Oscillibacter sp. CAG:241
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1823 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R6HG37|R6HG37_9FIRM Histidine kinase OS=Oscillibacter sp. CAG:241 OX=1262911 GN=BN557_01341 PE=4 SV=1
MM1 pKa = 7.53 KK2 pKa = 10.22 KK3 pKa = 10.56 LSRR6 pKa = 11.84 LAVMLALVLALTACSGGADD25 pKa = 3.55 TPSPDD30 pKa = 3.34 EE31 pKa = 4.06 QTEE34 pKa = 4.3 GQHH37 pKa = 6.05 PVTDD41 pKa = 3.71 AAEE44 pKa = 5.12 LEE46 pKa = 4.59 GQWTSPSGNILSFSADD62 pKa = 3.09 QGRR65 pKa = 11.84 YY66 pKa = 6.99 TYY68 pKa = 9.21 QTFSGRR74 pKa = 11.84 TGQGPYY80 pKa = 10.2 DD81 pKa = 3.7 AAADD85 pKa = 3.8 VPTIDD90 pKa = 4.97 FDD92 pKa = 3.78 GFLYY96 pKa = 10.75 DD97 pKa = 6.16 FILRR101 pKa = 11.84 DD102 pKa = 4.57 DD103 pKa = 4.69 GVLLPNQNGSGGDD116 pKa = 3.57 AEE118 pKa = 5.32 SIDD121 pKa = 3.47 HH122 pKa = 5.16 FTFRR126 pKa = 11.84 RR127 pKa = 11.84 SSDD130 pKa = 2.87 ARR132 pKa = 11.84 IDD134 pKa = 3.4 LWEE137 pKa = 4.6 LSDD140 pKa = 6.31 LSGVWQNALGEE151 pKa = 4.39 TLVIDD156 pKa = 4.9 ADD158 pKa = 3.81 RR159 pKa = 11.84 MAYY162 pKa = 10.49 LSYY165 pKa = 10.57 TRR167 pKa = 11.84 LSMGSGTIGDD177 pKa = 4.17 DD178 pKa = 3.47 QDD180 pKa = 4.02 GKK182 pKa = 11.44 GPYY185 pKa = 9.91 LAMNGRR191 pKa = 11.84 IYY193 pKa = 10.83 LSPGLDD199 pKa = 3.06 KK200 pKa = 11.42 DD201 pKa = 3.75 RR202 pKa = 11.84 FTLLGQDD209 pKa = 3.3 ADD211 pKa = 4.07 GSFSGVFYY219 pKa = 10.04 PYY221 pKa = 10.45 GQAAAYY227 pKa = 6.76 TALPGASFEE236 pKa = 4.22 TDD238 pKa = 2.57 EE239 pKa = 5.9 DD240 pKa = 5.9 GYY242 pKa = 10.99 CRR244 pKa = 11.84 WYY246 pKa = 11.14 SDD248 pKa = 3.04 GHH250 pKa = 5.89 DD251 pKa = 3.28 AFYY254 pKa = 9.58 ITGSYY259 pKa = 8.89 TLAEE263 pKa = 5.13 DD264 pKa = 3.74 GLAYY268 pKa = 10.34 RR269 pKa = 11.84 DD270 pKa = 4.4 DD271 pKa = 4.59 GPVYY275 pKa = 10.57 AAGFDD280 pKa = 4.49 PIPYY284 pKa = 10.11 DD285 pKa = 3.92 PAEE288 pKa = 4.46 DD289 pKa = 3.91 WGDD292 pKa = 2.87 SWMSNWGG299 pKa = 3.36
Molecular weight: 32.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.617
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.012
Wikipedia 3.821
Rodwell 3.668
Grimsley 3.528
Solomon 3.859
Lehninger 3.821
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.681
EMBOSS 3.834
Sillero 3.986
Patrickios 1.011
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.851
Protein with the highest isoelectric point:
>tr|R6GU42|R6GU42_9FIRM Uncharacterized protein OS=Oscillibacter sp. CAG:241 OX=1262911 GN=BN557_01473 PE=4 SV=1
MM1 pKa = 7.89 DD2 pKa = 4.0 KK3 pKa = 10.01 WKK5 pKa = 9.21 KK6 pKa = 9.18 TIMHH10 pKa = 6.53 MGAYY14 pKa = 8.22 PAALGKK20 pKa = 9.24 WVLLGGIIGVAGGVIGSLFHH40 pKa = 8.19 IGVNYY45 pKa = 8.4 ATAIRR50 pKa = 11.84 GAHH53 pKa = 5.93 PWILYY58 pKa = 7.62 LLPVGGLAIVGLYY71 pKa = 9.65 KK72 pKa = 10.54 LCHH75 pKa = 6.72 LEE77 pKa = 4.27 GKK79 pKa = 8.16 GTNAIIEE86 pKa = 4.44 SVHH89 pKa = 6.84 FGEE92 pKa = 5.05 SVPILLVPVIFVSTVITHH110 pKa = 6.78 LCGGSAGRR118 pKa = 11.84 EE119 pKa = 3.93 GAALQIGGGLGFQAGKK135 pKa = 10.35 LLRR138 pKa = 11.84 LGEE141 pKa = 4.34 KK142 pKa = 10.13 DD143 pKa = 4.13 LPLATLCGMSGVFSALFGTPLTATVFALEE172 pKa = 4.38 VISVGVLYY180 pKa = 10.84 YY181 pKa = 10.89 AGLVPCITAAMAAFGVSALMGVEE204 pKa = 3.98 PTRR207 pKa = 11.84 FTVSMPRR214 pKa = 11.84 VTLEE218 pKa = 3.48 LMLPVVVLSILCALVSILFCKK239 pKa = 9.44 GLHH242 pKa = 4.59 WTEE245 pKa = 4.12 HH246 pKa = 5.85 LLTRR250 pKa = 11.84 SFRR253 pKa = 11.84 NPWLRR258 pKa = 11.84 VLAGAAILIVLSLLTNGDD276 pKa = 3.78 YY277 pKa = 11.23 NGAGMDD283 pKa = 3.86 VIARR287 pKa = 11.84 ALRR290 pKa = 11.84 GNVSGWAWLGEE301 pKa = 4.21 VLFFPLPPLLGAVHH315 pKa = 6.67 RR316 pKa = 11.84 RR317 pKa = 11.84 HH318 pKa = 6.27 HH319 pKa = 6.47 RR320 pKa = 11.84 LRR322 pKa = 11.84 LQGRR326 pKa = 11.84 RR327 pKa = 11.84 GGAVVLRR334 pKa = 11.84 GRR336 pKa = 11.84 CLRR339 pKa = 11.84 LLHH342 pKa = 6.62 GGAPGPACGLCRR354 pKa = 11.84 GHH356 pKa = 6.92 RR357 pKa = 11.84 PGGGVLRR364 pKa = 11.84 GGEE367 pKa = 4.12 LPRR370 pKa = 11.84 GVGAAQRR377 pKa = 11.84 GAVRR381 pKa = 11.84 LCWGALLRR389 pKa = 11.84 RR390 pKa = 11.84 GLRR393 pKa = 11.84 PEE395 pKa = 4.59 LSPLRR400 pKa = 11.84 LLRR403 pKa = 11.84 PVQQPDD409 pKa = 3.25 HH410 pKa = 7.12 PLFQAPCGVHH420 pKa = 5.56 QRR422 pKa = 11.84 PHH424 pKa = 5.71 PRR426 pKa = 11.84 VTKK429 pKa = 9.66 MM430 pKa = 3.37
Molecular weight: 45.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.354
IPC2_protein 9.604
IPC_protein 10.482
Toseland 10.555
ProMoST 10.321
Dawson 10.687
Bjellqvist 10.452
Wikipedia 10.906
Rodwell 10.804
Grimsley 10.745
Solomon 10.804
Lehninger 10.76
Nozaki 10.613
DTASelect 10.423
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.628
Patrickios 10.482
IPC_peptide 10.804
IPC2_peptide 9.838
IPC2.peptide.svr19 8.475
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1823
0
1823
571517
29
2348
313.5
34.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.834 ± 0.074
1.744 ± 0.031
5.832 ± 0.043
6.104 ± 0.062
3.469 ± 0.042
8.206 ± 0.105
1.993 ± 0.044
5.291 ± 0.048
4.265 ± 0.057
10.175 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.837 ± 0.032
3.044 ± 0.04
4.233 ± 0.038
3.815 ± 0.04
6.394 ± 0.075
5.209 ± 0.05
5.531 ± 0.059
7.502 ± 0.062
1.042 ± 0.024
3.478 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here