Amino acid dipepetide frequency for Flavobacterium subsaxonicum WB 4.1-42 = DSM 21790

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.77AlaAla: 6.77 ± 0.108	0.662AlaCys: 0.662 ± 0.028	4.61AlaAsp: 4.61 ± 0.062	4.666AlaGlu: 4.666 ± 0.07	3.685AlaPhe: 3.685 ± 0.062	5.521AlaGly: 5.521 ± 0.082	1.189AlaHis: 1.189 ± 0.038	5.642AlaIle: 5.642 ± 0.093	4.958AlaLys: 4.958 ± 0.081	7.486AlaLeu: 7.486 ± 0.088	1.733AlaMet: 1.733 ± 0.043	4.136AlaAsn: 4.136 ± 0.073	2.527AlaPro: 2.527 ± 0.057	3.299AlaGln: 3.299 ± 0.05	2.273AlaArg: 2.273 ± 0.044	4.424AlaSer: 4.424 ± 0.071	5.381AlaThr: 5.381 ± 0.115	5.312AlaVal: 5.312 ± 0.075	0.774AlaTrp: 0.774 ± 0.025	2.983AlaTyr: 2.983 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	0.522CysAla: 0.522 ± 0.022	0.098CysCys: 0.098 ± 0.008	0.439CysAsp: 0.439 ± 0.026	0.417CysGlu: 0.417 ± 0.023	0.383CysPhe: 0.383 ± 0.018	0.686CysGly: 0.686 ± 0.031	0.167CysHis: 0.167 ± 0.013	0.61CysIle: 0.61 ± 0.025	0.404CysLys: 0.404 ± 0.015	0.711CysLeu: 0.711 ± 0.022	0.16CysMet: 0.16 ± 0.012	0.433CysAsn: 0.433 ± 0.022	0.307CysPro: 0.307 ± 0.019	0.222CysGln: 0.222 ± 0.014	0.255CysArg: 0.255 ± 0.012	0.611CysSer: 0.611 ± 0.025	0.581CysThr: 0.581 ± 0.035	0.439CysVal: 0.439 ± 0.02	0.058CysTrp: 0.058 ± 0.007	0.328CysTyr: 0.328 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.68AspAla: 4.68 ± 0.065	0.409AspCys: 0.409 ± 0.021	2.979AspAsp: 2.979 ± 0.06	3.387AspGlu: 3.387 ± 0.056	3.328AspPhe: 3.328 ± 0.054	4.01AspGly: 4.01 ± 0.07	0.84AspHis: 0.84 ± 0.026	4.195AspIle: 4.195 ± 0.054	4.116AspLys: 4.116 ± 0.065	4.705AspLeu: 4.705 ± 0.062	1.177AspMet: 1.177 ± 0.032	3.303AspAsn: 3.303 ± 0.057	1.719AspPro: 1.719 ± 0.043	1.309AspGln: 1.309 ± 0.034	1.849AspArg: 1.849 ± 0.037	2.884AspSer: 2.884 ± 0.051	3.189AspThr: 3.189 ± 0.05	3.858AspVal: 3.858 ± 0.054	0.705AspTrp: 0.705 ± 0.028	2.82AspTyr: 2.82 ± 0.047	0.0AspXaa: 0.0 ± 0.0
Glu	4.78GluAla: 4.78 ± 0.07	0.344GluCys: 0.344 ± 0.02	3.049GluAsp: 3.049 ± 0.051	3.841GluGlu: 3.841 ± 0.068	2.634GluPhe: 2.634 ± 0.05	3.766GluGly: 3.766 ± 0.062	1.094GluHis: 1.094 ± 0.033	4.335GluIle: 4.335 ± 0.069	4.699GluLys: 4.699 ± 0.072	5.461GluLeu: 5.461 ± 0.073	1.48GluMet: 1.48 ± 0.039	3.506GluAsn: 3.506 ± 0.058	1.724GluPro: 1.724 ± 0.037	2.231GluGln: 2.231 ± 0.05	2.32GluArg: 2.32 ± 0.048	2.885GluSer: 2.885 ± 0.057	3.211GluThr: 3.211 ± 0.048	4.036GluVal: 4.036 ± 0.057	0.641GluTrp: 0.641 ± 0.024	2.367GluTyr: 2.367 ± 0.05	0.0GluXaa: 0.0 ± 0.0
Phe	3.615PheAla: 3.615 ± 0.054	0.498PheCys: 0.498 ± 0.02	3.13PheAsp: 3.13 ± 0.052	3.064PheGlu: 3.064 ± 0.048	2.493PhePhe: 2.493 ± 0.054	3.506PheGly: 3.506 ± 0.062	0.7PheHis: 0.7 ± 0.023	3.62PheIle: 3.62 ± 0.063	3.421PheLys: 3.421 ± 0.058	4.126PheLeu: 4.126 ± 0.064	1.122PheMet: 1.122 ± 0.033	3.071PheAsn: 3.071 ± 0.063	1.55PhePro: 1.55 ± 0.033	1.169PheGln: 1.169 ± 0.033	1.582PheArg: 1.582 ± 0.038	3.552PheSer: 3.552 ± 0.057	3.807PheThr: 3.807 ± 0.063	3.005PheVal: 3.005 ± 0.058	0.525PheTrp: 0.525 ± 0.021	2.232PheTyr: 2.232 ± 0.043	0.0PheXaa: 0.0 ± 0.0
Gly	4.815GlyAla: 4.815 ± 0.076	0.724GlyCys: 0.724 ± 0.034	3.319GlyAsp: 3.319 ± 0.051	3.399GlyGlu: 3.399 ± 0.055	3.67GlyPhe: 3.67 ± 0.06	4.844GlyGly: 4.844 ± 0.083	1.22GlyHis: 1.22 ± 0.031	5.143GlyIle: 5.143 ± 0.06	4.903GlyLys: 4.903 ± 0.069	6.075GlyLeu: 6.075 ± 0.084	1.66GlyMet: 1.66 ± 0.042	3.884GlyAsn: 3.884 ± 0.076	1.525GlyPro: 1.525 ± 0.039	2.273GlyGln: 2.273 ± 0.051	2.252GlyArg: 2.252 ± 0.045	4.33GlySer: 4.33 ± 0.064	5.078GlyThr: 5.078 ± 0.112	4.366GlyVal: 4.366 ± 0.072	0.862GlyTrp: 0.862 ± 0.029	3.143GlyTyr: 3.143 ± 0.056	0.0GlyXaa: 0.0 ± 0.0
His	1.13HisAla: 1.13 ± 0.037	0.146HisCys: 0.146 ± 0.011	0.946HisAsp: 0.946 ± 0.024	0.926HisGlu: 0.926 ± 0.029	1.09HisPhe: 1.09 ± 0.034	1.086HisGly: 1.086 ± 0.034	0.463HisHis: 0.463 ± 0.021	1.321HisIle: 1.321 ± 0.037	1.1HisLys: 1.1 ± 0.029	1.66HisLeu: 1.66 ± 0.038	0.327HisMet: 0.327 ± 0.016	1.068HisAsn: 1.068 ± 0.029	0.845HisPro: 0.845 ± 0.027	0.605HisGln: 0.605 ± 0.023	0.599HisArg: 0.599 ± 0.021	1.014HisSer: 1.014 ± 0.03	1.009HisThr: 1.009 ± 0.027	0.935HisVal: 0.935 ± 0.029	0.199HisTrp: 0.199 ± 0.013	0.916HisTyr: 0.916 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	6.131IleAla: 6.131 ± 0.081	0.557IleCys: 0.557 ± 0.018	4.373IleAsp: 4.373 ± 0.068	4.498IleGlu: 4.498 ± 0.068	3.103IlePhe: 3.103 ± 0.053	4.567IleGly: 4.567 ± 0.06	1.108IleHis: 1.108 ± 0.029	5.42IleIle: 5.42 ± 0.077	5.202IleLys: 5.202 ± 0.068	5.961IleLeu: 5.961 ± 0.089	1.4IleMet: 1.4 ± 0.037	4.264IleAsn: 4.264 ± 0.064	2.948IlePro: 2.948 ± 0.054	2.255IleGln: 2.255 ± 0.042	2.343IleArg: 2.343 ± 0.045	4.58IleSer: 4.58 ± 0.07	5.389IleThr: 5.389 ± 0.073	4.611IleVal: 4.611 ± 0.072	0.605IleTrp: 0.605 ± 0.026	2.752IleTyr: 2.752 ± 0.048	0.0IleXaa: 0.0 ± 0.0
Lys	5.262LysAla: 5.262 ± 0.083	0.314LysCys: 0.314 ± 0.017	4.32LysAsp: 4.32 ± 0.068	4.93LysGlu: 4.93 ± 0.067	2.75LysPhe: 2.75 ± 0.053	4.356LysGly: 4.356 ± 0.062	1.21LysHis: 1.21 ± 0.036	5.31LysIle: 5.31 ± 0.074	5.993LysLys: 5.993 ± 0.086	6.043LysLeu: 6.043 ± 0.086	1.921LysMet: 1.921 ± 0.039	4.456LysAsn: 4.456 ± 0.056	2.56LysPro: 2.56 ± 0.051	2.738LysGln: 2.738 ± 0.047	2.382LysArg: 2.382 ± 0.05	3.732LysSer: 3.732 ± 0.062	4.283LysThr: 4.283 ± 0.058	4.38LysVal: 4.38 ± 0.062	0.692LysTrp: 0.692 ± 0.024	2.868LysTyr: 2.868 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	6.754LeuAla: 6.754 ± 0.084	0.785LeuCys: 0.785 ± 0.025	4.81LeuAsp: 4.81 ± 0.074	5.283LeuGlu: 5.283 ± 0.078	4.626LeuPhe: 4.626 ± 0.078	5.764LeuGly: 5.764 ± 0.079	1.678LeuHis: 1.678 ± 0.038	5.834LeuIle: 5.834 ± 0.087	6.744LeuLys: 6.744 ± 0.085	9.399LeuLeu: 9.399 ± 0.117	2.024LeuMet: 2.024 ± 0.045	5.13LeuAsn: 5.13 ± 0.07	3.964LeuPro: 3.964 ± 0.06	3.633LeuGln: 3.633 ± 0.059	3.109LeuArg: 3.109 ± 0.061	6.221LeuSer: 6.221 ± 0.074	5.78LeuThr: 5.78 ± 0.077	5.491LeuVal: 5.491 ± 0.069	0.865LeuTrp: 0.865 ± 0.032	3.531LeuTyr: 3.531 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	1.944MetAla: 1.944 ± 0.038	0.132MetCys: 0.132 ± 0.01	1.2MetAsp: 1.2 ± 0.032	1.408MetGlu: 1.408 ± 0.035	0.901MetPhe: 0.901 ± 0.029	1.487MetGly: 1.487 ± 0.035	0.479MetHis: 0.479 ± 0.022	1.307MetIle: 1.307 ± 0.031	2.022MetLys: 2.022 ± 0.046	2.082MetLeu: 2.082 ± 0.042	0.555MetMet: 0.555 ± 0.022	1.034MetAsn: 1.034 ± 0.03	1.017MetPro: 1.017 ± 0.027	0.95MetGln: 0.95 ± 0.026	0.875MetArg: 0.875 ± 0.029	1.251MetSer: 1.251 ± 0.03	1.062MetThr: 1.062 ± 0.029	1.472MetVal: 1.472 ± 0.031	0.2MetTrp: 0.2 ± 0.013	0.785MetTyr: 0.785 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	4.364AsnAla: 4.364 ± 0.074	0.447AsnCys: 0.447 ± 0.022	3.035AsnAsp: 3.035 ± 0.052	3.031AsnGlu: 3.031 ± 0.043	2.924AsnPhe: 2.924 ± 0.052	4.417AsnGly: 4.417 ± 0.103	0.958AsnHis: 0.958 ± 0.025	4.306AsnIle: 4.306 ± 0.064	3.763AsnLys: 3.763 ± 0.063	5.033AsnLeu: 5.033 ± 0.073	1.197AsnMet: 1.197 ± 0.029	3.88AsnAsn: 3.88 ± 0.072	2.827AsnPro: 2.827 ± 0.051	1.889AsnGln: 1.889 ± 0.042	2.071AsnArg: 2.071 ± 0.039	3.435AsnSer: 3.435 ± 0.064	3.813AsnThr: 3.813 ± 0.074	3.718AsnVal: 3.718 ± 0.06	0.713AsnTrp: 0.713 ± 0.029	2.902AsnTyr: 2.902 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	3.079ProAla: 3.079 ± 0.06	0.206ProCys: 0.206 ± 0.014	2.376ProAsp: 2.376 ± 0.044	2.722ProGlu: 2.722 ± 0.057	1.879ProPhe: 1.879 ± 0.041	2.461ProGly: 2.461 ± 0.053	0.635ProHis: 0.635 ± 0.025	2.344ProIle: 2.344 ± 0.044	2.282ProLys: 2.282 ± 0.047	3.226ProLeu: 3.226 ± 0.05	0.742ProMet: 0.742 ± 0.025	2.17ProAsn: 2.17 ± 0.055	0.961ProPro: 0.961 ± 0.037	1.531ProGln: 1.531 ± 0.042	0.914ProArg: 0.914 ± 0.027	1.959ProSer: 1.959 ± 0.044	2.2ProThr: 2.2 ± 0.057	3.019ProVal: 3.019 ± 0.05	0.354ProTrp: 0.354 ± 0.017	1.585ProTyr: 1.585 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.595GlnAla: 2.595 ± 0.053	0.214GlnCys: 0.214 ± 0.012	1.787GlnAsp: 1.787 ± 0.04	2.132GlnGlu: 2.132 ± 0.041	1.63GlnPhe: 1.63 ± 0.036	2.009GlnGly: 2.009 ± 0.043	0.697GlnHis: 0.697 ± 0.026	2.338GlnIle: 2.338 ± 0.048	2.715GlnLys: 2.715 ± 0.044	3.56GlnLeu: 3.56 ± 0.064	0.846GlnMet: 0.846 ± 0.026	2.21GlnAsn: 2.21 ± 0.049	1.422GlnPro: 1.422 ± 0.037	1.887GlnGln: 1.887 ± 0.041	1.266GlnArg: 1.266 ± 0.03	2.016GlnSer: 2.016 ± 0.042	2.165GlnThr: 2.165 ± 0.054	2.199GlnVal: 2.199 ± 0.044	0.431GlnTrp: 0.431 ± 0.02	1.619GlnTyr: 1.619 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	2.171ArgAla: 2.171 ± 0.049	0.204ArgCys: 0.204 ± 0.012	1.794ArgAsp: 1.794 ± 0.037	2.167ArgGlu: 2.167 ± 0.048	1.818ArgPhe: 1.818 ± 0.035	1.81ArgGly: 1.81 ± 0.044	0.655ArgHis: 0.655 ± 0.024	2.675ArgIle: 2.675 ± 0.046	2.53ArgLys: 2.53 ± 0.049	3.254ArgLeu: 3.254 ± 0.054	0.911ArgMet: 0.911 ± 0.027	1.954ArgAsn: 1.954 ± 0.034	1.13ArgPro: 1.13 ± 0.034	1.289ArgGln: 1.289 ± 0.031	1.322ArgArg: 1.322 ± 0.034	1.781ArgSer: 1.781 ± 0.04	1.754ArgThr: 1.754 ± 0.041	2.211ArgVal: 2.211 ± 0.047	0.377ArgTrp: 0.377 ± 0.018	1.549ArgTyr: 1.549 ± 0.035	0.0ArgXaa: 0.0 ± 0.0
Ser	4.611SerAla: 4.611 ± 0.067	0.608SerCys: 0.608 ± 0.021	3.027SerAsp: 3.027 ± 0.044	2.987SerGlu: 2.987 ± 0.048	3.403SerPhe: 3.403 ± 0.055	4.711SerGly: 4.711 ± 0.067	1.133SerHis: 1.133 ± 0.029	4.381SerIle: 4.381 ± 0.07	3.755SerLys: 3.755 ± 0.056	5.655SerLeu: 5.655 ± 0.073	1.177SerMet: 1.177 ± 0.031	3.268SerAsn: 3.268 ± 0.06	2.254SerPro: 2.254 ± 0.046	2.061SerGln: 2.061 ± 0.049	2.067SerArg: 2.067 ± 0.043	3.479SerSer: 3.479 ± 0.06	3.562SerThr: 3.562 ± 0.07	4.104SerVal: 4.104 ± 0.059	0.628SerTrp: 0.628 ± 0.024	2.686SerTyr: 2.686 ± 0.045	0.0SerXaa: 0.0 ± 0.0
Thr	5.617ThrAla: 5.617 ± 0.119	0.416ThrCys: 0.416 ± 0.021	3.899ThrAsp: 3.899 ± 0.056	3.432ThrGlu: 3.432 ± 0.061	3.179ThrPhe: 3.179 ± 0.05	5.253ThrGly: 5.253 ± 0.101	1.055ThrHis: 1.055 ± 0.031	4.921ThrIle: 4.921 ± 0.074	3.585ThrLys: 3.585 ± 0.052	5.93ThrLeu: 5.93 ± 0.086	1.077ThrMet: 1.077 ± 0.029	3.462ThrAsn: 3.462 ± 0.078	2.94ThrPro: 2.94 ± 0.058	2.251ThrGln: 2.251 ± 0.049	1.766ThrArg: 1.766 ± 0.041	3.713ThrSer: 3.713 ± 0.066	4.842ThrThr: 4.842 ± 0.127	5.027ThrVal: 5.027 ± 0.096	0.646ThrTrp: 0.646 ± 0.026	2.748ThrTyr: 2.748 ± 0.063	0.0ThrXaa: 0.0 ± 0.0
Val	5.489ValAla: 5.489 ± 0.074	0.595ValCys: 0.595 ± 0.023	3.323ValAsp: 3.323 ± 0.05	3.369ValGlu: 3.369 ± 0.055	3.306ValPhe: 3.306 ± 0.051	3.87ValGly: 3.87 ± 0.065	1.044ValHis: 1.044 ± 0.029	4.836ValIle: 4.836 ± 0.071	4.588ValLys: 4.588 ± 0.067	6.185ValLeu: 6.185 ± 0.079	1.493ValMet: 1.493 ± 0.036	3.91ValAsn: 3.91 ± 0.063	2.507ValPro: 2.507 ± 0.047	2.108ValGln: 2.108 ± 0.043	2.101ValArg: 2.101 ± 0.048	4.291ValSer: 4.291 ± 0.07	4.959ValThr: 4.959 ± 0.091	4.776ValVal: 4.776 ± 0.069	0.628ValTrp: 0.628 ± 0.024	2.639ValTyr: 2.639 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.757TrpAla: 0.757 ± 0.029	0.098TrpCys: 0.098 ± 0.009	0.647TrpAsp: 0.647 ± 0.022	0.613TrpGlu: 0.613 ± 0.025	0.538TrpPhe: 0.538 ± 0.021	0.697TrpGly: 0.697 ± 0.027	0.254TrpHis: 0.254 ± 0.014	0.678TrpIle: 0.678 ± 0.023	0.728TrpLys: 0.728 ± 0.023	0.973TrpLeu: 0.973 ± 0.029	0.307TrpMet: 0.307 ± 0.017	0.649TrpAsn: 0.649 ± 0.022	0.298TrpPro: 0.298 ± 0.015	0.508TrpGln: 0.508 ± 0.019	0.374TrpArg: 0.374 ± 0.019	0.593TrpSer: 0.593 ± 0.024	0.557TrpThr: 0.557 ± 0.023	0.665TrpVal: 0.665 ± 0.024	0.155TrpTrp: 0.155 ± 0.011	0.465TrpTyr: 0.465 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.946TyrAla: 2.946 ± 0.05	0.376TyrCys: 0.376 ± 0.016	2.398TyrAsp: 2.398 ± 0.046	2.13TyrGlu: 2.13 ± 0.045	2.45TyrPhe: 2.45 ± 0.049	2.666TyrGly: 2.666 ± 0.045	0.81TyrHis: 0.81 ± 0.026	2.849TyrIle: 2.849 ± 0.054	2.991TyrLys: 2.991 ± 0.049	3.898TyrLeu: 3.898 ± 0.064	0.853TyrMet: 0.853 ± 0.027	2.934TyrAsn: 2.934 ± 0.056	1.619TyrPro: 1.619 ± 0.041	1.527TyrGln: 1.527 ± 0.039	1.634TyrArg: 1.634 ± 0.035	2.806TyrSer: 2.806 ± 0.049	3.138TyrThr: 3.138 ± 0.066	2.393TyrVal: 2.393 ± 0.048	0.498TyrTrp: 0.498 ± 0.022	2.196TyrTyr: 2.196 ± 0.057	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3785 proteins (1272052 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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