Escherichia phage pro147

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Peduovirus; Escherichia virus pro147

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7LDG9|A0A0F7LDG9_9CAUD Putative zinc-finger containing protein OS=Escherichia phage pro147 OX=1649239 GN=pro147_37 PE=4 SV=1
MM1 pKa = 7.01LTSTIVSNSLCYY13 pKa = 10.49LSTVRR18 pKa = 11.84NVVGYY23 pKa = 7.73GTIVVDD29 pKa = 5.08DD30 pKa = 5.0GGASLIGPP38 pKa = 4.66

Molecular weight: 3.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7LDR8|A0A0F7LDR8_9CAUD Endolysin OS=Escherichia phage pro147 OX=1649239 GN=pro147_21 PE=3 SV=1
MM1 pKa = 7.44NEE3 pKa = 4.18FKK5 pKa = 10.81RR6 pKa = 11.84FEE8 pKa = 4.32DD9 pKa = 3.63RR10 pKa = 11.84LTGLIEE16 pKa = 4.13SLSPSGRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84LSAEE29 pKa = 3.33LAKK32 pKa = 10.46RR33 pKa = 11.84LRR35 pKa = 11.84QSQQRR40 pKa = 11.84RR41 pKa = 11.84VMAQKK46 pKa = 10.87APDD49 pKa = 3.77GTPYY53 pKa = 10.98APRR56 pKa = 11.84QQQSARR62 pKa = 11.84KK63 pKa = 7.47KK64 pKa = 7.38TGRR67 pKa = 11.84VKK69 pKa = 10.73RR70 pKa = 11.84KK71 pKa = 8.61MFAKK75 pKa = 10.7LITSRR80 pKa = 11.84FLHH83 pKa = 6.02IRR85 pKa = 11.84ASPEE89 pKa = 3.64QASMEE94 pKa = 4.49FYY96 pKa = 10.64GGKK99 pKa = 9.19SPKK102 pKa = 9.38IASVHH107 pKa = 4.38QFGLSEE113 pKa = 4.18EE114 pKa = 4.23TRR116 pKa = 11.84KK117 pKa = 10.46DD118 pKa = 3.29GKK120 pKa = 10.92KK121 pKa = 9.06IDD123 pKa = 3.86YY124 pKa = 7.69PARR127 pKa = 11.84PLLGFTGEE135 pKa = 4.09DD136 pKa = 3.01VQMIEE141 pKa = 4.58EE142 pKa = 5.0IILAHH147 pKa = 7.11LDD149 pKa = 3.34RR150 pKa = 6.82

Molecular weight: 17.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

10124

38

815

230.1

25.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.492 ± 0.764

0.968 ± 0.142

5.531 ± 0.256

6.292 ± 0.27

3.595 ± 0.286

6.381 ± 0.39

1.857 ± 0.177

5.68 ± 0.262

6.124 ± 0.439

9.058 ± 0.359

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.815 ± 0.197

4.188 ± 0.339

3.941 ± 0.237

3.734 ± 0.238

6.351 ± 0.496

6.381 ± 0.297

6.588 ± 0.354

6.648 ± 0.337

1.343 ± 0.139

3.032 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski