Streptomyces sp. 769

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9427 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8ERP4|A0A0A8ERP4_9ACTN Uncharacterized protein OS=Streptomyces sp. 769 OX=1262452 GN=GZL_04164 PE=4 SV=1
MM1 pKa = 7.83PEE3 pKa = 4.78DD4 pKa = 4.38LDD6 pKa = 4.91HH7 pKa = 7.87PEE9 pKa = 4.05QLWARR14 pKa = 11.84AATLAVVAAAMNDD27 pKa = 2.93GDD29 pKa = 4.31EE30 pKa = 4.42YY31 pKa = 11.26SWGPGGLACWNCGGSYY47 pKa = 7.22WWRR50 pKa = 11.84LKK52 pKa = 10.96LYY54 pKa = 10.57DD55 pKa = 4.71DD56 pKa = 5.29GRR58 pKa = 11.84ALLCGQDD65 pKa = 3.09SDD67 pKa = 4.79GSYY70 pKa = 8.53THH72 pKa = 6.84SGDD75 pKa = 3.44KK76 pKa = 10.89QIDD79 pKa = 3.8FLAGGPAWLPWEE91 pKa = 4.11QLRR94 pKa = 11.84DD95 pKa = 3.75DD96 pKa = 4.15AQGNLLGFAYY106 pKa = 9.38WYY108 pKa = 10.15EE109 pKa = 4.33DD110 pKa = 3.93GVWARR115 pKa = 11.84APYY118 pKa = 8.75PATMPDD124 pKa = 4.14DD125 pKa = 4.45GLEE128 pKa = 4.1MALGWAAPGDD138 pKa = 3.59AAVRR142 pKa = 11.84EE143 pKa = 4.45ITEE146 pKa = 4.06HH147 pKa = 6.64LVALTEE153 pKa = 4.36TDD155 pKa = 3.29VHH157 pKa = 5.9PAEE160 pKa = 4.31TVRR163 pKa = 11.84AFIASAAARR172 pKa = 11.84TVGAADD178 pKa = 3.53VFALLDD184 pKa = 3.86AVCGPDD190 pKa = 2.53CWYY193 pKa = 10.37EE194 pKa = 4.07VRR196 pKa = 11.84PEE198 pKa = 3.64AALAFAADD206 pKa = 4.27LGLTGDD212 pKa = 4.09RR213 pKa = 11.84GAVPAVASS221 pKa = 3.82

Molecular weight: 23.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8ELB6|A0A0A8ELB6_9ACTN Type II/IV secretion system protein OS=Streptomyces sp. 769 OX=1262452 GN=GZL_03666 PE=3 SV=1
MM1 pKa = 7.28SRR3 pKa = 11.84VGLVSRR9 pKa = 11.84VGRR12 pKa = 11.84AGGVSAGPPPRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84SAARR30 pKa = 11.84RR31 pKa = 11.84AARR34 pKa = 11.84TT35 pKa = 3.46

Molecular weight: 3.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9427

0

9427

2889423

29

11132

306.5

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.077 ± 0.043

0.863 ± 0.008

5.874 ± 0.021

5.389 ± 0.025

2.648 ± 0.014

9.539 ± 0.026

2.538 ± 0.014

3.056 ± 0.019

1.976 ± 0.024

10.413 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.737 ± 0.012

1.67 ± 0.014

6.439 ± 0.028

2.871 ± 0.016

8.397 ± 0.031

4.82 ± 0.019

6.146 ± 0.025

8.098 ± 0.024

1.506 ± 0.012

1.943 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski