Amino acid dipepetide frequency for Glaciimonas sp. PCH181

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.363AlaAla: 13.363 ± 0.127
1.132AlaCys: 1.132 ± 0.029
5.714AlaAsp: 5.714 ± 0.066
5.862AlaGlu: 5.862 ± 0.074
3.964AlaPhe: 3.964 ± 0.054
8.366AlaGly: 8.366 ± 0.09
2.321AlaHis: 2.321 ± 0.037
6.735AlaIle: 6.735 ± 0.069
4.566AlaLys: 4.566 ± 0.068
12.503AlaLeu: 12.503 ± 0.114
3.063AlaMet: 3.063 ± 0.05
3.595AlaAsn: 3.595 ± 0.052
4.53AlaPro: 4.53 ± 0.066
4.834AlaGln: 4.834 ± 0.063
5.822AlaArg: 5.822 ± 0.068
6.476AlaSer: 6.476 ± 0.07
6.056AlaThr: 6.056 ± 0.07
7.743AlaVal: 7.743 ± 0.078
1.306AlaTrp: 1.306 ± 0.031
2.585AlaTyr: 2.585 ± 0.04
0.0AlaXaa: 0.0 ± 0.0
Cys
1.077CysAla: 1.077 ± 0.028
0.148CysCys: 0.148 ± 0.012
0.561CysAsp: 0.561 ± 0.019
0.487CysGlu: 0.487 ± 0.018
0.334CysPhe: 0.334 ± 0.014
1.021CysGly: 1.021 ± 0.028
0.261CysHis: 0.261 ± 0.014
0.527CysIle: 0.527 ± 0.02
0.356CysLys: 0.356 ± 0.016
0.894CysLeu: 0.894 ± 0.026
0.229CysMet: 0.229 ± 0.011
0.335CysAsn: 0.335 ± 0.015
0.43CysPro: 0.43 ± 0.019
0.31CysGln: 0.31 ± 0.016
0.482CysArg: 0.482 ± 0.018
0.55CysSer: 0.55 ± 0.021
0.484CysThr: 0.484 ± 0.018
0.716CysVal: 0.716 ± 0.021
0.131CysTrp: 0.131 ± 0.009
0.249CysTyr: 0.249 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.279AspAla: 6.279 ± 0.074
0.482AspCys: 0.482 ± 0.02
2.776AspAsp: 2.776 ± 0.046
2.77AspGlu: 2.77 ± 0.051
2.234AspPhe: 2.234 ± 0.039
4.299AspGly: 4.299 ± 0.057
1.203AspHis: 1.203 ± 0.03
3.276AspIle: 3.276 ± 0.044
2.185AspLys: 2.185 ± 0.04
5.069AspLeu: 5.069 ± 0.064
1.282AspMet: 1.282 ± 0.03
1.696AspAsn: 1.696 ± 0.041
2.543AspPro: 2.543 ± 0.04
1.925AspGln: 1.925 ± 0.04
2.735AspArg: 2.735 ± 0.045
2.565AspSer: 2.565 ± 0.04
2.612AspThr: 2.612 ± 0.044
3.937AspVal: 3.937 ± 0.061
0.83AspTrp: 0.83 ± 0.025
1.557AspTyr: 1.557 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
5.303GluAla: 5.303 ± 0.074
0.447GluCys: 0.447 ± 0.016
2.235GluAsp: 2.235 ± 0.037
2.728GluGlu: 2.728 ± 0.054
1.893GluPhe: 1.893 ± 0.039
3.157GluGly: 3.157 ± 0.052
1.182GluHis: 1.182 ± 0.031
3.48GluIle: 3.48 ± 0.053
2.734GluLys: 2.734 ± 0.053
5.225GluLeu: 5.225 ± 0.069
1.473GluMet: 1.473 ± 0.033
2.021GluAsn: 2.021 ± 0.041
1.817GluPro: 1.817 ± 0.036
2.463GluGln: 2.463 ± 0.043
3.396GluArg: 3.396 ± 0.052
2.621GluSer: 2.621 ± 0.041
2.739GluThr: 2.739 ± 0.044
3.585GluVal: 3.585 ± 0.052
0.749GluTrp: 0.749 ± 0.023
1.294GluTyr: 1.294 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
3.976PheAla: 3.976 ± 0.059
0.459PheCys: 0.459 ± 0.016
2.515PheAsp: 2.515 ± 0.041
1.937PheGlu: 1.937 ± 0.036
1.684PhePhe: 1.684 ± 0.039
3.662PheGly: 3.662 ± 0.061
0.776PheHis: 0.776 ± 0.027
2.209PheIle: 2.209 ± 0.045
1.534PheLys: 1.534 ± 0.033
3.436PheLeu: 3.436 ± 0.049
0.912PheMet: 0.912 ± 0.028
1.499PheAsn: 1.499 ± 0.034
1.72PhePro: 1.72 ± 0.038
1.214PheGln: 1.214 ± 0.035
1.799PheArg: 1.799 ± 0.043
2.954PheSer: 2.954 ± 0.048
2.088PheThr: 2.088 ± 0.036
2.829PheVal: 2.829 ± 0.048
0.542PheTrp: 0.542 ± 0.023
1.139PheTyr: 1.139 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
7.611GlyAla: 7.611 ± 0.079
0.865GlyCys: 0.865 ± 0.028
3.621GlyAsp: 3.621 ± 0.048
3.753GlyGlu: 3.753 ± 0.055
3.371GlyPhe: 3.371 ± 0.048
6.164GlyGly: 6.164 ± 0.103
1.698GlyHis: 1.698 ± 0.036
5.048GlyIle: 5.048 ± 0.062
4.076GlyLys: 4.076 ± 0.057
8.038GlyLeu: 8.038 ± 0.094
2.319GlyMet: 2.319 ± 0.037
2.72GlyAsn: 2.72 ± 0.053
2.503GlyPro: 2.503 ± 0.046
2.895GlyGln: 2.895 ± 0.049
4.078GlyArg: 4.078 ± 0.062
4.451GlySer: 4.451 ± 0.068
4.234GlyThr: 4.234 ± 0.068
5.997GlyVal: 5.997 ± 0.073
1.242GlyTrp: 1.242 ± 0.027
2.398GlyTyr: 2.398 ± 0.041
0.0GlyXaa: 0.0 ± 0.0
His
2.372HisAla: 2.372 ± 0.045
0.28HisCys: 0.28 ± 0.012
1.195HisAsp: 1.195 ± 0.031
0.948HisGlu: 0.948 ± 0.025
1.039HisPhe: 1.039 ± 0.029
1.854HisGly: 1.854 ± 0.037
0.663HisHis: 0.663 ± 0.026
1.272HisIle: 1.272 ± 0.032
0.714HisLys: 0.714 ± 0.02
2.222HisLeu: 2.222 ± 0.043
0.551HisMet: 0.551 ± 0.016
0.67HisAsn: 0.67 ± 0.022
1.401HisPro: 1.401 ± 0.033
0.901HisGln: 0.901 ± 0.023
1.234HisArg: 1.234 ± 0.031
1.148HisSer: 1.148 ± 0.026
1.112HisThr: 1.112 ± 0.028
1.497HisVal: 1.497 ± 0.034
0.361HisTrp: 0.361 ± 0.017
0.694HisTyr: 0.694 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
7.766IleAla: 7.766 ± 0.081
0.624IleCys: 0.624 ± 0.022
3.831IleAsp: 3.831 ± 0.049
3.418IleGlu: 3.418 ± 0.058
2.173IlePhe: 2.173 ± 0.041
5.414IleGly: 5.414 ± 0.078
1.07IleHis: 1.07 ± 0.027
2.872IleIle: 2.872 ± 0.056
2.607IleLys: 2.607 ± 0.042
5.116IleLeu: 5.116 ± 0.065
1.192IleMet: 1.192 ± 0.031
2.329IleAsn: 2.329 ± 0.041
2.783IlePro: 2.783 ± 0.048
1.811IleGln: 1.811 ± 0.037
2.971IleArg: 2.971 ± 0.044
3.867IleSer: 3.867 ± 0.058
3.397IleThr: 3.397 ± 0.053
4.579IleVal: 4.579 ± 0.054
0.653IleTrp: 0.653 ± 0.021
1.406IleTyr: 1.406 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.272LysAla: 4.272 ± 0.067
0.243LysCys: 0.243 ± 0.012
2.148LysAsp: 2.148 ± 0.043
2.112LysGlu: 2.112 ± 0.047
1.379LysPhe: 1.379 ± 0.034
2.7LysGly: 2.7 ± 0.04
0.885LysHis: 0.885 ± 0.024
2.855LysIle: 2.855 ± 0.051
2.293LysLys: 2.293 ± 0.049
4.486LysLeu: 4.486 ± 0.06
1.329LysMet: 1.329 ± 0.031
1.862LysAsn: 1.862 ± 0.038
2.284LysPro: 2.284 ± 0.05
1.746LysGln: 1.746 ± 0.035
2.528LysArg: 2.528 ± 0.046
2.592LysSer: 2.592 ± 0.045
2.716LysThr: 2.716 ± 0.047
3.046LysVal: 3.046 ± 0.053
0.486LysTrp: 0.486 ± 0.02
1.073LysTyr: 1.073 ± 0.027
0.0LysXaa: 0.0 ± 0.0
Leu
12.065LeuAla: 12.065 ± 0.117
1.003LeuCys: 1.003 ± 0.027
5.414LeuAsp: 5.414 ± 0.064
5.096LeuGlu: 5.096 ± 0.062
3.84LeuPhe: 3.84 ± 0.061
7.692LeuGly: 7.692 ± 0.089
2.266LeuHis: 2.266 ± 0.039
5.98LeuIle: 5.98 ± 0.066
4.372LeuLys: 4.372 ± 0.062
10.884LeuLeu: 10.884 ± 0.131
2.636LeuMet: 2.636 ± 0.049
3.515LeuAsn: 3.515 ± 0.051
5.66LeuPro: 5.66 ± 0.073
4.124LeuGln: 4.124 ± 0.058
6.238LeuArg: 6.238 ± 0.074
7.063LeuSer: 7.063 ± 0.084
6.191LeuThr: 6.191 ± 0.062
6.936LeuVal: 6.936 ± 0.074
1.113LeuTrp: 1.113 ± 0.034
2.307LeuTyr: 2.307 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.803MetAla: 2.803 ± 0.048
0.214MetCys: 0.214 ± 0.012
1.139MetAsp: 1.139 ± 0.028
1.132MetGlu: 1.132 ± 0.029
0.855MetPhe: 0.855 ± 0.023
1.799MetGly: 1.799 ± 0.034
0.655MetHis: 0.655 ± 0.023
1.398MetIle: 1.398 ± 0.03
1.195MetLys: 1.195 ± 0.028
2.845MetLeu: 2.845 ± 0.042
0.709MetMet: 0.709 ± 0.024
0.929MetAsn: 0.929 ± 0.025
1.447MetPro: 1.447 ± 0.029
1.255MetGln: 1.255 ± 0.031
1.662MetArg: 1.662 ± 0.025
1.754MetSer: 1.754 ± 0.037
1.842MetThr: 1.842 ± 0.037
1.838MetVal: 1.838 ± 0.035
0.23MetTrp: 0.23 ± 0.013
0.476MetTyr: 0.476 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.966AsnAla: 3.966 ± 0.055
0.355AsnCys: 0.355 ± 0.017
1.766AsnAsp: 1.766 ± 0.032
1.45AsnGlu: 1.45 ± 0.034
1.337AsnPhe: 1.337 ± 0.03
2.961AsnGly: 2.961 ± 0.052
0.709AsnHis: 0.709 ± 0.022
2.078AsnIle: 2.078 ± 0.042
1.385AsnLys: 1.385 ± 0.033
3.519AsnLeu: 3.519 ± 0.046
0.845AsnMet: 0.845 ± 0.023
1.372AsnAsn: 1.372 ± 0.037
2.159AsnPro: 2.159 ± 0.04
1.318AsnGln: 1.318 ± 0.034
1.887AsnArg: 1.887 ± 0.039
1.874AsnSer: 1.874 ± 0.041
2.067AsnThr: 2.067 ± 0.042
2.597AsnVal: 2.597 ± 0.037
0.528AsnTrp: 0.528 ± 0.021
0.952AsnTyr: 0.952 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
