Actinomyces virus Av1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Dybvigvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q45NF1|Q45NF1_9CAUD Holin OS=Actinomyces virus Av1 OX=338473 GN=holB PE=4 SV=1
MM1 pKa = 7.46ATRR4 pKa = 11.84ADD6 pKa = 3.6IIAAAKK12 pKa = 10.67AEE14 pKa = 4.0IGYY17 pKa = 9.2SRR19 pKa = 11.84WADD22 pKa = 3.43DD23 pKa = 3.91EE24 pKa = 5.9AGTKK28 pKa = 9.02YY29 pKa = 10.36GRR31 pKa = 11.84WYY33 pKa = 10.58AQVTGSPSFGASGVPYY49 pKa = 10.65CDD51 pKa = 3.84MFVSWVLSKK60 pKa = 11.32VGINWVSAYY69 pKa = 10.23VPSRR73 pKa = 11.84EE74 pKa = 3.96AQARR78 pKa = 11.84ARR80 pKa = 11.84GVLIDD85 pKa = 3.49KK86 pKa = 10.03WDD88 pKa = 3.54VRR90 pKa = 11.84LGDD93 pKa = 4.66LMTFDD98 pKa = 5.14FDD100 pKa = 4.56GEE102 pKa = 4.63GIAQHH107 pKa = 6.34IGIVDD112 pKa = 3.75QPPNSAGVFYY122 pKa = 11.0SVDD125 pKa = 3.51GNTTWGTGGPQDD137 pKa = 3.4NGGVVARR144 pKa = 11.84RR145 pKa = 11.84EE146 pKa = 3.93RR147 pKa = 11.84HH148 pKa = 5.49IDD150 pKa = 3.1QARR153 pKa = 11.84YY154 pKa = 8.75GIRR157 pKa = 11.84VIDD160 pKa = 4.47DD161 pKa = 3.32NSAISSGGNIVEE173 pKa = 4.19IQRR176 pKa = 11.84ILGAVQDD183 pKa = 4.12NVLGVDD189 pKa = 3.46TEE191 pKa = 4.16KK192 pKa = 10.93RR193 pKa = 11.84MCAVIKK199 pKa = 10.38ASNWGGRR206 pKa = 11.84EE207 pKa = 3.96FPWGVAYY214 pKa = 7.79TQQVVGTVPDD224 pKa = 4.62GVWGDD229 pKa = 3.68ASEE232 pKa = 4.38AAHH235 pKa = 6.51DD236 pKa = 3.9RR237 pKa = 11.84VIEE240 pKa = 4.13SLQGALGVTIDD251 pKa = 4.33GVWGPEE257 pKa = 3.34TWAAWEE263 pKa = 3.98RR264 pKa = 11.84LARR267 pKa = 11.84TAEE270 pKa = 4.06RR271 pKa = 11.84PP272 pKa = 3.44

Molecular weight: 29.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6XAE3|A6XAE3_9CAUD Uncharacterized protein OS=Actinomyces virus Av1 OX=338473 PE=4 SV=1
MM1 pKa = 7.48SIVIPRR7 pKa = 11.84KK8 pKa = 8.36WLVPSGGITVYY19 pKa = 10.66GRR21 pKa = 11.84SAVRR25 pKa = 11.84ARR27 pKa = 11.84RR28 pKa = 11.84SQAAQVSGPHH38 pKa = 5.68TPSMVTPRR46 pKa = 11.84APCRR50 pKa = 11.84DD51 pKa = 3.22SMTRR55 pKa = 11.84SCAASLASPQTPSGTVPTTCCVV77 pKa = 3.01

Molecular weight: 8.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22

0

22

5461

40

731

248.2

27.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.167 ± 0.58

0.714 ± 0.139

6.592 ± 0.48

5.127 ± 0.528

3.552 ± 0.49

7.947 ± 0.632

1.63 ± 0.226

6.208 ± 0.394

5.878 ± 0.786

6.171 ± 0.321

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.857 ± 0.236

5.53 ± 0.475

3.992 ± 0.331

3.296 ± 0.269

5.768 ± 0.356

7.435 ± 0.379

6.391 ± 0.489

6.611 ± 0.336

1.904 ± 0.161

4.23 ± 0.394
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski