Beihai weivirus-like virus 2
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KL03|A0A1L3KL03_9VIRU RNA-dependent RNA polymerase OS=Beihai weivirus-like virus 2 OX=1922748 PE=4 SV=1
MM1 pKa = 7.31 LQGDD5 pKa = 4.29 VANTPALALVGTQPPPGLSTCSVRR29 pKa = 11.84 QTGVLGGVHH38 pKa = 6.63 ARR40 pKa = 11.84 TAGRR44 pKa = 11.84 NIPGPVPGSPAWLVWVHH61 pKa = 6.38 DD62 pKa = 4.7 HH63 pKa = 7.18 FDD65 pKa = 3.71 HH66 pKa = 7.85 PDD68 pKa = 3.5 GEE70 pKa = 5.1 GVRR73 pKa = 11.84 EE74 pKa = 3.76 THH76 pKa = 6.29 GRR78 pKa = 11.84 GEE80 pKa = 4.15 AARR83 pKa = 11.84 HH84 pKa = 5.45 GEE86 pKa = 4.34 GEE88 pKa = 4.34 GSSVAGSSQHH98 pKa = 6.38 RR99 pKa = 11.84 GQRR102 pKa = 11.84 TLEE105 pKa = 3.85 DD106 pKa = 3.17 RR107 pKa = 11.84 EE108 pKa = 4.03 YY109 pKa = 9.37 RR110 pKa = 11.84 TCGTQVFGTGHH121 pKa = 7.09 DD122 pKa = 3.63 NPRR125 pKa = 11.84 GQGVEE130 pKa = 4.12 HH131 pKa = 6.81 NGHH134 pKa = 6.22 HH135 pKa = 6.42 RR136 pKa = 11.84 RR137 pKa = 11.84 PSFIAYY143 pKa = 9.72 DD144 pKa = 3.54 IVVHH148 pKa = 6.88 DD149 pKa = 4.9 FGIPPARR156 pKa = 11.84 VGSLADD162 pKa = 3.3 GHH164 pKa = 5.62 VAPTVGEE171 pKa = 4.07 QTILEE176 pKa = 4.39 AASDD180 pKa = 3.76 AGDD183 pKa = 3.33 VDD185 pKa = 3.95 GEE187 pKa = 4.39 RR188 pKa = 11.84 VQGGARR194 pKa = 11.84 EE195 pKa = 3.99 AAVGGGQPWEE205 pKa = 4.1 AGLRR209 pKa = 11.84 LARR212 pKa = 11.84 GRR214 pKa = 11.84 LGGQTDD220 pKa = 3.93 DD221 pKa = 4.35 EE222 pKa = 6.1 DD223 pKa = 5.43 DD224 pKa = 4.27 GFVGARR230 pKa = 11.84 WMGCMVWSSGVHH242 pKa = 4.95 GHH244 pKa = 7.34 DD245 pKa = 4.66 EE246 pKa = 4.52 VPCDD250 pKa = 3.83 DD251 pKa = 5.1 LLSEE255 pKa = 4.26 THH257 pKa = 6.56 ASARR261 pKa = 11.84 LLEE264 pKa = 4.44 RR265 pKa = 11.84 TDD267 pKa = 3.51 SQFHH271 pKa = 6.22 FGWW274 pKa = 4.47
Molecular weight: 28.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.4
IPC2_protein 5.232
IPC_protein 5.245
Toseland 5.614
ProMoST 5.55
Dawson 5.448
Bjellqvist 5.486
Wikipedia 5.423
Rodwell 5.41
Grimsley 5.766
Solomon 5.448
Lehninger 5.423
Nozaki 5.677
DTASelect 5.868
Thurlkill 5.804
EMBOSS 5.766
Sillero 5.766
Patrickios 4.113
IPC_peptide 5.461
IPC2_peptide 5.792
IPC2.peptide.svr19 5.698
Protein with the highest isoelectric point:
>tr|A0A1L3KL47|A0A1L3KL47_9VIRU Putative capsid protein OS=Beihai weivirus-like virus 2 OX=1922748 PE=4 SV=1
MM1 pKa = 7.55 AKK3 pKa = 10.18 KK4 pKa = 9.76 FVKK7 pKa = 10.26 KK8 pKa = 10.4 LPQRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 PVIKK19 pKa = 10.17 RR20 pKa = 11.84 NMKK23 pKa = 8.84 ATRR26 pKa = 11.84 SHH28 pKa = 6.05 ATRR31 pKa = 11.84 VLAQGAGAITKK42 pKa = 9.97 KK43 pKa = 10.85 AFGAGKK49 pKa = 8.52 KK50 pKa = 4.91 TTTWRR55 pKa = 11.84 MALRR59 pKa = 11.84 RR60 pKa = 11.84 GLNARR65 pKa = 11.84 LPYY68 pKa = 10.31 HH69 pKa = 6.99 LGLPRR74 pKa = 11.84 PVGPYY79 pKa = 9.52 QVIRR83 pKa = 11.84 TTKK86 pKa = 9.98 LHH88 pKa = 5.24 STSARR93 pKa = 11.84 VVVFCPLMQKK103 pKa = 9.86 WNGQDD108 pKa = 3.43 DD109 pKa = 3.85 APRR112 pKa = 11.84 WFEE115 pKa = 3.66 ACGIEE120 pKa = 5.86 DD121 pKa = 3.57 VDD123 pKa = 3.66 ATKK126 pKa = 10.41 PINDD130 pKa = 3.58 ATGNAHH136 pKa = 6.87 MIGMPLTGIGSAAEE150 pKa = 4.03 VVPAAMTVQVMCSDD164 pKa = 4.8 PLQTAQGIFTMGRR177 pKa = 11.84 VSQQLPLGGEE187 pKa = 4.05 TRR189 pKa = 11.84 SWDD192 pKa = 3.55 TFKK195 pKa = 10.32 TQFDD199 pKa = 4.36 AYY201 pKa = 10.05 FKK203 pKa = 10.74 PRR205 pKa = 11.84 LLTGGKK211 pKa = 9.25 LALRR215 pKa = 11.84 GVTCNAIPLNMNEE228 pKa = 4.03 FSEE231 pKa = 4.8 FASPQAAEE239 pKa = 5.38 NNFIWNDD246 pKa = 3.7 TVVPAALSPIVFTKK260 pKa = 10.83 DD261 pKa = 2.88 PGEE264 pKa = 4.29 VGSSITFLVTIEE276 pKa = 3.6 WRR278 pKa = 11.84 VRR280 pKa = 11.84 FDD282 pKa = 3.6 PFHH285 pKa = 6.87 PAAASHH291 pKa = 6.21 TFHH294 pKa = 7.55 PSTPDD299 pKa = 3.36 SLWNRR304 pKa = 11.84 VIGAASSMGHH314 pKa = 5.18 GVEE317 pKa = 5.65 DD318 pKa = 3.61 IADD321 pKa = 3.78 EE322 pKa = 4.24 VAEE325 pKa = 4.62 LGADD329 pKa = 3.34 AAAAAVVGALAFF341 pKa = 4.18
Molecular weight: 36.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.025
IPC2_protein 8.99
IPC_protein 8.96
Toseland 10.028
ProMoST 9.56
Dawson 10.145
Bjellqvist 9.78
Wikipedia 10.248
Rodwell 10.628
Grimsley 10.175
Solomon 10.175
Lehninger 10.175
Nozaki 10.072
DTASelect 9.736
Thurlkill 10.028
EMBOSS 10.409
Sillero 10.072
Patrickios 10.35
IPC_peptide 10.189
IPC2_peptide 8.39
IPC2.peptide.svr19 7.949
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1576
274
961
525.3
57.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.025 ± 0.988
1.396 ± 0.077
5.393 ± 0.595
6.853 ± 1.332
3.871 ± 0.472
9.581 ± 2.098
2.728 ± 1.099
3.68 ± 0.566
4.759 ± 1.537
7.551 ± 0.731
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.728 ± 0.498
2.538 ± 0.401
5.901 ± 0.306
3.68 ± 0.215
7.043 ± 0.316
5.33 ± 0.118
6.028 ± 0.55
7.234 ± 0.573
2.284 ± 0.102
1.396 ± 0.341
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here