Amino acid dipepetide frequency for Pseudomonas phage phiPsa300

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.319AlaAla: 8.319 ± 0.668
1.248AlaCys: 1.248 ± 0.239
5.234AlaAsp: 5.234 ± 0.431
5.373AlaGlu: 5.373 ± 0.5
2.808AlaPhe: 2.808 ± 0.306
6.031AlaGly: 6.031 ± 0.453
1.49AlaHis: 1.49 ± 0.262
5.442AlaIle: 5.442 ± 0.419
5.997AlaLys: 5.997 ± 0.542
8.076AlaLeu: 8.076 ± 0.597
2.149AlaMet: 2.149 ± 0.275
3.813AlaAsn: 3.813 ± 0.348
2.496AlaPro: 2.496 ± 0.393
4.263AlaGln: 4.263 ± 0.361
4.159AlaArg: 4.159 ± 0.335
4.679AlaSer: 4.679 ± 0.568
5.719AlaThr: 5.719 ± 0.535
6.066AlaVal: 6.066 ± 0.634
1.421AlaTrp: 1.421 ± 0.229
3.293AlaTyr: 3.293 ± 0.345
0.0AlaXaa: 0.0 ± 0.0
Cys
0.901CysAla: 0.901 ± 0.165
0.381CysCys: 0.381 ± 0.117
1.144CysAsp: 1.144 ± 0.191
0.763CysGlu: 0.763 ± 0.15
0.52CysPhe: 0.52 ± 0.137
0.971CysGly: 0.971 ± 0.209
0.485CysHis: 0.485 ± 0.115
0.416CysIle: 0.416 ± 0.116
0.797CysLys: 0.797 ± 0.174
0.832CysLeu: 0.832 ± 0.177
0.52CysMet: 0.52 ± 0.114
0.936CysAsn: 0.936 ± 0.174
0.797CysPro: 0.797 ± 0.193
0.416CysGln: 0.416 ± 0.108
0.693CysArg: 0.693 ± 0.158
0.485CysSer: 0.485 ± 0.113
0.971CysThr: 0.971 ± 0.185
0.693CysVal: 0.693 ± 0.163
0.0CysTrp: 0.0 ± 0.0
0.659CysTyr: 0.659 ± 0.144
0.0CysXaa: 0.0 ± 0.0
Asp
4.853AspAla: 4.853 ± 0.425
1.04AspCys: 1.04 ± 0.188
3.328AspAsp: 3.328 ± 0.329
3.778AspGlu: 3.778 ± 0.417
3.016AspPhe: 3.016 ± 0.303
4.61AspGly: 4.61 ± 0.353
1.179AspHis: 1.179 ± 0.19
3.293AspIle: 3.293 ± 0.283
4.159AspLys: 4.159 ± 0.433
5.026AspLeu: 5.026 ± 0.353
2.045AspMet: 2.045 ± 0.278
3.016AspAsn: 3.016 ± 0.31
2.184AspPro: 2.184 ± 0.277
2.114AspGln: 2.114 ± 0.276
3.05AspArg: 3.05 ± 0.33
3.501AspSer: 3.501 ± 0.392
3.362AspThr: 3.362 ± 0.328
4.541AspVal: 4.541 ± 0.353
1.179AspTrp: 1.179 ± 0.206
2.426AspTyr: 2.426 ± 0.264
0.0AspXaa: 0.0 ± 0.0
Glu
6.17GluAla: 6.17 ± 0.499
0.971GluCys: 0.971 ± 0.195
4.09GluAsp: 4.09 ± 0.377
5.095GluGlu: 5.095 ± 0.52
3.293GluPhe: 3.293 ± 0.319
3.882GluGly: 3.882 ± 0.412
1.525GluHis: 1.525 ± 0.226
4.021GluIle: 4.021 ± 0.406
3.397GluLys: 3.397 ± 0.415
5.511GluLeu: 5.511 ± 0.514
2.496GluMet: 2.496 ± 0.331
2.565GluAsn: 2.565 ± 0.267
1.525GluPro: 1.525 ± 0.261
2.808GluGln: 2.808 ± 0.293
3.397GluArg: 3.397 ± 0.349
3.432GluSer: 3.432 ± 0.398
3.016GluThr: 3.016 ± 0.302
4.714GluVal: 4.714 ± 0.454
0.971GluTrp: 0.971 ± 0.186
