Acetobacteraceae bacterium
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1743 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P7W745|A0A4P7W745_9PROT Lytic murein transglycosylase OS=Acetobacteraceae bacterium OX=1909293 GN=FAI41_00350 PE=4 SV=1
MM1 pKa = 6.73 NTQYY5 pKa = 11.3 DD6 pKa = 3.95 DD7 pKa = 4.75 SEE9 pKa = 4.37 EE10 pKa = 4.28 SPNPSSSHH18 pKa = 5.35 YY19 pKa = 10.06 VDD21 pKa = 4.01 EE22 pKa = 4.48 EE23 pKa = 4.17 AVIEE27 pKa = 4.89 AISEE31 pKa = 4.39 VYY33 pKa = 10.58 DD34 pKa = 3.45 PEE36 pKa = 5.22 IPVNVYY42 pKa = 10.83 DD43 pKa = 5.18 LGLIYY48 pKa = 10.47 AIDD51 pKa = 3.63 LHH53 pKa = 7.92 DD54 pKa = 5.15 DD55 pKa = 3.24 GRR57 pKa = 11.84 VIVEE61 pKa = 4.2 MSLTAPNCPSAQEE74 pKa = 4.13 LPEE77 pKa = 4.27 MIRR80 pKa = 11.84 DD81 pKa = 3.67 SVATVKK87 pKa = 10.71 NVTNVEE93 pKa = 4.33 VKK95 pKa = 10.27 VVWDD99 pKa = 4.54 PPWEE103 pKa = 4.0 MSRR106 pKa = 11.84 MSDD109 pKa = 3.8 AARR112 pKa = 11.84 LALNLFF118 pKa = 4.25
Molecular weight: 13.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.973
IPC2_protein 3.91
IPC_protein 3.859
Toseland 3.668
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.821
Lehninger 3.783
Nozaki 3.961
DTASelect 4.139
Thurlkill 3.719
EMBOSS 3.757
Sillero 3.973
Patrickios 2.842
IPC_peptide 3.821
IPC2_peptide 3.961
IPC2.peptide.svr19 3.89
Protein with the highest isoelectric point:
>tr|A0A4P7WB64|A0A4P7WB64_9PROT Probable multidrug resistance protein NorM OS=Acetobacteraceae bacterium OX=1909293 GN=FAI41_08560 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.64 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 SATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.1 GRR39 pKa = 11.84 KK40 pKa = 8.83 KK41 pKa = 10.63 LSAA44 pKa = 3.95
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1743
0
1743
564209
41
2264
323.7
35.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.912 ± 0.061
1.109 ± 0.021
4.728 ± 0.039
6.759 ± 0.074
4.689 ± 0.05
7.305 ± 0.082
2.262 ± 0.025
6.332 ± 0.043
6.47 ± 0.058
10.671 ± 0.08
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.216 ± 0.023
3.981 ± 0.048
4.906 ± 0.038
3.937 ± 0.035
4.849 ± 0.053
6.654 ± 0.045
4.832 ± 0.047
5.432 ± 0.054
1.349 ± 0.024
2.609 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here