Tissierella creatinophila DSM 6911
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2530 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U7M6B6|A0A1U7M6B6_TISCR Uncharacterized protein OS=Tissierella creatinophila DSM 6911 OX=1123403 GN=TICRE_11350 PE=4 SV=1
MM1 pKa = 7.38 KK2 pKa = 10.51 KK3 pKa = 10.35 EE4 pKa = 4.03 IDD6 pKa = 3.59 NHH8 pKa = 5.18 EE9 pKa = 4.18 HH10 pKa = 7.81 DD11 pKa = 4.62 EE12 pKa = 4.48 NCNHH16 pKa = 6.47 DD17 pKa = 4.2 HH18 pKa = 6.02 EE19 pKa = 6.57 HH20 pKa = 6.06 DD21 pKa = 3.87 HH22 pKa = 6.53 EE23 pKa = 5.14 GEE25 pKa = 3.68 IDD27 pKa = 3.83 VIYY30 pKa = 9.36 LTFEE34 pKa = 4.45 DD35 pKa = 4.9 DD36 pKa = 5.04 EE37 pKa = 4.48 EE38 pKa = 4.91 VEE40 pKa = 4.36 CQVLGVFEE48 pKa = 5.19 VEE50 pKa = 4.1 DD51 pKa = 3.62 KK52 pKa = 11.07 EE53 pKa = 4.63 YY54 pKa = 10.62 IALLPSDD61 pKa = 3.85 EE62 pKa = 5.63 DD63 pKa = 4.03 EE64 pKa = 4.31 ILLYY68 pKa = 10.17 EE69 pKa = 4.41 YY70 pKa = 10.64 KK71 pKa = 10.87 DD72 pKa = 3.4 SDD74 pKa = 4.05 EE75 pKa = 4.66 EE76 pKa = 4.04 FDD78 pKa = 5.77 LIPIEE83 pKa = 4.42 DD84 pKa = 4.12 DD85 pKa = 3.87 KK86 pKa = 11.73 EE87 pKa = 4.47 LEE89 pKa = 4.44 LVSKK93 pKa = 10.65 AYY95 pKa = 10.58 FEE97 pKa = 4.76 LFEE100 pKa = 6.87 DD101 pKa = 4.7 EE102 pKa = 5.89 DD103 pKa = 4.03 DD104 pKa = 4.59 LEE106 pKa = 4.7 YY107 pKa = 11.49 GEE109 pKa = 4.53 YY110 pKa = 10.58 DD111 pKa = 3.48 EE112 pKa = 6.13 EE113 pKa = 4.38 EE114 pKa = 4.1
Molecular weight: 13.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.706
IPC_protein 3.681
Toseland 3.49
ProMoST 3.808
Dawson 3.656
Bjellqvist 3.834
Wikipedia 3.554
Rodwell 3.516
Grimsley 3.401
Solomon 3.643
Lehninger 3.605
Nozaki 3.77
DTASelect 3.923
Thurlkill 3.528
EMBOSS 3.567
Sillero 3.795
Patrickios 0.807
IPC_peptide 3.643
IPC2_peptide 3.783
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A1U7M6G6|A0A1U7M6G6_TISCR Methylglyoxal synthase OS=Tissierella creatinophila DSM 6911 OX=1123403 GN=mgsA PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 8.76 KK9 pKa = 7.46 RR10 pKa = 11.84 QRR12 pKa = 11.84 SRR14 pKa = 11.84 EE15 pKa = 3.65 HH16 pKa = 6.35 GFRR19 pKa = 11.84 KK20 pKa = 10.0 RR21 pKa = 11.84 MRR23 pKa = 11.84 TKK25 pKa = 10.23 SGRR28 pKa = 11.84 SIIKK32 pKa = 9.86 ARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.54 GRR39 pKa = 11.84 KK40 pKa = 8.97 KK41 pKa = 9.66 LTAA44 pKa = 4.2
Molecular weight: 5.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.338
IPC2_protein 10.877
IPC_protein 12.281
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.017
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2530
0
2530
746299
29
2124
295.0
33.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.24 ± 0.055
0.764 ± 0.018
5.979 ± 0.034
8.045 ± 0.051
4.37 ± 0.037
6.571 ± 0.047
1.343 ± 0.02
10.252 ± 0.058
9.109 ± 0.047
9.386 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.644 ± 0.021
5.661 ± 0.04
2.917 ± 0.032
2.286 ± 0.022
3.713 ± 0.035
6.257 ± 0.033
4.922 ± 0.033
6.061 ± 0.04
0.661 ± 0.015
3.817 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here