Fig cryptic virus
Average proteome isoelectric point is 6.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4MST4|F4MST4_9VIRU Putative capsid protein OS=Fig cryptic virus OX=882768 GN=CP PE=4 SV=1
MM1 pKa = 7.41 EE2 pKa = 6.01 AGLIEE7 pKa = 4.58 IGNIPEE13 pKa = 3.69 RR14 pKa = 11.84 HH15 pKa = 6.05 LRR17 pKa = 11.84 DD18 pKa = 3.31 EE19 pKa = 4.91 FIILVDD25 pKa = 3.62 QPAYY29 pKa = 10.87 DD30 pKa = 3.58 SVRR33 pKa = 11.84 RR34 pKa = 11.84 NAPQADD40 pKa = 3.77 MQEE43 pKa = 3.77 IDD45 pKa = 2.52 GWARR49 pKa = 11.84 SFYY52 pKa = 9.43 TVEE55 pKa = 4.96 GIMASIMQFSKK66 pKa = 10.85 PLIHH70 pKa = 7.13 EE71 pKa = 4.31 PTDD74 pKa = 4.59 PIWNDD79 pKa = 3.2 VKK81 pKa = 11.05 RR82 pKa = 11.84 EE83 pKa = 3.86 TLMKK87 pKa = 10.06 IGSLFPQVQSLPFEE101 pKa = 4.61 GGFDD105 pKa = 3.44 HH106 pKa = 7.42 VPFEE110 pKa = 4.53 SSSSAGYY117 pKa = 10.46 GYY119 pKa = 10.37 DD120 pKa = 3.4 GKK122 pKa = 10.94 KK123 pKa = 10.68 GEE125 pKa = 4.42 GNNFHH130 pKa = 6.9 RR131 pKa = 11.84 AKK133 pKa = 10.48 SIANAAVRR141 pKa = 11.84 KK142 pKa = 9.46 FSEE145 pKa = 5.34 DD146 pKa = 2.92 IDD148 pKa = 4.0 NQGYY152 pKa = 10.12 DD153 pKa = 3.49 YY154 pKa = 11.34 AVSHH158 pKa = 7.41 LIQQGTPDD166 pKa = 3.07 IAFTRR171 pKa = 11.84 TQLAKK176 pKa = 10.59 LPSIKK181 pKa = 10.16 VRR183 pKa = 11.84 IVFGEE188 pKa = 3.94 AFHH191 pKa = 6.98 NILIEE196 pKa = 4.29 GLSAAPLLEE205 pKa = 4.0 AFKK208 pKa = 11.16 RR209 pKa = 11.84 MDD211 pKa = 3.07 TFYY214 pKa = 10.3 FTGKK218 pKa = 10.29 DD219 pKa = 3.08 PTIYY223 pKa = 10.39 VPRR226 pKa = 11.84 ILHH229 pKa = 6.16 KK230 pKa = 10.44 MSTNEE235 pKa = 3.37 GWFICLDD242 pKa = 3.51 WKK244 pKa = 11.31 AFDD247 pKa = 4.86 ASVQLWEE254 pKa = 4.4 IDD256 pKa = 3.43 HH257 pKa = 6.94 AFNCIQQLLAFPTEE271 pKa = 4.26 LSRR274 pKa = 11.84 LAFLFTRR281 pKa = 11.84 EE282 pKa = 3.97 SFKK285 pKa = 10.55 QRR287 pKa = 11.84 KK288 pKa = 8.16 LADD291 pKa = 3.76 PNGILWMRR299 pKa = 11.84 KK300 pKa = 8.66 GGIPSGSYY308 pKa = 6.73 YY309 pKa = 10.66 TNIIGSVINYY319 pKa = 8.88 NRR321 pKa = 11.84 IEE323 pKa = 4.26 YY324 pKa = 8.74 VCKK327 pKa = 10.45 RR328 pKa = 11.84 LGLQKK333 pKa = 9.64 TSCYY337 pKa = 10.02 VQGDD341 pKa = 3.77 DD342 pKa = 4.79 SLIHH346 pKa = 5.7 ITGDD350 pKa = 3.28 AKK352 pKa = 10.85 PDD354 pKa = 3.48 LTQLQMLGEE363 pKa = 4.15 QFGWTLNIPKK373 pKa = 10.28 CSLTQDD379 pKa = 3.44 SQLVTFLGRR388 pKa = 11.84 SQMHH392 pKa = 5.43 QLNIRR397 pKa = 11.84 EE398 pKa = 4.05 RR399 pKa = 11.84 LKK401 pKa = 10.86 VLRR404 pKa = 11.84 LMCFPEE410 pKa = 4.31 YY411 pKa = 10.24 KK412 pKa = 10.45 VEE414 pKa = 4.15 DD415 pKa = 4.08 PKK417 pKa = 11.1 ISTTRR422 pKa = 11.84 VKK424 pKa = 10.77 AIARR428 pKa = 11.84 DD429 pKa = 3.94 AGWSDD434 pKa = 3.14 PVYY437 pKa = 11.24 NKK439 pKa = 10.13 IYY441 pKa = 10.3 LQLKK445 pKa = 8.15 RR446 pKa = 11.84 LYY448 pKa = 10.91 GEE450 pKa = 4.25 VEE452 pKa = 4.08 RR453 pKa = 11.84 LPPHH457 pKa = 6.92 LATFVDD463 pKa = 4.39 RR464 pKa = 11.84 FDD466 pKa = 4.17 FQDD469 pKa = 3.14 VNMM472 pKa = 4.97
Molecular weight: 53.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.09
IPC2_protein 6.046
IPC_protein 6.148
Toseland 6.478
ProMoST 6.504
Dawson 6.453
Bjellqvist 6.44
Wikipedia 6.478
Rodwell 6.453
Grimsley 6.62
Solomon 6.465
Lehninger 6.453
Nozaki 6.722
DTASelect 6.912
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.839
Patrickios 4.279
IPC_peptide 6.478
IPC2_peptide 6.722
IPC2.peptide.svr19 6.73
Protein with the highest isoelectric point:
>tr|F4MST4|F4MST4_9VIRU Putative capsid protein OS=Fig cryptic virus OX=882768 GN=CP PE=4 SV=1
MM1 pKa = 7.75 AAPNFDD7 pKa = 3.5 ILEE10 pKa = 4.22 DD11 pKa = 4.74 LIADD15 pKa = 3.64 GRR17 pKa = 11.84 LQITPYY23 pKa = 9.12 VTSTEE28 pKa = 3.95 PKK30 pKa = 10.08 IFDD33 pKa = 3.5 VDD35 pKa = 6.02 FDD37 pKa = 4.5 ALWNRR42 pKa = 11.84 INAIIVPIYY51 pKa = 9.43 TNWDD55 pKa = 3.3 QLRR58 pKa = 11.84 FHH60 pKa = 7.04 YY61 pKa = 10.3 PGTTTTVTNNITLDD75 pKa = 3.42 VGHH78 pKa = 7.46 LVTFALWQLYY88 pKa = 9.63 NRR90 pKa = 11.84 LRR92 pKa = 11.84 SVAEE96 pKa = 4.33 CFHH99 pKa = 6.54 HH100 pKa = 5.95 PTRR103 pKa = 11.84 TSRR106 pKa = 11.84 MNTRR110 pKa = 11.84 APISNRR116 pKa = 11.84 MEE118 pKa = 4.22 FPSFLSSMLEE128 pKa = 4.08 SISWLRR134 pKa = 11.84 INDD137 pKa = 3.73 GPVDD141 pKa = 3.83 YY142 pKa = 10.54 LALFTAPTGTSNNYY156 pKa = 8.92 GRR158 pKa = 11.84 STVQVPDD165 pKa = 3.33 NGCYY169 pKa = 10.21 DD170 pKa = 3.83 RR171 pKa = 11.84 LTGKK175 pKa = 9.98 LRR177 pKa = 11.84 ALGVQLTPIDD187 pKa = 3.93 MTPSAGSFWPTTQVITEE204 pKa = 4.11 EE205 pKa = 3.92 EE206 pKa = 4.84 LYY208 pKa = 11.05 NIVGTFHH215 pKa = 6.9 SSHH218 pKa = 6.16 YY219 pKa = 9.99 VNEE222 pKa = 4.08 DD223 pKa = 3.49 AIRR226 pKa = 11.84 AIFLAGTAHH235 pKa = 6.11 SQPFPDD241 pKa = 4.19 FGMTVGFVTDD251 pKa = 3.81 RR252 pKa = 11.84 TVMDD256 pKa = 4.83 PLAALAAPTGTPRR269 pKa = 11.84 SEE271 pKa = 4.13 EE272 pKa = 3.87 ATSAGRR278 pKa = 11.84 PVHH281 pKa = 6.35 GMYY284 pKa = 10.46 NVNHH288 pKa = 7.02 RR289 pKa = 11.84 GIQPAAGEE297 pKa = 4.19 QNPSGCYY304 pKa = 9.45 IIGRR308 pKa = 11.84 GYY310 pKa = 8.98 EE311 pKa = 3.8 RR312 pKa = 11.84 RR313 pKa = 11.84 YY314 pKa = 7.61 TCYY317 pKa = 9.55 LARR320 pKa = 11.84 RR321 pKa = 11.84 IAVFEE326 pKa = 4.2 ANRR329 pKa = 11.84 IIRR332 pKa = 11.84 YY333 pKa = 9.49 RR334 pKa = 11.84 FMRR337 pKa = 4.14
Molecular weight: 37.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.135
IPC2_protein 6.084
IPC_protein 6.211
Toseland 6.576
ProMoST 6.634
Dawson 6.547
Bjellqvist 6.504
Wikipedia 6.576
Rodwell 6.547
Grimsley 6.722
Solomon 6.561
Lehninger 6.547
Nozaki 6.81
DTASelect 7.0
Thurlkill 7.029
EMBOSS 7.015
Sillero 6.942
Patrickios 3.923
IPC_peptide 6.576
IPC2_peptide 6.927
IPC2.peptide.svr19 6.781
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
809
337
472
404.5
45.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.922 ± 0.619
1.236 ± 0.028
5.81 ± 0.434
4.944 ± 0.449
5.562 ± 0.294
6.551 ± 0.013
2.472 ± 0.113
7.417 ± 0.336
3.461 ± 1.628
8.282 ± 0.659
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.042
4.574 ± 0.604
5.686 ± 0.478
4.203 ± 0.87
6.675 ± 0.759
5.933 ± 0.168
6.799 ± 1.7
5.315 ± 0.352
1.607 ± 0.07
3.956 ± 0.281
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here