Rhodobacter maris
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3687 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A285SG54|A0A285SG54_9RHOB Myo-inositol-1(Or 4)-monophosphatase OS=Rhodobacter maris OX=446682 GN=SAMN05877831_10551 PE=4 SV=1
MM1 pKa = 6.33 TTWTIQGFSGSDD13 pKa = 3.29 LIIAEE18 pKa = 4.58 GGSTLSVGSNFMLDD32 pKa = 3.84 PTWDD36 pKa = 3.35 ASTDD40 pKa = 3.24 ATSYY44 pKa = 10.63 TFTDD48 pKa = 3.79 PDD50 pKa = 3.75 DD51 pKa = 4.25 TNLSGDD57 pKa = 3.75 NLNNEE62 pKa = 4.64 HH63 pKa = 6.9 GNDD66 pKa = 3.68 GNQTINVYY74 pKa = 10.42 DD75 pKa = 3.48 SSGTLIHH82 pKa = 6.61 SGQVYY87 pKa = 10.17 IEE89 pKa = 4.64 DD90 pKa = 3.75 SAVFTAPDD98 pKa = 3.86 GSSITLYY105 pKa = 9.16 TLEE108 pKa = 4.14 VNGVVVGTVTSAPLVPGVTYY128 pKa = 10.47 HH129 pKa = 5.02 VTAINDD135 pKa = 3.48 VTTGPLYY142 pKa = 10.16 TALASTTYY150 pKa = 11.08 DD151 pKa = 3.42 PDD153 pKa = 3.47 LANTVTGGDD162 pKa = 3.71 YY163 pKa = 11.07 ADD165 pKa = 5.22 SITGGAGNDD174 pKa = 4.08 TITTGAGSDD183 pKa = 3.88 TIDD186 pKa = 3.66 GGTGNDD192 pKa = 3.7 SIQFGTGGDD201 pKa = 3.87 SVHH204 pKa = 6.93 GGDD207 pKa = 4.03 GNDD210 pKa = 4.42 YY211 pKa = 10.44 IDD213 pKa = 5.72 DD214 pKa = 4.15 YY215 pKa = 11.62 AGSTGYY221 pKa = 10.74 VYY223 pKa = 11.24 NDD225 pKa = 3.72 TLYY228 pKa = 11.47 GDD230 pKa = 4.74 AGNDD234 pKa = 3.62 TIFAGSGNDD243 pKa = 3.77 YY244 pKa = 11.27 VDD246 pKa = 4.45 GGADD250 pKa = 3.21 NDD252 pKa = 4.06 NIYY255 pKa = 11.21 GEE257 pKa = 5.03 DD258 pKa = 4.07 GNDD261 pKa = 3.51 TLLGGAGCDD270 pKa = 3.6 WIEE273 pKa = 4.35 GGAGADD279 pKa = 4.12 SIDD282 pKa = 3.92 GGTGTDD288 pKa = 3.86 IILGGDD294 pKa = 3.75 GNDD297 pKa = 4.2 TIHH300 pKa = 6.85 GGDD303 pKa = 3.56 GADD306 pKa = 3.37 YY307 pKa = 10.94 LAGNTGNDD315 pKa = 3.35 VLYY318 pKa = 11.21 GEE320 pKa = 5.24 ADD322 pKa = 3.17 ADD324 pKa = 3.54 RR325 pKa = 11.84 FYY327 pKa = 11.43 FEE329 pKa = 5.42 NGWGSDD335 pKa = 3.21 TVYY338 pKa = 11.0 GGSTTTAGGVDD349 pKa = 5.12 LDD351 pKa = 4.4 TLDD354 pKa = 4.06 FSYY357 pKa = 9.84 DD358 pKa = 3.48 TVSGVSVTFTDD369 pKa = 3.8 WEE371 pKa = 4.95 DD372 pKa = 3.38 GTATDD377 pKa = 4.0 GTSTVTFDD385 pKa = 4.25 NIEE388 pKa = 4.51 GIYY391 pKa = 9.1 GTAFGDD397 pKa = 4.06 TINASADD404 pKa = 3.51 GSGLTLDD411 pKa = 4.2 GQAGNDD417 pKa = 3.94 SIIGGSGDD425 pKa = 3.75 DD426 pKa = 4.74 CILGGAGDD434 pKa = 4.0 DD435 pKa = 4.23 TIFSGFGNDD444 pKa = 3.01 TVYY447 pKa = 11.14 GGDD450 pKa = 3.89 GNDD453 pKa = 4.59 SIDD456 pKa = 4.07 DD457 pKa = 3.75 KK458 pKa = 11.64 SGVLAEE464 pKa = 4.52 TYY466 pKa = 10.07 TNYY469 pKa = 10.09 VDD471 pKa = 5.24 AGAGNDD477 pKa = 3.64 TVFTGGGSDD486 pKa = 3.71 TILGGEE492 pKa = 4.92 GDD494 pKa = 4.34 DD495 pKa = 4.3 QLHH498 pKa = 6.64 AEE500 pKa = 4.52 AGDD503 pKa = 3.63 DD504 pKa = 3.99 LVYY507 pKa = 11.03 GGAGADD513 pKa = 3.63 TLWGNEE519 pKa = 4.11 GADD522 pKa = 3.97 TLWGGDD528 pKa = 3.5 GADD531 pKa = 4.53 SILGGIGDD539 pKa = 4.05 DD540 pKa = 5.08 LIYY543 pKa = 11.25 GEE545 pKa = 5.27 AGNDD549 pKa = 3.82 TITGGDD555 pKa = 3.62 GADD558 pKa = 4.16 SISGGADD565 pKa = 2.9 NDD567 pKa = 4.27 VITGDD572 pKa = 4.74 LGDD575 pKa = 4.26 DD576 pKa = 3.71 TLAGGTGADD585 pKa = 3.73 TLTGGTGADD594 pKa = 3.59 TYY596 pKa = 11.79 VMSDD600 pKa = 2.87 GDD602 pKa = 4.01 GADD605 pKa = 3.24 TVTDD609 pKa = 3.91 FDD611 pKa = 3.72 MTMVSGLTTDD621 pKa = 3.29 QFDD624 pKa = 3.74 VSGLKK629 pKa = 10.18 DD630 pKa = 3.42 AEE632 pKa = 4.28 GNPVNVWDD640 pKa = 3.92 VTVDD644 pKa = 3.33 SDD646 pKa = 3.85 EE647 pKa = 4.14 QGNAVLFFPDD657 pKa = 3.75 GTSVTLIGVSKK668 pKa = 9.65 TSVNTAPMLHH678 pKa = 6.68 AMGIPCLVAGSPVATPRR695 pKa = 11.84 GPVAVEE701 pKa = 3.42 QLVPGDD707 pKa = 3.93 LVITASGAAEE717 pKa = 3.74 PVLWTGARR725 pKa = 11.84 AVTAAEE731 pKa = 4.03 MAADD735 pKa = 3.88 PRR737 pKa = 11.84 LLPVEE742 pKa = 4.51 IGAGRR747 pKa = 11.84 LGNAAPVRR755 pKa = 11.84 LSALHH760 pKa = 6.12 AVHH763 pKa = 6.47 VPARR767 pKa = 11.84 GGALARR773 pKa = 11.84 AGHH776 pKa = 5.87 MAATGWGGARR786 pKa = 11.84 ILHH789 pKa = 5.67 GLARR793 pKa = 11.84 QEE795 pKa = 3.63 AGLRR799 pKa = 11.84 YY800 pKa = 9.25 HH801 pKa = 6.89 HH802 pKa = 7.04 LLLPRR807 pKa = 11.84 HH808 pKa = 5.83 ALISVAGLWVEE819 pKa = 4.26 SFWPGRR825 pKa = 11.84 QGLAGLDD832 pKa = 3.5 PASRR836 pKa = 11.84 TRR838 pKa = 11.84 LIRR841 pKa = 11.84 ARR843 pKa = 11.84 PQLAQVLWGGASVAACYY860 pKa = 9.04 SQPAAPFLRR869 pKa = 11.84 RR870 pKa = 11.84 RR871 pKa = 11.84 EE872 pKa = 3.9 IDD874 pKa = 3.38 RR875 pKa = 11.84 AACSNWSLLARR886 pKa = 11.84 EE887 pKa = 4.9 KK888 pKa = 10.36 PHH890 pKa = 7.43 SDD892 pKa = 2.79 GFQASSGQASTLL904 pKa = 3.57
Molecular weight: 91.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.528
IPC_protein 3.605
Toseland 3.35
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.592
Rodwell 3.414
Grimsley 3.249
Solomon 3.617
Lehninger 3.567
Nozaki 3.719
DTASelect 4.05
Thurlkill 3.414
EMBOSS 3.592
Sillero 3.732
Patrickios 0.769
IPC_peptide 3.605
IPC2_peptide 3.694
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|A0A285RL35|A0A285RL35_9RHOB SSU ribosomal protein S13P OS=Rhodobacter maris OX=446682 GN=SAMN05877831_101642 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.43 LVRR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.38 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.43 GGRR28 pKa = 11.84 KK29 pKa = 8.99 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.53 GRR39 pKa = 11.84 KK40 pKa = 8.73 VLSAA44 pKa = 4.05
Molecular weight: 5.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3687
0
3687
1149499
25
2405
311.8
33.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.953 ± 0.073
0.906 ± 0.013
5.335 ± 0.043
6.137 ± 0.041
3.602 ± 0.025
8.843 ± 0.044
1.98 ± 0.019
4.807 ± 0.027
3.031 ± 0.033
10.565 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.657 ± 0.02
2.192 ± 0.022
5.431 ± 0.038
2.921 ± 0.024
7.078 ± 0.047
4.801 ± 0.027
5.28 ± 0.029
7.089 ± 0.033
1.361 ± 0.016
2.031 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here