2.288GluTyr: 2.288 ± 0.234
0.0GluXaa: 0.0 ± 0.0
Phe
3.05PheAla: 3.05 ± 0.361
0.243PheCys: 0.243 ± 0.082
2.634PheAsp: 2.634 ± 0.254
3.189PheGlu: 3.189 ± 0.271
1.352PhePhe: 1.352 ± 0.236
3.016PheGly: 3.016 ± 0.35
0.728PheHis: 0.728 ± 0.146
1.941PheIle: 1.941 ± 0.269
2.01PheLys: 2.01 ± 0.233
2.808PheLeu: 2.808 ± 0.305
1.109PheMet: 1.109 ± 0.189
2.253PheAsn: 2.253 ± 0.286
1.594PhePro: 1.594 ± 0.221
1.352PheGln: 1.352 ± 0.212
1.594PheArg: 1.594 ± 0.237
2.461PheSer: 2.461 ± 0.27
2.53PheThr: 2.53 ± 0.324
2.6PheVal: 2.6 ± 0.337
0.312PheTrp: 0.312 ± 0.143
1.49PheTyr: 1.49 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
5.546GlyAla: 5.546 ± 0.571
1.248GlyCys: 1.248 ± 0.192
4.125GlyAsp: 4.125 ± 0.434
4.437GlyGlu: 4.437 ± 0.408
3.258GlyPhe: 3.258 ± 0.3
5.997GlyGly: 5.997 ± 0.634
1.179GlyHis: 1.179 ± 0.254
4.333GlyIle: 4.333 ± 0.36
4.367GlyLys: 4.367 ± 0.431
5.754GlyLeu: 5.754 ± 0.436
2.01GlyMet: 2.01 ± 0.265
3.189GlyAsn: 3.189 ± 0.395
1.594GlyPro: 1.594 ± 0.245
2.565GlyGln: 2.565 ± 0.339
3.293GlyArg: 3.293 ± 0.289
4.957GlySer: 4.957 ± 0.447
4.402GlyThr: 4.402 ± 0.495
5.65GlyVal: 5.65 ± 0.433
1.421GlyTrp: 1.421 ± 0.221
3.57GlyTyr: 3.57 ± 0.397
0.0GlyXaa: 0.0 ± 0.0
His
1.56HisAla: 1.56 ± 0.204
0.208HisCys: 0.208 ± 0.076
1.005HisAsp: 1.005 ± 0.19
1.109HisGlu: 1.109 ± 0.219
1.075HisPhe: 1.075 ± 0.234
1.56HisGly: 1.56 ± 0.231
0.485HisHis: 0.485 ± 0.145
1.144HisIle: 1.144 ± 0.191
1.179HisLys: 1.179 ± 0.242
1.213HisLeu: 1.213 ± 0.198
0.555HisMet: 0.555 ± 0.129
0.797HisAsn: 0.797 ± 0.188
0.797HisPro: 0.797 ± 0.17
0.589HisGln: 0.589 ± 0.164
0.693HisArg: 0.693 ± 0.154
0.901HisSer: 0.901 ± 0.161
1.525HisThr: 1.525 ± 0.216
1.49HisVal: 1.49 ± 0.207
0.52HisTrp: 0.52 ± 0.152
1.179HisTyr: 1.179 ± 0.213
0.0HisXaa: 0.0 ± 0.0
Ile
4.506IleAla: 4.506 ± 0.458
0.797IleCys: 0.797 ± 0.149
3.709IleAsp: 3.709 ± 0.381
3.986IleGlu: 3.986 ± 0.436
1.629IlePhe: 1.629 ± 0.232
4.263IleGly: 4.263 ± 0.432
1.144IleHis: 1.144 ± 0.24
2.773IleIle: 2.773 ± 0.309
4.263IleLys: 4.263 ± 0.365
3.917IleLeu: 3.917 ± 0.345
1.664IleMet: 1.664 ± 0.225
3.362IleAsn: 3.362 ± 0.481
2.322IlePro: 2.322 ± 0.236
2.253IleGln: 2.253 ± 0.285
2.842IleArg: 2.842 ± 0.316
3.154IleSer: 3.154 ± 0.321
4.402IleThr: 4.402 ± 0.393
4.055IleVal: 4.055 ± 0.446
0.589IleTrp: 0.589 ± 0.136
1.594IleTyr: 1.594 ± 0.215
0.0IleXaa: 0.0 ± 0.0
Lys
6.62LysAla: 6.62 ± 0.632
0.589LysCys: 0.589 ± 0.129
3.536LysAsp: 3.536 ± 0.351
4.194LysGlu: 4.194 ± 0.467
1.768LysPhe: 1.768 ± 0.243
4.333LysGly: 4.333 ± 0.449
1.144LysHis: 1.144 ± 0.216
3.744LysIle: 3.744 ± 0.344
3.328LysLys: 3.328 ± 0.545
5.026LysLeu: 5.026 ± 0.456
2.704LysMet: 2.704 ± 0.313
2.045LysAsn: 2.045 ± 0.282
2.392LysPro: 2.392 ± 0.325
2.738LysGln: 2.738 ± 0.299
2.842LysArg: 2.842 ± 0.339
3.154LysSer: 3.154 ± 0.351
2.981LysThr: 2.981 ± 0.318
4.437LysVal: 4.437 ± 0.385
0.832LysTrp: 0.832 ± 0.178
1.976LysTyr: 1.976 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
7.799LeuAla: 7.799 ± 0.638
0.797LeuCys: 0.797 ± 0.148
5.823LeuAsp: 5.823 ± 0.457
5.511LeuGlu: 5.511 ± 0.44
2.981LeuPhe: 2.981 ± 0.372
4.749LeuGly: 4.749 ± 0.423
1.802LeuHis: 1.802 ± 0.255
4.506LeuIle: 4.506 ± 0.332
4.957LeuLys: 4.957 ± 0.484
5.373LeuLeu: 5.373 ± 0.442
2.496LeuMet: 2.496 ± 0.281
3.847LeuAsn: 3.847 ± 0.424
3.57LeuPro: 3.57 ± 0.31
3.501LeuGln: 3.501 ± 0.344
3.501LeuArg: 3.501 ± 0.299
4.263LeuSer: 4.263 ± 0.362
6.101LeuThr: 6.101 ± 0.507
4.263LeuVal: 4.263 ± 0.378
1.075LeuTrp: 1.075 ± 0.193
2.288LeuTyr: 2.288 ± 0.242
0.0LeuXaa: 0.0 ± 0.0
Met
3.744MetAla: 3.744 ± 0.342
0.381MetCys: 0.381 ± 0.125
1.144MetAsp: 1.144 ± 0.187
1.906MetGlu: 1.906 ± 0.292
1.179MetPhe: 1.179 ± 0.203
1.56MetGly: 1.56 ± 0.244
0.485MetHis: 0.485 ± 0.152
1.941MetIle: 1.941 ± 0.272
2.461MetLys: 2.461 ± 0.335
2.322MetLeu: 2.322 ± 0.278
1.075MetMet: 1.075 ± 0.237
1.525MetAsn: 1.525 ± 0.232
0.832MetPro: 0.832 ± 0.18
1.109MetGln: 1.109 ± 0.196
1.179MetArg: 1.179 ± 0.179
1.802MetSer: 1.802 ± 0.284
2.669MetThr: 2.669 ± 0.253
1.802MetVal: 1.802 ± 0.257
0.347MetTrp: 0.347 ± 0.098
0.867MetTyr: 0.867 ± 0.169
0.0MetXaa: 0.0 ± 0.0
Asn
3.917AsnAla: 3.917 ± 0.38
0.485AsnCys: 0.485 ± 0.145
2.773AsnAsp: 2.773 ± 0.342
2.218AsnGlu: 2.218 ± 0.283
1.109AsnPhe: 1.109 ± 0.176
4.471AsnGly: 4.471 ± 0.454
1.04AsnHis: 1.04 ± 0.214
3.016AsnIle: 3.016 ± 0.408
2.704AsnLys: 2.704 ± 0.321
4.125AsnLeu: 4.125 ± 0.521
0.936AsnMet: 0.936 ± 0.157
2.218AsnAsn: 2.218 ± 0.31
2.704AsnPro: 2.704 ± 0.343
1.56AsnGln: 1.56 ± 0.229
2.392AsnArg: 2.392 ± 0.247
2.392AsnSer: 2.392 ± 0.337
3.085AsnThr: 3.085 ± 0.385
3.432AsnVal: 3.432 ± 0.32
0.485AsnTrp: 0.485 ± 0.109
1.525AsnTyr: 1.525 ± 0.226
0.0AsnXaa: 0.0 ± 0.0
Pro
2.877ProAla: 2.877 ± 0.328
0.52ProCys: 0.52 ± 0.127
2.392ProAsp: 2.392 ± 0.285
3.016ProGlu: 3.016 ± 0.311
1.179ProPhe: 1.179 ± 0.185
2.253ProGly: 2.253 ± 0.322
0.797ProHis: 0.797 ± 0.152
1.421ProIle: 1.421 ± 0.233
1.906ProLys: 1.906 ± 0.343
2.842ProLeu: 2.842 ± 0.273
1.109ProMet: 1.109 ± 0.196
1.941ProAsn: 1.941 ± 0.235
0.763ProPro: 0.763 ± 0.153
1.56ProGln: 1.56 ± 0.249
1.664ProArg: 1.664 ± 0.256
1.976ProSer: 1.976 ± 0.253
2.738ProThr: 2.738 ± 0.333
3.224ProVal: 3.224 ± 0.367
0.312ProTrp: 0.312 ± 0.117
1.49ProTyr: 1.49 ± 0.219
0.0ProXaa: 0.0 ± 0.0
Gln
4.298GlnAla: 4.298 ± 0.431
0.347GlnCys: 0.347 ± 0.109
2.184GlnAsp: 2.184 ± 0.271
2.461GlnGlu: 2.461 ± 0.306
1.629GlnPhe: 1.629 ± 0.225
2.565GlnGly: 2.565 ± 0.291
0.763GlnHis: 0.763 ± 0.141
2.461GlnIle: 2.461 ± 0.247
2.288GlnLys: 2.288 ± 0.315
3.605GlnLeu: 3.605 ± 0.349
1.56GlnMet: 1.56 ± 0.198
1.56GlnAsn: 1.56 ± 0.235
1.213GlnPro: 1.213 ± 0.163
1.906GlnGln: 1.906 ± 0.289
2.114GlnArg: 2.114 ± 0.268
1.768GlnSer: 1.768 ± 0.274
1.768GlnThr: 1.768 ± 0.258
2.946GlnVal: 2.946 ± 0.305
0.728GlnTrp: 0.728 ± 0.134
1.802GlnTyr: 1.802 ± 0.217
0.0GlnXaa: 0.0 ± 0.0
Arg
3.432ArgAla: 3.432 ± 0.337
0.624ArgCys: 0.624 ± 0.141
2.946ArgAsp: 2.946 ± 0.346
3.258ArgGlu: 3.258 ± 0.346
1.664ArgPhe: 1.664 ± 0.229
3.258ArgGly: 3.258 ± 0.395
0.728ArgHis: 0.728 ± 0.171
2.773ArgIle: 2.773 ± 0.306
2.808ArgLys: 2.808 ± 0.377
4.471ArgLeu: 4.471 ± 0.388
1.421ArgMet: 1.421 ± 0.207
2.6ArgAsn: 2.6 ± 0.322
1.594ArgPro: 1.594 ± 0.218
1.837ArgGln: 1.837 ± 0.225
2.322ArgArg: 2.322 ± 0.339
2.253ArgSer: 2.253 ± 0.265
3.189ArgThr: 3.189 ± 0.255
3.744ArgVal: 3.744 ± 0.381
0.659ArgTrp: 0.659 ± 0.17
1.49ArgTyr: 1.49 ± 0.257
0.0ArgXaa: 0.0 ± 0.0
Ser
4.679SerAla: 4.679 ± 0.494
0.936SerCys: 0.936 ± 0.236
3.847SerAsp: 3.847 ± 0.316
2.322SerGlu: 2.322 ± 0.279
1.906SerPhe: 1.906 ± 0.229
5.234SerGly: 5.234 ± 0.571
1.144SerHis: 1.144 ± 0.211
2.912SerIle: 2.912 ± 0.311
3.154SerLys: 3.154 ± 0.323
4.367SerLeu: 4.367 ± 0.389
1.248SerMet: 1.248 ± 0.179
2.773SerAsn: 2.773 ± 0.363
2.288SerPro: 2.288 ± 0.314
2.149SerGln: 2.149 ± 0.241
2.634SerArg: 2.634 ± 0.292
3.951SerSer: 3.951 ± 0.911
2.808SerThr: 2.808 ± 0.342
4.09SerVal: 4.09 ± 0.429
0.797SerTrp: 0.797 ± 0.164
2.565SerTyr: 2.565 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
5.65ThrAla: 5.65 ± 0.567
0.728ThrCys: 0.728 ± 0.163
3.778ThrAsp: 3.778 ± 0.357
3.674ThrGlu: 3.674 ± 0.318
2.738ThrPhe: 2.738 ± 0.303
5.581ThrGly: 5.581 ± 0.472
1.005ThrHis: 1.005 ± 0.19
4.714ThrIle: 4.714 ± 0.395
2.912ThrLys: 2.912 ± 0.32
5.442ThrLeu: 5.442 ± 0.437
1.629ThrMet: 1.629 ± 0.252
2.496ThrAsn: 2.496 ± 0.278
2.981ThrPro: 2.981 ± 0.333
2.634ThrGln: 2.634 ± 0.289
2.669ThrArg: 2.669 ± 0.312
3.813ThrSer: 3.813 ± 0.429
5.407ThrThr: 5.407 ± 0.566
5.165ThrVal: 5.165 ± 0.476
0.728ThrTrp: 0.728 ± 0.155
2.08ThrTyr: 2.08 ± 0.256
0.0ThrXaa: 0.0 ± 0.0
Val
6.239ValAla: 6.239 ± 0.486
1.144ValCys: 1.144 ± 0.183
4.402ValAsp: 4.402 ± 0.392
5.373ValGlu: 5.373 ± 0.508
3.258ValPhe: 3.258 ± 0.352
4.783ValGly: 4.783 ± 0.387
1.179ValHis: 1.179 ± 0.186
3.709ValIle: 3.709 ± 0.374
4.367ValLys: 4.367 ± 0.468
4.575ValLeu: 4.575 ± 0.405
2.357ValMet: 2.357 ± 0.3
2.6ValAsn: 2.6 ± 0.358
2.704ValPro: 2.704 ± 0.269
2.808ValGln: 2.808 ± 0.283
3.362ValArg: 3.362 ± 0.323
3.986ValSer: 3.986 ± 0.415
5.165ValThr: 5.165 ± 0.436
5.407ValVal: 5.407 ± 0.498
1.179ValTrp: 1.179 ± 0.18
2.704ValTyr: 2.704 ± 0.296
0.0ValXaa: 0.0 ± 0.0
Trp
1.213TrpAla: 1.213 ± 0.197
0.069TrpCys: 0.069 ± 0.046
0.832TrpAsp: 0.832 ± 0.141
1.282TrpGlu: 1.282 ± 0.241
0.555TrpPhe: 0.555 ± 0.124
0.867TrpGly: 0.867 ± 0.206
0.485TrpHis: 0.485 ± 0.13
0.763TrpIle: 0.763 ± 0.162
1.109TrpLys: 1.109 ± 0.213
1.525TrpLeu: 1.525 ± 0.255
0.173TrpMet: 0.173 ± 0.085
0.832TrpAsn: 0.832 ± 0.176
0.381TrpPro: 0.381 ± 0.12
0.277TrpGln: 0.277 ± 0.1
0.381TrpArg: 0.381 ± 0.12
0.659TrpSer: 0.659 ± 0.154
0.832TrpThr: 0.832 ± 0.191
0.971TrpVal: 0.971 ± 0.201
0.243TrpTrp: 0.243 ± 0.081
0.659TrpTyr: 0.659 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.704TyrAla: 2.704 ± 0.346
0.624TyrCys: 0.624 ± 0.138
2.773TyrAsp: 2.773 ± 0.348
2.253TyrGlu: 2.253 ± 0.358
1.352TyrPhe: 1.352 ± 0.2
2.912TyrGly: 2.912 ± 0.363
0.797TyrHis: 0.797 ± 0.181
1.906TyrIle: 1.906 ± 0.267
2.218TyrLys: 2.218 ± 0.287
2.392TyrLeu: 2.392 ± 0.296
1.04TyrMet: 1.04 ± 0.198
2.218TyrAsn: 2.218 ± 0.309
1.248TyrPro: 1.248 ± 0.198
1.525TyrGln: 1.525 ± 0.26
2.288TyrArg: 2.288 ± 0.241
2.218TyrSer: 2.218 ± 0.285
3.154TyrThr: 3.154 ± 0.334
2.01TyrVal: 2.01 ± 0.282
0.312TyrTrp: 0.312 ± 0.095
1.213TyrTyr: 1.213 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 171 proteins (28851 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski