Roseomonas oryzae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4202 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B2TB29|A0A5B2TB29_9PROT Uncharacterized protein OS=Roseomonas oryzae OX=1608942 GN=F0Q34_18735 PE=4 SV=1
MM1 pKa = 6.86LQVYY5 pKa = 7.49GTLRR9 pKa = 11.84LVFGWGGDD17 pKa = 3.67DD18 pKa = 4.17TITAEE23 pKa = 4.51APPGGIWGNVLFGGWGNDD41 pKa = 2.95TLTAIGDD48 pKa = 3.74RR49 pKa = 11.84NLVFGEE55 pKa = 4.71AGDD58 pKa = 3.8DD59 pKa = 3.99TITLRR64 pKa = 11.84GPLRR68 pKa = 11.84PEE70 pKa = 4.0PYY72 pKa = 10.16GGYY75 pKa = 10.03EE76 pKa = 4.32DD77 pKa = 5.35SLLNTAFGGAGNDD90 pKa = 3.46TLTSHH95 pKa = 6.46GFAATLRR102 pKa = 11.84GGAGSDD108 pKa = 3.33TLRR111 pKa = 11.84LDD113 pKa = 4.62GYY115 pKa = 9.2PGPGGYY121 pKa = 10.41AYY123 pKa = 10.36GDD125 pKa = 3.88EE126 pKa = 4.82GDD128 pKa = 5.3DD129 pKa = 4.09DD130 pKa = 5.11LSNVTDD136 pKa = 3.96GGVLDD141 pKa = 5.05GGAGADD147 pKa = 3.05IFYY150 pKa = 10.49GANFAFSPTTGAGTIMTGGSGADD173 pKa = 3.2KK174 pKa = 10.66FSLFGGGSYY183 pKa = 8.46TVEE186 pKa = 5.25DD187 pKa = 4.07YY188 pKa = 11.39SADD191 pKa = 3.54NRR193 pKa = 11.84LSAGDD198 pKa = 3.95TLTGLISVVTDD209 pKa = 3.83LQAEE213 pKa = 4.16DD214 pKa = 3.59SLYY217 pKa = 10.88FGNGLQLSDD226 pKa = 3.55RR227 pKa = 11.84FEE229 pKa = 5.72GEE231 pKa = 3.98VPLEE235 pKa = 4.16LGVPGYY241 pKa = 10.35SAGLQAILPLGGYY254 pKa = 8.78VAVKK258 pKa = 10.61GDD260 pKa = 3.97LFNPGMFDD268 pKa = 3.56VQEE271 pKa = 5.0DD272 pKa = 4.75GPDD275 pKa = 3.43LLLLFVEE282 pKa = 5.51RR283 pKa = 11.84YY284 pKa = 8.17IGRR287 pKa = 11.84PGGEE291 pKa = 4.02GPDD294 pKa = 3.63FQAASGAVALLDD306 pKa = 3.85YY307 pKa = 8.54TASTIPFAEE316 pKa = 4.3PFIVAA321 pKa = 4.56

Molecular weight: 32.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B2TL10|A0A5B2TL10_9PROT NAD(P)/FAD-dependent oxidoreductase OS=Roseomonas oryzae OX=1608942 GN=F0Q34_05625 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.44GFRR19 pKa = 11.84TRR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.14GRR39 pKa = 11.84KK40 pKa = 8.82KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4202

0

4202

1378811

22

2458

328.1

35.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.362 ± 0.065

0.855 ± 0.011

4.99 ± 0.025

5.59 ± 0.034

3.329 ± 0.022

9.368 ± 0.037

2.081 ± 0.016

4.125 ± 0.029

2.065 ± 0.026

11.239 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.018

2.132 ± 0.02

6.23 ± 0.032

3.184 ± 0.021

8.289 ± 0.036

4.713 ± 0.024

4.657 ± 0.027

7.049 ± 0.03

1.483 ± 0.017

1.783 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski