Gordonia phage Orchid

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Orchidvirus; Gordonia virus Orchid

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 114 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A160DH30|A0A160DH30_9CAUD Portal protein OS=Gordonia phage Orchid OX=1838075 GN=11 PE=4 SV=1
MM1 pKa = 7.12YY2 pKa = 10.65ALTVYY7 pKa = 10.74CDD9 pKa = 3.95DD10 pKa = 4.38GNGKK14 pKa = 9.67YY15 pKa = 9.9VDD17 pKa = 4.35PMSGTYY23 pKa = 9.38SVEE26 pKa = 4.22RR27 pKa = 11.84IGQVITEE34 pKa = 4.3LLISVATDD42 pKa = 3.02VVVSFEE48 pKa = 4.11VSKK51 pKa = 11.22SGEE54 pKa = 3.81

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A160DJ94|A0A160DJ94_9CAUD Uncharacterized protein OS=Gordonia phage Orchid OX=1838075 GN=7 PE=4 SV=1
MM1 pKa = 7.75PKK3 pKa = 9.89KK4 pKa = 10.64CPTPNKK10 pKa = 9.8QRR12 pKa = 11.84FTNKK16 pKa = 9.91YY17 pKa = 6.81EE18 pKa = 4.06ANRR21 pKa = 11.84AISGQWAKK29 pKa = 10.27TGLGPTSTYY38 pKa = 10.52RR39 pKa = 11.84CRR41 pKa = 11.84CGYY44 pKa = 7.25YY45 pKa = 10.17HH46 pKa = 6.61KK47 pKa = 9.53TSSVRR52 pKa = 11.84AKK54 pKa = 9.87PRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 3.4

Molecular weight: 6.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

114

0

114

24095

45

2915

211.4

23.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.666 ± 0.714

0.979 ± 0.145

6.516 ± 0.227

6.151 ± 0.276

3.756 ± 0.149

7.321 ± 0.31

1.756 ± 0.177

6.217 ± 0.144

5.972 ± 0.241

7.039 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.123

5.271 ± 0.106

5.005 ± 0.302

3.569 ± 0.156

4.897 ± 0.213

6.267 ± 0.203

6.375 ± 0.228

6.516 ± 0.312

1.573 ± 0.117

3.465 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski