Diaporthe ambigua RNA virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9Q9R2|Q9Q9R2_9VIRU ORF1 OS=Diaporthe ambigua RNA virus 1 OX=111470 PE=4 SV=1
MM1 pKa = 7.82RR2 pKa = 11.84FFNEE6 pKa = 4.41LMMTTDD12 pKa = 2.54IATYY16 pKa = 10.21YY17 pKa = 10.97SFASWYY23 pKa = 10.36GRR25 pKa = 11.84TFGYY29 pKa = 10.22GVPFWFIWRR38 pKa = 11.84DD39 pKa = 3.27FTLYY43 pKa = 10.8VGFDD47 pKa = 3.46VPEE50 pKa = 4.09EE51 pKa = 3.9LVAYY55 pKa = 9.66ALSLFSIAWLTTLIYY70 pKa = 9.86FHH72 pKa = 6.95LWATAFVAVAWFLGVVMLVRR92 pKa = 11.84AILRR96 pKa = 11.84FVEE99 pKa = 4.36WFTFGLAPFVGGFVSSSTWVVSCLCRR125 pKa = 11.84ALMYY129 pKa = 10.59LFFYY133 pKa = 10.63LPPPLVAGQLLKK145 pKa = 10.31LTLSLLWSLVWEE157 pKa = 4.94SIWLPVDD164 pKa = 3.78LWHH167 pKa = 7.11LGVGSWRR174 pKa = 11.84LGSVGWDD181 pKa = 3.12GSNRR185 pKa = 11.84VVQLGGFCFRR195 pKa = 11.84IRR197 pKa = 11.84FSDD200 pKa = 3.7FVYY203 pKa = 9.8PYY205 pKa = 10.26RR206 pKa = 11.84VLVLPGFVLGVLHH219 pKa = 6.16VLPGGFWLCFPSFKK233 pKa = 10.67FEE235 pKa = 3.94QLGVLGSLFIYY246 pKa = 10.11PLVEE250 pKa = 3.84TKK252 pKa = 10.78LIFVLGWVDD261 pKa = 3.49VAFVLPWLAAVLVGVSFSYY280 pKa = 10.91RR281 pKa = 11.84EE282 pKa = 4.14VPFSRR287 pKa = 11.84ADD289 pKa = 3.41LQGYY293 pKa = 9.51LGLSEE298 pKa = 4.83PSQDD302 pKa = 3.03ASVLSEE308 pKa = 4.57LDD310 pKa = 3.38ALLKK314 pKa = 10.79AKK316 pKa = 10.46SFADD320 pKa = 3.54AEE322 pKa = 4.67GVALVAAVGARR333 pKa = 11.84GGVPRR338 pKa = 11.84LRR340 pKa = 11.84ARR342 pKa = 11.84GKK344 pKa = 9.56LACRR348 pKa = 11.84LQALLGGRR356 pKa = 11.84KK357 pKa = 9.23GCVGAFFSGRR367 pKa = 11.84WVPDD371 pKa = 3.46LPASDD376 pKa = 4.77RR377 pKa = 11.84SPALNAILAVQRR389 pKa = 11.84EE390 pKa = 4.27QVKK393 pKa = 10.3VLGVGTFPTKK403 pKa = 10.56SGVGGDD409 pKa = 3.79YY410 pKa = 11.22VLVEE414 pKa = 4.32SRR416 pKa = 11.84SGDD419 pKa = 3.1RR420 pKa = 11.84RR421 pKa = 11.84VILPSLLSRR430 pKa = 11.84LCLYY434 pKa = 10.77SSLRR438 pKa = 11.84EE439 pKa = 3.84RR440 pKa = 11.84DD441 pKa = 3.29EE442 pKa = 4.1KK443 pKa = 11.45LLVGLRR449 pKa = 11.84SRR451 pKa = 11.84AVEE454 pKa = 3.85WCRR457 pKa = 11.84LEE459 pKa = 4.98QVPDD463 pKa = 3.87WVPLLCLSSSVADD476 pKa = 3.48AAEE479 pKa = 3.87ISGPEE484 pKa = 3.62VEE486 pKa = 4.0ARR488 pKa = 11.84RR489 pKa = 11.84RR490 pKa = 11.84LRR492 pKa = 11.84SSLGIDD498 pKa = 3.27TLASFF503 pKa = 5.05

Molecular weight: 56.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9Q9R2|Q9Q9R2_9VIRU ORF1 OS=Diaporthe ambigua RNA virus 1 OX=111470 PE=4 SV=1
MM1 pKa = 7.35FVPFPLPCEE10 pKa = 4.47GTWLPQVHH18 pKa = 5.87RR19 pKa = 11.84VCPHH23 pKa = 6.02NEE25 pKa = 3.31VGALCRR31 pKa = 11.84RR32 pKa = 11.84VLAPLPAQVQEE43 pKa = 4.24RR44 pKa = 11.84VSPGFSRR51 pKa = 11.84AMGLAVGLARR61 pKa = 11.84FYY63 pKa = 9.24TQPRR67 pKa = 11.84WSLEE71 pKa = 3.97RR72 pKa = 11.84TALSYY77 pKa = 11.27DD78 pKa = 3.1GALRR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84YY85 pKa = 9.66LGAMDD90 pKa = 5.96SLLDD94 pKa = 3.51WPLDD98 pKa = 3.52VGKK101 pKa = 10.17DD102 pKa = 3.4SRR104 pKa = 11.84LDD106 pKa = 3.53CFLKK110 pKa = 10.84AEE112 pKa = 4.52KK113 pKa = 10.76LNTLIKK119 pKa = 10.16RR120 pKa = 11.84PKK122 pKa = 9.0PRR124 pKa = 11.84LIFPRR129 pKa = 11.84SPRR132 pKa = 11.84YY133 pKa = 9.95NLVLASRR140 pKa = 11.84LKK142 pKa = 10.71PFEE145 pKa = 3.91HH146 pKa = 6.89WLWSHH151 pKa = 6.11LTAEE155 pKa = 4.54RR156 pKa = 11.84LGTSGRR162 pKa = 11.84GRR164 pKa = 11.84YY165 pKa = 7.4VAKK168 pKa = 10.39GLNLSQRR175 pKa = 11.84ARR177 pKa = 11.84LIVKK181 pKa = 9.97KK182 pKa = 9.47MAGFSRR188 pKa = 11.84CAVLEE193 pKa = 3.99VDD195 pKa = 4.33GKK197 pKa = 11.02AFEE200 pKa = 4.27AHH202 pKa = 6.07VGPSSIEE209 pKa = 3.81QEE211 pKa = 3.88HH212 pKa = 5.84RR213 pKa = 11.84VYY215 pKa = 10.66KK216 pKa = 10.35AAYY219 pKa = 8.32PGDD222 pKa = 3.96STLSSLLRR230 pKa = 11.84AQRR233 pKa = 11.84VLKK236 pKa = 10.72GRR238 pKa = 11.84LPCGAKK244 pKa = 10.5FSRR247 pKa = 11.84NGGRR251 pKa = 11.84ASGDD255 pKa = 3.44FNTGMGNSLIMFSCVYY271 pKa = 10.72ASLVEE276 pKa = 4.34LGYY279 pKa = 10.81SSWDD283 pKa = 3.6FLCDD287 pKa = 3.36GDD289 pKa = 4.0NALLFVEE296 pKa = 5.01SSDD299 pKa = 3.79VDD301 pKa = 3.9DD302 pKa = 4.63LVEE305 pKa = 4.34RR306 pKa = 11.84LPGVAVGTCGQEE318 pKa = 3.83LSLDD322 pKa = 3.65AVTGSLSGVVFGQSKK337 pKa = 9.95PVFFPSGWRR346 pKa = 11.84LVRR349 pKa = 11.84DD350 pKa = 3.54PFKK353 pKa = 10.89ALSGFGCSHH362 pKa = 6.39AWLRR366 pKa = 11.84EE367 pKa = 3.64PKK369 pKa = 10.31FRR371 pKa = 11.84DD372 pKa = 3.46EE373 pKa = 4.3YY374 pKa = 10.51LVGVARR380 pKa = 11.84CEE382 pKa = 3.84LALSLGQPLLQAFCLRR398 pKa = 11.84VLALFPGVVPRR409 pKa = 11.84DD410 pKa = 3.34HH411 pKa = 6.97VAFRR415 pKa = 11.84DD416 pKa = 3.86YY417 pKa = 11.31EE418 pKa = 4.22FLGVSPRR425 pKa = 11.84VLRR428 pKa = 11.84RR429 pKa = 11.84PEE431 pKa = 3.82PVTWEE436 pKa = 4.19TRR438 pKa = 11.84VSFADD443 pKa = 3.46AFSISPEE450 pKa = 3.82RR451 pKa = 11.84QLEE454 pKa = 4.22IEE456 pKa = 4.24SSFDD460 pKa = 3.38LVRR463 pKa = 11.84PSGFLASDD471 pKa = 3.38AVDD474 pKa = 3.61SFFGADD480 pKa = 2.91PGMAEE485 pKa = 3.68KK486 pKa = 10.21WFEE489 pKa = 3.81RR490 pKa = 11.84WVV492 pKa = 3.31

Molecular weight: 54.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

995

492

503

497.5

55.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.04 ± 0.063

2.111 ± 0.229

4.422 ± 0.461

4.623 ± 0.462

6.935 ± 0.443

8.643 ± 0.216

1.106 ± 0.364

2.01 ± 0.41

2.714 ± 0.518

14.07 ± 1.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.206 ± 0.151

1.106 ± 0.364

5.628 ± 0.612

2.111 ± 0.229

8.04 ± 0.773

8.643 ± 0.068

2.714 ± 0.192

10.05 ± 1.058

3.116 ± 0.615

2.714 ± 0.334
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski