Beihai razor shell virus 3
Average proteome isoelectric point is 5.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KE88|A0A1L3KE88_9VIRU RNA-directed RNA polymerase OS=Beihai razor shell virus 3 OX=1922647 PE=4 SV=1
MM1 pKa = 7.7 PEE3 pKa = 3.47 GWQRR7 pKa = 11.84 PPKK10 pKa = 10.35 GKK12 pKa = 9.77 YY13 pKa = 9.91 ARR15 pKa = 11.84 DD16 pKa = 3.38 AMLGSLEE23 pKa = 3.84 WHH25 pKa = 6.77 LEE27 pKa = 3.71 QNQRR31 pKa = 11.84 ARR33 pKa = 11.84 SQCQSVPTSALSDD46 pKa = 2.94 IGGRR50 pKa = 11.84 VAATYY55 pKa = 9.96 CKK57 pKa = 9.71 QQEE60 pKa = 4.4 LCRR63 pKa = 11.84 NLCQQGCWLQSASGEE78 pKa = 4.17 PIAVRR83 pKa = 11.84 KK84 pKa = 9.71 GSRR87 pKa = 11.84 GSQRR91 pKa = 11.84 DD92 pKa = 3.76 GAQHH96 pKa = 5.52 QPVGQSFFSSEE107 pKa = 4.26 AEE109 pKa = 4.15 DD110 pKa = 4.34 GCDD113 pKa = 3.21 EE114 pKa = 5.15 GVLQFLGFTGSGQRR128 pKa = 11.84 IGPDD132 pKa = 3.0 QRR134 pKa = 11.84 FAEE137 pKa = 4.27 INGAIACEE145 pKa = 4.24 IEE147 pKa = 4.03 HH148 pKa = 6.84 HH149 pKa = 6.19 EE150 pKa = 4.26 CCKK153 pKa = 10.53 TEE155 pKa = 3.92 TEE157 pKa = 4.4 EE158 pKa = 5.62 RR159 pKa = 11.84 EE160 pKa = 4.2 CCQQVEE166 pKa = 4.31 AGRR169 pKa = 11.84 ALDD172 pKa = 3.69 TEE174 pKa = 4.49 RR175 pKa = 11.84 VTRR178 pKa = 11.84 LDD180 pKa = 3.99 DD181 pKa = 3.5 YY182 pKa = 11.85 SLGFRR187 pKa = 11.84 SLVSDD192 pKa = 4.2 VARR195 pKa = 11.84 SWGASGVDD203 pKa = 3.66 DD204 pKa = 5.22 AGVAATIDD212 pKa = 4.45 EE213 pKa = 4.37 IVATYY218 pKa = 9.57 PCLRR222 pKa = 11.84 DD223 pKa = 3.07 AAVFGRR229 pKa = 11.84 FLGSRR234 pKa = 11.84 FGNEE238 pKa = 3.12 RR239 pKa = 11.84 VLNPLSTPGVEE250 pKa = 3.64 WRR252 pKa = 11.84 LFGADD257 pKa = 3.14 NQTVFGYY264 pKa = 10.61 DD265 pKa = 3.02 AFFGEE270 pKa = 4.7 YY271 pKa = 10.22 NGVHH275 pKa = 6.1 VEE277 pKa = 4.12 DD278 pKa = 4.69 LRR280 pKa = 11.84 CAVIEE285 pKa = 4.5 KK286 pKa = 10.05 ILRR289 pKa = 11.84 LIDD292 pKa = 4.18 GPHH295 pKa = 7.37 LDD297 pKa = 4.97 RR298 pKa = 11.84 IHH300 pKa = 6.76 TFLKK304 pKa = 10.68 DD305 pKa = 3.55 EE306 pKa = 3.93 PHH308 pKa = 6.49 KK309 pKa = 10.25 PEE311 pKa = 4.12 KK312 pKa = 9.79 VAEE315 pKa = 4.24 GRR317 pKa = 11.84 WRR319 pKa = 11.84 IISGVSATDD328 pKa = 3.23 QIACHH333 pKa = 6.33 LLMGEE338 pKa = 4.3 VLSSFSATPLTSGTAIGWNVMMHH361 pKa = 6.67 GGLSSIFSLLPFGEE375 pKa = 4.25 TGKK378 pKa = 10.71 LVSADD383 pKa = 3.48 RR384 pKa = 11.84 KK385 pKa = 8.77 SWDD388 pKa = 3.0 WTVQKK393 pKa = 10.64 LAVVLFTAFALLLLDD408 pKa = 5.8 YY409 pKa = 8.66 KK410 pKa = 8.18 TQWEE414 pKa = 4.1 RR415 pKa = 11.84 KK416 pKa = 9.23 AIVNHH421 pKa = 6.04 ICSIFAIRR429 pKa = 11.84 RR430 pKa = 11.84 FDD432 pKa = 3.39 CQGRR436 pKa = 11.84 VVRR439 pKa = 11.84 FDD441 pKa = 3.3 NFGVLLSGWKK451 pKa = 8.28 FTALFNSVVQHH462 pKa = 6.78 LYY464 pKa = 11.29 HH465 pKa = 6.39 MLACSMLSLNGRR477 pKa = 11.84 LPLSMGDD484 pKa = 3.39 DD485 pKa = 3.77 TVQEE489 pKa = 4.52 EE490 pKa = 4.76 MPQSYY495 pKa = 8.9 WDD497 pKa = 3.51 HH498 pKa = 6.62 LATFGVILKK507 pKa = 8.62 PTEE510 pKa = 4.01 TFDD513 pKa = 4.67 EE514 pKa = 4.83 GFEE517 pKa = 4.11 FCGAHH522 pKa = 5.92 FKK524 pKa = 11.09 VNSFSPVYY532 pKa = 10.13 LPKK535 pKa = 10.16 HH536 pKa = 6.09 AYY538 pKa = 8.96 NLRR541 pKa = 11.84 DD542 pKa = 3.58 VSPEE546 pKa = 3.81 NLPALLISYY555 pKa = 9.39 RR556 pKa = 11.84 YY557 pKa = 10.42 LYY559 pKa = 10.34 MFDD562 pKa = 3.18 EE563 pKa = 4.67 RR564 pKa = 11.84 RR565 pKa = 11.84 FNALNRR571 pKa = 11.84 WACEE575 pKa = 3.79 MGFDD579 pKa = 4.27 SLDD582 pKa = 3.43 RR583 pKa = 11.84 SEE585 pKa = 6.3 LIFKK589 pKa = 10.28 VLGVSEE595 pKa = 4.32 MSEE598 pKa = 4.17 VMVV601 pKa = 4.28
Molecular weight: 67.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.314
IPC2_protein 5.334
IPC_protein 5.334
Toseland 5.499
ProMoST 5.575
Dawson 5.461
Bjellqvist 5.537
Wikipedia 5.385
Rodwell 5.41
Grimsley 5.525
Solomon 5.461
Lehninger 5.436
Nozaki 5.652
DTASelect 5.817
Thurlkill 5.664
EMBOSS 5.614
Sillero 5.741
Patrickios 3.935
IPC_peptide 5.474
IPC2_peptide 5.741
IPC2.peptide.svr19 5.716
Protein with the highest isoelectric point:
>tr|A0A1L3KE88|A0A1L3KE88_9VIRU RNA-directed RNA polymerase OS=Beihai razor shell virus 3 OX=1922647 PE=4 SV=1
MM1 pKa = 7.37 KK2 pKa = 10.55 RR3 pKa = 11.84 LGLLLTMTFVEE14 pKa = 4.41 QTEE17 pKa = 4.55 HH18 pKa = 6.97 VFEE21 pKa = 4.23 AVRR24 pKa = 11.84 NKK26 pKa = 9.93 SRR28 pKa = 11.84 ALINTRR34 pKa = 11.84 LSEE37 pKa = 4.06 IWNMTFPEE45 pKa = 3.82 IRR47 pKa = 11.84 LVEE50 pKa = 4.25 RR51 pKa = 11.84 KK52 pKa = 8.52 PWEE55 pKa = 4.49 SVSSTGDD62 pKa = 3.16 EE63 pKa = 4.33 SVLHH67 pKa = 5.2 QFCMSACFVIIALFVLPVICCIVRR91 pKa = 11.84 SVGAVAWDD99 pKa = 3.87 HH100 pKa = 6.37 LCSFALVMVEE110 pKa = 3.77 LVKK113 pKa = 10.66 WMLRR117 pKa = 11.84 VTVAVTTVIMLVVRR131 pKa = 11.84 LVWAWWYY138 pKa = 11.11 NDD140 pKa = 4.78 LYY142 pKa = 11.41 SCMIATFLVAVVSIYY157 pKa = 10.81 LLWRR161 pKa = 11.84 LFEE164 pKa = 5.33 ADD166 pKa = 2.92 SAQIYY171 pKa = 10.69 SGFEE175 pKa = 4.06 SIKK178 pKa = 10.78 GGTNAPFRR186 pKa = 11.84 IEE188 pKa = 4.26 EE189 pKa = 4.17 LSIMPLGSSDD199 pKa = 4.42 FEE201 pKa = 4.39 ASSPSSSVVQVGFQRR216 pKa = 11.84 GFGLNRR222 pKa = 11.84 AFVTVGNAIYY232 pKa = 10.12 FRR234 pKa = 11.84 EE235 pKa = 4.34 LDD237 pKa = 3.61 GKK239 pKa = 10.2 HH240 pKa = 5.47 YY241 pKa = 10.59 FSVPTHH247 pKa = 6.63 VITALSSVTSAVPLLKK263 pKa = 10.55 RR264 pKa = 11.84 GTTVIAVEE272 pKa = 4.68 RR273 pKa = 11.84 ICTLGLQHH281 pKa = 6.91 LAEE284 pKa = 4.27 FTVLVAGQRR293 pKa = 11.84 ARR295 pKa = 11.84 GMASLARR302 pKa = 11.84 VGHH305 pKa = 5.02 VDD307 pKa = 4.33 RR308 pKa = 11.84 RR309 pKa = 11.84 DD310 pKa = 3.26 ACPVVVHH317 pKa = 6.44 AHH319 pKa = 5.9 TDD321 pKa = 3.23 SGSALSKK328 pKa = 10.78 GQLLPATGMGSSGVHH343 pKa = 5.1 VFHH346 pKa = 7.04 HH347 pKa = 6.04 TASTDD352 pKa = 3.51 KK353 pKa = 10.11 GWSGGLVTMDD363 pKa = 3.56 GKK365 pKa = 10.92 AIGQHH370 pKa = 5.37 VGWVSAKK377 pKa = 10.49 DD378 pKa = 3.77 PYY380 pKa = 10.74 HH381 pKa = 6.81 GEE383 pKa = 4.0 SKK385 pKa = 11.34 ANVAFNLAYY394 pKa = 10.56 LYY396 pKa = 10.78 AIVDD400 pKa = 3.56 WKK402 pKa = 10.78 LRR404 pKa = 11.84 PAGDD408 pKa = 3.56 RR409 pKa = 11.84 SGLEE413 pKa = 3.84 ADD415 pKa = 4.54 SFTSPLIDD423 pKa = 3.7 RR424 pKa = 11.84 LVADD428 pKa = 4.44 YY429 pKa = 7.83 EE430 pKa = 4.32 TDD432 pKa = 5.03 DD433 pKa = 4.25 RR434 pKa = 11.84 NDD436 pKa = 3.05 WYY438 pKa = 11.35 VEE440 pKa = 3.91 EE441 pKa = 4.81 LVSPNLDD448 pKa = 2.77 VTYY451 pKa = 10.51 IVFNRR456 pKa = 11.84 RR457 pKa = 11.84 SGKK460 pKa = 7.82 VSRR463 pKa = 11.84 YY464 pKa = 9.38 EE465 pKa = 4.08 RR466 pKa = 11.84 DD467 pKa = 3.53 DD468 pKa = 4.04 LSSDD472 pKa = 3.0 KK473 pKa = 11.31 SSMIRR478 pKa = 11.84 RR479 pKa = 11.84 YY480 pKa = 9.34 IAGSGTEE487 pKa = 3.76 NAEE490 pKa = 4.05 FQDD493 pKa = 4.08 ASSEE497 pKa = 4.06 MTTACEE503 pKa = 3.88 EE504 pKa = 4.42 YY505 pKa = 10.3 VDD507 pKa = 3.52 KK508 pKa = 10.72 EE509 pKa = 3.68 YY510 pKa = 11.12 RR511 pKa = 11.84 RR512 pKa = 11.84 LVRR515 pKa = 11.84 EE516 pKa = 3.6 IDD518 pKa = 3.64 EE519 pKa = 4.78 LMGSYY524 pKa = 8.79 MRR526 pKa = 11.84 NNGVDD531 pKa = 2.91 AAMDD535 pKa = 3.28 VMRR538 pKa = 11.84 EE539 pKa = 3.59 ITYY542 pKa = 9.81 LAKK545 pKa = 10.61 AQSTYY550 pKa = 10.75 IQTLCDD556 pKa = 3.52 NLRR559 pKa = 11.84 TPNRR563 pKa = 11.84 GMEE566 pKa = 4.44 ALPVVPVDD574 pKa = 3.49 YY575 pKa = 11.45 GNLRR579 pKa = 11.84 TMMVLVEE586 pKa = 4.35 QYY588 pKa = 11.79 ADD590 pKa = 3.13 IQRR593 pKa = 11.84 QLGSMAATEE602 pKa = 3.82 QLAAGEE608 pKa = 3.85 IRR610 pKa = 11.84 ARR612 pKa = 11.84 VAAGEE617 pKa = 4.22 PKK619 pKa = 10.31 EE620 pKa = 4.19 QVARR624 pKa = 11.84 DD625 pKa = 3.56 QMKK628 pKa = 10.2 IIANMRR634 pKa = 11.84 AINAAIEE641 pKa = 4.06 LCPNIGRR648 pKa = 11.84 EE649 pKa = 4.14 LTMLARR655 pKa = 11.84 NQAQLSADD663 pKa = 3.32 EE664 pKa = 4.37 VTSRR668 pKa = 11.84 NSMDD672 pKa = 3.12 RR673 pKa = 11.84 VRR675 pKa = 11.84 RR676 pKa = 11.84 KK677 pKa = 9.77 LRR679 pKa = 11.84 NEE681 pKa = 4.6 GYY683 pKa = 10.73 DD684 pKa = 3.54 PAADD688 pKa = 3.93 PATIDD693 pKa = 3.4 AVIAQRR699 pKa = 11.84 QALLNAQQAAKK710 pKa = 10.22 AIPLRR715 pKa = 11.84 PEE717 pKa = 4.05 APLVIPVAAQGPEE730 pKa = 4.25 SLTLDD735 pKa = 3.48 YY736 pKa = 10.99 YY737 pKa = 10.78 RR738 pKa = 11.84 RR739 pKa = 11.84 KK740 pKa = 9.68 SQEE743 pKa = 3.7 GTNLTGFNVYY753 pKa = 10.25 FDD755 pKa = 4.02 PLKK758 pKa = 10.61 VDD760 pKa = 3.34 EE761 pKa = 5.05 EE762 pKa = 4.3 EE763 pKa = 4.46 VIRR766 pKa = 11.84 QIRR769 pKa = 11.84 DD770 pKa = 3.0 IKK772 pKa = 10.47 QSAVSFLVPRR782 pKa = 11.84 PASSGLNSEE791 pKa = 4.17 VLKK794 pKa = 10.87 PKK796 pKa = 10.06 VAQTMPASASLSSVTQSTSAEE817 pKa = 4.17 EE818 pKa = 4.38 TTKK821 pKa = 11.01 SPTTLPNYY829 pKa = 7.55 SQCQKK834 pKa = 10.84 AGSDD838 pKa = 3.54 PRR840 pKa = 11.84 KK841 pKa = 10.12 ASMPEE846 pKa = 4.22 TQCLGALNGTLSRR859 pKa = 11.84 TNGLEE864 pKa = 4.0 ANVNQCLPVHH874 pKa = 6.01 YY875 pKa = 7.56 PTLEE879 pKa = 4.3 AEE881 pKa = 4.44 SQQLIANSKK890 pKa = 10.26 SCVEE894 pKa = 4.19 TCASKK899 pKa = 10.92 GVGCSQPQASQSPLEE914 pKa = 4.14 KK915 pKa = 10.71 VRR917 pKa = 11.84 VDD919 pKa = 3.7 LSEE922 pKa = 3.72 MARR925 pKa = 11.84 NINRR929 pKa = 11.84 LANPSSAPKK938 pKa = 10.52 LKK940 pKa = 9.68 MGVMRR945 pKa = 11.84 EE946 pKa = 4.18 FFSSLASQEE955 pKa = 3.94 VDD957 pKa = 4.25 SEE959 pKa = 4.2 LDD961 pKa = 3.47 QIKK964 pKa = 10.4 GLQKK968 pKa = 11.05 SMEE971 pKa = 4.04 QLHH974 pKa = 6.73 AKK976 pKa = 9.68 LNTMSAAKK984 pKa = 9.73 PKK986 pKa = 10.11 RR987 pKa = 11.84 RR988 pKa = 11.84 NASVVSKK995 pKa = 11.15
Molecular weight: 109.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.288
IPC2_protein 6.338
IPC_protein 6.389
Toseland 6.491
ProMoST 6.839
Dawson 6.781
Bjellqvist 6.781
Wikipedia 6.766
Rodwell 6.766
Grimsley 6.576
Solomon 6.781
Lehninger 6.781
Nozaki 7.088
DTASelect 7.176
Thurlkill 7.205
EMBOSS 7.176
Sillero 7.19
Patrickios 4.342
IPC_peptide 6.795
IPC2_peptide 6.912
IPC2.peptide.svr19 6.798
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1596
601
995
798.0
88.41
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.023 ± 0.801
2.256 ± 0.473
5.013 ± 0.336
6.454 ± 0.192
3.947 ± 0.979
6.642 ± 0.962
2.005 ± 0.343
4.261 ± 0.139
3.697 ± 0.279
9.273 ± 0.197
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.882 ± 0.375
3.509 ± 0.355
4.01 ± 0.182
4.386 ± 0.056
6.642 ± 0.007
8.584 ± 0.485
5.013 ± 0.445
8.271 ± 0.669
1.566 ± 0.311
2.569 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here