Thermus virus IN93
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q859S7|Q859S7_9VIRU Uncharacterized protein OS=Thermus virus IN93 OX=1714273 PE=4 SV=1
MM1 pKa = 7.36 SFSTSQYY8 pKa = 10.73 LDD10 pKa = 3.84 DD11 pKa = 4.57 GLYY14 pKa = 10.4 SGSSGSSDD22 pKa = 3.15 FGLSNSYY29 pKa = 8.23 TLPLTGSSSSGSSDD43 pKa = 3.17 FGLSNSYY50 pKa = 10.28 PFLWAPTGDD59 pKa = 3.63 TPLWAQILGGLGQIGSLIDD78 pKa = 3.89 PYY80 pKa = 10.94 ILTEE84 pKa = 3.92 QEE86 pKa = 3.71 RR87 pKa = 11.84 LQYY90 pKa = 10.54 QLQLAQAQAEE100 pKa = 4.21 AAKK103 pKa = 10.19 AQAASVQAQPAVPVWVWVLVGLGVVLLLVFALKK136 pKa = 10.25 EE137 pKa = 3.89
Molecular weight: 14.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.828
IPC2_protein 3.846
IPC_protein 3.719
Toseland 3.528
ProMoST 3.846
Dawson 3.719
Bjellqvist 3.999
Wikipedia 3.694
Rodwell 3.554
Grimsley 3.452
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.846
Patrickios 0.299
IPC_peptide 3.681
IPC2_peptide 3.808
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|Q859T1|Q859T1_9VIRU Uncharacterized protein OS=Thermus virus IN93 OX=1714273 PE=4 SV=1
MM1 pKa = 7.85 GGRR4 pKa = 11.84 QTFRR8 pKa = 11.84 ILIVGKK14 pKa = 10.08 SGSGKK19 pKa = 8.62 STLARR24 pKa = 11.84 QIVRR28 pKa = 11.84 AMEE31 pKa = 3.84 GRR33 pKa = 11.84 FRR35 pKa = 11.84 RR36 pKa = 11.84 LIIVNRR42 pKa = 11.84 KK43 pKa = 6.74 TEE45 pKa = 4.1 FGEE48 pKa = 4.0 LAEE51 pKa = 3.93 ARR53 pKa = 11.84 YY54 pKa = 9.33 RR55 pKa = 11.84 VGEE58 pKa = 5.07 DD59 pKa = 3.33 GDD61 pKa = 3.63 PWPVLKK67 pKa = 10.4 RR68 pKa = 11.84 HH69 pKa = 5.74 RR70 pKa = 11.84 RR71 pKa = 11.84 VHH73 pKa = 5.07 FHH75 pKa = 4.41 VTGYY79 pKa = 10.74 DD80 pKa = 3.33 PRR82 pKa = 11.84 PFLDD86 pKa = 3.99 ALGQAIMRR94 pKa = 11.84 LRR96 pKa = 11.84 DD97 pKa = 3.53 VLLLLDD103 pKa = 4.27 EE104 pKa = 4.58 AHH106 pKa = 6.91 HH107 pKa = 6.15 FFPRR111 pKa = 11.84 GQVPKK116 pKa = 10.89 GLFEE120 pKa = 4.2 VLTGGRR126 pKa = 11.84 EE127 pKa = 4.02 FGHH130 pKa = 5.84 SAIFVTQMLQAATGGIDD147 pKa = 3.12 PGVRR151 pKa = 11.84 RR152 pKa = 11.84 QASHH156 pKa = 6.68 LVAFRR161 pKa = 11.84 LTEE164 pKa = 3.95 PRR166 pKa = 11.84 EE167 pKa = 4.11 VQALADD173 pKa = 3.92 MFPEE177 pKa = 4.23 LGEE180 pKa = 3.87 RR181 pKa = 11.84 VRR183 pKa = 11.84 ALKK186 pKa = 10.6 RR187 pKa = 11.84 PDD189 pKa = 4.45 DD190 pKa = 4.44 GLPPEE195 pKa = 4.85 YY196 pKa = 10.32 GVKK199 pKa = 10.71 DD200 pKa = 3.86 LDD202 pKa = 3.62 RR203 pKa = 11.84 DD204 pKa = 3.55 RR205 pKa = 11.84 AGLVLRR211 pKa = 11.84 DD212 pKa = 3.33 PRR214 pKa = 11.84 APQRR218 pKa = 11.84 RR219 pKa = 11.84 VFMPLDD225 pKa = 3.52 GG226 pKa = 4.64
Molecular weight: 25.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.335
IPC2_protein 9.472
IPC_protein 10.496
Toseland 10.73
ProMoST 10.599
Dawson 10.804
Bjellqvist 10.57
Wikipedia 11.067
Rodwell 10.818
Grimsley 10.847
Solomon 11.023
Lehninger 10.965
Nozaki 10.716
DTASelect 10.57
Thurlkill 10.73
EMBOSS 11.169
Sillero 10.745
Patrickios 10.54
IPC_peptide 11.023
IPC2_peptide 9.487
IPC2.peptide.svr19 8.858
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
39
0
39
5911
37
412
151.6
16.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.352 ± 0.579
0.829 ± 0.162
4.314 ± 0.209
6.344 ± 0.53
2.944 ± 0.293
9.406 ± 0.407
1.59 ± 0.263
3.367 ± 0.235
3.13 ± 0.342
10.387 ± 0.47
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.962 ± 0.173
2.284 ± 0.339
7.308 ± 0.404
4.179 ± 0.325
8.323 ± 0.548
4.517 ± 0.468
4.483 ± 0.48
7.884 ± 0.367
2.284 ± 0.23
3.113 ± 0.243
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here