Penicillium stoloniferum virus S
Average proteome isoelectric point is 7.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6YDQ7|Q6YDQ7_9VIRU RNA-dependent RNA polymerase OS=Penicillium stoloniferum virus S OX=216371 PE=4 SV=2
MM1 pKa = 7.51 SSIAPTDD8 pKa = 3.89 SVSSSGKK15 pKa = 8.95 RR16 pKa = 11.84 SKK18 pKa = 10.08 PGKK21 pKa = 9.7 RR22 pKa = 11.84 EE23 pKa = 3.7 RR24 pKa = 11.84 QQARR28 pKa = 11.84 SAVGSAGGKK37 pKa = 8.09 PASASKK43 pKa = 10.42 AAAFAQGGSSDD54 pKa = 4.2 PVPMPGKK61 pKa = 9.55 YY62 pKa = 9.27 PVVFSTGAGEE72 pKa = 3.96 PTRR75 pKa = 11.84 DD76 pKa = 3.34 QEE78 pKa = 4.59 FALPVHH84 pKa = 6.38 KK85 pKa = 10.51 AFPLFGSVSDD95 pKa = 3.79 KK96 pKa = 10.51 YY97 pKa = 10.86 RR98 pKa = 11.84 RR99 pKa = 11.84 NPRR102 pKa = 11.84 YY103 pKa = 10.13 AEE105 pKa = 4.01 FRR107 pKa = 11.84 AHH109 pKa = 7.24 SEE111 pKa = 3.85 FTDD114 pKa = 4.5 GVFGTHH120 pKa = 6.65 LAVSSLLRR128 pKa = 11.84 LAQQLVHH135 pKa = 6.26 AHH137 pKa = 5.26 VNMGLPLGDD146 pKa = 4.44 FAPLASSDD154 pKa = 3.46 VRR156 pKa = 11.84 IPSALASVVNQFGEE170 pKa = 4.48 FSSPSIGTRR179 pKa = 11.84 FLLRR183 pKa = 11.84 DD184 pKa = 3.45 YY185 pKa = 9.6 EE186 pKa = 4.44 HH187 pKa = 6.79 AVSRR191 pKa = 11.84 VVFLADD197 pKa = 3.8 QLWTNGNSHH206 pKa = 7.21 HH207 pKa = 6.5 IFARR211 pKa = 11.84 SWLPMSNNDD220 pKa = 3.6 GNFKK224 pKa = 10.39 TIVASRR230 pKa = 11.84 LLEE233 pKa = 4.65 FISAGDD239 pKa = 3.68 LSILPTVLEE248 pKa = 4.33 DD249 pKa = 3.41 AVLSGEE255 pKa = 4.23 VPEE258 pKa = 4.98 AWEE261 pKa = 3.96 QVKK264 pKa = 10.74 DD265 pKa = 4.08 LLGDD269 pKa = 3.7 APGVGQVDD277 pKa = 3.11 RR278 pKa = 11.84 RR279 pKa = 11.84 DD280 pKa = 3.44 RR281 pKa = 11.84 FDD283 pKa = 5.13 FLFKK287 pKa = 10.7 SYY289 pKa = 11.52 ADD291 pKa = 3.29 VGQFTTAFTTQAASDD306 pKa = 3.84 VLTEE310 pKa = 6.13 LGLPWNSPSAGHH322 pKa = 7.03 LNWQYY327 pKa = 9.3 STKK330 pKa = 10.3 QRR332 pKa = 11.84 FTFLADD338 pKa = 3.24 TWAKK342 pKa = 10.61 LSAAYY347 pKa = 10.16 SQFFEE352 pKa = 4.74 LSSGLATRR360 pKa = 11.84 QSATGSHH367 pKa = 5.79 AQMVDD372 pKa = 3.26 LTSVEE377 pKa = 4.19 GVTVLKK383 pKa = 10.71 AALALSAPEE392 pKa = 4.39 FSLAACFPPSCIFVGGITRR411 pKa = 11.84 RR412 pKa = 11.84 VVVTTSLSVSQRR424 pKa = 11.84 ATEE427 pKa = 4.57 FCQMDD432 pKa = 3.2 WRR434 pKa = 5.42
Molecular weight: 46.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.662
IPC2_protein 6.62
IPC_protein 6.883
Toseland 7.015
ProMoST 7.424
Dawson 7.351
Bjellqvist 7.161
Wikipedia 7.337
Rodwell 7.337
Grimsley 7.132
Solomon 7.424
Lehninger 7.439
Nozaki 7.512
DTASelect 7.614
Thurlkill 7.658
EMBOSS 7.717
Sillero 7.717
Patrickios 4.673
IPC_peptide 7.424
IPC2_peptide 7.19
IPC2.peptide.svr19 7.069
Protein with the highest isoelectric point:
>tr|Q6YDQ7|Q6YDQ7_9VIRU RNA-dependent RNA polymerase OS=Penicillium stoloniferum virus S OX=216371 PE=4 SV=2
MM1 pKa = 7.81 EE2 pKa = 6.64 DD3 pKa = 3.67 SPFDD7 pKa = 3.66 PTLLDD12 pKa = 3.48 VAVEE16 pKa = 4.08 EE17 pKa = 4.37 SHH19 pKa = 7.24 LVDD22 pKa = 5.76 DD23 pKa = 5.71 SPLTPSNRR31 pKa = 11.84 TRR33 pKa = 11.84 GASYY37 pKa = 10.61 GIISEE42 pKa = 4.51 KK43 pKa = 10.35 FSSPGLRR50 pKa = 11.84 EE51 pKa = 3.35 IARR54 pKa = 11.84 YY55 pKa = 9.42 GGYY58 pKa = 9.58 SVYY61 pKa = 10.57 SGQSNTDD68 pKa = 2.27 AWIRR72 pKa = 11.84 TTLKK76 pKa = 10.12 EE77 pKa = 3.99 FDD79 pKa = 3.29 RR80 pKa = 11.84 SVYY83 pKa = 10.91 DD84 pKa = 4.61 DD85 pKa = 3.2 IYY87 pKa = 10.86 GYY89 pKa = 7.49 TRR91 pKa = 11.84 RR92 pKa = 11.84 PTGTLGMYY100 pKa = 10.6 GSLLKK105 pKa = 10.71 FSEE108 pKa = 4.48 GKK110 pKa = 10.39 NSFASLNRR118 pKa = 11.84 VQRR121 pKa = 11.84 KK122 pKa = 8.99 SMINAISKK130 pKa = 9.92 AKK132 pKa = 10.2 KK133 pKa = 9.99 AFKK136 pKa = 10.37 LPYY139 pKa = 9.5 QRR141 pKa = 11.84 EE142 pKa = 3.99 PLDD145 pKa = 3.08 WHH147 pKa = 5.46 EE148 pKa = 4.44 VGRR151 pKa = 11.84 HH152 pKa = 4.2 FRR154 pKa = 11.84 RR155 pKa = 11.84 DD156 pKa = 3.05 TSAGVSFMGQKK167 pKa = 10.24 KK168 pKa = 9.61 GDD170 pKa = 3.65 VMEE173 pKa = 5.22 EE174 pKa = 4.04 IYY176 pKa = 10.96 HH177 pKa = 5.05 EE178 pKa = 4.4 ARR180 pKa = 11.84 WLGHH184 pKa = 5.84 RR185 pKa = 11.84 MKK187 pKa = 10.97 QNGRR191 pKa = 11.84 AKK193 pKa = 10.37 FDD195 pKa = 3.77 PSKK198 pKa = 10.71 MRR200 pKa = 11.84 FPPCLAGQRR209 pKa = 11.84 GGMSEE214 pKa = 4.39 ASDD217 pKa = 3.44 PKK219 pKa = 10.64 TRR221 pKa = 11.84 LVWIYY226 pKa = 9.11 PAEE229 pKa = 3.94 MLAIEE234 pKa = 5.05 GFYY237 pKa = 10.75 APEE240 pKa = 4.22 MYY242 pKa = 9.71 HH243 pKa = 8.87 AYY245 pKa = 9.47 MDD247 pKa = 4.96 DD248 pKa = 3.94 PLSPMLNGKK257 pKa = 9.39 SSQRR261 pKa = 11.84 LYY263 pKa = 10.75 TEE265 pKa = 4.47 WTCGLRR271 pKa = 11.84 DD272 pKa = 3.36 GEE274 pKa = 4.33 MLYY277 pKa = 11.21 GLDD280 pKa = 4.39 FSGFDD285 pKa = 3.33 TKK287 pKa = 11.07 VPAWLIRR294 pKa = 11.84 VAFDD298 pKa = 3.86 ILRR301 pKa = 11.84 QNIRR305 pKa = 11.84 WDD307 pKa = 3.36 SFRR310 pKa = 11.84 GEE312 pKa = 4.2 KK313 pKa = 9.8 VSKK316 pKa = 10.5 RR317 pKa = 11.84 DD318 pKa = 3.4 AQKK321 pKa = 10.19 WRR323 pKa = 11.84 NVWDD327 pKa = 3.58 GMVWYY332 pKa = 8.64 FINTPILMPDD342 pKa = 2.41 GRR344 pKa = 11.84 MFRR347 pKa = 11.84 KK348 pKa = 9.98 YY349 pKa = 10.14 RR350 pKa = 11.84 GVPSGSWFTQMIDD363 pKa = 3.34 SVVNYY368 pKa = 10.2 ILVDD372 pKa = 3.46 YY373 pKa = 10.57 LAACQQCEE381 pKa = 3.73 IRR383 pKa = 11.84 ALRR386 pKa = 11.84 VLGDD390 pKa = 3.57 DD391 pKa = 3.83 SAFRR395 pKa = 11.84 SCDD398 pKa = 3.48 PFSLDD403 pKa = 4.39 LASHH407 pKa = 6.92 DD408 pKa = 4.04 AEE410 pKa = 4.35 CVNMILHH417 pKa = 6.69 PEE419 pKa = 3.98 KK420 pKa = 10.75 CEE422 pKa = 3.91 KK423 pKa = 10.35 TKK425 pKa = 11.24 DD426 pKa = 3.27 PTAFKK431 pKa = 11.03 LLGTTYY437 pKa = 10.92 RR438 pKa = 11.84 NGRR441 pKa = 11.84 PHH443 pKa = 7.54 RR444 pKa = 11.84 EE445 pKa = 3.68 TNEE448 pKa = 3.71 WFKK451 pKa = 11.1 LALYY455 pKa = 9.01 PEE457 pKa = 4.62 SVVPSLQVSFTRR469 pKa = 11.84 LIGLWIGGAMFDD481 pKa = 4.74 SRR483 pKa = 11.84 FCQFMEE489 pKa = 4.47 YY490 pKa = 10.51 YY491 pKa = 8.36 QTCFPCPQEE500 pKa = 3.82 GWFSKK505 pKa = 9.82 DD506 pKa = 3.0 QRR508 pKa = 11.84 RR509 pKa = 11.84 WLQVVYY515 pKa = 10.42 GGKK518 pKa = 10.08 APRR521 pKa = 11.84 GWTTKK526 pKa = 10.44 RR527 pKa = 11.84 SLFWRR532 pKa = 11.84 SIFYY536 pKa = 10.87 VYY538 pKa = 10.99 GG539 pKa = 3.59
Molecular weight: 62.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.171
IPC2_protein 8.244
IPC_protein 8.112
Toseland 8.229
ProMoST 8.609
Dawson 8.887
Bjellqvist 8.99
Wikipedia 8.96
Rodwell 8.946
Grimsley 8.39
Solomon 8.96
Lehninger 8.931
Nozaki 9.063
DTASelect 8.814
Thurlkill 8.858
EMBOSS 9.019
Sillero 9.107
Patrickios 4.355
IPC_peptide 8.946
IPC2_peptide 7.849
IPC2.peptide.svr19 7.864
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
973
434
539
486.5
54.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.119 ± 2.071
1.336 ± 0.421
5.755 ± 0.448
4.625 ± 0.462
6.064 ± 0.404
7.605 ± 0.152
1.953 ± 0.229
3.186 ± 0.726
4.419 ± 0.779
8.222 ± 0.499
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.672 ± 0.842
2.467 ± 0.106
5.447 ± 0.054
3.803 ± 0.376
7.091 ± 0.869
9.764 ± 1.448
5.139 ± 0.406
6.372 ± 1.105
2.467 ± 0.557
3.494 ± 1.229
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here