Datura leaf curl virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A289ZLG4|A0A289ZLG4_9GEMI Transcriptional activator protein OS=Datura leaf curl virus OX=1038082 GN=AC2 PE=3 SV=1
MM1 pKa = 7.18GTQSPQFPQSFEE13 pKa = 3.96STQFNMPRR21 pKa = 11.84VNSFQVKK28 pKa = 9.72AKK30 pKa = 10.6NIFLTYY36 pKa = 8.78PKK38 pKa = 10.44CPIPKK43 pKa = 9.32EE44 pKa = 3.95QMLEE48 pKa = 4.14LIKK51 pKa = 10.68SIHH54 pKa = 6.47CPSDD58 pKa = 3.04KK59 pKa = 10.99LFIRR63 pKa = 11.84VAQEE67 pKa = 3.46KK68 pKa = 9.49HH69 pKa = 5.19QDD71 pKa = 3.38GSLHH75 pKa = 5.16IHH77 pKa = 6.91ALIQFKK83 pKa = 10.86GKK85 pKa = 10.05AQFRR89 pKa = 11.84NPRR92 pKa = 11.84HH93 pKa = 5.95FDD95 pKa = 2.89VTHH98 pKa = 6.51PHH100 pKa = 6.51NSSQFHH106 pKa = 7.05PNFQGAKK113 pKa = 9.08SSSDD117 pKa = 3.06VKK119 pKa = 11.23SYY121 pKa = 10.8IEE123 pKa = 4.55KK124 pKa = 10.86DD125 pKa = 2.8GDD127 pKa = 4.02YY128 pKa = 10.58IDD130 pKa = 3.97WGQFQVDD137 pKa = 3.43GRR139 pKa = 11.84SARR142 pKa = 11.84GGQQTANDD150 pKa = 3.91AAAEE154 pKa = 3.97ALNSGSADD162 pKa = 3.14VALAIIRR169 pKa = 11.84EE170 pKa = 4.16KK171 pKa = 10.87LPKK174 pKa = 10.46DD175 pKa = 4.77FIFQYY180 pKa = 11.19HH181 pKa = 5.28NLKK184 pKa = 10.79NNLDD188 pKa = 4.27RR189 pKa = 11.84IFAPPKK195 pKa = 8.91EE196 pKa = 4.67VYY198 pKa = 10.11VSPFLSSSFDD208 pKa = 3.54QVPEE212 pKa = 4.74AIEE215 pKa = 3.95EE216 pKa = 4.43WVSEE220 pKa = 4.06NVMDD224 pKa = 5.26AAARR228 pKa = 11.84PWRR231 pKa = 11.84PNSIVIEE238 pKa = 4.34GDD240 pKa = 3.08SRR242 pKa = 11.84TGKK245 pKa = 8.52TMWARR250 pKa = 11.84SLGPHH255 pKa = 6.71NYY257 pKa = 10.18LCGHH261 pKa = 7.38LDD263 pKa = 4.18LSPKK267 pKa = 10.15VYY269 pKa = 11.07SNDD272 pKa = 2.25AWYY275 pKa = 10.92NVIDD279 pKa = 5.2DD280 pKa = 4.41VDD282 pKa = 3.61PHH284 pKa = 5.85YY285 pKa = 11.0LKK287 pKa = 10.7HH288 pKa = 6.2FKK290 pKa = 10.7EE291 pKa = 4.46FMGAQRR297 pKa = 11.84DD298 pKa = 3.81WQSNTKK304 pKa = 9.26YY305 pKa = 10.63GKK307 pKa = 9.49PIQIKK312 pKa = 10.42GGIPTIFLCNPGPNSSYY329 pKa = 11.34KK330 pKa = 10.62EE331 pKa = 3.85FLDD334 pKa = 4.19EE335 pKa = 5.09EE336 pKa = 4.78KK337 pKa = 11.14NCALKK342 pKa = 10.64AWALKK347 pKa = 10.04NATFISLEE355 pKa = 3.95EE356 pKa = 4.04PLYY359 pKa = 11.15SGTNQGPTQSCC370 pKa = 3.33

Molecular weight: 41.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A289ZNS8|A0A289ZNS8_9GEMI Capsid protein OS=Datura leaf curl virus OX=1038082 GN=AV1 PE=3 SV=1
MM1 pKa = 7.7SKK3 pKa = 10.44RR4 pKa = 11.84PGDD7 pKa = 3.95IIISTPVSKK16 pKa = 10.56VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.07SPYY27 pKa = 10.06SSRR30 pKa = 11.84AAAPIVQGTNRR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84SWTYY47 pKa = 9.26RR48 pKa = 11.84PMYY51 pKa = 9.36RR52 pKa = 11.84KK53 pKa = 9.08PRR55 pKa = 11.84IYY57 pKa = 10.75RR58 pKa = 11.84MYY60 pKa = 10.32RR61 pKa = 11.84SPDD64 pKa = 3.21VPRR67 pKa = 11.84GCEE70 pKa = 4.34GPCKK74 pKa = 9.32VQSYY78 pKa = 7.04EE79 pKa = 3.67QRR81 pKa = 11.84DD82 pKa = 4.2DD83 pKa = 3.51IKK85 pKa = 9.71HH86 pKa = 5.35TGIVRR91 pKa = 11.84CVSDD95 pKa = 3.31VTRR98 pKa = 11.84GSGITHH104 pKa = 6.35RR105 pKa = 11.84VGKK108 pKa = 9.5RR109 pKa = 11.84FCVKK113 pKa = 10.37SIYY116 pKa = 10.32FLGKK120 pKa = 9.15VWMDD124 pKa = 3.41EE125 pKa = 4.15NIKK128 pKa = 10.04KK129 pKa = 9.97QNHH132 pKa = 4.95TNQVMFFLVRR142 pKa = 11.84DD143 pKa = 3.63RR144 pKa = 11.84RR145 pKa = 11.84PYY147 pKa = 10.91GNSPMDD153 pKa = 3.85FGQVFNMFDD162 pKa = 3.99NEE164 pKa = 4.11PSTATVKK171 pKa = 10.85NDD173 pKa = 2.82LRR175 pKa = 11.84DD176 pKa = 3.36RR177 pKa = 11.84FQVMRR182 pKa = 11.84KK183 pKa = 7.65FHH185 pKa = 6.03ATVIGGPSGMKK196 pKa = 9.5EE197 pKa = 3.54QALVKK202 pKa = 10.31RR203 pKa = 11.84FFRR206 pKa = 11.84INSHH210 pKa = 3.89VTYY213 pKa = 10.54NHH215 pKa = 5.79QEE217 pKa = 3.49AAKK220 pKa = 10.4YY221 pKa = 9.25EE222 pKa = 4.13NHH224 pKa = 6.46TEE226 pKa = 3.99NALLLYY232 pKa = 7.29MACTHH237 pKa = 7.07ASNPVYY243 pKa = 10.08ATMKK247 pKa = 9.61IRR249 pKa = 11.84IYY251 pKa = 10.63FYY253 pKa = 11.12DD254 pKa = 3.51SVSNN258 pKa = 3.9

Molecular weight: 30.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1098

85

370

183.0

20.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.373 ± 0.881

2.004 ± 0.417

5.282 ± 0.479

4.372 ± 0.562

4.645 ± 0.763

5.1 ± 0.63

4.281 ± 0.841

6.011 ± 0.401

5.556 ± 0.97

6.557 ± 0.955

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.005 ± 0.806

5.464 ± 0.334

6.375 ± 0.782

5.464 ± 0.488

6.557 ± 1.464

8.106 ± 1.392

5.009 ± 0.912

6.011 ± 0.9

1.366 ± 0.345

3.461 ± 0.558
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski