Candidatus Bathyarchaeota archaeon
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2329 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U0RXR1|A0A2U0RXR1_9ARCH Uncharacterized protein OS=Candidatus Bathyarchaeota archaeon OX=2026714 GN=CW691_09435 PE=4 SV=1
MM1 pKa = 7.46 QINKK5 pKa = 9.61 KK6 pKa = 10.3 LSTGLIVTLLALSTIAIAIPSAFAVVANLQAYY38 pKa = 6.49 TAMGAPITGGTVGQSIRR55 pKa = 11.84 VGGDD59 pKa = 2.87 SAAAWALVQFYY70 pKa = 10.02 WDD72 pKa = 3.61 SPANKK77 pKa = 9.1 IGEE80 pKa = 4.35 VYY82 pKa = 10.99 ADD84 pKa = 3.61 GAGDD88 pKa = 3.87 FLLTGITVPEE98 pKa = 4.27 DD99 pKa = 3.16 VAGNHH104 pKa = 6.09 QILVNDD110 pKa = 3.8 GTGNVALNSNINPKK124 pKa = 8.99 LTISATKK131 pKa = 10.22 ALPGDD136 pKa = 3.94 SLAVTGSGFGDD147 pKa = 3.75 EE148 pKa = 4.66 VPVGIYY154 pKa = 10.27 LGTITSVVTEE164 pKa = 4.55 AVADD168 pKa = 4.26 DD169 pKa = 5.39 DD170 pKa = 5.61 LDD172 pKa = 3.79 EE173 pKa = 5.23 SPVVKK178 pKa = 9.62 GTVVLTVDD186 pKa = 3.34 VTVDD190 pKa = 3.18 GDD192 pKa = 3.46 IGGTAVTGPATLTVTDD208 pKa = 4.62 DD209 pKa = 4.11 GEE211 pKa = 4.73 GVLSGTLSDD220 pKa = 3.89 VVVDD224 pKa = 4.2 DD225 pKa = 4.41 GVVVEE230 pKa = 5.06 DD231 pKa = 4.74 GEE233 pKa = 4.43 VDD235 pKa = 3.51 VTIVGTINYY244 pKa = 7.16 ATGGIALSASGVDD257 pKa = 3.64 SAAGTGTVLNIAVTIDD273 pKa = 4.32 AIDD276 pKa = 3.49 AAYY279 pKa = 10.18 QYY281 pKa = 11.11 AQYY284 pKa = 10.85 EE285 pKa = 4.38 VTPLGGITTSAAGSFAEE302 pKa = 4.85 SILVPAIPVLSYY314 pKa = 11.7 GNYY317 pKa = 10.02 LDD319 pKa = 4.17 TAIDD323 pKa = 3.8 TDD325 pKa = 4.28 GNTANPTPVASLSVDD340 pKa = 3.24 YY341 pKa = 10.8 YY342 pKa = 10.76 ILVNPISGPAGITVTLSGRR361 pKa = 11.84 IPANTVYY368 pKa = 10.51 EE369 pKa = 4.13 IRR371 pKa = 11.84 LDD373 pKa = 3.55 TTTIATGTSAADD385 pKa = 3.28 TTYY388 pKa = 10.0 TQTHH392 pKa = 6.02 QISSFLSKK400 pKa = 9.3 TAHH403 pKa = 5.52 TFYY406 pKa = 11.16 VVWGGTNTRR415 pKa = 11.84 SATFTVTEE423 pKa = 4.3 SPRR426 pKa = 11.84 MALSSTSGMAGQVITISSVPGYY448 pKa = 9.11 PFSAGADD455 pKa = 3.22 LTLYY459 pKa = 10.7 INGAVVNGTTIDD471 pKa = 3.71 DD472 pKa = 3.83 RR473 pKa = 11.84 FGPTVAFGPASGTFTDD489 pKa = 5.38 LEE491 pKa = 4.26 FTIPALAPGVYY502 pKa = 10.06 AIEE505 pKa = 4.76 LVDD508 pKa = 4.22 SYY510 pKa = 11.67 GASTGTMYY518 pKa = 10.73 TFTVVPTPEE527 pKa = 4.19 TAVSMVGTVFYY538 pKa = 10.63 PGDD541 pKa = 3.43 TLAWSFSSTDD551 pKa = 3.41 TVTVAPTVTVRR562 pKa = 11.84 SPTGAVWWQGVWATTTSGPMTTVLYY587 pKa = 10.6 QNQLFGGAHH596 pKa = 5.79 ATLPSNAPLGSWNWTAVYY614 pKa = 9.13 STGSAANVKK623 pKa = 8.85 ATGLFSVMAAATLDD637 pKa = 3.7 TVTTDD642 pKa = 3.41 IADD645 pKa = 4.05 LVDD648 pKa = 5.54 DD649 pKa = 5.02 ISDD652 pKa = 3.45 ISDD655 pKa = 3.53 DD656 pKa = 3.61 VATINTNINNLEE668 pKa = 4.07 DD669 pKa = 5.57 LIDD672 pKa = 4.09 ALEE675 pKa = 4.23 VTVDD679 pKa = 3.26 IPEE682 pKa = 4.46 LTTLTNDD689 pKa = 3.09 VASLKK694 pKa = 10.54 VSVSALDD701 pKa = 3.69 AVVTAVAGDD710 pKa = 3.75 VATVDD715 pKa = 3.76 TKK717 pKa = 10.87 IGTLTGTITGIEE729 pKa = 4.15 GNIATIEE736 pKa = 4.17 TDD738 pKa = 3.23 VGTLQADD745 pKa = 3.48 ISDD748 pKa = 3.82 VEE750 pKa = 4.67 ANVDD754 pKa = 3.73 STPAWIAVVLALVAAVAAIFAVITIRR780 pKa = 11.84 QKK782 pKa = 10.44 IAGG785 pKa = 3.82
Molecular weight: 79.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.554
IPC_protein 3.617
Toseland 3.376
ProMoST 3.795
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.579
Rodwell 3.427
Grimsley 3.274
Solomon 3.617
Lehninger 3.567
Nozaki 3.732
DTASelect 4.024
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.732
Patrickios 0.896
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.699
Protein with the highest isoelectric point:
>tr|A0A2U0RZ30|A0A2U0RZ30_9ARCH Aspartate aminotransferase family protein OS=Candidatus Bathyarchaeota archaeon OX=2026714 GN=CW691_07835 PE=3 SV=1
MM1 pKa = 7.81 RR2 pKa = 11.84 LAKK5 pKa = 10.19 AGKK8 pKa = 8.64 EE9 pKa = 3.98 KK10 pKa = 10.84 KK11 pKa = 9.46 PVPTWVIAKK20 pKa = 8.97 TGGQVRR26 pKa = 11.84 SNPKK30 pKa = 8.73 RR31 pKa = 11.84 RR32 pKa = 11.84 RR33 pKa = 11.84 WRR35 pKa = 11.84 QRR37 pKa = 11.84 KK38 pKa = 8.35 IRR40 pKa = 11.84 AA41 pKa = 3.38
Molecular weight: 4.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.31
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.983
Sillero 12.501
Patrickios 12.047
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.053
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2329
0
2329
642103
25
1519
275.7
30.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.205 ± 0.047
1.285 ± 0.025
5.192 ± 0.046
6.588 ± 0.051
4.191 ± 0.039
6.954 ± 0.055
1.691 ± 0.023
7.18 ± 0.049
6.925 ± 0.074
9.303 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.359 ± 0.024
4.462 ± 0.048
4.03 ± 0.031
3.071 ± 0.029
4.093 ± 0.043
6.475 ± 0.049
6.387 ± 0.061
7.878 ± 0.044
1.268 ± 0.025
3.462 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here