Primate bocaparvovirus 1 (strain Human bocavirus 1 type 1) (HBoV1) (Human bocavirus type 1)
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q3YPH4-2|CAPSD-2_HBOC1 Isoform of Q3YPH4 Isoform Minor capsid protein VP2 of Minor capsid protein VP1 OS=Primate bocaparvovirus 1 (strain Human bocavirus 1 type 1) OX=689403 GN=VP1 PE=1 SV=1
MM1 pKa = 7.91 AYY3 pKa = 10.11 GGRR6 pKa = 11.84 GPWEE10 pKa = 4.18 HH11 pKa = 7.08 LPEE14 pKa = 5.53 DD15 pKa = 4.03 FTLHH19 pKa = 5.9 EE20 pKa = 4.77 NGYY23 pKa = 8.48 CTDD26 pKa = 3.57 CGGYY30 pKa = 10.16 LPHH33 pKa = 7.0 SADD36 pKa = 2.72 NSMYY40 pKa = 10.02 TDD42 pKa = 3.68 RR43 pKa = 11.84 ASEE46 pKa = 4.15 TSTGDD51 pKa = 3.04 ITPSDD56 pKa = 4.17 LGDD59 pKa = 3.58 SDD61 pKa = 6.05 GEE63 pKa = 4.19 DD64 pKa = 3.79 TEE66 pKa = 5.34 PEE68 pKa = 4.16 TSQVDD73 pKa = 3.39 YY74 pKa = 10.99 CPPKK78 pKa = 10.12 KK79 pKa = 10.01 RR80 pKa = 11.84 RR81 pKa = 11.84 LTAPASPPNSPASSVSTITFFNTWHH106 pKa = 6.07 AQPRR110 pKa = 11.84 DD111 pKa = 3.47 EE112 pKa = 5.66 DD113 pKa = 3.57 EE114 pKa = 3.96 LRR116 pKa = 11.84 EE117 pKa = 4.07 YY118 pKa = 10.51 EE119 pKa = 4.19 RR120 pKa = 11.84 QASLLQKK127 pKa = 10.19 KK128 pKa = 9.63 RR129 pKa = 11.84 EE130 pKa = 3.91 SRR132 pKa = 11.84 KK133 pKa = 9.77 RR134 pKa = 11.84 GEE136 pKa = 4.28 EE137 pKa = 3.49 EE138 pKa = 3.76 TLADD142 pKa = 3.92 NSSQEE147 pKa = 4.32 QEE149 pKa = 4.2 PQPDD153 pKa = 3.54 PTQWGEE159 pKa = 3.83 RR160 pKa = 11.84 LGFISSGTPNQPPIVLHH177 pKa = 6.12 CFEE180 pKa = 5.58 DD181 pKa = 4.13 LRR183 pKa = 11.84 PSDD186 pKa = 4.13 EE187 pKa = 5.84 DD188 pKa = 3.13 EE189 pKa = 4.5 GEE191 pKa = 4.28 YY192 pKa = 10.11 IGEE195 pKa = 4.13 KK196 pKa = 10.01 RR197 pKa = 11.84 QQ198 pKa = 3.24
Molecular weight: 22.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.446
IPC2_protein 4.431
IPC_protein 4.368
Toseland 4.202
ProMoST 4.469
Dawson 4.317
Bjellqvist 4.469
Wikipedia 4.177
Rodwell 4.202
Grimsley 4.113
Solomon 4.317
Lehninger 4.266
Nozaki 4.418
DTASelect 4.571
Thurlkill 4.215
EMBOSS 4.202
Sillero 4.482
Patrickios 3.656
IPC_peptide 4.317
IPC2_peptide 4.469
IPC2.peptide.svr19 4.402
Protein with the highest isoelectric point:
>sp|D0EZM8-2|NS1-2_HBOC1 Isoform of D0EZM8 Isoform NS1-70 of Initiator protein NS1 OS=Primate bocaparvovirus 1 (strain Human bocavirus 1 type 1) OX=689403 GN=NS1 PE=1 SV=1
MM1 pKa = 7.65 SSGNMKK7 pKa = 10.39 DD8 pKa = 2.71 KK9 pKa = 10.78 HH10 pKa = 6.39 RR11 pKa = 11.84 SYY13 pKa = 10.78 KK14 pKa = 10.43 RR15 pKa = 11.84 KK16 pKa = 9.64 GSPEE20 pKa = 3.37 RR21 pKa = 11.84 GEE23 pKa = 4.79 RR24 pKa = 11.84 KK25 pKa = 9.5 RR26 pKa = 11.84 HH27 pKa = 4.27 WQTTHH32 pKa = 5.44 HH33 pKa = 7.14 RR34 pKa = 11.84 SRR36 pKa = 11.84 SRR38 pKa = 11.84 SPIRR42 pKa = 11.84 HH43 pKa = 5.29 SGEE46 pKa = 4.01 RR47 pKa = 11.84 GSGSYY52 pKa = 9.62 HH53 pKa = 5.13 QEE55 pKa = 3.68 HH56 pKa = 7.52 PISHH60 pKa = 7.22 LSSCTASKK68 pKa = 9.97 TSDD71 pKa = 2.93 QVMKK75 pKa = 10.19 TRR77 pKa = 11.84 EE78 pKa = 4.1 STSGKK83 pKa = 9.7 KK84 pKa = 10.0 DD85 pKa = 3.23 NRR87 pKa = 11.84 TNPYY91 pKa = 9.31 TVFSQHH97 pKa = 6.31 RR98 pKa = 11.84 ASNPEE103 pKa = 3.64 APGWCGFYY111 pKa = 9.51 WHH113 pKa = 6.33 STRR116 pKa = 11.84 IARR119 pKa = 11.84 DD120 pKa = 3.46 GTNSIFNEE128 pKa = 4.27 MKK130 pKa = 10.4 QQFQQLQIDD139 pKa = 4.28 NKK141 pKa = 10.42 IGWDD145 pKa = 3.43 NTRR148 pKa = 11.84 EE149 pKa = 3.89 LLFNQKK155 pKa = 8.12 KK156 pKa = 7.62 TLDD159 pKa = 3.31 QKK161 pKa = 9.62 YY162 pKa = 10.72 RR163 pKa = 11.84 NMFWHH168 pKa = 6.58 FRR170 pKa = 11.84 NNSDD174 pKa = 5.36 CEE176 pKa = 4.02 RR177 pKa = 11.84 CNYY180 pKa = 8.91 WDD182 pKa = 3.44 DD183 pKa = 3.41 VYY185 pKa = 11.07 RR186 pKa = 11.84 RR187 pKa = 11.84 HH188 pKa = 6.25 LANVSSQTEE197 pKa = 3.66 ADD199 pKa = 4.06 EE200 pKa = 4.4 ITDD203 pKa = 3.89 EE204 pKa = 4.33 EE205 pKa = 4.43 MLSAAEE211 pKa = 4.11 SMEE214 pKa = 4.4 ADD216 pKa = 3.41 ASNN219 pKa = 3.14
Molecular weight: 25.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.732
IPC2_protein 8.536
IPC_protein 8.521
Toseland 9.107
ProMoST 8.975
Dawson 9.414
Bjellqvist 9.282
Wikipedia 9.575
Rodwell 9.604
Grimsley 9.443
Solomon 9.516
Lehninger 9.487
Nozaki 9.428
DTASelect 9.18
Thurlkill 9.311
EMBOSS 9.575
Sillero 9.458
Patrickios 4.991
IPC_peptide 9.502
IPC2_peptide 8.053
IPC2.peptide.svr19 7.714
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
6
9
4610
198
781
512.2
57.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.705 ± 0.136
2.56 ± 0.306
5.553 ± 0.246
6.009 ± 0.677
3.731 ± 0.123
7.332 ± 0.432
3.189 ± 0.297
4.794 ± 0.244
5.249 ± 0.233
7.896 ± 0.936
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.104 ± 0.152
5.77 ± 0.477
6.79 ± 0.346
5.054 ± 0.119
4.685 ± 0.401
6.421 ± 0.44
7.158 ± 0.433
4.252 ± 0.359
1.887 ± 0.139
3.861 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here