5.006ProAla: 5.006 ± 0.067
0.334ProCys: 0.334 ± 0.015
2.816ProAsp: 2.816 ± 0.045
2.948ProGlu: 2.948 ± 0.049
1.84ProPhe: 1.84 ± 0.039
3.401ProGly: 3.401 ± 0.045
1.128ProHis: 1.128 ± 0.029
2.715ProIle: 2.715 ± 0.05
1.834ProLys: 1.834 ± 0.038
4.896ProLeu: 4.896 ± 0.062
1.166ProMet: 1.166 ± 0.027
1.633ProAsn: 1.633 ± 0.034
1.952ProPro: 1.952 ± 0.042
1.959ProGln: 1.959 ± 0.045
2.088ProArg: 2.088 ± 0.039
2.86ProSer: 2.86 ± 0.048
2.815ProThr: 2.815 ± 0.045
3.649ProVal: 3.649 ± 0.057
0.614ProTrp: 0.614 ± 0.018
1.19ProTyr: 1.19 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
4.441GlnAla: 4.441 ± 0.06
0.307GlnCys: 0.307 ± 0.016
1.662GlnAsp: 1.662 ± 0.034
1.803GlnGlu: 1.803 ± 0.036
1.482GlnPhe: 1.482 ± 0.03
2.56GlnGly: 2.56 ± 0.041
1.088GlnHis: 1.088 ± 0.029
2.563GlnIle: 2.563 ± 0.039
1.721GlnLys: 1.721 ± 0.037
4.36GlnLeu: 4.36 ± 0.055
1.072GlnMet: 1.072 ± 0.029
1.327GlnAsn: 1.327 ± 0.033
1.95GlnPro: 1.95 ± 0.039
2.264GlnGln: 2.264 ± 0.043
2.779GlnArg: 2.779 ± 0.046
2.334GlnSer: 2.334 ± 0.038
2.177GlnThr: 2.177 ± 0.037
2.685GlnVal: 2.685 ± 0.045
0.468GlnTrp: 0.468 ± 0.018
0.987GlnTyr: 0.987 ± 0.027
0.0GlnXaa: 0.0 ± 0.0
Arg
5.387ArgAla: 5.387 ± 0.068
0.511ArgCys: 0.511 ± 0.018
3.04ArgAsp: 3.04 ± 0.056
3.12ArgGlu: 3.12 ± 0.055
2.391ArgPhe: 2.391 ± 0.041
3.495ArgGly: 3.495 ± 0.052
1.452ArgHis: 1.452 ± 0.027
3.531ArgIle: 3.531 ± 0.054
2.368ArgLys: 2.368 ± 0.037
6.089ArgLeu: 6.089 ± 0.07
1.563ArgMet: 1.563 ± 0.03
2.021ArgAsn: 2.021 ± 0.043
2.304ArgPro: 2.304 ± 0.034
2.462ArgGln: 2.462 ± 0.038
3.447ArgArg: 3.447 ± 0.053
3.08ArgSer: 3.08 ± 0.053
2.843ArgThr: 2.843 ± 0.044
3.941ArgVal: 3.941 ± 0.053
0.823ArgTrp: 0.823 ± 0.024
1.729ArgTyr: 1.729 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.77SerAla: 6.77 ± 0.078
0.524SerCys: 0.524 ± 0.022
3.187SerAsp: 3.187 ± 0.052
2.887SerGlu: 2.887 ± 0.045
2.361SerPhe: 2.361 ± 0.043
5.539SerGly: 5.539 ± 0.07
1.323SerHis: 1.323 ± 0.029
3.444SerIle: 3.444 ± 0.048
2.406SerLys: 2.406 ± 0.044
6.104SerLeu: 6.104 ± 0.08
1.547SerMet: 1.547 ± 0.03
2.025SerAsn: 2.025 ± 0.048
2.842SerPro: 2.842 ± 0.04
2.138SerGln: 2.138 ± 0.037
3.11SerArg: 3.11 ± 0.05
3.895SerSer: 3.895 ± 0.057
3.429SerThr: 3.429 ± 0.052
4.337SerVal: 4.337 ± 0.055
0.808SerTrp: 0.808 ± 0.025
1.582SerTyr: 1.582 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
6.024ThrAla: 6.024 ± 0.077
0.464ThrCys: 0.464 ± 0.018
2.859ThrAsp: 2.859 ± 0.045
2.687ThrGlu: 2.687 ± 0.044
2.215ThrPhe: 2.215 ± 0.035
4.657ThrGly: 4.657 ± 0.083
1.172ThrHis: 1.172 ± 0.029
3.33ThrIle: 3.33 ± 0.05
2.024ThrLys: 2.024 ± 0.044
6.677ThrLeu: 6.677 ± 0.063
1.315ThrMet: 1.315 ± 0.031
1.815ThrAsn: 1.815 ± 0.043
3.415ThrPro: 3.415 ± 0.052
2.086ThrGln: 2.086 ± 0.041
2.869ThrArg: 2.869 ± 0.044
3.361ThrSer: 3.361 ± 0.053
3.309ThrThr: 3.309 ± 0.059
4.235ThrVal: 4.235 ± 0.064
0.622ThrTrp: 0.622 ± 0.021
1.386ThrTyr: 1.386 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
8.273ValAla: 8.273 ± 0.084
0.752ValCys: 0.752 ± 0.025
3.803ValAsp: 3.803 ± 0.055
3.718ValGlu: 3.718 ± 0.059
2.739ValPhe: 2.739 ± 0.047
5.406ValGly: 5.406 ± 0.058
1.369ValHis: 1.369 ± 0.028
4.59ValIle: 4.59 ± 0.059
3.103ValLys: 3.103 ± 0.052
7.45ValLeu: 7.45 ± 0.084
2.034ValMet: 2.034 ± 0.041
2.512ValAsn: 2.512 ± 0.045
3.291ValPro: 3.291 ± 0.047
2.478ValGln: 2.478 ± 0.04
3.917ValArg: 3.917 ± 0.06
4.468ValSer: 4.468 ± 0.057
4.397ValThr: 4.397 ± 0.064
5.72ValVal: 5.72 ± 0.075
0.871ValTrp: 0.871 ± 0.023
1.598ValTyr: 1.598 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.04TrpAla: 1.04 ± 0.026
0.153TrpCys: 0.153 ± 0.01
0.603TrpAsp: 0.603 ± 0.022
0.553TrpGlu: 0.553 ± 0.019
0.549TrpPhe: 0.549 ± 0.02
0.803TrpGly: 0.803 ± 0.026
0.364TrpHis: 0.364 ± 0.018
0.782TrpIle: 0.782 ± 0.029
0.551TrpLys: 0.551 ± 0.021
1.76TrpLeu: 1.76 ± 0.041
0.404TrpMet: 0.404 ± 0.016
0.465TrpAsn: 0.465 ± 0.02
0.522TrpPro: 0.522 ± 0.018
0.692TrpGln: 0.692 ± 0.025
0.913TrpArg: 0.913 ± 0.026
0.776TrpSer: 0.776 ± 0.026
0.666TrpThr: 0.666 ± 0.023
0.848TrpVal: 0.848 ± 0.025
0.209TrpTrp: 0.209 ± 0.013
0.31TrpTyr: 0.31 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.743TyrAla: 2.743 ± 0.045
0.287TyrCys: 0.287 ± 0.015
1.371TyrAsp: 1.371 ± 0.034
1.131TyrGlu: 1.131 ± 0.03
1.204TyrPhe: 1.204 ± 0.028
2.129TyrGly: 2.129 ± 0.039
0.542TyrHis: 0.542 ± 0.019
1.195TyrIle: 1.195 ± 0.033
1.013TyrLys: 1.013 ± 0.025
2.822TyrLeu: 2.822 ± 0.048
0.536TyrMet: 0.536 ± 0.022
0.782TyrAsn: 0.782 ± 0.022
1.291TyrPro: 1.291 ± 0.028
1.155TyrGln: 1.155 ± 0.031
1.645TyrArg: 1.645 ± 0.035
1.569TyrSer: 1.569 ± 0.035
1.348TyrThr: 1.348 ± 0.03
1.774TyrVal: 1.774 ± 0.034
0.376TyrTrp: 0.376 ± 0.018
0.757TyrTyr: 0.757 ± 0.023
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4634 proteins (1480305 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski