Capreolus capreolus papillomavirus 1
Average proteome isoelectric point is 6.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B3ST97|B3ST97_9PAPI Major capsid protein L1 OS=Capreolus capreolus papillomavirus 1 OX=470214 GN=L1 PE=3 SV=1
MM1 pKa = 7.74 PPLKK5 pKa = 9.92 RR6 pKa = 11.84 VKK8 pKa = 10.12 RR9 pKa = 11.84 ANVYY13 pKa = 10.56 DD14 pKa = 4.31 LYY16 pKa = 10.21 RR17 pKa = 11.84 TCKK20 pKa = 9.53 QAGTCPADD28 pKa = 3.55 VIPKK32 pKa = 9.85 VEE34 pKa = 4.18 GKK36 pKa = 8.14 TVADD40 pKa = 4.93 KK41 pKa = 10.72 ILQYY45 pKa = 11.24 GSLGVYY51 pKa = 10.45 LGGLGIGTGMGKK63 pKa = 9.95 APPGGYY69 pKa = 9.67 VPLRR73 pKa = 11.84 GGGSSASLPSNPFAGGIPLEE93 pKa = 4.39 SLEE96 pKa = 4.4 TWGSFRR102 pKa = 11.84 PGIIEE107 pKa = 4.06 DD108 pKa = 3.83 AGPLVEE114 pKa = 4.69 GTVPDD119 pKa = 4.17 APAVIIPEE127 pKa = 4.23 SVPVDD132 pKa = 3.55 EE133 pKa = 5.46 GVSGLDD139 pKa = 3.26 VSSDD143 pKa = 2.78 ISTDD147 pKa = 3.25 TAITFLSAEE156 pKa = 4.22 GADD159 pKa = 4.3 DD160 pKa = 3.69 IAVLEE165 pKa = 4.21 VRR167 pKa = 11.84 PTEE170 pKa = 3.96 HH171 pKa = 6.54 LQPHH175 pKa = 5.53 VMSRR179 pKa = 11.84 SNFQNPLYY187 pKa = 9.92 QPPVRR192 pKa = 11.84 HH193 pKa = 6.22 ASVIADD199 pKa = 3.45 TSGSEE204 pKa = 4.07 NILVGGTGVGSQSGEE219 pKa = 4.22 SIEE222 pKa = 5.08 LSLFNGPRR230 pKa = 11.84 TSTPAEE236 pKa = 3.94 PFQGRR241 pKa = 11.84 KK242 pKa = 9.67 GLFNWFSKK250 pKa = 10.3 RR251 pKa = 11.84 YY252 pKa = 5.78 YY253 pKa = 8.32 TQVPVEE259 pKa = 4.88 DD260 pKa = 4.44 PDD262 pKa = 4.14 EE263 pKa = 4.1 IAAAGSYY270 pKa = 9.73 VFEE273 pKa = 4.51 NPVYY277 pKa = 10.31 DD278 pKa = 3.79 SKK280 pKa = 11.6 AYY282 pKa = 9.77 KK283 pKa = 10.11 PEE285 pKa = 3.91 QQLDD289 pKa = 3.83 TLQPAQEE296 pKa = 4.21 SEE298 pKa = 4.33 YY299 pKa = 10.45 TSAARR304 pKa = 11.84 ILQGPSGRR312 pKa = 11.84 IGVSRR317 pKa = 11.84 VSRR320 pKa = 11.84 PTSIGTRR327 pKa = 11.84 SGVRR331 pKa = 11.84 VGPLYY336 pKa = 10.52 HH337 pKa = 6.65 LRR339 pKa = 11.84 HH340 pKa = 6.04 SFSTIDD346 pKa = 3.55 EE347 pKa = 4.44 PVSLEE352 pKa = 4.09 LLPLITEE359 pKa = 4.35 DD360 pKa = 4.44 SEE362 pKa = 4.79 VLTSVTEE369 pKa = 3.96 AHH371 pKa = 6.85 LGTEE375 pKa = 4.15 DD376 pKa = 5.18 FEE378 pKa = 7.41 DD379 pKa = 3.81 IDD381 pKa = 5.18 LEE383 pKa = 4.6 SFDD386 pKa = 5.36 SDD388 pKa = 3.28 MTLFEE393 pKa = 4.56 STDD396 pKa = 3.12 HH397 pKa = 6.99 HH398 pKa = 6.79 ISQGAGGRR406 pKa = 11.84 RR407 pKa = 11.84 RR408 pKa = 11.84 LLLPVTHH415 pKa = 6.68 TKK417 pKa = 10.59 SLGVGAISSSAQGQGIAYY435 pKa = 8.91 EE436 pKa = 3.96 DD437 pKa = 3.57 TEE439 pKa = 4.49 YY440 pKa = 11.45 VNEE443 pKa = 4.24 NGGHH447 pKa = 6.38 GSTPTVVIDD456 pKa = 4.24 GNINLQEE463 pKa = 3.98 VLYY466 pKa = 10.0 RR467 pKa = 11.84 QYY469 pKa = 11.19 FLHH472 pKa = 6.91 PSLMKK477 pKa = 10.12 RR478 pKa = 11.84 KK479 pKa = 9.3 RR480 pKa = 11.84 RR481 pKa = 11.84 RR482 pKa = 11.84 KK483 pKa = 9.67 RR484 pKa = 11.84 LFGG487 pKa = 3.91
Molecular weight: 52.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.039
IPC2_protein 5.067
IPC_protein 5.029
Toseland 4.991
ProMoST 5.169
Dawson 5.029
Bjellqvist 5.156
Wikipedia 4.889
Rodwell 4.94
Grimsley 4.914
Solomon 5.029
Lehninger 4.978
Nozaki 5.156
DTASelect 5.296
Thurlkill 4.991
EMBOSS 4.952
Sillero 5.219
Patrickios 4.024
IPC_peptide 5.029
IPC2_peptide 5.219
IPC2.peptide.svr19 5.159
Protein with the highest isoelectric point:
>tr|B3ST95|B3ST95_9PAPI E5 OS=Capreolus capreolus papillomavirus 1 OX=470214 PE=4 SV=1
MM1 pKa = 7.27 ATMCFTHH8 pKa = 7.04 LLVHH12 pKa = 7.0 PLTLDD17 pKa = 3.46 TTLKK21 pKa = 10.85 ACGNTTTTQCQGGPTRR37 pKa = 11.84 PTEE40 pKa = 4.08 PSLTLLLDD48 pKa = 3.76 TLPFTVPSEE57 pKa = 3.97 LAKK60 pKa = 10.29 TGIGPQVARR69 pKa = 11.84 LPTHH73 pKa = 5.61 LTPRR77 pKa = 11.84 GVLLAAIPPPRR88 pKa = 11.84 CRR90 pKa = 11.84 ARR92 pKa = 11.84 YY93 pKa = 8.3 RR94 pKa = 11.84 WVINEE99 pKa = 3.48 GRR101 pKa = 11.84 KK102 pKa = 8.01 ATRR105 pKa = 11.84 HH106 pKa = 4.78 HH107 pKa = 6.52 HH108 pKa = 4.9 QTPQKK113 pKa = 10.73
Molecular weight: 12.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.311
IPC_protein 9.619
Toseland 10.423
ProMoST 10.145
Dawson 10.511
Bjellqvist 10.204
Wikipedia 10.657
Rodwell 10.804
Grimsley 10.54
Solomon 10.599
Lehninger 10.584
Nozaki 10.496
DTASelect 10.16
Thurlkill 10.423
EMBOSS 10.804
Sillero 10.467
Patrickios 10.643
IPC_peptide 10.613
IPC2_peptide 9.589
IPC2.peptide.svr19 8.311
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2414
46
612
301.8
33.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.628 ± 0.591
2.527 ± 0.751
5.178 ± 0.374
5.468 ± 0.323
4.101 ± 0.416
7.457 ± 0.818
2.486 ± 0.325
3.48 ± 0.392
4.722 ± 0.569
9.486 ± 0.65
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.864 ± 0.249
3.563 ± 0.468
6.545 ± 0.789
4.432 ± 0.382
5.965 ± 0.449
8.119 ± 0.994
6.794 ± 0.7
6.545 ± 0.371
1.574 ± 0.358
3.065 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here