Capybara microvirus Cap3_SP_613
Average proteome isoelectric point is 5.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W569|A0A4P8W569_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_613 OX=2585483 PE=3 SV=1
MM1 pKa = 7.58 LKK3 pKa = 10.79 LNLRR7 pKa = 11.84 EE8 pKa = 3.88 NFIYY12 pKa = 10.58 EE13 pKa = 4.28 SIDD16 pKa = 3.39 YY17 pKa = 10.45 SKK19 pKa = 11.51 GSIVDD24 pKa = 3.68 TYY26 pKa = 11.55 KK27 pKa = 11.13 VILPYY32 pKa = 10.68 QKK34 pKa = 10.43 DD35 pKa = 3.72 DD36 pKa = 3.85 KK37 pKa = 11.62 GVFLNDD43 pKa = 3.3 SDD45 pKa = 3.9 TPIYY49 pKa = 10.42 VKK51 pKa = 10.43 SGKK54 pKa = 10.07 KK55 pKa = 10.16 NLDD58 pKa = 3.24 EE59 pKa = 4.93 YY60 pKa = 11.13 INSFKK65 pKa = 10.93 DD66 pKa = 2.82 EE67 pKa = 4.11 CDD69 pKa = 2.83 IYY71 pKa = 11.33 NILSKK76 pKa = 10.97 FEE78 pKa = 4.13 STGDD82 pKa = 3.56 VSSLNLGRR90 pKa = 11.84 GVYY93 pKa = 10.65 ADD95 pKa = 3.35 ISKK98 pKa = 10.57 IPDD101 pKa = 3.97 NIHH104 pKa = 7.23 DD105 pKa = 4.55 FNNALNQGAEE115 pKa = 4.13 ILNTLDD121 pKa = 3.02 SDD123 pKa = 3.72 LRR125 pKa = 11.84 KK126 pKa = 10.07 AILEE130 pKa = 3.9 NDD132 pKa = 2.79 IQAINKK138 pKa = 6.94 ATNNILNGSNKK149 pKa = 9.53 EE150 pKa = 4.02 VLQDD154 pKa = 3.55 EE155 pKa = 4.97 SIKK158 pKa = 10.65 EE159 pKa = 3.91 AA160 pKa = 3.86
Molecular weight: 18.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.58
IPC2_protein 4.546
IPC_protein 4.469
Toseland 4.291
ProMoST 4.609
Dawson 4.444
Bjellqvist 4.596
Wikipedia 4.355
Rodwell 4.304
Grimsley 4.19
Solomon 4.444
Lehninger 4.393
Nozaki 4.546
DTASelect 4.774
Thurlkill 4.304
EMBOSS 4.368
Sillero 4.584
Patrickios 3.948
IPC_peptide 4.444
IPC2_peptide 4.571
IPC2.peptide.svr19 4.544
Protein with the highest isoelectric point:
>tr|A0A4P8W5S5|A0A4P8W5S5_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_613 OX=2585483 PE=4 SV=1
MM1 pKa = 7.31 TCLNLSYY8 pKa = 10.69 VPCIKK13 pKa = 10.63 YY14 pKa = 10.58 KK15 pKa = 10.99 NSDD18 pKa = 3.33 KK19 pKa = 11.32 KK20 pKa = 11.47 KK21 pKa = 8.8 MLFKK25 pKa = 10.87 KK26 pKa = 10.42 KK27 pKa = 10.15 ILVKK31 pKa = 10.72 NKK33 pKa = 10.08 SDD35 pKa = 4.44 LDD37 pKa = 3.96 LKK39 pKa = 10.46 IDD41 pKa = 3.97 KK42 pKa = 9.54 EE43 pKa = 4.41 NCFLEE48 pKa = 4.77 LKK50 pKa = 10.18 EE51 pKa = 4.27 SSNLFDD57 pKa = 3.83 SVEE60 pKa = 4.01 FLQVPCGICKK70 pKa = 10.31 EE71 pKa = 4.15 CLKK74 pKa = 11.04 KK75 pKa = 10.36 NARR78 pKa = 11.84 DD79 pKa = 3.12 WAFRR83 pKa = 11.84 CVKK86 pKa = 9.93 EE87 pKa = 3.78 ASQYY91 pKa = 10.2 KK92 pKa = 8.09 EE93 pKa = 3.83 NYY95 pKa = 8.73 MITLTYY101 pKa = 11.18 DD102 pKa = 3.33 NDD104 pKa = 3.61 NLPFNGSLVKK114 pKa = 10.92 DD115 pKa = 4.42 EE116 pKa = 4.06 ISKK119 pKa = 10.5 FNKK122 pKa = 9.61 KK123 pKa = 10.03 LKK125 pKa = 9.77 TYY127 pKa = 10.69 LSRR130 pKa = 11.84 LNLPSDD136 pKa = 3.6 FRR138 pKa = 11.84 FFGCGEE144 pKa = 4.01 YY145 pKa = 10.69 GSQFGRR151 pKa = 11.84 PHH153 pKa = 4.51 YY154 pKa = 10.34 HH155 pKa = 6.47 IIYY158 pKa = 10.56 FNLQLSKK165 pKa = 10.9 LSEE168 pKa = 4.25 LLGLRR173 pKa = 11.84 YY174 pKa = 9.75 YY175 pKa = 10.88 GKK177 pKa = 10.04 SSKK180 pKa = 10.49 GFPIYY185 pKa = 10.35 SCKK188 pKa = 10.4 FLDD191 pKa = 4.06 DD192 pKa = 3.52 TWSKK196 pKa = 11.38 GIVKK200 pKa = 10.07 VSEE203 pKa = 4.16 CDD205 pKa = 3.03 IGSCAYY211 pKa = 9.3 VAHH214 pKa = 6.19 YY215 pKa = 10.34 VDD217 pKa = 4.73 KK218 pKa = 10.87 KK219 pKa = 10.49 KK220 pKa = 10.98 CFTKK224 pKa = 9.98 GQKK227 pKa = 9.68 EE228 pKa = 3.74 FFYY231 pKa = 10.97 NNTGLVPDD239 pKa = 4.36 FCVMSRR245 pKa = 11.84 NPGIGSCFIEE255 pKa = 5.06 DD256 pKa = 3.32 VDD258 pKa = 4.49 NNFLKK263 pKa = 11.04 GFFNAYY269 pKa = 10.72 LNGQCYY275 pKa = 10.14 AIPKK279 pKa = 9.75 FYY281 pKa = 10.38 INKK284 pKa = 9.73 LKK286 pKa = 10.72 DD287 pKa = 3.59 YY288 pKa = 10.58 GLTSYY293 pKa = 10.99 DD294 pKa = 3.07 IYY296 pKa = 11.33 KK297 pKa = 10.99 DD298 pKa = 3.45 NLLSNRR304 pKa = 11.84 FNIDD308 pKa = 3.05 SYY310 pKa = 11.69 FLDD313 pKa = 3.56 IEE315 pKa = 4.57 KK316 pKa = 10.78 GFSLNYY322 pKa = 9.96 NLSNNKK328 pKa = 8.62 GRR330 pKa = 11.84 CFF332 pKa = 3.45
Molecular weight: 38.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.045
IPC2_protein 8.126
IPC_protein 7.922
Toseland 7.658
ProMoST 8.507
Dawson 8.726
Bjellqvist 8.96
Wikipedia 8.697
Rodwell 8.785
Grimsley 7.585
Solomon 8.785
Lehninger 8.785
Nozaki 9.151
DTASelect 8.726
Thurlkill 8.799
EMBOSS 8.902
Sillero 9.077
Patrickios 3.884
IPC_peptide 8.77
IPC2_peptide 7.805
IPC2.peptide.svr19 8.072
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1394
70
571
232.3
26.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.524 ± 1.622
1.865 ± 0.803
6.456 ± 0.942
4.304 ± 0.32
6.6 ± 0.923
5.452 ± 0.833
1.22 ± 0.44
6.6 ± 1.071
6.887 ± 1.848
9.469 ± 0.381
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.578 ± 0.321
9.684 ± 1.018
3.156 ± 0.973
3.156 ± 0.689
2.941 ± 0.393
9.828 ± 0.951
4.161 ± 0.686
4.878 ± 0.395
0.717 ± 0.257
5.524 ± 0.548
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here