Amino acid dipepetide frequency for Lactobacillus phage Lc-Nu

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.614AlaAla: 6.614 ± 1.075
0.522AlaCys: 0.522 ± 0.253
7.398AlaAsp: 7.398 ± 1.62
4.787AlaGlu: 4.787 ± 0.92
2.959AlaPhe: 2.959 ± 0.734
5.396AlaGly: 5.396 ± 1.035
1.741AlaHis: 1.741 ± 0.353
5.657AlaIle: 5.657 ± 0.866
6.353AlaLys: 6.353 ± 0.811
7.05AlaLeu: 7.05 ± 0.559
2.698AlaMet: 2.698 ± 0.555
4.961AlaAsn: 4.961 ± 0.824
1.741AlaPro: 1.741 ± 0.471
2.524AlaGln: 2.524 ± 0.845
2.872AlaArg: 2.872 ± 0.428
5.222AlaSer: 5.222 ± 0.604
4.003AlaThr: 4.003 ± 0.458
4.787AlaVal: 4.787 ± 0.833
1.567AlaTrp: 1.567 ± 0.575
3.481AlaTyr: 3.481 ± 0.652
0.0AlaXaa: 0.0 ± 0.0
Cys
0.435CysAla: 0.435 ± 0.214
0.174CysCys: 0.174 ± 0.126
0.261CysAsp: 0.261 ± 0.126
0.348CysGlu: 0.348 ± 0.194
0.0CysPhe: 0.0 ± 0.0
0.174CysGly: 0.174 ± 0.125
0.087CysHis: 0.087 ± 0.086
0.087CysIle: 0.087 ± 0.087
0.261CysLys: 0.261 ± 0.139
0.522CysLeu: 0.522 ± 0.219
0.0CysMet: 0.0 ± 0.0
0.435CysAsn: 0.435 ± 0.192
0.522CysPro: 0.522 ± 0.223
0.348CysGln: 0.348 ± 0.188
0.435CysArg: 0.435 ± 0.205
0.261CysSer: 0.261 ± 0.141
0.261CysThr: 0.261 ± 0.141
0.348CysVal: 0.348 ± 0.176
0.087CysTrp: 0.087 ± 0.087
0.087CysTyr: 0.087 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
5.657AspAla: 5.657 ± 0.934
0.087AspCys: 0.087 ± 0.094
4.439AspAsp: 4.439 ± 0.644
3.394AspGlu: 3.394 ± 0.606
2.611AspPhe: 2.611 ± 0.437
7.05AspGly: 7.05 ± 1.059
1.741AspHis: 1.741 ± 0.474
4.091AspIle: 4.091 ± 0.519
4.961AspLys: 4.961 ± 0.678
6.005AspLeu: 6.005 ± 0.623
1.828AspMet: 1.828 ± 0.479
4.091AspAsn: 4.091 ± 0.716
3.046AspPro: 3.046 ± 0.643
2.524AspGln: 2.524 ± 0.392
4.091AspArg: 4.091 ± 0.567
4.874AspSer: 4.874 ± 0.768
2.698AspThr: 2.698 ± 0.56
4.7AspVal: 4.7 ± 0.703
1.393AspTrp: 1.393 ± 0.301
3.133AspTyr: 3.133 ± 0.681
0.0AspXaa: 0.0 ± 0.0
Glu
3.829GluAla: 3.829 ± 0.671
0.174GluCys: 0.174 ± 0.132
2.698GluAsp: 2.698 ± 0.484
2.698GluGlu: 2.698 ± 0.496
1.567GluPhe: 1.567 ± 0.315
2.35GluGly: 2.35 ± 0.397
1.218GluHis: 1.218 ± 0.401
2.437GluIle: 2.437 ± 0.639
4.091GluLys: 4.091 ± 0.809
4.091GluLeu: 4.091 ± 0.647
1.828GluMet: 1.828 ± 0.319
2.698GluAsn: 2.698 ± 0.549
2.002GluPro: 2.002 ± 0.655
2.089GluGln: 2.089 ± 0.397
2.437GluArg: 2.437 ± 0.674
2.959GluSer: 2.959 ± 0.637
2.872GluThr: 2.872 ± 0.476
2.959GluVal: 2.959 ± 0.447
0.696GluTrp: 0.696 ± 0.192
2.611GluTyr: 2.611 ± 0.563
0.0GluXaa: 0.0 ± 0.0
Phe
2.785PheAla: 2.785 ± 0.492
0.087PheCys: 0.087 ± 0.103
2.35PheAsp: 2.35 ± 0.484
1.741PheGlu: 1.741 ± 0.462
1.393PhePhe: 1.393 ± 0.553
2.959PheGly: 2.959 ± 0.508
0.87PheHis: 0.87 ± 0.329
1.828PheIle: 1.828 ± 0.508
2.611PheLys: 2.611 ± 0.444
3.307PheLeu: 3.307 ± 0.555
0.87PheMet: 0.87 ± 0.282
2.176PheAsn: 2.176 ± 0.41
0.957PhePro: 0.957 ± 0.248
0.783PheGln: 0.783 ± 0.305
0.87PheArg: 0.87 ± 0.376
2.35PheSer: 2.35 ± 0.408
3.394PheThr: 3.394 ± 0.475
2.524PheVal: 2.524 ± 0.444
0.522PheTrp: 0.522 ± 0.18
1.218PheTyr: 1.218 ± 0.346
0.0PheXaa: 0.0 ± 0.0
Gly
4.613GlyAla: 4.613 ± 1.052
0.174GlyCys: 0.174 ± 0.171
5.309GlyAsp: 5.309 ± 0.755
1.393GlyGlu: 1.393 ± 0.295
3.568GlyPhe: 3.568 ± 0.517
4.526GlyGly: 4.526 ± 0.748
0.957GlyHis: 0.957 ± 0.281
5.309GlyIle: 5.309 ± 0.953
7.311GlyLys: 7.311 ± 0.928
4.613GlyLeu: 4.613 ± 1.295
2.089GlyMet: 2.089 ± 0.397
4.091GlyAsn: 4.091 ± 0.786
1.567GlyPro: 1.567 ± 0.613
2.698GlyGln: 2.698 ± 0.42
2.263GlyArg: 2.263 ± 0.523
4.003GlySer: 4.003 ± 0.724
4.613GlyThr: 4.613 ± 0.536
4.526GlyVal: 4.526 ± 0.475
1.218GlyTrp: 1.218 ± 0.346
3.394GlyTyr: 3.394 ± 0.668
0.0GlyXaa: 0.0 ± 0.0
His
1.654HisAla: 1.654 ± 0.386
0.087HisCys: 0.087 ± 0.088
1.654HisAsp: 1.654 ± 0.468
0.609HisGlu: 0.609 ± 0.237
1.131HisPhe: 1.131 ± 0.276
0.783HisGly: 0.783 ± 0.189
0.087HisHis: 0.087 ± 0.092
1.393HisIle: 1.393 ± 0.357
1.305HisLys: 1.305 ± 0.373
0.87HisLeu: 0.87 ± 0.28
0.522HisMet: 0.522 ± 0.26
0.957HisAsn: 0.957 ± 0.404
0.783HisPro: 0.783 ± 0.185
0.696HisGln: 0.696 ± 0.236
0.957HisArg: 0.957 ± 0.346
1.305HisSer: 1.305 ± 0.509
1.305HisThr: 1.305 ± 0.409
1.044HisVal: 1.044 ± 0.211
0.261HisTrp: 0.261 ± 0.138
0.783HisTyr: 0.783 ± 0.262
0.0HisXaa: 0.0 ± 0.0
Ile
6.005IleAla: 6.005 ± 0.614
0.174IleCys: 0.174 ± 0.125
4.7IleAsp: 4.7 ± 0.668
3.829IleGlu: 3.829 ± 0.556
1.131IlePhe: 1.131 ± 0.376
3.829IleGly: 3.829 ± 0.615
1.305IleHis: 1.305 ± 0.314
2.872IleIle: 2.872 ± 0.568
5.135IleLys: 5.135 ± 0.642
3.394IleLeu: 3.394 ± 0.513
1.218IleMet: 1.218 ± 0.368
3.568IleAsn: 3.568 ± 0.49
2.785IlePro: 2.785 ± 0.387
2.785IleGln: 2.785 ± 0.53
3.133IleArg: 3.133 ± 0.653
5.483IleSer: 5.483 ± 0.843
3.829IleThr: 3.829 ± 0.769
4.613IleVal: 4.613 ± 0.684
1.218IleTrp: 1.218 ± 0.473
2.611IleTyr: 2.611 ± 0.439
0.0IleXaa: 0.0 ± 0.0
Lys
7.05LysAla: 7.05 ± 1.154
0.348LysCys: 0.348 ± 0.174
4.352LysAsp: 4.352 ± 0.495
3.046LysGlu: 3.046 ± 0.601
2.872LysPhe: 2.872 ± 0.707
4.178LysGly: 4.178 ± 0.809
1.741LysHis: 1.741 ± 0.32
4.439LysIle: 4.439 ± 0.738
5.396LysLys: 5.396 ± 0.881
5.57LysLeu: 5.57 ± 0.736
1.915LysMet: 1.915 ± 0.459
3.568LysAsn: 3.568 ± 0.462
3.22LysPro: 3.22 ± 0.458
5.135LysGln: 5.135 ± 0.889
3.133LysArg: 3.133 ± 0.54
4.787LysSer: 4.787 ± 0.616
5.831LysThr: 5.831 ± 0.566
4.003LysVal: 4.003 ± 0.677
1.393LysTrp: 1.393 ± 0.345
3.133LysTyr: 3.133 ± 0.642
0.0LysXaa: 0.0 ± 0.0
Leu
8.268LeuAla: 8.268 ± 0.712
0.522LeuCys: 0.522 ± 0.223
4.613LeuAsp: 4.613 ± 0.701
4.526LeuGlu: 4.526 ± 0.889
2.959LeuPhe: 2.959 ± 0.572
5.657LeuGly: 5.657 ± 0.897
1.567LeuHis: 1.567 ± 0.338
5.483LeuIle: 5.483 ± 0.532
4.7LeuLys: 4.7 ± 0.752
4.613LeuLeu: 4.613 ± 0.72
1.741LeuMet: 1.741 ± 0.411
5.135LeuAsn: 5.135 ± 0.764
3.307LeuPro: 3.307 ± 0.537
3.307LeuGln: 3.307 ± 0.592
3.046LeuArg: 3.046 ± 0.548
4.7LeuSer: 4.7 ± 0.582
5.222LeuThr: 5.222 ± 0.744
4.526LeuVal: 4.526 ± 0.678
1.48LeuTrp: 1.48 ± 0.374
2.002LeuTyr: 2.002 ± 0.414
0.0LeuXaa: 0.0 ± 0.0
Met
1.828MetAla: 1.828 ± 0.365
0.174MetCys: 0.174 ± 0.133
1.741MetAsp: 1.741 ± 0.411
0.957MetGlu: 0.957 ± 0.315
0.696MetPhe: 0.696 ± 0.377
1.48MetGly: 1.48 ± 0.381
0.261MetHis: 0.261 ± 0.16
1.393MetIle: 1.393 ± 0.34
1.741MetLys: 1.741 ± 0.35
1.654MetLeu: 1.654 ± 0.406
0.696MetMet: 0.696 ± 0.207
1.654MetAsn: 1.654 ± 0.389
1.044MetPro: 1.044 ± 0.339
1.305MetGln: 1.305 ± 0.283
0.87MetArg: 0.87 ± 0.292
2.176MetSer: 2.176 ± 0.419
1.915MetThr: 1.915 ± 0.428
1.48MetVal: 1.48 ± 0.396
0.087MetTrp: 0.087 ± 0.087
1.567MetTyr: 1.567 ± 0.338
0.0MetXaa: 0.0 ± 0.0
Asn
5.048AsnAla: 5.048 ± 0.828
0.174AsnCys: 0.174 ± 0.113
4.613AsnAsp: 4.613 ± 0.672
3.133AsnGlu: 3.133 ± 0.567
1.48AsnPhe: 1.48 ± 0.305
4.874AsnGly: 4.874 ± 0.696
1.131AsnHis: 1.131 ± 0.247
3.133AsnIle: 3.133 ± 0.573
2.785AsnLys: 2.785 ± 0.499
5.222AsnLeu: 5.222 ± 0.637
1.741AsnMet: 1.741 ± 0.399
2.872AsnAsn: 2.872 ± 0.485
3.046AsnPro: 3.046 ± 0.49
2.611AsnGln: 2.611 ± 0.361
3.046AsnArg: 3.046 ± 0.57
3.133AsnSer: 3.133 ± 0.567
3.568AsnThr: 3.568 ± 0.543
3.046AsnVal: 3.046 ± 0.542
0.87AsnTrp: 0.87 ± 0.281
2.002AsnTyr: 2.002 ± 0.43
0.0AsnXaa: 0.0 ± 0.0
Pro
2.959ProAla: 2.959 ± 0.484
0.087ProCys: 0.087 ± 0.085
2.785ProAsp: 2.785 ± 0.633
2.524ProGlu: 2.524 ± 0.513
1.218ProPhe: 1.218 ± 0.257
2.785ProGly: 2.785 ± 0.802
0.87ProHis: 0.87 ± 0.258
2.35ProIle: 2.35 ± 0.478
3.133ProLys: 3.133 ± 0.463
2.785ProLeu: 2.785 ± 0.504
0.348ProMet: 0.348 ± 0.17
2.263ProAsn: 2.263 ± 0.623
0.87ProPro: 0.87 ± 0.212
1.654ProGln: 1.654 ± 0.455
0.87ProArg: 0.87 ± 0.285
2.785ProSer: 2.785 ± 0.486
1.915ProThr: 1.915 ± 0.397
2.35ProVal: 2.35 ± 0.556
0.522ProTrp: 0.522 ± 0.262
1.393ProTyr: 1.393 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
4.439GlnAla: 4.439 ± 0.752
0.0GlnCys: 0.0 ± 0.0
2.872GlnAsp: 2.872 ± 0.486
1.915GlnGlu: 1.915 ± 0.463
1.654GlnPhe: 1.654 ± 0.389
2.524GlnGly: 2.524 ± 0.56
0.696GlnHis: 0.696 ± 0.22
3.481GlnIle: 3.481 ± 0.549
2.437GlnLys: 2.437 ± 0.427
4.613GlnLeu: 4.613 ± 0.766
1.305GlnMet: 1.305 ± 0.37
2.35GlnAsn: 2.35 ± 0.488
1.741GlnPro: 1.741 ± 0.399
3.046GlnGln: 3.046 ± 0.778
1.654GlnArg: 1.654 ± 0.302
2.263GlnSer: 2.263 ± 0.389
1.915GlnThr: 1.915 ± 0.352
2.785GlnVal: 2.785 ± 0.609
0.87GlnTrp: 0.87 ± 0.333
2.176GlnTyr: 2.176 ± 0.353
0.0GlnXaa: 0.0 ± 0.0
Arg
2.437ArgAla: 2.437 ± 0.406
0.348ArgCys: 0.348 ± 0.21
3.307ArgAsp: 3.307 ± 0.771
2.437ArgGlu: 2.437 ± 0.504
1.915ArgPhe: 1.915 ± 0.388
2.698ArgGly: 2.698 ± 0.707
0.522ArgHis: 0.522 ± 0.214
2.35ArgIle: 2.35 ± 0.536
3.307ArgLys: 3.307 ± 0.71
4.091ArgLeu: 4.091 ± 0.864
0.261ArgMet: 0.261 ± 0.149
1.48ArgAsn: 1.48 ± 0.334
1.044ArgPro: 1.044 ± 0.289
2.176ArgGln: 2.176 ± 0.514
1.741ArgArg: 1.741 ± 0.479
2.263ArgSer: 2.263 ± 0.372
3.046ArgThr: 3.046 ± 0.502
3.22ArgVal: 3.22 ± 0.589
0.957ArgTrp: 0.957 ± 0.266
1.828ArgTyr: 1.828 ± 0.501
0.0ArgXaa: 0.0 ± 0.0
Ser
4.961SerAla: 4.961 ± 1.008
0.261SerCys: 0.261 ± 0.145
5.57SerAsp: 5.57 ± 0.921
3.307SerGlu: 3.307 ± 0.481
2.524SerPhe: 2.524 ± 0.56
4.874SerGly: 4.874 ± 0.723
1.131SerHis: 1.131 ± 0.334
5.048SerIle: 5.048 ± 0.505
4.961SerLys: 4.961 ± 0.723
4.874SerLeu: 4.874 ± 0.584
1.393SerMet: 1.393 ± 0.356
4.787SerAsn: 4.787 ± 0.677
2.002SerPro: 2.002 ± 0.539
3.307SerGln: 3.307 ± 0.61
3.22SerArg: 3.22 ± 0.425
4.265SerSer: 4.265 ± 1.063
4.352SerThr: 4.352 ± 0.7
3.568SerVal: 3.568 ± 0.555
1.305SerTrp: 1.305 ± 0.387
2.176SerTyr: 2.176 ± 0.648
0.0SerXaa: 0.0 ± 0.0
Thr
5.222ThrAla: 5.222 ± 0.654
0.435ThrCys: 0.435 ± 0.175
5.396ThrAsp: 5.396 ± 0.685
2.437ThrGlu: 2.437 ± 0.488
1.567ThrPhe: 1.567 ± 0.306
3.742ThrGly: 3.742 ± 0.574
0.435ThrHis: 0.435 ± 0.166
4.874ThrIle: 4.874 ± 0.91
4.265ThrLys: 4.265 ± 0.542
5.483ThrLeu: 5.483 ± 0.744
1.393ThrMet: 1.393 ± 0.487
3.916ThrAsn: 3.916 ± 0.628
1.828ThrPro: 1.828 ± 0.454
2.002ThrGln: 2.002 ± 0.362
2.35ThrArg: 2.35 ± 0.34
4.787ThrSer: 4.787 ± 0.742
4.352ThrThr: 4.352 ± 0.779
4.265ThrVal: 4.265 ± 0.674
0.696ThrTrp: 0.696 ± 0.265
2.872ThrTyr: 2.872 ± 0.834
0.0ThrXaa: 0.0 ± 0.0
Val
4.7ValAla: 4.7 ± 0.594
0.696ValCys: 0.696 ± 0.211
4.613ValAsp: 4.613 ± 0.642
2.698ValGlu: 2.698 ± 0.696
1.828ValPhe: 1.828 ± 0.378
4.874ValGly: 4.874 ± 0.721
0.783ValHis: 0.783 ± 0.245
3.394ValIle: 3.394 ± 0.518
5.831ValLys: 5.831 ± 0.991
4.439ValLeu: 4.439 ± 0.652
1.567ValMet: 1.567 ± 0.469
2.959ValAsn: 2.959 ± 0.395
2.524ValPro: 2.524 ± 0.655
2.611ValGln: 2.611 ± 0.495
1.915ValArg: 1.915 ± 0.411
4.874ValSer: 4.874 ± 0.579
3.568ValThr: 3.568 ± 0.585
3.133ValVal: 3.133 ± 0.537
1.218ValTrp: 1.218 ± 0.589
2.176ValTyr: 2.176 ± 0.423
0.0ValXaa: 0.0 ± 0.0
Trp
1.218TrpAla: 1.218 ± 0.393
0.174TrpCys: 0.174 ± 0.127
0.696TrpAsp: 0.696 ± 0.414
0.783TrpGlu: 0.783 ± 0.218
0.522TrpPhe: 0.522 ± 0.195
0.87TrpGly: 0.87 ± 0.452
0.261TrpHis: 0.261 ± 0.144
1.393TrpIle: 1.393 ± 0.368
1.654TrpLys: 1.654 ± 0.476
1.393TrpLeu: 1.393 ± 0.275
0.261TrpMet: 0.261 ± 0.21
1.654TrpAsn: 1.654 ± 0.697
0.435TrpPro: 0.435 ± 0.274
1.131TrpGln: 1.131 ± 0.224
0.957TrpArg: 0.957 ± 0.316
1.131TrpSer: 1.131 ± 0.289
0.957TrpThr: 0.957 ± 0.265
0.87TrpVal: 0.87 ± 0.194
0.522TrpTrp: 0.522 ± 0.19
0.435TrpTyr: 0.435 ± 0.184
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.611TyrAla: 2.611 ± 0.619
0.435TyrCys: 0.435 ± 0.168
3.046TyrAsp: 3.046 ± 0.533
1.741TyrGlu: 1.741 ± 0.512
2.002TyrPhe: 2.002 ± 0.523
2.611TyrGly: 2.611 ± 0.583
0.783TyrHis: 0.783 ± 0.265
2.611TyrIle: 2.611 ± 0.712
2.872TyrLys: 2.872 ± 0.654
2.872TyrLeu: 2.872 ± 0.655
0.957TyrMet: 0.957 ± 0.249
1.915TyrAsn: 1.915 ± 0.402
2.002TyrPro: 2.002 ± 0.338
2.002TyrGln: 2.002 ± 0.443
1.567TyrArg: 1.567 ± 0.501
4.265TyrSer: 4.265 ± 0.965
2.698TyrThr: 2.698 ± 0.725
1.654TyrVal: 1.654 ± 0.358
0.435TyrTrp: 0.435 ± 0.233
1.828TyrTyr: 1.828 ± 0.454
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (11491 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski