Amino acid dipepetide frequency for Chromobacterium sp. LK11

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.984AlaAla: 18.984 ± 0.23
1.435AlaCys: 1.435 ± 0.032
7.034AlaAsp: 7.034 ± 0.091
7.762AlaGlu: 7.762 ± 0.101
3.911AlaPhe: 3.911 ± 0.062
11.3AlaGly: 11.3 ± 0.103
2.154AlaHis: 2.154 ± 0.039
4.958AlaIle: 4.958 ± 0.064
4.098AlaLys: 4.098 ± 0.073
15.873AlaLeu: 15.873 ± 0.153
3.39AlaMet: 3.39 ± 0.057
2.967AlaAsn: 2.967 ± 0.051
5.719AlaPro: 5.719 ± 0.093
5.287AlaGln: 5.287 ± 0.077
8.604AlaArg: 8.604 ± 0.101
6.398AlaSer: 6.398 ± 0.078
4.423AlaThr: 4.423 ± 0.058
8.085AlaVal: 8.085 ± 0.077
1.921AlaTrp: 1.921 ± 0.044
2.671AlaTyr: 2.671 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
1.255CysAla: 1.255 ± 0.031
0.158CysCys: 0.158 ± 0.012
0.53CysAsp: 0.53 ± 0.021
0.53CysGlu: 0.53 ± 0.018
0.34CysPhe: 0.34 ± 0.015
1.079CysGly: 1.079 ± 0.03
0.289CysHis: 0.289 ± 0.014
0.334CysIle: 0.334 ± 0.016
0.267CysLys: 0.267 ± 0.015
1.155CysLeu: 1.155 ± 0.027
0.185CysMet: 0.185 ± 0.011
0.242CysAsn: 0.242 ± 0.013
0.533CysPro: 0.533 ± 0.019
0.417CysGln: 0.417 ± 0.02
0.798CysArg: 0.798 ± 0.03
0.511CysSer: 0.511 ± 0.021
0.32CysThr: 0.32 ± 0.016
0.703CysVal: 0.703 ± 0.021
0.161CysTrp: 0.161 ± 0.012
0.278CysTyr: 0.278 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
7.058AspAla: 7.058 ± 0.096
0.579AspCys: 0.579 ± 0.019
2.779AspAsp: 2.779 ± 0.052
3.158AspGlu: 3.158 ± 0.05
2.167AspPhe: 2.167 ± 0.04
5.125AspGly: 5.125 ± 0.077
1.016AspHis: 1.016 ± 0.029
2.875AspIle: 2.875 ± 0.051
2.155AspLys: 2.155 ± 0.042
5.115AspLeu: 5.115 ± 0.067
1.269AspMet: 1.269 ± 0.031
1.464AspAsn: 1.464 ± 0.033
2.678AspPro: 2.678 ± 0.044
1.768AspGln: 1.768 ± 0.038
2.784AspArg: 2.784 ± 0.049
2.802AspSer: 2.802 ± 0.054
2.076AspThr: 2.076 ± 0.047
3.346AspVal: 3.346 ± 0.057
1.135AspTrp: 1.135 ± 0.035
1.812AspTyr: 1.812 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
7.102GluAla: 7.102 ± 0.091
0.436GluCys: 0.436 ± 0.018
2.406GluAsp: 2.406 ± 0.04
2.892GluGlu: 2.892 ± 0.05
1.687GluPhe: 1.687 ± 0.035
3.235GluGly: 3.235 ± 0.05
1.391GluHis: 1.391 ± 0.035
2.711GluIle: 2.711 ± 0.045
1.823GluLys: 1.823 ± 0.044
6.991GluLeu: 6.991 ± 0.072
1.38GluMet: 1.38 ± 0.033
1.343GluAsn: 1.343 ± 0.036
2.441GluPro: 2.441 ± 0.049
3.454GluGln: 3.454 ± 0.061
5.001GluArg: 5.001 ± 0.085
2.442GluSer: 2.442 ± 0.046
2.453GluThr: 2.453 ± 0.042
3.869GluVal: 3.869 ± 0.064
0.784GluTrp: 0.784 ± 0.024
1.16GluTyr: 1.16 ± 0.03
0.0GluXaa: 0.0 ± 0.0
Phe
4.197PheAla: 4.197 ± 0.06
0.42PheCys: 0.42 ± 0.017
2.425PheAsp: 2.425 ± 0.047
1.812PheGlu: 1.812 ± 0.038
1.39PhePhe: 1.39 ± 0.036
3.236PheGly: 3.236 ± 0.052
0.811PheHis: 0.811 ± 0.024
1.672PheIle: 1.672 ± 0.039
1.205PheLys: 1.205 ± 0.033
3.206PheLeu: 3.206 ± 0.058
0.793PheMet: 0.793 ± 0.027
1.307PheAsn: 1.307 ± 0.037
1.456PhePro: 1.456 ± 0.035
1.232PheGln: 1.232 ± 0.034
1.942PheArg: 1.942 ± 0.043
2.39PheSer: 2.39 ± 0.043
1.612PheThr: 1.612 ± 0.037
2.398PheVal: 2.398 ± 0.044
0.563PheTrp: 0.563 ± 0.025
0.995PheTyr: 0.995 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
8.756GlyAla: 8.756 ± 0.085
0.989GlyCys: 0.989 ± 0.026
4.169GlyAsp: 4.169 ± 0.06
4.81GlyGlu: 4.81 ± 0.072
3.411GlyPhe: 3.411 ± 0.056
7.209GlyGly: 7.209 ± 0.107
1.835GlyHis: 1.835 ± 0.04
3.579GlyIle: 3.579 ± 0.063
3.729GlyLys: 3.729 ± 0.066
9.508GlyLeu: 9.508 ± 0.098
2.325GlyMet: 2.325 ± 0.043
2.271GlyAsn: 2.271 ± 0.055
2.649GlyPro: 2.649 ± 0.048
3.605GlyGln: 3.605 ± 0.056
5.569GlyArg: 5.569 ± 0.077
4.423GlySer: 4.423 ± 0.071
2.824GlyThr: 2.824 ± 0.057
6.552GlyVal: 6.552 ± 0.075
1.569GlyTrp: 1.569 ± 0.041
2.486GlyTyr: 2.486 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.426HisAla: 2.426 ± 0.042
0.312HisCys: 0.312 ± 0.016
1.081HisAsp: 1.081 ± 0.028
0.984HisGlu: 0.984 ± 0.028
0.919HisPhe: 0.919 ± 0.027
2.082HisGly: 2.082 ± 0.042
0.583HisHis: 0.583 ± 0.023
0.955HisIle: 0.955 ± 0.026
0.569HisLys: 0.569 ± 0.02
2.192HisLeu: 2.192 ± 0.04
0.436HisMet: 0.436 ± 0.017
0.558HisAsn: 0.558 ± 0.019
1.528HisPro: 1.528 ± 0.037
0.908HisGln: 0.908 ± 0.028
1.319HisArg: 1.319 ± 0.036
1.148HisSer: 1.148 ± 0.031
0.787HisThr: 0.787 ± 0.028
1.32HisVal: 1.32 ± 0.036
0.412HisTrp: 0.412 ± 0.017
0.714HisTyr: 0.714 ± 0.024
0.0HisXaa: 0.0 ± 0.0
Ile
5.808IleAla: 5.808 ± 0.073
0.439IleCys: 0.439 ± 0.017
2.744IleAsp: 2.744 ± 0.05
2.637IleGlu: 2.637 ± 0.048
1.217IlePhe: 1.217 ± 0.031
4.15IleGly: 4.15 ± 0.063
0.906IleHis: 0.906 ± 0.026
1.693IleIle: 1.693 ± 0.043
1.676IleLys: 1.676 ± 0.046
3.72IleLeu: 3.72 ± 0.06
0.825IleMet: 0.825 ± 0.026
1.468IleAsn: 1.468 ± 0.038
1.963IlePro: 1.963 ± 0.043
1.481IleGln: 1.481 ± 0.032
2.816IleArg: 2.816 ± 0.048
2.565IleSer: 2.565 ± 0.053
1.981IleThr: 1.981 ± 0.039
3.285IleVal: 3.285 ± 0.051
0.468IleTrp: 0.468 ± 0.02
0.996IleTyr: 0.996 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.874LysAla: 3.874 ± 0.063
0.159LysCys: 0.159 ± 0.013
1.723LysAsp: 1.723 ± 0.041
1.639LysGlu: 1.639 ± 0.041
0.834LysPhe: 0.834 ± 0.026
2.438LysGly: 2.438 ± 0.062
0.69LysHis: 0.69 ± 0.023
1.519LysIle: 1.519 ± 0.039
1.378LysLys: 1.378 ± 0.045
4.448LysLeu: 4.448 ± 0.064
0.892LysMet: 0.892 ± 0.029
1.046LysAsn: 1.046 ± 0.035
2.251LysPro: 2.251 ± 0.046
1.703LysGln: 1.703 ± 0.036
2.27LysArg: 2.27 ± 0.048
1.833LysSer: 1.833 ± 0.041
1.888LysThr: 1.888 ± 0.043
2.599LysVal: 2.599 ± 0.053
0.347LysTrp: 0.347 ± 0.016
0.671LysTyr: 0.671 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
16.695LeuAla: 16.695 ± 0.17
1.165LeuCys: 1.165 ± 0.033
6.673LeuAsp: 6.673 ± 0.077
6.274LeuGlu: 6.274 ± 0.078
3.848LeuPhe: 3.848 ± 0.065
8.928LeuGly: 8.928 ± 0.091
2.134LeuHis: 2.134 ± 0.039
4.977LeuIle: 4.977 ± 0.067
4.211LeuLys: 4.211 ± 0.066
13.868LeuLeu: 13.868 ± 0.151
2.646LeuMet: 2.646 ± 0.044
3.472LeuAsn: 3.472 ± 0.053
6.979LeuPro: 6.979 ± 0.091
4.581LeuGln: 4.581 ± 0.066
8.034LeuArg: 8.034 ± 0.088
8.32LeuSer: 8.32 ± 0.084
5.368LeuThr: 5.368 ± 0.068
6.693LeuVal: 6.693 ± 0.081
1.452LeuTrp: 1.452 ± 0.039
2.416LeuTyr: 2.416 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.918MetAla: 2.918 ± 0.052
0.175MetCys: 0.175 ± 0.011
1.118MetAsp: 1.118 ± 0.028
1.175MetGlu: 1.175 ± 0.03
0.677MetPhe: 0.677 ± 0.024
1.664MetGly: 1.664 ± 0.039
0.454MetHis: 0.454 ± 0.019
0.989MetIle: 0.989 ± 0.026
1.001MetLys: 1.001 ± 0.03
3.005MetLeu: 3.005 ± 0.05
0.641MetMet: 0.641 ± 0.025
0.772MetAsn: 0.772 ± 0.026
1.329MetPro: 1.329 ± 0.034
1.073MetGln: 1.073 ± 0.03
1.669MetArg: 1.669 ± 0.03
1.719MetSer: 1.719 ± 0.032
1.28MetThr: 1.28 ± 0.033
1.582MetVal: 1.582 ± 0.034
0.192MetTrp: 0.192 ± 0.013
0.379MetTyr: 0.379 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.162AsnAla: 3.162 ± 0.054
0.271AsnCys: 0.271 ± 0.012
1.378AsnAsp: 1.378 ± 0.043
1.224AsnGlu: 1.224 ± 0.029
0.932AsnPhe: 0.932 ± 0.031
2.476AsnGly: 2.476 ± 0.049
0.627AsnHis: 0.627 ± 0.025
1.388AsnIle: 1.388 ± 0.037
0.905AsnLys: 0.905 ± 0.027
2.967AsnLeu: 2.967 ± 0.045
0.63AsnMet: 0.63 ± 0.021
0.854AsnAsn: 0.854 ± 0.033
1.882AsnPro: 1.882 ± 0.04
1.239AsnGln: 1.239 ± 0.036
1.807AsnArg: 1.807 ± 0.03
1.394AsnSer: 1.394 ± 0.042
1.327AsnThr: 1.327 ± 0.036
1.824AsnVal: 1.824 ± 0.042
0.455AsnTrp: 0.455 ± 0.019
0.846AsnTyr: 0.846 ± 0.026
0.0AsnXaa: 0.0 ± 0.0
Pro
7.328ProAla: 7.328 ± 0.103
0.405ProCys: 0.405 ± 0.018
3.126ProAsp: 3.126 ± 0.051
3.55ProGlu: 3.55 ± 0.053
1.79ProPhe: 1.79 ± 0.035
4.125ProGly: 4.125 ± 0.056
1.013ProHis: 1.013 ± 0.025
1.694ProIle: 1.694 ± 0.039
1.479ProLys: 1.479 ± 0.038
5.962ProLeu: 5.962 ± 0.079
1.019ProMet: 1.019 ± 0.024
1.238ProAsn: 1.238 ± 0.032
2.622ProPro: 2.622 ± 0.051
2.347ProGln: 2.347 ± 0.046
2.875ProArg: 2.875 ± 0.055
2.546ProSer: 2.546 ± 0.04
1.853ProThr: 1.853 ± 0.04
3.512ProVal: 3.512 ± 0.054
0.79ProTrp: 0.79 ± 0.022
1.271ProTyr: 1.271 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
6.38GlnAla: 6.38 ± 0.079
0.331GlnCys: 0.331 ± 0.017
1.939GlnAsp: 1.939 ± 0.039
1.792GlnGlu: 1.792 ± 0.039
1.276GlnPhe: 1.276 ± 0.027
3.424GlnGly: 3.424 ± 0.057
1.013GlnHis: 1.013 ± 0.026
1.759GlnIle: 1.759 ± 0.041
1.191GlnLys: 1.191 ± 0.031
5.611GlnLeu: 5.611 ± 0.079
1.0GlnMet: 1.0 ± 0.025
1.024GlnAsn: 1.024 ± 0.032
2.527GlnPro: 2.527 ± 0.05
2.742GlnGln: 2.742 ± 0.048
3.539GlnArg: 3.539 ± 0.059
2.252GlnSer: 2.252 ± 0.041
1.988GlnThr: 1.988 ± 0.046
2.926GlnVal: 2.926 ± 0.05
0.743GlnTrp: 0.743 ± 0.025
0.959GlnTyr: 0.959 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
7.283ArgAla: 7.283 ± 0.101
0.632ArgCys: 0.632 ± 0.024
3.747ArgAsp: 3.747 ± 0.055
4.34ArgGlu: 4.34 ± 0.065
2.935ArgPhe: 2.935 ± 0.05
4.574ArgGly: 4.574 ± 0.065
1.952ArgHis: 1.952 ± 0.035
3.118ArgIle: 3.118 ± 0.051
2.098ArgLys: 2.098 ± 0.045
9.056ArgLeu: 9.056 ± 0.109
1.637ArgMet: 1.637 ± 0.04
1.707ArgAsn: 1.707 ± 0.034
3.293ArgPro: 3.293 ± 0.047
4.1ArgGln: 4.1 ± 0.076
5.48ArgArg: 5.48 ± 0.078
3.063ArgSer: 3.063 ± 0.051
2.146ArgThr: 2.146 ± 0.043
4.602ArgVal: 4.602 ± 0.061
1.097ArgTrp: 1.097 ± 0.03
2.14ArgTyr: 2.14 ± 0.045
0.0ArgXaa: 0.0 ± 0.0
Ser
6.739SerAla: 6.739 ± 0.083
0.642SerCys: 0.642 ± 0.023
2.607SerAsp: 2.607 ± 0.046
2.702SerGlu: 2.702 ± 0.038
2.19SerPhe: 2.19 ± 0.044
5.492SerGly: 5.492 ± 0.074
1.307SerHis: 1.307 ± 0.03
2.344SerIle: 2.344 ± 0.045
1.739SerLys: 1.739 ± 0.041
6.634SerLeu: 6.634 ± 0.082
1.307SerMet: 1.307 ± 0.035
1.563SerAsn: 1.563 ± 0.044
2.744SerPro: 2.744 ± 0.049
2.375SerGln: 2.375 ± 0.045
3.879SerArg: 3.879 ± 0.057
3.188SerSer: 3.188 ± 0.077
2.233SerThr: 2.233 ± 0.052
3.749SerVal: 3.749 ± 0.059
0.863SerTrp: 0.863 ± 0.027
1.577SerTyr: 1.577 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
4.632ThrAla: 4.632 ± 0.069
0.331ThrCys: 0.331 ± 0.014
1.845ThrAsp: 1.845 ± 0.043
1.963ThrGlu: 1.963 ± 0.041
1.38ThrPhe: 1.38 ± 0.035
3.593ThrGly: 3.593 ± 0.08
0.869ThrHis: 0.869 ± 0.027
1.712ThrIle: 1.712 ± 0.041
0.919ThrLys: 0.919 ± 0.027
6.094ThrLeu: 6.094 ± 0.071
0.917ThrMet: 0.917 ± 0.025
0.986ThrAsn: 0.986 ± 0.032
2.862ThrPro: 2.862 ± 0.05
1.588ThrGln: 1.588 ± 0.039
2.756ThrArg: 2.756 ± 0.045
2.134ThrSer: 2.134 ± 0.056
1.819ThrThr: 1.819 ± 0.042
3.494ThrVal: 3.494 ± 0.06
0.45ThrTrp: 0.45 ± 0.019
0.838ThrTyr: 0.838 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
8.372ValAla: 8.372 ± 0.078
0.763ValCys: 0.763 ± 0.027
3.919ValAsp: 3.919 ± 0.062
4.104ValGlu: 4.104 ± 0.057
2.606ValPhe: 2.606 ± 0.048
5.12ValGly: 5.12 ± 0.063
1.238ValHis: 1.238 ± 0.032
3.184ValIle: 3.184 ± 0.056
2.622ValLys: 2.622 ± 0.051
7.628ValLeu: 7.628 ± 0.087
1.73ValMet: 1.73 ± 0.036
2.201ValAsn: 2.201 ± 0.044
2.983ValPro: 2.983 ± 0.051
2.148ValGln: 2.148 ± 0.038
4.216ValArg: 4.216 ± 0.062
4.477ValSer: 4.477 ± 0.057
3.136ValThr: 3.136 ± 0.065
5.167ValVal: 5.167 ± 0.073
0.913ValTrp: 0.913 ± 0.03
1.618ValTyr: 1.618 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.228TrpAla: 1.228 ± 0.031
0.152TrpCys: 0.152 ± 0.011
0.631TrpAsp: 0.631 ± 0.024
0.598TrpGlu: 0.598 ± 0.024
0.546TrpPhe: 0.546 ± 0.019
0.957TrpGly: 0.957 ± 0.031
0.391TrpHis: 0.391 ± 0.018
0.541TrpIle: 0.541 ± 0.022
0.457TrpLys: 0.457 ± 0.021
2.592TrpLeu: 2.592 ± 0.054
0.361TrpMet: 0.361 ± 0.017
0.412TrpAsn: 0.412 ± 0.018
0.769TrpPro: 0.769 ± 0.025
0.997TrpGln: 0.997 ± 0.031
1.521TrpArg: 1.521 ± 0.04
0.884TrpSer: 0.884 ± 0.027
0.572TrpThr: 0.572 ± 0.019
0.884TrpVal: 0.884 ± 0.028
0.266TrpTrp: 0.266 ± 0.016
0.295TrpTyr: 0.295 ± 0.016
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.776TyrAla: 2.776 ± 0.044
0.292TyrCys: 0.292 ± 0.015
1.284TyrAsp: 1.284 ± 0.028
1.055TyrGlu: 1.055 ± 0.029
1.002TyrPhe: 1.002 ± 0.025
2.063TyrGly: 2.063 ± 0.042
0.557TyrHis: 0.557 ± 0.021
0.837TyrIle: 0.837 ± 0.028
0.679TyrLys: 0.679 ± 0.023
3.107TyrLeu: 3.107 ± 0.057
0.431TyrMet: 0.431 ± 0.017
0.695TyrAsn: 0.695 ± 0.027
1.337TyrPro: 1.337 ± 0.035
1.375TyrGln: 1.375 ± 0.033
2.122TyrArg: 2.122 ± 0.048
1.382TyrSer: 1.382 ± 0.036
1.095TyrThr: 1.095 ± 0.029
1.557TyrVal: 1.557 ± 0.036
0.476TyrTrp: 0.476 ± 0.018
0.708TyrTyr: 0.708 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4215 proteins (1370590 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski