Firmicutes bacterium CAG:102

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2021 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5A8U1|R5A8U1_9FIRM Ion-translocating oxidoreductase complex subunit A OS=Firmicutes bacterium CAG:102 OX=1262998 GN=rnfA PE=3 SV=1
MM1 pKa = 7.91EE2 pKa = 4.64YY3 pKa = 10.46FEE5 pKa = 6.04KK6 pKa = 10.61KK7 pKa = 7.88ITYY10 pKa = 9.89LRR12 pKa = 11.84GLCDD16 pKa = 3.65GSDD19 pKa = 3.98FPAEE23 pKa = 4.21SKK25 pKa = 10.15EE26 pKa = 3.7GKK28 pKa = 9.43IFHH31 pKa = 7.2GILDD35 pKa = 4.14VLDD38 pKa = 5.44DD39 pKa = 3.91MAFMLEE45 pKa = 4.07TFMDD49 pKa = 4.54DD50 pKa = 3.06EE51 pKa = 4.82DD52 pKa = 5.76LVMEE56 pKa = 5.69DD57 pKa = 4.18EE58 pKa = 5.75DD59 pKa = 5.79LDD61 pKa = 4.06EE62 pKa = 4.39TDD64 pKa = 4.12EE65 pKa = 5.09PIFFYY70 pKa = 11.02SFNCPSCGQEE80 pKa = 4.24LDD82 pKa = 3.66VDD84 pKa = 4.1EE85 pKa = 4.96QTMEE89 pKa = 4.01MQTEE93 pKa = 4.82IPCPGCGATVPIGSADD109 pKa = 3.53MDD111 pKa = 3.96DD112 pKa = 4.57LKK114 pKa = 11.38FF115 pKa = 3.92

Molecular weight: 13.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5ABW2|R5ABW2_9FIRM ATP-dependent zinc metalloprotease FtsH OS=Firmicutes bacterium CAG:102 OX=1262998 GN=ftsH PE=3 SV=1
MM1 pKa = 7.53LLSDD5 pKa = 4.16ISRR8 pKa = 11.84GFYY11 pKa = 10.48NLRR14 pKa = 11.84GCLNGFCIGDD24 pKa = 4.25FYY26 pKa = 11.6HH27 pKa = 6.74FAFAAWQRR35 pKa = 11.84KK36 pKa = 6.86HH37 pKa = 6.84RR38 pKa = 11.84NDD40 pKa = 4.83FISCRR45 pKa = 11.84QIIGKK50 pKa = 9.94CNFTAVCGRR59 pKa = 11.84RR60 pKa = 11.84YY61 pKa = 8.98RR62 pKa = 11.84TGIQQSISYY71 pKa = 9.26ICTVKK76 pKa = 10.0CTTIAVGIQLQIACKK91 pKa = 9.76RR92 pKa = 11.84IVEE95 pKa = 3.71IRR97 pKa = 11.84LYY99 pKa = 10.34IKK101 pKa = 10.37ILIRR105 pKa = 11.84RR106 pKa = 11.84KK107 pKa = 10.33NFGGGNFNRR116 pKa = 11.84RR117 pKa = 11.84LFYY120 pKa = 10.86HH121 pKa = 7.3LATNQNINLIITVMGSASVNIIFVKK146 pKa = 9.63IYY148 pKa = 10.0QYY150 pKa = 11.0IFFRR154 pKa = 11.84RR155 pKa = 11.84KK156 pKa = 8.04ISLYY160 pKa = 10.05IKK162 pKa = 10.44GIAAASRR169 pKa = 11.84KK170 pKa = 9.82ALTLEE175 pKa = 3.93GCRR178 pKa = 11.84IIRR181 pKa = 11.84MCIVSVCKK189 pKa = 9.78PYY191 pKa = 11.16KK192 pKa = 10.45HH193 pKa = 6.19IAALIAVISVYY204 pKa = 10.52GNRR207 pKa = 11.84NAFRR211 pKa = 11.84ICCEE215 pKa = 4.1CFGGNADD222 pKa = 3.39AVPFRR227 pKa = 11.84FRR229 pKa = 11.84FYY231 pKa = 11.01RR232 pKa = 11.84KK233 pKa = 8.94GCAGVIMRR241 pKa = 11.84FIFIINIIDD250 pKa = 3.24IRR252 pKa = 11.84NNHH255 pKa = 5.58IPCRR259 pKa = 3.91

Molecular weight: 29.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2021

0

2021

601679

30

2943

297.7

33.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.956 ± 0.062

1.445 ± 0.024

5.308 ± 0.049

7.941 ± 0.068

4.085 ± 0.037

7.438 ± 0.051

1.708 ± 0.023

6.727 ± 0.051

6.806 ± 0.054

9.294 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.108 ± 0.033

3.854 ± 0.043

3.341 ± 0.028

3.197 ± 0.033

4.697 ± 0.044

5.042 ± 0.034

5.642 ± 0.058

6.896 ± 0.052

0.866 ± 0.02

3.644 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski