Enterobacteria phage cdtI
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A5LH35|A5LH35_9CAUD Putative major tail subunit OS=Enterobacteria phage cdtI OX=414970 PE=4 SV=1
MM1 pKa = 8.0 ADD3 pKa = 3.13 PMNRR7 pKa = 11.84 HH8 pKa = 3.81 TQIRR12 pKa = 11.84 QVVLARR18 pKa = 11.84 LRR20 pKa = 11.84 EE21 pKa = 4.03 QCGDD25 pKa = 3.08 SATFFDD31 pKa = 4.77 GLPAFVDD38 pKa = 3.93 AQEE41 pKa = 4.51 LPAVSVWLSDD51 pKa = 3.26 AQYY54 pKa = 8.79 TGKK57 pKa = 7.91 MTDD60 pKa = 3.19 EE61 pKa = 5.38 DD62 pKa = 3.58 DD63 pKa = 3.67 WQAVLHH69 pKa = 5.99 IAVFIRR75 pKa = 11.84 AQAPDD80 pKa = 3.93 SEE82 pKa = 5.25 LDD84 pKa = 2.98 MWMEE88 pKa = 4.06 STIFPALNDD97 pKa = 3.5 VPALSGLIDD106 pKa = 3.52 TLIPLGFNYY115 pKa = 9.81 QRR117 pKa = 11.84 DD118 pKa = 3.81 NEE120 pKa = 4.26 MATWAMAEE128 pKa = 3.65 ITYY131 pKa = 10.47 QITYY135 pKa = 9.38 TNN137 pKa = 3.43
Molecular weight: 15.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.155
IPC2_protein 3.999
IPC_protein 3.961
Toseland 3.757
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.897
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.075
DTASelect 4.317
Thurlkill 3.808
EMBOSS 3.91
Sillero 4.088
Patrickios 3.427
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 4.003
Protein with the highest isoelectric point:
>tr|A5LH47|A5LH47_9CAUD Phage_fiber_C domain-containing protein OS=Enterobacteria phage cdtI OX=414970 PE=4 SV=1
MM1 pKa = 7.73 PFSSDD6 pKa = 2.76 SRR8 pKa = 11.84 MSAGPSRR15 pKa = 11.84 SCAVSDD21 pKa = 4.36 CPFSQSSCFPQPHH34 pKa = 6.64 RR35 pKa = 11.84 LCISFLKK42 pKa = 10.51 IFMSFSTTACITAHH56 pKa = 7.17 FIQSLNILPWPVTKK70 pKa = 10.16 PDD72 pKa = 3.38 LQMLKK77 pKa = 8.87 KK78 pKa = 8.92 TLNLCGIFGVKK89 pKa = 9.99 KK90 pKa = 9.89 GQSGDD95 pKa = 3.12 EE96 pKa = 3.87 CAICIRR102 pKa = 11.84 EE103 pKa = 3.91 PAIAVCLNNARR114 pKa = 11.84 RR115 pKa = 11.84 PQTKK119 pKa = 9.4 TDD121 pKa = 3.18 LCLPRR126 pKa = 11.84 VALWSLRR133 pKa = 11.84 SGCPHH138 pKa = 6.71 RR139 pKa = 11.84 ASRR142 pKa = 11.84 TCFSFRR148 pKa = 11.84 SLWACRR154 pKa = 11.84 TCRR157 pKa = 11.84 TGISLWTLWTCISRR171 pKa = 11.84 QTGLSPLSPVAFRR184 pKa = 11.84 PLRR187 pKa = 11.84 ACRR190 pKa = 11.84 TSITCRR196 pKa = 11.84 SPLTGCPDD204 pKa = 3.29 RR205 pKa = 11.84 TGVATLSFISLRR217 pKa = 11.84 PLRR220 pKa = 11.84 TRR222 pKa = 11.84 GPRR225 pKa = 11.84 FSLWPGRR232 pKa = 11.84 SHH234 pKa = 7.34 HH235 pKa = 6.81 GGDD238 pKa = 2.93 SVYY241 pKa = 10.83 GVFRR245 pKa = 11.84 RR246 pKa = 11.84 YY247 pKa = 8.8 PALFRR252 pKa = 4.96
Molecular weight: 28.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.336
IPC2_protein 9.311
IPC_protein 9.78
Toseland 10.452
ProMoST 10.335
Dawson 10.555
Bjellqvist 10.35
Wikipedia 10.73
Rodwell 10.687
Grimsley 10.57
Solomon 10.672
Lehninger 10.643
Nozaki 10.613
DTASelect 10.262
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.423
IPC_peptide 10.672
IPC2_peptide 10.058
IPC2.peptide.svr19 8.505
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
60
0
60
14607
59
1165
243.4
27.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.153 ± 0.558
1.438 ± 0.223
5.744 ± 0.21
5.771 ± 0.279
3.574 ± 0.267
7.298 ± 0.398
1.842 ± 0.182
5.47 ± 0.397
5.292 ± 0.283
8.352 ± 0.357
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.144
4.457 ± 0.278
4.505 ± 0.303
4.176 ± 0.479
6.278 ± 0.354
7.038 ± 0.325
5.778 ± 0.404
6.723 ± 0.367
1.753 ± 0.177
2.834 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here