Vibrio virus Vf33

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Villovirus

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7DKP6|Q7DKP6_9VIRU Vpf77 OS=Vibrio virus Vf33 OX=127509 GN=vpf77 PE=4 SV=1
MM1 pKa = 6.89NHH3 pKa = 6.09YY4 pKa = 10.66LRR6 pKa = 11.84FFIALVILCASSHH19 pKa = 5.59TYY21 pKa = 10.64ALEE24 pKa = 3.73ARR26 pKa = 11.84IGYY29 pKa = 7.73MQMRR33 pKa = 11.84GCGSQGDD40 pKa = 3.71WVDD43 pKa = 3.54PYY45 pKa = 11.42KK46 pKa = 11.44VNTCFLDD53 pKa = 2.96TGYY56 pKa = 10.96FDD58 pKa = 4.08SCTFEE63 pKa = 3.84KK64 pKa = 10.36TPYY67 pKa = 10.25SDD69 pKa = 4.43ARR71 pKa = 11.84YY72 pKa = 8.79PYY74 pKa = 9.28QTVCDD79 pKa = 3.93NGLGLAYY86 pKa = 10.81YY87 pKa = 8.88EE88 pKa = 4.36VRR90 pKa = 11.84CPEE93 pKa = 3.81NSEE96 pKa = 4.27FDD98 pKa = 3.99PSTLRR103 pKa = 11.84CKK105 pKa = 10.33SVCEE109 pKa = 4.04YY110 pKa = 11.13GKK112 pKa = 10.26NPDD115 pKa = 3.94GTCMDD120 pKa = 3.88ACQFKK125 pKa = 10.9KK126 pKa = 10.85SIDD129 pKa = 3.7EE130 pKa = 4.31TKK132 pKa = 10.41LLQWVAYY139 pKa = 9.99VYY141 pKa = 10.92GEE143 pKa = 4.26QVTGACYY150 pKa = 10.82GDD152 pKa = 4.99FGATRR157 pKa = 11.84CEE159 pKa = 4.24LGRR162 pKa = 11.84VPSDD166 pKa = 3.07TTLCTDD172 pKa = 3.63VEE174 pKa = 4.7SGQWTQNTLCHH185 pKa = 6.58GNFQFTGNQCEE196 pKa = 4.28GGTLFWGKK204 pKa = 10.11DD205 pKa = 3.62GPDD208 pKa = 3.31TPIIPDD214 pKa = 4.37DD215 pKa = 5.14PIHH218 pKa = 7.61DD219 pKa = 4.88PDD221 pKa = 5.88DD222 pKa = 3.89PTGDD226 pKa = 3.49IEE228 pKa = 5.08DD229 pKa = 4.6PSVLPDD235 pKa = 3.61GSTNTVNPPDD245 pKa = 4.02TEE247 pKa = 4.11KK248 pKa = 11.1KK249 pKa = 10.17PDD251 pKa = 3.59VEE253 pKa = 6.26DD254 pKa = 5.31PDD256 pKa = 4.5TDD258 pKa = 4.12DD259 pKa = 3.73STDD262 pKa = 3.46MAVLNAIKK270 pKa = 10.53GLNSDD275 pKa = 3.77VNKK278 pKa = 10.59ALNDD282 pKa = 3.47MNIDD286 pKa = 3.46INQASADD293 pKa = 3.72VQNQIIALNASMVTNTQAIQKK314 pKa = 7.84QQINDD319 pKa = 3.14NKK321 pKa = 10.37IYY323 pKa = 10.49EE324 pKa = 4.16NTKK327 pKa = 10.95ALIQQANADD336 pKa = 3.12ITTAVNKK343 pKa = 7.74NTNAINGVGDD353 pKa = 4.15DD354 pKa = 3.78VEE356 pKa = 5.55KK357 pKa = 10.74IAGAMDD363 pKa = 5.45GIAEE367 pKa = 4.26DD368 pKa = 3.65VSGISDD374 pKa = 3.9TLDD377 pKa = 4.1GIANTDD383 pKa = 3.08TSGAGTGGTCIEE395 pKa = 4.51SQTCTGFYY403 pKa = 10.47EE404 pKa = 4.29SAYY407 pKa = 9.62PDD409 pKa = 3.66GLGGLVSGQLDD420 pKa = 3.78SLKK423 pKa = 11.03HH424 pKa = 4.14NTIDD428 pKa = 3.61NFVSSFGDD436 pKa = 3.42LDD438 pKa = 3.84LSSAKK443 pKa = 10.1RR444 pKa = 11.84PSFVLPVPFFGDD456 pKa = 3.76FSFEE460 pKa = 4.02EE461 pKa = 4.39QISFDD466 pKa = 3.32WVFGFIRR473 pKa = 11.84AVLIMTSVFAARR485 pKa = 11.84RR486 pKa = 11.84IIFGGG491 pKa = 3.39

Molecular weight: 53.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7DKP7|Q7DKP7_9VIRU Vpf81 OS=Vibrio virus Vf33 OX=127509 GN=vpf81 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.4RR3 pKa = 11.84LNALKK8 pKa = 10.66KK9 pKa = 10.26FGKK12 pKa = 9.38QAAATVTVAVLSVPAMAAEE31 pKa = 5.12GGAADD36 pKa = 4.47PFSAIDD42 pKa = 3.74LSGVATKK49 pKa = 10.3IGAAGLVIVGITMAYY64 pKa = 9.95KK65 pKa = 10.65SITLAKK71 pKa = 9.96RR72 pKa = 11.84AVNKK76 pKa = 10.37AA77 pKa = 3.05

Molecular weight: 7.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1652

77

491

236.0

26.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.809 ± 1.576

2.119 ± 0.516

7.324 ± 1.021

4.661 ± 0.576

4.661 ± 0.303

7.203 ± 0.594

1.695 ± 0.535

6.235 ± 0.395

5.569 ± 0.849

8.232 ± 1.11

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.24 ± 0.613

4.116 ± 0.697

4.782 ± 0.288

3.753 ± 0.454

4.237 ± 0.864

5.932 ± 0.244

6.78 ± 0.493

6.901 ± 0.691

1.695 ± 0.332

4.056 ± 0.565
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski