Rhodovulum phage vB_RhkS_P1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1P722|A0A1B1P722_9CAUD Uncharacterized protein OS=Rhodovulum phage vB_RhkS_P1 OX=1873452 GN=Rhks_45 PE=4 SV=1
MM1 pKa = 7.13SVCLEE6 pKa = 3.87SMYY9 pKa = 10.84TGGQLAAALSCDD21 pKa = 3.86PEE23 pKa = 4.08EE24 pKa = 3.85FAYY27 pKa = 10.66FLVGLSEE34 pKa = 4.68DD35 pKa = 3.49VGPTFVANVAEE46 pKa = 4.36YY47 pKa = 10.63FPFGADD53 pKa = 3.1AEE55 pKa = 4.86GVARR59 pKa = 11.84LLRR62 pKa = 11.84ALADD66 pKa = 4.26EE67 pKa = 4.64IEE69 pKa = 4.15RR70 pKa = 4.21

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1P6Z9|A0A1B1P6Z9_9CAUD Uncharacterized protein OS=Rhodovulum phage vB_RhkS_P1 OX=1873452 GN=Rhks_14 PE=4 SV=1
MM1 pKa = 7.18SRR3 pKa = 11.84KK4 pKa = 9.32PVDD7 pKa = 3.48QLATSSKK14 pKa = 10.22PQGQDD19 pKa = 3.41GVWACLRR26 pKa = 11.84MLRR29 pKa = 11.84TATVGEE35 pKa = 4.33IVRR38 pKa = 11.84EE39 pKa = 3.59TDD41 pKa = 2.88INRR44 pKa = 11.84KK45 pKa = 6.51TVADD49 pKa = 3.71YY50 pKa = 10.7LHH52 pKa = 7.08RR53 pKa = 11.84LLAGGYY59 pKa = 6.95VTVEE63 pKa = 4.03GTPARR68 pKa = 11.84YY69 pKa = 7.79TLIRR73 pKa = 11.84DD74 pKa = 4.12AGHH77 pKa = 6.15HH78 pKa = 5.93APRR81 pKa = 11.84LRR83 pKa = 11.84ADD85 pKa = 3.99GSAVTQGAGTRR96 pKa = 11.84NMWRR100 pKa = 11.84SMNMMGEE107 pKa = 4.41FSALDD112 pKa = 3.72LAVHH116 pKa = 5.74STAGDD121 pKa = 3.64VTVTEE126 pKa = 4.6ATAKK130 pKa = 10.23SYY132 pKa = 9.14CTALLKK138 pKa = 10.63AGYY141 pKa = 9.99LRR143 pKa = 11.84VVQKK147 pKa = 10.57ARR149 pKa = 11.84PGVRR153 pKa = 11.84PAIYY157 pKa = 10.05RR158 pKa = 11.84LVRR161 pKa = 11.84KK162 pKa = 7.44TGPRR166 pKa = 11.84APQIQRR172 pKa = 11.84VKK174 pKa = 10.65QVWDD178 pKa = 3.88PNLGAVMSPKK188 pKa = 10.0TEE190 pKa = 3.78ARR192 pKa = 11.84SS193 pKa = 3.35

Molecular weight: 21.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

12173

40

1845

206.3

22.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.393 ± 0.469

0.813 ± 0.104

7.098 ± 0.278

6.005 ± 0.286

2.859 ± 0.17

8.264 ± 0.254

1.931 ± 0.211

4.518 ± 0.205

2.497 ± 0.251

8.757 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.169

1.939 ± 0.203

5.373 ± 0.322

3.27 ± 0.206

8.092 ± 0.542

4.855 ± 0.41

5.98 ± 0.394

6.892 ± 0.404

1.955 ± 0.163

2.119 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski