Merida virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Merhavirus; Merida merhavirus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140DDD9|A0A140DDD9_9RHAB Matrix protein OS=Merida virus OX=1803034 PE=4 SV=1
MM1 pKa = 7.45SNSGGEE7 pKa = 4.09SDD9 pKa = 4.32HH10 pKa = 5.53TTKK13 pKa = 10.65SQEE16 pKa = 3.85DD17 pKa = 3.53SRR19 pKa = 11.84IQGFYY24 pKa = 10.87SEE26 pKa = 5.07GVNLPQEE33 pKa = 4.5SVSCLGALLEE43 pKa = 4.08YY44 pKa = 10.6DD45 pKa = 3.71QEE47 pKa = 4.41GLEE50 pKa = 4.33KK51 pKa = 10.25TFSQMDD57 pKa = 3.88LEE59 pKa = 5.3DD60 pKa = 4.45PLTGEE65 pKa = 4.32RR66 pKa = 11.84EE67 pKa = 4.28GGSVQVSVPWGTVSEE82 pKa = 4.29EE83 pKa = 3.8QQAEE87 pKa = 4.3YY88 pKa = 10.98LRR90 pKa = 11.84LLRR93 pKa = 11.84LYY95 pKa = 9.87NIPHH99 pKa = 7.66PDD101 pKa = 3.44TPEE104 pKa = 3.75EE105 pKa = 4.02DD106 pKa = 3.26TGVDD110 pKa = 3.52EE111 pKa = 6.45DD112 pKa = 4.6EE113 pKa = 5.23LSSGTPSLPPTSPSLSSEE131 pKa = 3.93GGDD134 pKa = 3.15RR135 pKa = 11.84PLSPFLFSDD144 pKa = 3.94EE145 pKa = 4.85EE146 pKa = 4.07YY147 pKa = 10.74TEE149 pKa = 4.33YY150 pKa = 11.59VDD152 pKa = 3.11ITVPEE157 pKa = 4.12EE158 pKa = 4.04LQDD161 pKa = 3.61VALRR165 pKa = 11.84TPLISLLRR173 pKa = 11.84DD174 pKa = 3.4VVRR177 pKa = 11.84IMVNSKK183 pKa = 10.55NEE185 pKa = 3.98PFYY188 pKa = 11.33SLQKK192 pKa = 10.47YY193 pKa = 8.48DD194 pKa = 4.05LKK196 pKa = 11.12QGTVRR201 pKa = 11.84LARR204 pKa = 11.84VGNLYY209 pKa = 9.34TYY211 pKa = 10.09CPEE214 pKa = 4.07GTPIPVLEE222 pKa = 4.17QRR224 pKa = 11.84GSPKK228 pKa = 8.99TRR230 pKa = 11.84KK231 pKa = 9.27KK232 pKa = 10.55RR233 pKa = 11.84SLSTPQTNPTEE244 pKa = 4.69SSAQGPTSSGSRR256 pKa = 11.84ADD258 pKa = 3.71PTPSCSTTKK267 pKa = 10.45TDD269 pKa = 3.62IALGQTNQVSQTTEE283 pKa = 4.04EE284 pKa = 4.34SWPSPDD290 pKa = 4.37HH291 pKa = 6.84SGSPSNLEE299 pKa = 3.51GSVTIKK305 pKa = 10.46RR306 pKa = 11.84AASPSSSSHH315 pKa = 5.31YY316 pKa = 10.24SQTSSVEE323 pKa = 4.01SSSEE327 pKa = 3.81DD328 pKa = 3.37EE329 pKa = 4.46EE330 pKa = 4.61EE331 pKa = 4.31IEE333 pKa = 4.53VAQEE337 pKa = 4.52SYY339 pKa = 11.73SFMCPIYY346 pKa = 10.19GSSEE350 pKa = 3.75MSVKK354 pKa = 9.97VFPGHH359 pKa = 5.42EE360 pKa = 4.19VEE362 pKa = 5.12PLMKK366 pKa = 10.29LGMVPPEE373 pKa = 3.75IFRR376 pKa = 11.84AILRR380 pKa = 11.84DD381 pKa = 3.55RR382 pKa = 11.84GLLRR386 pKa = 11.84ALEE389 pKa = 4.2AVLDD393 pKa = 3.7IGQTHH398 pKa = 5.3MTT400 pKa = 3.99

Molecular weight: 43.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140DDE0|A0A140DDE0_9RHAB Glycoprotein OS=Merida virus OX=1803034 PE=4 SV=1
MM1 pKa = 7.41SFVSMLKK8 pKa = 10.47GKK10 pKa = 9.26PGPSKK15 pKa = 10.43RR16 pKa = 11.84SPSTGHH22 pKa = 6.3VPFQGRR28 pKa = 11.84LDD30 pKa = 3.51MSLTIFTDD38 pKa = 3.33KK39 pKa = 10.27PYY41 pKa = 11.4SSMEE45 pKa = 3.8EE46 pKa = 3.96AFLHH50 pKa = 6.56LIEE53 pKa = 4.43IQDD56 pKa = 4.29YY57 pKa = 10.47ISEE60 pKa = 4.18TRR62 pKa = 11.84DD63 pKa = 3.34LKK65 pKa = 11.16GLYY68 pKa = 9.66LGLIAISILFSKK80 pKa = 10.67CDD82 pKa = 3.36ARR84 pKa = 11.84GNGVMYY90 pKa = 10.84TMDD93 pKa = 4.16LKK95 pKa = 11.33DD96 pKa = 4.11LVSLISTDD104 pKa = 3.13SALASGPLRR113 pKa = 11.84TFSYY117 pKa = 10.71EE118 pKa = 3.71RR119 pKa = 11.84AHH121 pKa = 6.97DD122 pKa = 4.12LRR124 pKa = 11.84GKK126 pKa = 7.5TALVRR131 pKa = 11.84FQLNFEE137 pKa = 4.16QSGLEE142 pKa = 4.18CEE144 pKa = 4.56SLLSVLLKK152 pKa = 10.02RR153 pKa = 11.84DD154 pKa = 3.23KK155 pKa = 11.07NKK157 pKa = 10.71EE158 pKa = 4.03VMSSSTSYY166 pKa = 11.12LKK168 pKa = 10.58LWRR171 pKa = 11.84LKK173 pKa = 10.39MIDD176 pKa = 3.84YY177 pKa = 10.71KK178 pKa = 10.92RR179 pKa = 11.84GTLFSLSS186 pKa = 3.5

Molecular weight: 20.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3708

186

2136

741.6

83.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.421 ± 0.487

1.591 ± 0.233

5.556 ± 0.372

6.311 ± 0.79

4.342 ± 0.707

7.012 ± 0.082

2.131 ± 0.145

5.771 ± 0.587

5.556 ± 0.642

10.086 ± 0.731

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.292 ± 0.287

3.209 ± 0.371

5.07 ± 0.713

3.344 ± 0.272

5.933 ± 0.458

9.736 ± 1.003

5.771 ± 0.56

5.69 ± 0.398

1.888 ± 0.387

3.29 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski