Enterobacteria phage M13 (Bacteriophage M13)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Inovirus

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P69538|G9P_BPM13 Tail virion protein G9P OS=Enterobacteria phage M13 OX=1977402 GN=IX PE=1 SV=1
MM1 pKa = 7.07EE2 pKa = 4.16QVADD6 pKa = 4.42FDD8 pKa = 4.64TIYY11 pKa = 10.92QAMIQISVVLCFALGIIAGGQRR33 pKa = 3.18

Molecular weight: 3.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P69547-2|REP-2_BPM13 Isoform of P69547 Isoform G10P of Replication-associated protein G2P OS=Enterobacteria phage M13 OX=1977402 GN=II PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 9.28LTKK5 pKa = 9.8IYY7 pKa = 10.28LKK9 pKa = 10.39KK10 pKa = 10.56FSRR13 pKa = 11.84VLCLAIGFASAFTYY27 pKa = 10.44SYY29 pKa = 9.01ITQPKK34 pKa = 8.51PEE36 pKa = 4.15VKK38 pKa = 10.17KK39 pKa = 11.04VVSQTYY45 pKa = 10.61DD46 pKa = 3.05FDD48 pKa = 4.36KK49 pKa = 10.7FTIDD53 pKa = 3.03SSQRR57 pKa = 11.84LNLSYY62 pKa = 10.63RR63 pKa = 11.84YY64 pKa = 9.78VFKK67 pKa = 10.71DD68 pKa = 3.41SKK70 pKa = 11.38GKK72 pKa = 10.47LINSDD77 pKa = 4.1DD78 pKa = 4.08LQKK81 pKa = 10.71QGYY84 pKa = 8.1SLTYY88 pKa = 9.83IDD90 pKa = 5.34LCTVSIKK97 pKa = 10.46KK98 pKa = 10.48GNSNEE103 pKa = 3.96IVKK106 pKa = 10.79CNN108 pKa = 3.35

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

2

11

2164

32

426

196.7

21.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.007 ± 0.855

1.34 ± 0.308

5.638 ± 0.34

3.466 ± 0.589

5.545 ± 0.327

7.81 ± 1.67

0.878 ± 0.304

5.684 ± 0.669

5.915 ± 0.975

9.889 ± 0.99

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.756 ± 0.275

5.591 ± 0.675

4.344 ± 0.429

3.974 ± 0.36

3.651 ± 0.502

10.166 ± 0.825

5.545 ± 0.249

7.625 ± 0.776

0.924 ± 0.198

4.251 ± 0.672
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski