964 pKa = 3.28 EE965 pKa = 4.35 YY966 pKa = 10.9 EE967 pKa = 3.96 ITHH970 pKa = 6.06 FEE972 pKa = 3.49 RR973 pKa = 11.84 WLPGNRR979 pKa = 11.84 YY980 pKa = 9.98 ASVFEE985 pKa = 3.95 QDD987 pKa = 3.13 EE988 pKa = 4.89 TEE990 pKa = 4.38 VNLFPPTLLPDD1001 pKa = 3.26 IWVQYY1006 pKa = 9.28 PATHH1010 pKa = 5.98 TPIMCASFPPVFGQDD1025 pKa = 3.01 STQGFGKK1032 pKa = 10.14 EE1033 pKa = 3.93 SQLIPFRR1040 pKa = 11.84 NANNNLVAPYY1050 pKa = 10.6 VEE1052 pKa = 4.88 AFVANQAYY1060 pKa = 9.29 FPIGSGPNINDD1071 pKa = 3.06 KK1072 pKa = 10.7 VLWNSRR1078 pKa = 11.84 LWMTSGFVQYY1088 pKa = 11.11 LDD1090 pKa = 3.66 YY1091 pKa = 11.26 AGNAINEE1098 pKa = 4.32 VVPAAGLPLGRR1109 pKa = 11.84 SIPLLPGEE1117 pKa = 4.24 VQPVGNTNMSTSCVPRR1133 pKa = 11.84 YY1134 pKa = 10.08 SVDD1137 pKa = 2.53 GRR1139 pKa = 11.84 RR1140 pKa = 11.84 IFTYY1144 pKa = 10.65 VNTAQSVPLIQACNRR1159 pKa = 11.84 ANRR1162 pKa = 11.84 LARR1165 pKa = 11.84 SAWLLLHH1172 pKa = 6.65 VYY1174 pKa = 9.9 IEE1176 pKa = 4.77 PEE1178 pKa = 3.97 LQLLSDD1184 pKa = 4.04 EE1185 pKa = 4.58 VVDD1188 pKa = 5.88 IFDD1191 pKa = 3.65 QLTSKK1196 pKa = 9.66 TFLDD1200 pKa = 4.15 VAKK1203 pKa = 10.4 SAADD1207 pKa = 3.36 SAEE1210 pKa = 3.88 GNIPATKK1217 pKa = 10.53 VLTDD1221 pKa = 3.86 LQAVDD1226 pKa = 4.1 SATLPSTLDD1235 pKa = 3.39 PSTSMLQPAPLLGEE1249 pKa = 4.05 PTTNN1253 pKa = 3.23
Molecular weight: 139.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.158
IPC2_protein 5.181
IPC_protein 5.156
Toseland 5.156
ProMoST 5.321
Dawson 5.181
Bjellqvist 5.296
Wikipedia 5.054
Rodwell 5.092
Grimsley 5.092
Solomon 5.181
Lehninger 5.143
Nozaki 5.308
DTASelect 5.461
Thurlkill 5.156
EMBOSS 5.118
Sillero 5.385
Patrickios 4.139
IPC_peptide 5.181
IPC2_peptide 5.385
IPC2.peptide.svr19 5.313
Protein with the highest isoelectric point:
>tr|D0U493|D0U493_9VIRU RNA-directed RNA polymerase OS=Drosophila melanogaster totivirus SW-2009a OX=663282 PE=3 SV=1
MM1 pKa = 7.05 THH3 pKa = 6.09 QEE5 pKa = 3.79 HH6 pKa = 6.58 KK7 pKa = 10.66 KK8 pKa = 9.69 YY9 pKa = 10.57 ISFEE13 pKa = 4.23 KK14 pKa = 10.24 YY15 pKa = 9.36 VKK17 pKa = 9.6 EE18 pKa = 4.59 GYY20 pKa = 9.32 WITSGSSSIGKK31 pKa = 8.79 VNWSYY36 pKa = 11.89 DD37 pKa = 3.13 GDD39 pKa = 3.97 LGKK42 pKa = 10.88 FKK44 pKa = 10.86 ARR46 pKa = 11.84 KK47 pKa = 9.51 NMLLDD52 pKa = 5.04 LYY54 pKa = 10.49 TPDD57 pKa = 4.89 EE58 pKa = 4.41 IYY60 pKa = 11.38 KK61 pKa = 9.48 MAVEE65 pKa = 4.03 WDD67 pKa = 3.45 GKK69 pKa = 10.43 LEE71 pKa = 3.81 NRR73 pKa = 11.84 VFIKK77 pKa = 10.83 DD78 pKa = 3.4 EE79 pKa = 3.9 LAKK82 pKa = 10.52 RR83 pKa = 11.84 RR84 pKa = 11.84 LAVASNIEE92 pKa = 3.91 AYY94 pKa = 10.63 LNQGYY99 pKa = 9.69 IFYY102 pKa = 10.82 LFGHH106 pKa = 6.32 GFKK109 pKa = 9.7 NWKK112 pKa = 10.03 YY113 pKa = 8.49 ITLDD117 pKa = 3.32 EE118 pKa = 4.74 TPGEE122 pKa = 4.12 THH124 pKa = 6.82 LRR126 pKa = 11.84 NCEE129 pKa = 3.98 TIKK132 pKa = 10.85 ALKK135 pKa = 9.81 DD136 pKa = 3.13 GCFALPFDD144 pKa = 4.61 FKK146 pKa = 11.44 GFDD149 pKa = 3.41 RR150 pKa = 11.84 QPTIWEE156 pKa = 4.11 VKK158 pKa = 10.0 QITKK162 pKa = 10.46 RR163 pKa = 11.84 VIDD166 pKa = 3.72 QIKK169 pKa = 10.32 YY170 pKa = 9.66 LVPPQDD176 pKa = 2.55 RR177 pKa = 11.84 HH178 pKa = 6.21 LFTKK182 pKa = 10.47 IAAKK186 pKa = 10.47 NISCYY191 pKa = 10.75 DD192 pKa = 3.42 NNYY195 pKa = 10.25 LYY197 pKa = 11.05 SPLTKK202 pKa = 9.32 EE203 pKa = 4.51 TKK205 pKa = 10.03 KK206 pKa = 8.83 QTNGIPSGIRR216 pKa = 11.84 PTSFIGNVWNMITTDD231 pKa = 3.06 IARR234 pKa = 11.84 DD235 pKa = 3.6 QTSLILGKK243 pKa = 10.63 DD244 pKa = 3.86 NIRR247 pKa = 11.84 LIALKK252 pKa = 10.76 GDD254 pKa = 3.57 DD255 pKa = 4.01 TKK257 pKa = 11.22 ILAKK261 pKa = 10.72 DD262 pKa = 3.91 FMTCLVFRR270 pKa = 11.84 YY271 pKa = 9.71 SYY273 pKa = 10.61 QAINAVGEE281 pKa = 4.03 NSKK284 pKa = 10.79 FGIMQNCCEE293 pKa = 4.26 FLRR296 pKa = 11.84 TEE298 pKa = 3.99 ISTQGVRR305 pKa = 11.84 GWTNRR310 pKa = 11.84 AIPSVTQRR318 pKa = 11.84 KK319 pKa = 7.73 PWNPQPWTANQQVEE333 pKa = 4.78 TTANNIYY340 pKa = 10.46 LLEE343 pKa = 4.32 RR344 pKa = 11.84 RR345 pKa = 11.84 SKK347 pKa = 10.72 KK348 pKa = 9.93 QLDD351 pKa = 3.52 WLHH354 pKa = 5.7 QANKK358 pKa = 9.94 IKK360 pKa = 9.52 WSKK363 pKa = 9.32 YY364 pKa = 7.55 TGQSYY369 pKa = 9.63 HH370 pKa = 6.63 WLHH373 pKa = 5.99 LPKK376 pKa = 10.37 RR377 pKa = 11.84 LGGFGIYY384 pKa = 7.59 EE385 pKa = 3.87 WKK387 pKa = 10.06 GWKK390 pKa = 6.68 TTSKK394 pKa = 10.8 LPLADD399 pKa = 3.62 IPTFDD404 pKa = 3.62 VPGLFSEE411 pKa = 5.33 NINLTWKK418 pKa = 10.49 QMTDD422 pKa = 3.38 EE423 pKa = 4.92 EE424 pKa = 4.44 KK425 pKa = 9.78 TAYY428 pKa = 9.37 QQVEE432 pKa = 4.32 FSMKK436 pKa = 9.91 IAADD440 pKa = 4.9 DD441 pKa = 3.47 IPGPQKK447 pKa = 10.62 HH448 pKa = 6.14 ISSNYY453 pKa = 8.47 IKK455 pKa = 10.53 NLRR458 pKa = 11.84 KK459 pKa = 9.79 LKK461 pKa = 9.99 PVWEE465 pKa = 4.3 KK466 pKa = 10.17 TVVPTYY472 pKa = 10.63 RR473 pKa = 11.84 FYY475 pKa = 10.26 KK476 pKa = 10.29 TEE478 pKa = 4.41 GPTCEE483 pKa = 3.86 NNIWPRR489 pKa = 11.84 RR490 pKa = 11.84 MIQSHH495 pKa = 5.02 EE496 pKa = 4.15 AGPNGMPIFSEE507 pKa = 5.65 FIRR510 pKa = 11.84 QHH512 pKa = 5.74 QIAKK516 pKa = 8.86 KK517 pKa = 9.84 AKK519 pKa = 9.78 IEE521 pKa = 3.99 IPSLKK526 pKa = 10.02 EE527 pKa = 4.61 CLAKK531 pKa = 10.05 WYY533 pKa = 9.7 PEE535 pKa = 3.79 AYY537 pKa = 8.49 KK538 pKa = 10.88 TMTEE542 pKa = 4.11 YY543 pKa = 10.52 EE544 pKa = 3.99 RR545 pKa = 11.84 KK546 pKa = 9.1 GWHH549 pKa = 5.57 RR550 pKa = 11.84 TDD552 pKa = 5.09 AINIATGKK560 pKa = 10.21 VPLEE564 pKa = 3.77 PTGILNPILTPWISKK579 pKa = 9.97 IVVKK583 pKa = 10.66 SGFSFWRR590 pKa = 11.84 GRR592 pKa = 11.84 EE593 pKa = 3.95 NIALHH598 pKa = 5.62 LTSVCKK604 pKa = 10.14 QAVQYY609 pKa = 9.81 IQNEE613 pKa = 4.41 GGNYY617 pKa = 8.8 MYY619 pKa = 11.27 AFF621 pKa = 4.66
Molecular weight: 72.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.793
IPC2_protein 8.799
IPC_protein 8.653
Toseland 9.399
ProMoST 9.18
Dawson 9.677
Bjellqvist 9.414
Wikipedia 9.853
Rodwell 9.984
Grimsley 9.765
Solomon 9.706
Lehninger 9.663
Nozaki 9.516
DTASelect 9.37
Thurlkill 9.531
EMBOSS 9.838
Sillero 9.633
Patrickios 4.787
IPC_peptide 9.692
IPC2_peptide 7.98
IPC2.peptide.svr19 7.826
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1874
621
1253
937.0
105.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.417 ± 1.038
1.014 ± 0.215
5.069 ± 0.435
6.137 ± 0.167
3.575 ± 0.302
5.763 ± 0.176
1.814 ± 0.138
6.083 ± 1.13
6.403 ± 1.607
8.218 ± 0.559
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.348 ± 0.205
6.03 ± 0.194
5.87 ± 0.433
4.803 ± 0.145
4.536 ± 0.013
5.763 ± 0.38
7.044 ± 0.021
6.19 ± 1.147
2.241 ± 0.563
3.682 ± 0.567
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here