Sierra dome spider associated circular virus 2
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPD5|A0A346BPD5_9VIRU Putative capsid protein OS=Sierra dome spider associated circular virus 2 OX=2293300 PE=4 SV=1
MM1 pKa = 7.56 CLDD4 pKa = 4.71 LLTVSNMSRR13 pKa = 11.84 CRR15 pKa = 11.84 NYY17 pKa = 10.3 VFTLNNPQGTPMFVPDD33 pKa = 3.14 KK34 pKa = 8.99 MKK36 pKa = 10.95 YY37 pKa = 8.59 MVYY40 pKa = 10.1 QKK42 pKa = 10.93 EE43 pKa = 4.25 SGEE46 pKa = 4.18 NGTNHH51 pKa = 4.87 YY52 pKa = 9.79 QGYY55 pKa = 9.53 VEE57 pKa = 5.06 LLSPTGMPSVKK68 pKa = 10.19 RR69 pKa = 11.84 MIGQNPHH76 pKa = 6.43 LEE78 pKa = 4.22 PRR80 pKa = 11.84 RR81 pKa = 11.84 GTQAEE86 pKa = 3.78 ARR88 pKa = 11.84 AYY90 pKa = 10.29 CMKK93 pKa = 10.5 PEE95 pKa = 3.97 TRR97 pKa = 11.84 VDD99 pKa = 4.19 GPWEE103 pKa = 3.9 MGTFTEE109 pKa = 4.17 NNQGKK114 pKa = 7.88 RR115 pKa = 11.84 TDD117 pKa = 4.03 LEE119 pKa = 4.26 LAIQDD124 pKa = 3.41 IDD126 pKa = 4.17 GGSSMARR133 pKa = 11.84 VALDD137 pKa = 3.36 HH138 pKa = 6.82 PVTFVKK144 pKa = 10.58 YY145 pKa = 9.76 PRR147 pKa = 11.84 GLSAYY152 pKa = 10.18 ANMRR156 pKa = 11.84 NFRR159 pKa = 11.84 GTTYY163 pKa = 9.87 TKK165 pKa = 10.28 KK166 pKa = 10.57 QSIVLWGSPGCGKK179 pKa = 9.18 TSTLVNYY186 pKa = 9.62 FPDD189 pKa = 4.32 MYY191 pKa = 11.72 VKK193 pKa = 10.1 MCDD196 pKa = 3.36 NKK198 pKa = 9.88 WWDD201 pKa = 3.84 GYY203 pKa = 11.31 SDD205 pKa = 3.67 QKK207 pKa = 11.23 QVLLDD212 pKa = 4.47 DD213 pKa = 4.8 FPNTYY218 pKa = 7.96 TAKK221 pKa = 8.63 QTKK224 pKa = 9.93 DD225 pKa = 3.27 LLGEE229 pKa = 4.21 LDD231 pKa = 4.59 LPRR234 pKa = 11.84 EE235 pKa = 4.35 SKK237 pKa = 10.92 GQVIKK242 pKa = 10.7 PRR244 pKa = 11.84 VEE246 pKa = 4.04 FFFITSNFHH255 pKa = 5.53 PDD257 pKa = 2.63 EE258 pKa = 4.28 WFEE261 pKa = 4.29 SAKK264 pKa = 10.18 QQDD267 pKa = 3.51 RR268 pKa = 11.84 LAVRR272 pKa = 11.84 RR273 pKa = 11.84 RR274 pKa = 11.84 ITKK277 pKa = 8.85 TYY279 pKa = 9.51 NFDD282 pKa = 3.4 VEE284 pKa = 4.18 EE285 pKa = 4.68 EE286 pKa = 3.32 IWEE289 pKa = 4.34 FVRR292 pKa = 11.84 EE293 pKa = 3.95 FSPYY297 pKa = 9.78 NVRR300 pKa = 11.84 TQEE303 pKa = 3.88 LL304 pKa = 3.73
Molecular weight: 35.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.658
IPC2_protein 6.839
IPC_protein 6.985
Toseland 6.751
ProMoST 7.41
Dawson 7.6
Bjellqvist 7.585
Wikipedia 7.527
Rodwell 7.6
Grimsley 6.795
Solomon 7.688
Lehninger 7.717
Nozaki 7.849
DTASelect 7.717
Thurlkill 7.761
EMBOSS 7.849
Sillero 8.024
Patrickios 4.228
IPC_peptide 7.688
IPC2_peptide 7.102
IPC2.peptide.svr19 7.274
Protein with the highest isoelectric point:
>tr|A0A346BPD5|A0A346BPD5_9VIRU Putative capsid protein OS=Sierra dome spider associated circular virus 2 OX=2293300 PE=4 SV=1
MM1 pKa = 7.16 YY2 pKa = 10.36 GRR4 pKa = 11.84 RR5 pKa = 11.84 SYY7 pKa = 10.89 RR8 pKa = 11.84 RR9 pKa = 11.84 YY10 pKa = 7.66 RR11 pKa = 11.84 TYY13 pKa = 10.96 RR14 pKa = 11.84 KK15 pKa = 8.91 GSGRR19 pKa = 11.84 ARR21 pKa = 11.84 YY22 pKa = 8.39 GATKK26 pKa = 9.64 ARR28 pKa = 11.84 RR29 pKa = 11.84 VVRR32 pKa = 11.84 YY33 pKa = 9.06 RR34 pKa = 11.84 GARR37 pKa = 11.84 ARR39 pKa = 11.84 GIKK42 pKa = 10.09 RR43 pKa = 11.84 IVNRR47 pKa = 11.84 QIEE50 pKa = 4.61 TKK52 pKa = 9.76 EE53 pKa = 4.05 YY54 pKa = 10.14 TNGTQTSMLQPTQLGAFGAGYY75 pKa = 10.48 NGFSDD80 pKa = 3.59 TVSYY84 pKa = 8.6 FKK86 pKa = 11.11 PMDD89 pKa = 3.82 YY90 pKa = 10.73 LSQGTSRR97 pKa = 11.84 NTRR100 pKa = 11.84 IGDD103 pKa = 3.39 AVTIMSIEE111 pKa = 3.53 WRR113 pKa = 11.84 YY114 pKa = 10.72 CITFTDD120 pKa = 4.24 YY121 pKa = 9.8 EE122 pKa = 4.33 QEE124 pKa = 4.58 FIRR127 pKa = 11.84 VMAFTAPFGFTPTYY141 pKa = 11.07 GNIFEE146 pKa = 5.4 APDD149 pKa = 3.51 GNVDD153 pKa = 3.54 VRR155 pKa = 11.84 VARR158 pKa = 11.84 PNTNITVLKK167 pKa = 9.91 QYY169 pKa = 11.0 KK170 pKa = 8.39 HH171 pKa = 5.58 TLSPLNSATASSSSTVNTPQVFRR194 pKa = 11.84 TGCLRR199 pKa = 11.84 LLFPGGRR206 pKa = 11.84 KK207 pKa = 8.43 FQYY210 pKa = 10.67 SFNNYY215 pKa = 8.42 TGSTALFNRR224 pKa = 11.84 NRR226 pKa = 11.84 DD227 pKa = 3.2 IFIVAVPFAAKK238 pKa = 10.14 RR239 pKa = 11.84 LQGSTLTQYY248 pKa = 10.43 TFIQGQEE255 pKa = 3.91 LAVKK259 pKa = 10.57 FKK261 pKa = 11.03 DD262 pKa = 3.33 AA263 pKa = 4.38
Molecular weight: 29.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.306
IPC2_protein 10.028
IPC_protein 10.789
Toseland 10.438
ProMoST 10.292
Dawson 10.657
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.804
Grimsley 10.76
Solomon 10.73
Lehninger 10.672
Nozaki 10.423
DTASelect 10.423
Thurlkill 10.511
EMBOSS 10.862
Sillero 10.57
Patrickios 10.35
IPC_peptide 10.716
IPC2_peptide 9.458
IPC2.peptide.svr19 8.179
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
567
263
304
283.5
32.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.467 ± 1.329
1.235 ± 0.295
4.586 ± 0.96
4.762 ± 1.306
5.82 ± 0.637
7.407 ± 0.596
0.882 ± 0.312
3.704 ± 0.771
5.291 ± 0.689
5.996 ± 0.655
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.175 ± 0.792
5.467 ± 0.09
4.938 ± 0.706
4.938 ± 0.003
8.466 ± 1.356
5.82 ± 0.4
8.995 ± 1.027
6.173 ± 0.292
1.235 ± 0.531
5.644 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here