Mucilaginibacter sp. PPCGB 2223

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Mucilaginibacter; unclassified Mucilaginibacter

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4665 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C2GM64|A0A1C2GM64_9SPHI Uncharacterized protein OS=Mucilaginibacter sp. PPCGB 2223 OX=1886027 GN=BEL04_13705 PE=4 SV=1
MM1 pKa = 7.12STITLKK7 pKa = 11.11GSFDD11 pKa = 3.75TPGTNGVLIEE21 pKa = 4.46VYY23 pKa = 10.23HH24 pKa = 6.35PNPNGYY30 pKa = 9.85DD31 pKa = 3.5YY32 pKa = 11.52KK33 pKa = 11.23KK34 pKa = 10.98DD35 pKa = 3.52FDD37 pKa = 5.49DD38 pKa = 5.12DD39 pKa = 4.89FNVTLTDD46 pKa = 4.04LKK48 pKa = 11.08AGEE51 pKa = 4.45TYY53 pKa = 10.94YY54 pKa = 11.34VDD56 pKa = 4.6LSGSTTSKK64 pKa = 9.01FTLVIDD70 pKa = 3.95GDD72 pKa = 3.75IDD74 pKa = 4.11GSISLPYY81 pKa = 9.92TGFFIDD87 pKa = 4.85GFTFSTLL94 pKa = 3.27

Molecular weight: 10.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C2GTK1|A0A1C2GTK1_9SPHI Pseudouridylate synthase OS=Mucilaginibacter sp. PPCGB 2223 OX=1886027 GN=BEL04_05620 PE=4 SV=1
MM1 pKa = 7.38FIIRR5 pKa = 11.84AFRR8 pKa = 11.84LPCTTGPVICVPAGRR23 pKa = 11.84PIKK26 pKa = 10.47KK27 pKa = 10.06GVFHH31 pKa = 7.02LPVLKK36 pKa = 10.64CSAALCYY43 pKa = 9.84HH44 pKa = 6.99LKK46 pKa = 10.3KK47 pKa = 10.83AMRR50 pKa = 11.84HH51 pKa = 4.71FKK53 pKa = 10.61AYY55 pKa = 10.76LLTVRR60 pKa = 11.84EE61 pKa = 4.18AKK63 pKa = 10.1KK64 pKa = 10.46RR65 pKa = 11.84IYY67 pKa = 10.88SNNFSAGNRR76 pKa = 3.64

Molecular weight: 8.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4665

0

4665

1631383

52

3419

349.7

39.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.983 ± 0.033

0.841 ± 0.011

5.54 ± 0.027

5.049 ± 0.039

4.749 ± 0.029

7.062 ± 0.036

1.971 ± 0.018

7.077 ± 0.035

6.669 ± 0.039

9.197 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.28 ± 0.018

5.582 ± 0.03

4.09 ± 0.022

3.793 ± 0.022

3.883 ± 0.024

6.104 ± 0.031

6.191 ± 0.053

6.485 ± 0.029

1.207 ± 0.015

4.248 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski