Flammeovirgaceae bacterium 311

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Flammeovirgaceae; unclassified Flammeovirgaceae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3LIH2|A0A0D3LIH2_9BACT 4a-hydroxytetrahydrobiopterin dehydratase OS=Flammeovirgaceae bacterium 311 OX=1257021 GN=D770_17335 PE=3 SV=1
MM1 pKa = 7.52KK2 pKa = 10.49KK3 pKa = 8.67STLKK7 pKa = 10.65KK8 pKa = 9.01YY9 pKa = 9.59FLWSTLLLYY18 pKa = 9.26FMWSSNLVKK27 pKa = 10.11ATPAEE32 pKa = 4.14AAPALIFTSPGYY44 pKa = 10.27SEE46 pKa = 4.74NNKK49 pKa = 10.04NSLAAEE55 pKa = 4.41DD56 pKa = 4.17ADD58 pKa = 3.9GDD60 pKa = 4.38GVEE63 pKa = 4.74DD64 pKa = 4.31TVDD67 pKa = 3.52NCPSNYY73 pKa = 9.83NPGQEE78 pKa = 5.21DD79 pKa = 3.66MDD81 pKa = 5.62GDD83 pKa = 4.73GIGDD87 pKa = 3.84ACDD90 pKa = 4.66DD91 pKa = 5.0DD92 pKa = 4.19MDD94 pKa = 6.38GDD96 pKa = 4.16GALNEE101 pKa = 4.7LDD103 pKa = 5.05CNPNDD108 pKa = 3.86PADD111 pKa = 3.79SGEE114 pKa = 4.0YY115 pKa = 9.55WFADD119 pKa = 3.3EE120 pKa = 5.93DD121 pKa = 4.54GDD123 pKa = 4.89GFGSQNEE130 pKa = 4.09ILACEE135 pKa = 4.0QPEE138 pKa = 4.9GYY140 pKa = 10.05VDD142 pKa = 5.69NYY144 pKa = 11.47DD145 pKa = 4.07DD146 pKa = 6.2CNDD149 pKa = 3.53NNPNINPEE157 pKa = 4.2AEE159 pKa = 4.36DD160 pKa = 4.37DD161 pKa = 4.29PYY163 pKa = 12.11DD164 pKa = 5.12NIDD167 pKa = 4.06SNCDD171 pKa = 3.38GIDD174 pKa = 4.26PIGSDD179 pKa = 3.68ADD181 pKa = 3.96LDD183 pKa = 4.36GVDD186 pKa = 5.62DD187 pKa = 6.37LYY189 pKa = 11.68DD190 pKa = 4.06NCPDD194 pKa = 3.53TYY196 pKa = 11.27NPKK199 pKa = 10.0QEE201 pKa = 4.32DD202 pKa = 3.99TNEE205 pKa = 4.07DD206 pKa = 3.98GIGDD210 pKa = 3.81VCDD213 pKa = 5.11DD214 pKa = 4.88DD215 pKa = 4.72VAEE218 pKa = 4.35NQAPVITILDD228 pKa = 3.98YY229 pKa = 11.34DD230 pKa = 3.86PEE232 pKa = 4.98KK233 pKa = 10.82YY234 pKa = 10.44YY235 pKa = 11.0YY236 pKa = 10.67AAGQEE241 pKa = 4.42HH242 pKa = 6.76VFTIQVTDD250 pKa = 3.88PDD252 pKa = 4.08RR253 pKa = 11.84LEE255 pKa = 5.1LLLLDD260 pKa = 4.44IEE262 pKa = 4.67YY263 pKa = 10.97NKK265 pKa = 10.43NAYY268 pKa = 9.21QPGEE272 pKa = 4.14LTFSPSLPVRR282 pKa = 11.84GVGSLQIEE290 pKa = 4.7VRR292 pKa = 11.84WKK294 pKa = 10.26PNMEE298 pKa = 4.21RR299 pKa = 11.84PQHH302 pKa = 5.55LQLDD306 pKa = 4.9IIAMDD311 pKa = 4.14GDD313 pKa = 3.66RR314 pKa = 11.84EE315 pKa = 4.19RR316 pKa = 11.84TVKK319 pKa = 10.75YY320 pKa = 10.77LFGDD324 pKa = 4.25PYY326 pKa = 11.32LPTQISTPQEE336 pKa = 4.23PIVIGVDD343 pKa = 2.9QAFRR347 pKa = 11.84IDD349 pKa = 3.71IPFTADD355 pKa = 3.3FEE357 pKa = 4.57EE358 pKa = 4.66EE359 pKa = 3.96AYY361 pKa = 10.51AWFDD365 pKa = 3.62APEE368 pKa = 3.8WAADD372 pKa = 3.73DD373 pKa = 4.18NEE375 pKa = 4.41YY376 pKa = 9.35TLTLTNNEE384 pKa = 3.92FGMPAMTDD392 pKa = 2.76VGTYY396 pKa = 8.92QIPLGVSYY404 pKa = 9.66PWGTTRR410 pKa = 11.84STITVIVSAEE420 pKa = 3.95CTNQNWYY427 pKa = 9.11MDD429 pKa = 4.28EE430 pKa = 5.77DD431 pKa = 4.61GDD433 pKa = 5.35GYY435 pKa = 10.5GTQQTSAYY443 pKa = 9.84FPFRR447 pKa = 11.84QITCSSWPGYY457 pKa = 9.94VNNNDD462 pKa = 3.55DD463 pKa = 4.43CNDD466 pKa = 3.43NEE468 pKa = 4.84ASINPDD474 pKa = 2.97APEE477 pKa = 4.3IPGDD481 pKa = 4.87GIDD484 pKa = 3.93NNCNGQVDD492 pKa = 4.26DD493 pKa = 5.02GSTNTTAPYY502 pKa = 10.01FISSDD507 pKa = 2.91IGQEE511 pKa = 3.85ILVGGQFNFTIQVADD526 pKa = 3.62PTMGDD531 pKa = 3.5VVTLRR536 pKa = 11.84VDD538 pKa = 3.56SAAMINYY545 pKa = 8.46GSSGPPYY552 pKa = 10.3TLLPDD557 pKa = 3.98LAGATFSPDD566 pKa = 3.16RR567 pKa = 11.84SVTAADD573 pKa = 3.63TASLHH578 pKa = 5.83MNWTPTRR585 pKa = 11.84EE586 pKa = 4.22HH587 pKa = 6.9LGEE590 pKa = 4.04IYY592 pKa = 10.56FFFSATDD599 pKa = 3.17QHH601 pKa = 7.0GNSVWGFLPVVVDD614 pKa = 6.12LPFALQAVPPVVVGTQQPFEE634 pKa = 4.33VIIPISNSDD643 pKa = 2.89WHH645 pKa = 6.81QLDD648 pKa = 3.85EE649 pKa = 4.46VSLLEE654 pKa = 4.97GAPSWLQAALVSVDD668 pKa = 2.87GRR670 pKa = 11.84EE671 pKa = 3.91VPNAIRR677 pKa = 11.84VWGTPPARR685 pKa = 11.84SFGTYY690 pKa = 9.06KK691 pKa = 10.45AQVRR695 pKa = 11.84ARR697 pKa = 11.84TFWAKK702 pKa = 9.97WDD704 pKa = 3.8TLEE707 pKa = 4.4LQIRR711 pKa = 11.84VGNCTNRR718 pKa = 11.84AWYY721 pKa = 10.4ADD723 pKa = 3.46ADD725 pKa = 4.11GDD727 pKa = 5.17GFGADD732 pKa = 3.13NTMVTACWQPEE743 pKa = 4.68GYY745 pKa = 9.62VSKK748 pKa = 11.27GGDD751 pKa = 3.92CNDD754 pKa = 3.83ADD756 pKa = 3.44ATVYY760 pKa = 10.75PEE762 pKa = 4.35APEE765 pKa = 4.43LADD768 pKa = 5.23GKK770 pKa = 10.82DD771 pKa = 3.61NNCDD775 pKa = 3.19GMADD779 pKa = 3.59EE780 pKa = 5.48TDD782 pKa = 3.29NSCYY786 pKa = 8.64ATRR789 pKa = 11.84VISFTQGPRR798 pKa = 11.84ADD800 pKa = 3.42KK801 pKa = 10.81RR802 pKa = 11.84GVIDD806 pKa = 4.06PLRR809 pKa = 11.84SIPLRR814 pKa = 11.84ALGAPQEE821 pKa = 4.64DD822 pKa = 4.2KK823 pKa = 10.17THH825 pKa = 6.46SFVSLGFGGEE835 pKa = 4.16LVLEE839 pKa = 4.89LGANLYY845 pKa = 10.85DD846 pKa = 5.57DD847 pKa = 4.51GTTAPDD853 pKa = 3.48LMVVEE858 pKa = 5.0TTWGWANRR866 pKa = 11.84PCYY869 pKa = 9.76DD870 pKa = 3.27GKK872 pKa = 11.28GAGTLEE878 pKa = 3.99TMMMHH883 pKa = 6.34VSANGEE889 pKa = 3.88DD890 pKa = 3.91WVQVPGNFCRR900 pKa = 11.84NVKK903 pKa = 10.03VDD905 pKa = 3.4ISPVTGKK912 pKa = 10.67DD913 pKa = 2.88MLPYY917 pKa = 10.19VRR919 pKa = 11.84YY920 pKa = 9.95IKK922 pKa = 9.68ITDD925 pKa = 3.47TSDD928 pKa = 3.18PANFNRR934 pKa = 11.84SGNGYY939 pKa = 9.97DD940 pKa = 3.04VDD942 pKa = 5.16GIITCRR948 pKa = 11.84EE949 pKa = 4.14LFEE952 pKa = 4.83EE953 pKa = 4.73MPTNSRR959 pKa = 11.84TTAKK963 pKa = 10.65GFDD966 pKa = 3.42PNFFYY971 pKa = 10.67EE972 pKa = 4.05ALEE975 pKa = 4.33DD976 pKa = 4.26EE977 pKa = 4.79EE978 pKa = 5.82MSMQPLSFYY987 pKa = 10.08PNPVKK992 pKa = 10.62DD993 pKa = 3.61VLTIQTSSFGDD1004 pKa = 3.22EE1005 pKa = 4.07SLQVEE1010 pKa = 5.28VYY1012 pKa = 10.21SVAGVLVYY1020 pKa = 10.26KK1021 pKa = 10.29AAHH1024 pKa = 6.36RR1025 pKa = 11.84ADD1027 pKa = 3.45MHH1029 pKa = 7.59SGEE1032 pKa = 4.6LQVDD1036 pKa = 4.53LQQLRR1041 pKa = 11.84QGVYY1045 pKa = 10.45LLRR1048 pKa = 11.84LQGEE1052 pKa = 4.61YY1053 pKa = 9.74QQQTLKK1059 pKa = 10.44ISKK1062 pKa = 9.31EE1063 pKa = 3.91

Molecular weight: 117.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3LNQ4|A0A0D3LNQ4_9BACT Uncharacterized protein OS=Flammeovirgaceae bacterium 311 OX=1257021 GN=D770_26780 PE=4 SV=1
MM1 pKa = 7.59FFLQLLVFASPRR13 pKa = 11.84GPGGTTNQSQAGSPPKK29 pKa = 10.17RR30 pKa = 11.84GPPRR34 pKa = 11.84GNLVSPRR41 pKa = 11.84IEE43 pKa = 3.52ARR45 pKa = 11.84LRR47 pKa = 11.84QRR49 pKa = 11.84SRR51 pKa = 11.84RR52 pKa = 11.84GTATVALRR60 pKa = 11.84FAAFAKK66 pKa = 10.43AVLLINFSKK75 pKa = 10.71FRR77 pKa = 11.84MTHH80 pKa = 5.76FLVPYY85 pKa = 10.0LSKK88 pKa = 10.69FQGRR92 pKa = 11.84VKK94 pKa = 10.76GAFF97 pKa = 3.2

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5332

0

5332

1897602

33

10634

355.9

39.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.917 ± 0.03

0.743 ± 0.011

5.018 ± 0.026

6.606 ± 0.043

4.543 ± 0.027

7.077 ± 0.038

2.063 ± 0.014

6.242 ± 0.03

5.68 ± 0.037

10.235 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.309 ± 0.015

4.739 ± 0.033

4.177 ± 0.021

4.428 ± 0.03

4.777 ± 0.022

6.236 ± 0.036

5.351 ± 0.037

6.485 ± 0.027

1.34 ± 0.013

4.034 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski